Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,679,933 C→A 13.2% A247D (GCT→GAT)  hmp → fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,679,9330CA13.2% 1165.2 / 121.4 340A247D (GCT→GAT) hmpfused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand):  ref base C (164/131);  new base A (28/17);  total (192/148)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 345 total aligned reads displayed.

TTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCATGCGGCA  >  CP009273/2679785‑2680081
                                                                                                                                                    |                                                                                                                                                    
ttCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGc                                                                                                                                                      <  1:2861992/149‑1 (MQ=255)
  cccACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTc                                                                                                                                                    <  1:3436125/149‑1 (MQ=255)
   ccACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTcc                                                                                                                                                   <  2:3362638/149‑1 (MQ=255)
      cctCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTAGTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGc                                                                                                                                                <  2:225418/147‑1 (MQ=255)
        tCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAg                                                                                                                                              <  1:24590/149‑1 (MQ=255)
          aGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGt                                                                                                                                            >  2:791027/1‑149 (MQ=255)
          aGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGt                                                                                                                                            <  1:1080016/149‑1 (MQ=255)
             aaaTTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGAt                                                                                                                                         >  1:3077210/1‑149 (MQ=255)
               aTTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGAttt                                                                                                                                       <  1:5062848/149‑1 (MQ=255)
                  cGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCtt                                                                                                                                    >  1:75703/1‑149 (MQ=255)
                    tCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTa                                                                                                                                  <  2:2358121/149‑1 (MQ=255)
                    tCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTa                                                                                                                                  >  2:5156845/1‑149 (MQ=255)
                     cAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTAt                                                                                                                                 <  2:897861/149‑1 (MQ=255)
                         aCTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCt                                                                                                                             <  1:2244275/149‑1 (MQ=255)
                           tctTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGt                                                                                                                           <  2:1899315/149‑1 (MQ=255)
                           tctTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGt                                                                                                                           <  2:787799/149‑1 (MQ=255)
                                 aCTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGa                                                                                                                     >  2:4416251/1‑149 (MQ=255)
                                   tCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATg                                                                                                                   >  2:4643959/1‑149 (MQ=255)
                                      cAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGaca                                                                                                                >  1:4393583/1‑149 (MQ=255)
                                       aaaCCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGacac                                                                                                               >  1:3359720/1‑149 (MQ=255)
                                        aaCCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGacaca                                                                                                              >  2:3495764/1‑149 (MQ=255)
                                        aaCCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGacaca                                                                                                              >  2:2304213/1‑149 (MQ=255)
                                        aaCCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGacaca                                                                                                              >  2:1186197/1‑149 (MQ=255)
                                                   aaaGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGtt                                                                                                   <  1:3858117/149‑1 (MQ=255)
                                                   aaaGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGtt                                                                                                   <  1:522883/149‑1 (MQ=255)
                                                   aaaGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGtt                                                                                                   >  2:61474/1‑149 (MQ=255)
                                                    aaGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCACTGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTa                                                                                                  >  2:2821641/1‑149 (MQ=255)
                                                     aGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTaa                                                                                                 >  2:908568/1‑149 (MQ=255)
                                                     aGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTaa                                                                                                 >  2:2560248/1‑149 (MQ=255)
                                                     aGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTaa                                                                                                 <  1:3554551/149‑1 (MQ=255)
                                                      ggCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAAt                                                                                                >  1:3713860/1‑149 (MQ=255)
                                                      ggCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAAt                                                                                                >  1:3713902/1‑149 (MQ=255)
                                                      ggCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAAt                                                                                                >  1:1163120/1‑149 (MQ=255)
                                                      ggCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGATGTAGCAGATGACACACCAGTGACGTTAAt                                                                                                >  2:3126734/1‑149 (MQ=255)
                                                        cTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAAtct                                                                                              <  1:3137185/149‑1 (MQ=255)
                                                         tATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAAtctc                                                                                             <  1:1411004/149‑1 (MQ=255)
                                                            cGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCATGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGc                                                                                          <  1:1617894/149‑1 (MQ=255)
                                                             gTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGcc                                                                                         <  2:1934218/149‑1 (MQ=255)
                                                               aTTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCgg                                                                                       >  1:3200351/1‑149 (MQ=255)
                                                                ttGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGgt                                                                                      >  1:1014467/1‑149 (MQ=255)
                                                                    ggTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTg                                                                                  <  2:558530/149‑1 (MQ=255)
                                                                      tGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGt                                                                                <  2:1595255/149‑1 (MQ=255)
                                                                      tGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGt                                                                                <  2:4100075/149‑1 (MQ=255)
                                                                         aaCGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCaa                                                                             >  1:3005956/1‑149 (MQ=255)
                                                                         aaCGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCaa                                                                             >  1:2378166/1‑149 (MQ=255)
                                                                         aaCGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCaa                                                                             >  1:3939031/1‑149 (MQ=255)
                                                                            gcgAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACg                                                                          >  1:902700/1‑149 (MQ=255)
                                                                            gcgAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACg                                                                          >  1:859251/1‑149 (MQ=255)
                                                                               aaGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCa                                                                       >  2:4560923/1‑149 (MQ=255)
                                                                               aaGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCa                                                                       >  2:270374/1‑149 (MQ=255)
                                                                               aaGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCa                                                                       >  1:199032/1‑149 (MQ=255)
                                                                                  agGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATg                                                                    >  1:4102643/1‑149 (MQ=255)
                                                                                   gggTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGc                                                                   <  2:1394872/149‑1 (MQ=255)
                                                                                    ggTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCt                                                                  <  1:5077035/149‑1 (MQ=255)
                                                                                     gTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTg                                                                 <  1:1484048/149‑1 (MQ=255)
                                                                                        ggCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCa                                                              <  2:3814829/149‑1 (MQ=255)
                                                                                         gCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCaa                                                             <  1:2819592/149‑1 (MQ=255)
                                                                                          cAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAAt                                                            >  2:4835746/1‑149 (MQ=255)
                                                                                           aGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATg                                                           >  2:3939984/1‑149 (MQ=255)
                                                                                           aGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATg                                                           >  1:1841816/1‑149 (MQ=255)
                                                                                            ggTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGc                                                          >  1:4054004/1‑149 (MQ=255)
                                                                                             gTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCt                                                         >  1:1978482/1‑149 (MQ=255)
                                                                                              tATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTc                                                        >  2:1777669/1‑149 (MQ=255)
                                                                                              tATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTc                                                        <  2:479298/149‑1 (MQ=255)
                                                                                               aTCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCg                                                       >  1:4967639/1‑149 (MQ=255)
                                                                                                tCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGa                                                      <  2:4751906/149‑1 (MQ=255)
                                                                                                  cAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGaca                                                    <  1:1675586/149‑1 (MQ=255)
                                                                                                  cAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGaca                                                    <  1:2744347/149‑1 (MQ=255)
                                                                                                     cTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGc                                                 >  2:1128483/1‑149 (MQ=255)
                                                                                                     cTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGc                                                 >  2:3608319/1‑149 (MQ=255)
                                                                                                      tGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCt                                                >  1:4846265/1‑149 (MQ=255)
                                                                                                      tGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCt                                                <  1:3869707/149‑1 (MQ=255)
                                                                                                       ggTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTg                                               <  1:3536621/149‑1 (MQ=255)
                                                                                                             acaATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCaaaa                                         >  2:4312071/1‑149 (MQ=255)
                                                                                                              caATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCACAAg                                        >  2:1313133/1‑149 (MQ=255)
                                                                                                               aaTCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGc                                       <  2:4476923/149‑1 (MQ=255)
                                                                                                                aTCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCa                                      >  1:2620767/1‑149 (MQ=255)
                                                                                                                  cACGCCAATGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAgg                                    >  2:1734802/1‑149 (MQ=255)
                                                                                                                    cGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGcc                                  <  1:1685923/149‑1 (MQ=255)
                                                                                                                     gCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCca                                 <  1:5020782/149‑1 (MQ=255)
                                                                                                                         aTGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCcacaca                             >  1:1727902/1‑149 (MQ=255)
                                                                                                                         aTGTTGGCGATGTCGTGAAACTGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCcacaca                             >  1:3968651/1‑149 (MQ=255)
                                                                                                                            ttGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGca                          >  1:619476/1‑149 (MQ=255)
                                                                                                                                cGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAg                      <  2:3283460/149‑1 (MQ=255)
                                                                                                                                  aTGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTg                    >  2:1061666/1‑149 (MQ=255)
                                                                                                                                  aTGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTg                    >  1:4554576/1‑149 (MQ=255)
                                                                                                                                    gTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGaa                  >  2:2136844/1‑149 (MQ=255)
                                                                                                                                     tCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAAc                 <  2:1539188/149‑1 (MQ=255)
                                                                                                                                     tCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAAc                 <  1:2116300/149‑1 (MQ=255)
                                                                                                                                       gTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTg               >  2:2010421/1‑149 (MQ=255)
                                                                                                                                       gTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTg               >  2:3078932/1‑149 (MQ=255)
                                                                                                                                        tGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTgg              >  2:3117590/1‑149 (MQ=255)
                                                                                                                                          aaaCTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGtt            <  2:4544511/149‑1 (MQ=255)
                                                                                                                                             cTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCa         <  1:3672565/149‑1 (MQ=255)
                                                                                                                                              tGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCAt        >  2:2321157/1‑149 (MQ=255)
                                                                                                                                              tGGTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCAt        >  2:4693960/1‑149 (MQ=255)
                                                                                                                                               ggTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCATg       >  2:426063/1‑149 (MQ=255)
                                                                                                                                                gTCGATCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGAAGGCCACACAGCACAAGTGAACTGGTTCCATGc      >  1:3220843/1‑149 (MQ=255)
                                                                                                                                                  cGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCATGCgg    <  2:4909346/149‑1 (MQ=255)
                                                                                                                                                  cGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCATGCgg    >  2:1292550/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCATGCGGCA  >  CP009273/2679785‑2680081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑