Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,609,187 C→A 25.6% A18A (GCG→GCT)  yfgO ← putative UPF0118 family inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,609,1870CA25.6% 797.2 / 239.8 330A18A (GCG→GCT) yfgOputative UPF0118 family inner membrane permease
Reads supporting (aligned to +/- strand):  ref base C (126/119);  new base A (46/39);  total (172/158)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

GCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGATTCAGGA  >  CP009273/2609040‑2609334
                                                                                                                                                   |                                                                                                                                                   
 cggcggGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGc                                                                                                                                                   <  1:4726148/149‑1 (MQ=255)
  ggcggGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCt                                                                                                                                                  <  2:2708749/149‑1 (MQ=255)
  ggcggGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCt                                                                                                                                                  <  2:3168017/149‑1 (MQ=255)
    cggGAGCAGTCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTc                                                                                                                                                <  1:5150398/149‑1 (MQ=255)
    cggGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTc                                                                                                                                                <  2:4978998/149‑1 (MQ=255)
          cagcCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTc                                                                                                                                          >  2:3119193/1‑149 (MQ=255)
                 tAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTaaaa                                                                                                                                   <  1:5019656/149‑1 (MQ=255)
                 tAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTaaaa                                                                                                                                   <  2:1860314/149‑1 (MQ=255)
                 tAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTaaaa                                                                                                                                   <  2:2975397/149‑1 (MQ=255)
                 tAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTaaaa                                                                                                                                   <  2:142057/149‑1 (MQ=255)
                  aGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAAc                                                                                                                                  <  2:91988/149‑1 (MQ=255)
                    aTTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAAcgg                                                                                                                                <  1:47078/149‑1 (MQ=255)
                     ttGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAAcggc                                                                                                                               >  2:33896/1‑149 (MQ=255)
                          gACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGa                                                                                                                          >  1:1221615/1‑149 (MQ=255)
                             gCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATAc                                                                                                                       >  1:3827547/1‑149 (MQ=255)
                             gCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATAc                                                                                                                       >  2:1621589/1‑149 (MQ=255)
                                 ggTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCAtt                                                                                                                   <  1:3993274/149‑1 (MQ=255)
                                    tGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCa                                                                                                                <  2:813335/149‑1 (MQ=255)
                                     ggCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCAGTGCAt                                                                                                               >  1:1750179/1‑149 (MQ=255)
                                      gCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATc                                                                                                              >  2:3444091/1‑149 (MQ=255)
                                      gCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATc                                                                                                              <  1:2035716/149‑1 (MQ=255)
                                        cATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCaa                                                                                                            <  1:34263/149‑1 (MQ=255)
                                         aTTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAAc                                                                                                           >  1:3782434/1‑149 (MQ=255)
                                             cAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACAttt                                                                                                       >  2:4288441/1‑149 (MQ=255)
                                              aGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTc                                                                                                      >  1:1536532/1‑149 (MQ=255)
                                              aGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTc                                                                                                      >  1:4749953/1‑149 (MQ=255)
                                                 aaaTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAg                                                                                                   <  2:3961300/149‑1 (MQ=255)
                                                  aaTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGc                                                                                                  >  1:30524/1‑149 (MQ=255)
                                                  aaTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAAAATTTCGAGc                                                                                                  >  1:1568443/1‑149 (MQ=255)
                                                  aaTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGAAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGc                                                                                                  <  2:1307834/149‑1 (MQ=255)
                                                   aTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCa                                                                                                 <  2:1681501/149‑1 (MQ=255)
                                                            cacaATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCt                                                                                        <  2:3594970/149‑1 (MQ=255)
                                                            cacaATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCt                                                                                        >  2:1043173/1‑149 (MQ=255)
                                                                aTAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTccc                                                                                    <  2:4241614/149‑1 (MQ=255)
                                                                aTAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTccc                                                                                    <  1:673183/149‑1 (MQ=255)
                                                                  aGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTg                                                                                  >  2:3899716/1‑149 (MQ=255)
                                                                     caccaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAAc                                                                               <  1:3602841/149‑1 (MQ=255)
                                                                     caccaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAAc                                                                               >  2:4496207/1‑149 (MQ=255)
                                                                      accaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAAcc                                                                              >  2:2243462/1‑149 (MQ=255)
                                                                      accaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAAcc                                                                              >  2:4839285/1‑149 (MQ=255)
                                                                       ccaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCt                                                                             >  1:5124121/1‑149 (MQ=255)
                                                                         aGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCa                                                                           >  2:1243292/1‑149 (MQ=255)
                                                                            aaCGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAaga                                                                        <  1:805739/149‑1 (MQ=255)
                                                                             aCGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAagag                                                                       >  1:937934/1‑149 (MQ=255)
                                                                                gAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCgg                                                                    >  1:1920694/1‑149 (MQ=255)
                                                                                gAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCgg                                                                    >  2:1498153/1‑149 (MQ=255)
                                                                                 agcaagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCggg                                                                   <  2:4032888/149‑1 (MQ=255)
                                                                                   caagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAACTAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGAt                                                                 <  2:3582338/149‑1 (MQ=255)
                                                                                   caagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGAt                                                                 <  1:579705/149‑1 (MQ=255)
                                                                                    aagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGAtt                                                                <  2:3306014/149‑1 (MQ=255)
                                                                                    aagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGAtt                                                                <  2:3782502/149‑1 (MQ=255)
                                                                                    aagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGAtt                                                                >  1:2936179/1‑149 (MQ=255)
                                                                                     agcaGGCCACTAAAGAAAAAGATAATGCCAACTCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAACGACCTTCCCTGAACCTCAAGAGCGGGATTg                                                               <  2:1584028/149‑1 (MQ=255)
                                                                                      gcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGc                                                              >  2:4002521/1‑149 (MQ=255)
                                                                                            cACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCg                                                        >  1:518439/1‑149 (MQ=255)
                                                                                            cACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCg                                                        >  2:4559559/1‑149 (MQ=255)
                                                                                             aCTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGc                                                       <  1:4576156/149‑1 (MQ=255)
                                                                                             aCTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCAACAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGc                                                       <  1:721976/149‑1 (MQ=255)
                                                                                             aCTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGc                                                       >  2:3100511/1‑149 (MQ=255)
                                                                                              cTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCa                                                      >  1:1102904/1‑149 (MQ=255)
                                                                                               tAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCaa                                                     >  1:1638275/1‑149 (MQ=255)
                                                                                                aaaGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAAt                                                    <  1:1667104/149‑1 (MQ=255)
                                                                                                  aGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTg                                                  <  2:4086055/149‑1 (MQ=255)
                                                                                                  aGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTg                                                  <  2:5120148/149‑1 (MQ=255)
                                                                                                    aaaaaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTa                                                <  2:590954/149‑1 (MQ=255)
                                                                                                    aaaaaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTa                                                >  1:2804391/1‑149 (MQ=255)
                                                                                                     aaaaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTAt                                               <  1:4256460/149‑1 (MQ=255)
                                                                                                      aaaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATc                                              <  2:4369612/149‑1 (MQ=255)
                                                                                                       aaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCg                                             >  1:1872195/1‑149 (MQ=255)
                                                                                                       aaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCg                                             >  1:4466491/1‑149 (MQ=255)
                                                                                                         gATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGaa                                           >  2:2114553/1‑149 (MQ=255)
                                                                                                          aTAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGaaa                                          <  2:2505948/149‑1 (MQ=255)
                                                                                                           tAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAAt                                         >  1:4201412/1‑149 (MQ=255)
                                                                                                           tAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAAt                                         >  1:1052915/1‑149 (MQ=255)
                                                                                                           tAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAAt                                         <  1:4162155/149‑1 (MQ=255)
                                                                                                            aaTGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATg                                        <  1:4089135/149‑1 (MQ=255)
                                                                                                            aaTGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAAAGGCGGCGATACCATTTCATAAACATTTAGAGCACAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGaaaag                                        >  1:1926439/1‑147 (MQ=255)
                                                                                                              tGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTc                                      >  1:391550/1‑149 (MQ=255)
                                                                                                              tGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTc                                      <  2:1222295/149‑1 (MQ=255)
                                                                                                                  aaaTCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaa                                  >  1:4506229/1‑149 (MQ=255)
                                                                                                                   aaTCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaaa                                 >  2:4875923/1‑149 (MQ=255)
                                                                                                                        ggCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGa                            <  1:1682764/149‑1 (MQ=255)
                                                                                                                        ggCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATAGAAATGTCACAAAAAAGa                            >  2:2827610/1‑149 (MQ=255)
                                                                                                                          cAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACt                          <  1:868839/149‑1 (MQ=255)
                                                                                                                           aaCTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACtt                         <  2:176494/149‑1 (MQ=255)
                                                                                                                            aCTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTc                        <  2:2359424/149‑1 (MQ=255)
                                                                                                                                 aaTAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCttt                   <  2:2007062/149‑1 (MQ=255)
                                                                                                                                 aaTAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCttt                   <  1:26089/149‑1 (MQ=255)
                                                                                                                                  aTAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCtttt                  <  1:3119193/149‑1 (MQ=255)
                                                                                                                                  aTAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCtttt                  >  1:2267837/1‑149 (MQ=255)
                                                                                                                                   tAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCttttt                 <  1:3232628/149‑1 (MQ=255)
                                                                                                                                   tAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATAGAAATGTCACAAAAAAGACGTCGCttttt                 >  1:4392349/1‑149 (MQ=255)
                                                                                                                                    aaCCAGCAAGGCTATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTa                <  2:499278/149‑1 (MQ=255)
                                                                                                                                    aaCCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTa                >  1:2498604/1‑149 (MQ=255)
                                                                                                                                     aCCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTAt               >  2:2681634/1‑149 (MQ=255)
                                                                                                                                        aGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGAc            >  2:5056346/1‑149 (MQ=255)
                                                                                                                                           aaGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCCATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGa         >  1:1365283/1‑149 (MQ=255)
                                                                                                                                             ggCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGAtt       <  2:878451/149‑1 (MQ=255)
                                                                                                                                                aaTCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGATTCAg    >  1:3311742/1‑149 (MQ=255)
                                                                                                                                                  tCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGATTCAGGa  <  2:2434511/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGATTCAGGA  >  CP009273/2609040‑2609334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑