Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,530,470 C→A 5.0% G43C (GGC→TGC)  pdxK ← pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,530,4700CA 5.0% 1238.9 / 30.3 317G43C (GGC→TGC) pdxKpyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base C (169/130);  new base A (10/7);  total (179/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

CAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTCCTTGCCGAT  >  CP009273/2530323‑2530618
                                                                                                                                                   |                                                                                                                                                    
cAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCg                                                                                                                                                     >  1:4377157/1‑149 (MQ=255)
 aGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGt                                                                                                                                                    >  1:345236/1‑149 (MQ=255)
  gCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGtt                                                                                                                                                   >  1:4925494/1‑149 (MQ=255)
   cACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTc                                                                                                                                                  <  1:3236429/149‑1 (MQ=255)
   cACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTc                                                                                                                                                  <  2:2535332/149‑1 (MQ=255)
    aCGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCt                                                                                                                                                 >  2:2346882/1‑149 (MQ=255)
      gAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGt                                                                                                                                               <  2:3782498/149‑1 (MQ=255)
        aGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGttt                                                                                                                                             >  1:988596/1‑149 (MQ=255)
           tGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGAt                                                                                                                                          <  2:3752435/149‑1 (MQ=255)
             gcgcagcgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAg                                                                                                                                        <  1:816820/149‑1 (MQ=255)
              cgcagcgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGc                                                                                                                                       <  2:1414230/149‑1 (MQ=255)
               gcagcgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCa                                                                                                                                      <  1:4050573/149‑1 (MQ=255)
               gcagcgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCa                                                                                                                                      <  1:4050669/149‑1 (MQ=255)
                cagcgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAg                                                                                                                                     <  1:2377646/149‑1 (MQ=255)
                  gcgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGc                                                                                                                                   >  1:2960975/1‑149 (MQ=255)
                   cgcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCa                                                                                                                                  <  1:2823894/149‑1 (MQ=255)
                    gcaTCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGGTCTGTTTGATAGCAGGCAc                                                                                                                                 >  2:3685426/1‑149 (MQ=255)
                       tCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGc                                                                                                                              >  1:991283/1‑149 (MQ=255)
                        cACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCa                                                                                                                             >  2:2236589/1‑149 (MQ=255)
                         aCGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCaa                                                                                                                            >  2:684021/1‑149 (MQ=255)
                            cTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGc                                                                                                                         >  1:1359648/1‑149 (MQ=255)
                            cTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGc                                                                                                                         >  1:1626760/1‑149 (MQ=255)
                              ccTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTg                                                                                                                       <  2:2969987/149‑1 (MQ=255)
                              ccTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATAGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGACAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTg                                                                                                                       >  1:854203/1‑149 (MQ=255)
                               cTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGt                                                                                                                      <  2:443000/149‑1 (MQ=255)
                                 gAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTg                                                                                                                    >  2:1253119/1‑149 (MQ=255)
                                      gcacgcaAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCAc                                                                                                               >  1:3926515/1‑149 (MQ=255)
                                        acgcaAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGc                                                                                                             >  1:546388/1‑149 (MQ=255)
                                         cgcaAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCt                                                                                                            <  2:3515195/149‑1 (MQ=255)
                                           caAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGc                                                                                                          >  1:4784556/1‑149 (MQ=255)
                                           caAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGc                                                                                                          >  1:2549744/1‑149 (MQ=255)
                                              aTAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGt                                                                                                       >  2:4315932/1‑149 (MQ=255)
                                                aGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTaa                                                                                                     >  1:1239634/1‑149 (MQ=255)
                                                     cTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAaccac                                                                                                <  2:3707002/149‑1 (MQ=255)
                                                         aCCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGc                                                                                            >  1:2135149/1‑149 (MQ=255)
                                                            aTTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGAc                                                                                         >  2:2664170/1‑149 (MQ=255)
                                                            aTTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGAc                                                                                         >  2:4697351/1‑149 (MQ=255)
                                                                gTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCa                                                                                     >  1:4636870/1‑149 (MQ=255)
                                                                 tCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCAc                                                                                    <  1:3173661/149‑1 (MQ=255)
                                                                 tCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCAc                                                                                    >  1:264507/1‑149 (MQ=255)
                                                                   cGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACgg                                                                                  >  2:681641/1‑149 (MQ=255)
                                                                         cGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGcgacga                                                                            <  2:2203114/149‑1 (MQ=255)
                                                                          gCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGat                                                                           <  1:1526033/149‑1 (MQ=255)
                                                                          gCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACGGCACGGCGACGat                                                                           <  2:4208760/149‑1 (MQ=255)
                                                                             ccaccGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACGGCACGGCGACGATATc                                                                        <  2:3491351/149‑1 (MQ=255)
                                                                                ccGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGc                                                                     <  1:1611748/149‑1 (MQ=255)
                                                                                ccGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGc                                                                     >  2:3239244/1‑149 (MQ=255)
                                                                                 cGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCAGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGcc                                                                    <  2:2077227/149‑1 (MQ=255)
                                                                                   tAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTg                                                                  >  2:4269514/1‑149 (MQ=255)
                                                                                    aGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGc                                                                 <  1:1191836/149‑1 (MQ=255)
                                                                                     gAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCa                                                                <  1:3509482/149‑1 (MQ=255)
                                                                                       aaGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGt                                                              >  1:1293585/1‑149 (MQ=255)
                                                                                       aaGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGt                                                              >  1:4457877/1‑149 (MQ=255)
                                                                                       aaGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGt                                                              >  2:2721418/1‑149 (MQ=255)
                                                                                         gtgtCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGc                                                            >  2:1475858/1‑149 (MQ=255)
                                                                                         gtgtCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGc                                                            >  2:397841/1‑149 (MQ=255)
                                                                                         gtgtCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGc                                                            >  1:3808746/1‑149 (MQ=255)
                                                                                              aTAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCAGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTAc                                                       >  2:4628232/1‑149 (MQ=255)
                                                                                                 aTGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTActct                                                    >  1:659302/1‑149 (MQ=255)
                                                                                                 aTGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTActct                                                    >  1:1083568/1‑149 (MQ=255)
                                                                                                 aTGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTActct                                                    <  1:4499170/149‑1 (MQ=255)
                                                                                                   gcggcgTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTa                                                  >  2:5199914/1‑149 (MQ=255)
                                                                                                       cgTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGt                                              <  1:2724240/149‑1 (MQ=255)
                                                                                                        gTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCAGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGtt                                             >  2:4988206/1‑149 (MQ=255)
                                                                                                        gTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCAGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGtt                                             >  2:2386049/1‑149 (MQ=255)
                                                                                                            tGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAaac                                         >  2:5084663/1‑149 (MQ=255)
                                                                                                            tGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAaac                                         <  1:1957703/149‑1 (MQ=255)
                                                                                                            tGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAaac                                         <  2:1916135/149‑1 (MQ=255)
                                                                                                               tCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAaacaac                                      <  2:2436009/149‑1 (MQ=255)
                                                                                                               tCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAaacaac                                      >  2:5151044/1‑149 (MQ=255)
                                                                                                               tCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAaacaac                                      <  2:3911751/149‑1 (MQ=255)
                                                                                                               tCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAaacaac                                      <  2:3863131/149‑1 (MQ=255)
                                                                                                                   cAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAacaacaaca                                  <  2:2812131/149‑1 (MQ=255)
                                                                                                                    aaTACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAAcaacaacaa                                 <  2:4819397/149‑1 (MQ=255)
                                                                                                                    aaTACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAAcaacaacaa                                 <  2:2005198/149‑1 (MQ=255)
                                                                                                                     aTACCGTCGGCACGGCAAAGACATTCAGGCAGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACaaa                                >  2:775927/1‑149 (MQ=255)
                                                                                                                       aCCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAact                              <  1:3384259/149‑1 (MQ=255)
                                                                                                                       aCCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAact                              <  2:1694084/149‑1 (MQ=255)
                                                                                                                       aCCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAact                              >  2:4566000/1‑149 (MQ=255)
                                                                                                                            cggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCa                         <  1:4197986/149‑1 (MQ=255)
                                                                                                                             ggcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCAt                        >  1:2251584/1‑149 (MQ=255)
                                                                                                                              gcacggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCAtt                       >  1:3122276/1‑149 (MQ=255)
                                                                                                                                acggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTa                     <  2:4777546/149‑1 (MQ=255)
                                                                                                                                acggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTa                     >  2:4939565/1‑149 (MQ=255)
                                                                                                                                 cggcaAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTaa                    <  1:64875/149‑1 (MQ=255)
                                                                                                                                     aaaGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAAtttt                >  1:554406/1‑149 (MQ=255)
                                                                                                                                     aaaGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAAtttt                >  1:1723626/1‑149 (MQ=255)
                                                                                                                                     aaaGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAAtttt                <  2:316103/149‑1 (MQ=255)
                                                                                                                                       aGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAAtttttt              <  1:3894302/149‑1 (MQ=255)
                                                                                                                                        gACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTtc             >  1:1836002/1‑149 (MQ=255)
                                                                                                                                        gACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTtc             >  1:1471746/1‑149 (MQ=255)
                                                                                                                                        gACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATACCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTtc             <  1:2431780/149‑1 (MQ=255)
                                                                                                                                          cATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTtctc           <  2:3405342/149‑1 (MQ=255)
                                                                                                                                          cATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTtctc           >  1:715817/1‑149 (MQ=255)
                                                                                                                                           aTTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTcc          >  1:4059172/1‑149 (MQ=255)
                                                                                                                                           aTTCAGGCCGGTCTGTTTGATAGCAGGCACGGCAATGCTGGTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTcc          <  2:345236/149‑1 (MQ=255)
                                                                                                                                              cAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTCCTTg       >  2:2124201/1‑149 (MQ=255)
                                                                                                                                               aGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTCCTTGc      <  2:4526385/149‑1 (MQ=255)
                                                                                                                                                ggCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTCCTTGcc     <  2:485555/149‑1 (MQ=255)
                                                                                                                                                   cGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTCCTTGCCgat  <  1:3530439/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGCACGAAGTTGGCGCAGCGCATCACGCTCCTGAAGCGCACGCAAATAGCCGCTAAACCATTCGTCCGGAATCGCACCACCGTAGAAAGTGTCATAATGCGGCGTATTGCTCAGCAATACCGTCGGCACGGCAAAGACATTCAGGCCGTTCTGTTTGATAGCAGGCACGGCAATGCTGTTGCCCACGCTGCCGTAAACCACCTGCGACTGCACGGCGACGATATCCGCCTGCAGTGCCCTACTCTTATCGTTAAACAACAACAAACTACTCATTTAATTTTTTCTCCTTGCCGAT  >  CP009273/2530323‑2530618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑