Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,289,287 C→A 11.6% P171P (CCG→CCT)  ccmC ← heme exporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,289,2870CA11.6% 1081.9 / 87.6 301P171P (CCG→CCT) ccmCheme exporter subunit
Reads supporting (aligned to +/- strand):  ref base C (122/144);  new base A (16/19);  total (138/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 307 total aligned reads displayed.

TCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACC  >  CP009273/2289139‑2289435
                                                                                                                                                    |                                                                                                                                                    
tccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATc                                                                                                                                                      >  2:1635308/1‑149 (MQ=255)
tccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATc                                                                                                                                                      >  2:614252/1‑149 (MQ=255)
 ccgcatcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCg                                                                                                                                                     >  1:824060/1‑149 (MQ=255)
  cgcatcAGCGTCTGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCgg                                                                                                                                                    <  1:2222264/149‑1 (MQ=255)
    catcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCa                                                                                                                                                  <  1:503004/149‑1 (MQ=255)
    catcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCa                                                                                                                                                  <  2:410866/149‑1 (MQ=255)
     atcAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAg                                                                                                                                                 >  2:2077850/1‑149 (MQ=255)
      tcagcgtcagcgTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGACTAATCGGCAGa                                                                                                                                                >  1:606173/1‑149 (MQ=255)
       cagcgtcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGAt                                                                                                                                               <  2:702437/149‑1 (MQ=255)
       cagcgtcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGAt                                                                                                                                               >  1:3002583/1‑149 (MQ=255)
        agcgtcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGAtt                                                                                                                                              >  1:3488183/1‑149 (MQ=255)
            tcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTcacc                                                                                                                                          >  1:2818005/1‑149 (MQ=255)
            tcagcgtGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTcacc                                                                                                                                          >  2:1538704/1‑149 (MQ=255)
                   ggCAGACAGGAGCAGTAAGCAAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCaa                                                                                                                                   <  1:1365560/149‑1 (MQ=255)
                   ggCAGACAGGAGCAGGAAGCAAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCaa                                                                                                                                   <  1:1330553/149‑1 (MQ=255)
                    gCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGAAACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAAt                                                                                                                                  <  1:3255728/149‑1 (MQ=255)
                      ggACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCa                                                                                                                                <  1:1619932/148‑1 (MQ=255)
                         cAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCa                                                                                                                             <  1:3040651/149‑1 (MQ=255)
                             aGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAg                                                                                                                         <  2:2578910/149‑1 (MQ=255)
                               cAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGa                                                                                                                       <  2:1666090/149‑1 (MQ=255)
                                 ggAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATa                                                                                                                     <  1:1066682/149‑1 (MQ=255)
                                  gAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATAc                                                                                                                    <  1:4528287/149‑1 (MQ=255)
                                  gAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATAc                                                                                                                    <  1:1458858/149‑1 (MQ=255)
                                  gAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATAc                                                                                                                    <  1:4055276/149‑1 (MQ=255)
                                     gCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTg                                                                                                                 <  2:1431071/149‑1 (MQ=255)
                                      ccAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGc                                                                                                                <  1:3384098/149‑1 (MQ=255)
                                      ccAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGc                                                                                                                <  1:1157780/149‑1 (MQ=255)
                                       cAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGcc                                                                                                               >  2:1247674/1‑149 (MQ=255)
                                               gACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGcc                                                                                                       <  2:1815126/149‑1 (MQ=255)
                                                    gcgcagcgGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCa                                                                                                  >  1:4747397/1‑149 (MQ=255)
                                                          cggcgAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGAcggc                                                                                            >  2:1285042/1‑149 (MQ=255)
                                                          cggcgAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGAcggc                                                                                            >  2:2187604/1‑149 (MQ=255)
                                                          cggcgAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGAcggc                                                                                            <  2:4894613/149‑1 (MQ=255)
                                                            gcgAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGAcggcgg                                                                                          >  1:748345/1‑149 (MQ=255)
                                                              gAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGgtc                                                                                        <  2:3502443/149‑1 (MQ=255)
                                                               aaCGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGgtcg                                                                                       >  1:3108501/1‑149 (MQ=255)
                                                                aCGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGgtcgt                                                                                      <  1:4842232/149‑1 (MQ=255)
                                                                aCGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGgtcgt                                                                                      <  2:2112843/149‑1 (MQ=255)
                                                                   cATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGa                                                                                   >  1:1746946/1‑149 (MQ=255)
                                                                    aTCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATACTCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGaa                                                                                  <  2:5189405/149‑1 (MQ=255)
                                                                     tCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGTGGTCGTCGAAg                                                                                 <  1:1417306/149‑1 (MQ=255)
                                                                          ggATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTg                                                                            >  2:4507032/1‑149 (MQ=255)
                                                                             tCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCca                                                                         >  1:3699700/1‑149 (MQ=255)
                                                                              cGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCcac                                                                        <  2:311542/149‑1 (MQ=255)
                                                                               gATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCcaca                                                                       >  1:3305906/1‑149 (MQ=255)
                                                                                aTACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAg                                                                      >  1:3254241/1‑149 (MQ=255)
                                                                                 tACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGTGTGCCACAgg                                                                     >  1:5140957/1‑149 (MQ=255)
                                                                                 tACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAgg                                                                     <  2:2464028/149‑1 (MQ=255)
                                                                                 tACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAgg                                                                     >  1:2187236/1‑149 (MQ=255)
                                                                                  aCTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAggg                                                                    <  2:440822/149‑1 (MQ=255)
                                                                                  aCTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCAACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAggg                                                                    >  2:1482277/1‑149 (MQ=255)
                                                                                   cTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGc                                                                   >  1:698010/1‑149 (MQ=255)
                                                                                     ttGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCATATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCaa                                                                 <  2:1594398/149‑1 (MQ=255)
                                                                                     ttGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCaa                                                                 <  2:3918511/149‑1 (MQ=255)
                                                                                       gctgcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATc                                                               <  2:833759/149‑1 (MQ=255)
                                                                                       gctgcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATc                                                               <  2:2740950/149‑1 (MQ=255)
                                                                                        ctgcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAAtaa                                                              <  1:4789118/149‑3 (MQ=255)
                                                                                          gcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATaaca                                                            <  2:4926814/149‑1 (MQ=255)
                                                                                          gcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATaaca                                                            >  2:4334608/1‑149 (MQ=255)
                                                                                            aTCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACAcc                                                          >  1:4573180/1‑149 (MQ=255)
                                                                                            aTCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAcc                                                          >  1:2039455/1‑149 (MQ=255)
                                                                                            aTCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACAcc                                                          <  2:1007781/149‑1 (MQ=255)
                                                                                            aTCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACAcc                                                          <  2:3807285/149‑1 (MQ=255)
                                                                                             tCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAccc                                                         >  2:3007934/1‑149 (MQ=255)
                                                                                              ccGAGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAAGGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCca                                                        <  2:3191660/149‑1 (MQ=255)
                                                                                                   ttGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATAc                                                   >  1:4406586/1‑149 (MQ=255)
                                                                                                   ttGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATAc                                                   <  1:4962714/149‑1 (MQ=255)
                                                                                                     gATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaa                                                 <  1:5153137/149‑1 (MQ=255)
                                                                                                      aTCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACaaa                                                >  1:3740349/1‑149 (MQ=255)
                                                                                                         ccTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAAc                                             <  2:120049/149‑1 (MQ=255)
                                                                                                            gATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAAcagc                                          <  1:3175035/149‑1 (MQ=255)
                                                                                                             aTGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcagca                                         <  2:243655/149‑1 (MQ=255)
                                                                                                              tGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAAcagcag                                        >  1:1654326/1‑149 (MQ=255)
                                                                                                              tGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAAcagcag                                        >  1:4250763/1‑149 (MQ=255)
                                                                                                                  gggTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCAcc                                    <  1:1916588/149‑1 (MQ=255)
                                                                                                                     tgtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGt                                 <  1:2627271/149‑1 (MQ=255)
                                                                                                                       ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTc                               <  2:3368060/149‑1 (MQ=255)
                                                                                                                       ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTc                               <  2:898408/149‑1 (MQ=255)
                                                                                                                       ttCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTc                               <  2:2828061/149‑1 (MQ=255)
                                                                                                                          caccacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGa                            <  2:3742206/149‑1 (MQ=255)
                                                                                                                           accacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGaa                           <  2:2012097/149‑1 (MQ=255)
                                                                                                                            ccacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAg                          >  1:4112956/1‑149 (MQ=255)
                                                                                                                             cacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                         <  1:1233049/149‑1 (MQ=255)
                                                                                                                              acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTc                        >  1:3229908/1‑149 (MQ=255)
                                                                                                                              acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTc                        <  2:1379494/149‑1 (MQ=255)
                                                                                                                              acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCTGAAGTc                        >  2:1319266/1‑149 (MQ=255)
                                                                                                                              acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTc                        <  2:2922359/149‑1 (MQ=255)
                                                                                                                               cTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCa                       >  2:3647206/1‑149 (MQ=255)
                                                                                                                                 ccACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGa                     >  2:578150/1‑149 (MQ=255)
                                                                                                                                   aCGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACg                   >  1:1532276/1‑149 (MQ=255)
                                                                                                                                       aGTATTGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCa               <  1:3087505/149‑1 (MQ=255)
                                                                                                                                          aaTGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATcc            >  2:1556916/1‑149 (MQ=255)
                                                                                                                                           aTGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATccc           >  2:1164971/1‑149 (MQ=255)
                                                                                                                                           aTGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATccc           >  1:3910467/1‑149 (MQ=255)
                                                                                                                                             gAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCAt         >  2:2223392/1‑149 (MQ=255)
                                                                                                                                                 aatCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATAccc     <  1:515864/149‑1 (MQ=255)
                                                                                                                                                  atCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACcca    >  1:3533605/1‑149 (MQ=255)
                                                                                                                                                   tCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccac   >  1:5066216/1‑149 (MQ=255)
                                                                                                                                                   tCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccac   >  2:4243806/1‑149 (MQ=255)
                                                                                                                                                   tCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccac   >  1:4775959/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCCGCATCAGCGTCAGCGTGGCAGACAGGAGCAGGAAGCCAAAAATCGACCAGCGCAGCGGCGAACGCATCGCCGGATCGATACTTTGCTGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACC  >  CP009273/2289139‑2289435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑