BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,793,020	C→T	6.1%	E112K (GAA→AAA)	ygaC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,793,020	0	C	T	6.1%	1102.5 / 39.5	313	E112K (GAA→AAA)	ygaC	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (140/152); new base T (12/7); total (154/159)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 316 total aligned reads displayed.

ACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCC  >  CP009273/2792872‑2793168

 cATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCg                                                                                                                                                     >  1:4701694/1‑149 (MQ=255)

      ttgttTCGATGTTGTTGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACGGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCaaa                                                                                                                                                <  1:3674801/149‑1 (MQ=255)

        gttTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATa                                                                                                                                              >  2:3340509/1‑149 (MQ=255)

         tttCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaa                                                                                                                                             >  1:3015476/1‑149 (MQ=255)

           tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc                                                                                                                                           <  2:4769475/149‑1 (MQ=255)

           tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAAATGGCTTCGCCAAATAAAc                                                                                                                                           >  1:4911516/1‑149 (MQ=255)

             gATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTATTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCa                                                                                                                                         <  1:1308356/149‑1 (MQ=255)

              aTGTTGATGGGGGCGTTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCTGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAt                                                                                                                                        <  1:3460866/149‑1 (MQ=255)

              aTGTTGATGGGGGCGGTTTTTGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACGGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAt                                                                                                                                        <  1:1054234/149‑1 (MQ=255)

              aTGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGTTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAt                                                                                                                                        <  1:1961159/149‑1 (MQ=255)

              aTGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAt                                                                                                                                        >  1:3297126/1‑149 (MQ=255)

               tGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAtt                                                                                                                                       <  2:1439662/149‑1 (MQ=255)

                gTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAATc                                                                                                                                      >  1:4064041/1‑149 (MQ=255)

                 ttGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCa                                                                                                                                     <  2:2771262/149‑1 (MQ=255)

                  tGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCaa                                                                                                                                    <  2:2489765/149‑1 (MQ=255)

                  tGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCaa                                                                                                                                    <  2:361573/149‑1 (MQ=255)

                   gATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCaaa                                                                                                                                   <  2:367922/149‑1 (MQ=255)

                    aTGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAAt                                                                                                                                  >  2:233471/1‑149 (MQ=255)

                     tGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATa                                                                                                                                 >  2:2816225/1‑149 (MQ=255)

                     tGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTATTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATa                                                                                                                                 <  1:2672034/149‑1 (MQ=255)

                         ggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGt                                                                                                                             <  2:709840/149‑1 (MQ=255)

                          gCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGtt                                                                                                                            >  2:792299/1‑149 (MQ=255)

                              tttATGGCTACGTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAg                                                                                                                        <  1:1111808/149‑1 (MQ=255)

                               ttATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAgc                                                                                                                       <  2:5054825/149‑1 (MQ=255)

                                 aTGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAgcgc                                                                                                                     >  2:3869385/1‑149 (MQ=255)

                                  tGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCa                                                                                                                    >  2:2990242/1‑149 (MQ=255)

                                   ggCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCaa                                                                                                                   >  1:806420/1‑149 (MQ=255)

                                     cTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAaca                                                                                                                 <  2:2713331/149‑1 (MQ=255)

                                     cTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAaca                                                                                                                 <  1:3639669/149‑1 (MQ=255)

                                      tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAacac                                                                                                                <  1:1186440/149‑1 (MQ=255)

                                        cTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcgc                                                                                                              <  2:1051185/149‑1 (MQ=255)

                                         ttCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcgcg                                                                                                             >  2:2832123/1‑149 (MQ=255)

                                               gATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaa                                                                                                       >  2:4335571/1‑149 (MQ=255)

                                                attattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCCAATAAACCATTCAAAAAACGTTCAAGCGCAACACGCGAACTaaa                                                                                                      >  1:969292/1‑149 (MQ=255)

                                                attattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa                                                                                                      >  1:2566460/1‑149 (MQ=255)

                                                  tattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGc                                                                                                    <  1:2267243/149‑1 (MQ=255)

                                                   attGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGcc                                                                                                   >  1:2020276/1‑149 (MQ=255)

                                                   attGCCGCCGACTGTTTAAAGTAAGCCGTTACCAGCAGGTAAAAGCCTGACACTTCATTTAGCGACTGGCATCGCCAGTCGCGACTTTAACTGGCTTAGCAAAATAAACCATTCAAATAACTTAAAATAGCAACACGCGAACAAAAGca                                                                                                   >  2:1723429/1‑148 (MQ=255)

                                                    ttGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCa                                                                                                  <  2:3058868/149‑1 (MQ=255)

                                                           cgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                                           <  1:4038074/149‑1 (MQ=255)

                                                           cgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                                           <  1:2177853/149‑1 (MQ=255)

                                                           cgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGa                                                                                           <  1:4741781/149‑1 (MQ=255)

                                                             aCTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGGCGCGTCTTTAACTGGCTTCGCCCAATAAACCATTCAAATAACGTTCAAGCGCAAAACGCGAACAAAAGCCATGCGGGATa                                                                                         >  1:3675387/1‑149 (MQ=255)

                                                                  ttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTa                                                                                    <  1:2360028/149‑1 (MQ=255)

                                                                   tAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTaa                                                                                   <  1:290113/149‑1 (MQ=255)

                                                                   tAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTaa                                                                                   <  1:2799024/149‑1 (MQ=255)

                                                                   gaaaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTGAAGCCATGCGGGATACCGTaa                                                                                   <  1:2798923/148‑1 (MQ=255)

                                                                    aaaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt                                                                                  >  2:3872787/1‑149 (MQ=255)

                                                                    aaaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt                                                                                  <  2:1900615/149‑1 (MQ=255)

                                                                    aaaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt                                                                                  <  2:86385/149‑1 (MQ=255)

                                                                     aaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATg                                                                                 >  1:4112273/1‑149 (MQ=255)

                                                                       gTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCt                                                                               >  2:5202675/1‑149 (MQ=255)

                                                                        tCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTc                                                                              <  1:3729104/149‑1 (MQ=255)

                                                                        tCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTc                                                                              >  2:4246881/1‑149 (MQ=255)

                                                                         cAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCg                                                                             >  2:1571131/1‑149 (MQ=255)

                                                                           gCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCgtg                                                                           <  2:2206530/149‑1 (MQ=255)

                                                                            ccGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCgtgt                                                                          <  2:741572/149‑1 (MQ=255)

                                                                               ttACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgc                                                                       <  2:1290060/149‑1 (MQ=255)

                                                                                tACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgct                                                                      <  1:4949664/149‑1 (MQ=255)

                                                                                tACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACGGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgct                                                                      <  1:4187391/149‑1 (MQ=255)

                                                                                   cagcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgctcgc                                                                   >  2:2793977/1‑149 (MQ=255)

                                                                                    agcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGACCTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGcc                                                                  >  2:1093113/1‑149 (MQ=255)

                                                                                    agcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTCAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGcc                                                                  >  2:3250898/1‑149 (MQ=255)

                                                                                       agGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGc                                                               <  2:1833623/149‑1 (MQ=255)

                                                                                            aaaGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCCAATAAAACATTCAAATAAAGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGGGTCGCTCGCCTGCTaaaa                                                          >  1:3939443/1‑148 (MQ=255)

                                                                                               gCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATaga                                                       <  2:2189636/149‑1 (MQ=255)

                                                                                               gCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATaga                                                       >  1:2940853/1‑149 (MQ=255)

                                                                                               gCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAACATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAAGCTCGTGTCGCTCGCCTGCTAAATaga                                                       >  1:2068844/1‑149 (MQ=255)

                                                                                               gCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTGAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCACCACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATaga                                                       <  2:3234217/149‑1 (MQ=255)

                                                                                                ccTGACTCTTCTTTTCGCGACTGGCATAGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATagag                                                      >  1:724021/1‑149 (MQ=255)

                                                                                                  tGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAATCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCg                                                    >  1:2478931/1‑149 (MQ=255)

                                                                                                     ctctTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGaa                                                 >  1:789215/1‑149 (MQ=255)

                                                                                                         tcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTTGCTTCGCCAAATAAAACAATCAAATAACGTTAAAGCGCAACACCCGAACTAAAGCCATGCGGGATACCGTAATTCTAGTGTCGCTCGCCAGCTAAATAGAGCGGAAAttt                                             >  1:1779531/1‑148 (MQ=255)

                                                                                                         tcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTg                                             >  1:4595999/1‑149 (MQ=255)

                                                                                                         tcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAACATTCAAAAAACGTTCAATCGCAACAAGCCAACAAAAGCCAACCGGGATAACGTAATTCTAGTGTCGCTAGCCTGCTAAATATAGCGGAAAttt                                             >  1:3165093/1‑148 (MQ=255)

                                                                                                           ttttCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCt                                           <  1:3696074/149‑1 (MQ=255)

                                                                                                              tCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGt                                        <  2:3551239/149‑1 (MQ=255)

                                                                                                                 cgACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAAt                                     <  1:1592447/149‑1 (MQ=255)

                                                                                                                 cgACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAAt                                     <  2:4604864/149‑1 (MQ=255)

                                                                                                                 cgACGGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAAt                                     <  2:3136287/149‑1 (MQ=255)

                                                                                                                  gACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCTAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATg                                    >  2:1278124/1‑149 (MQ=255)

                                                                                                                  gACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATg                                    >  2:3300084/1‑149 (MQ=255)

                                                                                                                   aCTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGa                                   <  2:4701694/149‑1 (MQ=255)

                                                                                                                       gCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGc                               >  2:3414750/1‑149 (MQ=255)

                                                                                                                         aTCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa                             <  2:913252/149‑1 (MQ=255)

                                                                                                                          tCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAg                            >  1:1237540/1‑149 (MQ=255)

                                                                                                                            gCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGtt                          <  1:386640/149‑1 (MQ=255)

                                                                                                                             ccAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGttt                         <  2:3090152/149‑1 (MQ=255)

                                                                                                                                 tCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAat                     <  1:4431633/149‑1 (MQ=255)

                                                                                                                                  cgcgTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata                    <  2:3960396/149‑1 (MQ=255)

                                                                                                                                  cgcgTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata                    <  1:2472647/149‑1 (MQ=255)

                                                                                                                                   gcgTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAatat                   <  1:4500269/149‑1 (MQ=255)

                                                                                                                                      tCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCgg                >  2:765602/1‑149 (MQ=255)

                                                                                                                                        tttAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGaa              >  2:4207725/1‑149 (MQ=255)

                                                                                                                                        tttAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGaa              >  2:3776340/1‑149 (MQ=255)

                                                                                                                                        tttAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGaa              <  2:4930854/149‑1 (MQ=255)

                                                                                                                                         ttAACTGGCTTCGCCTAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAg             <  1:557337/149‑1 (MQ=255)

                                                                                                                                            aCTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCgg          <  1:2223866/149‑1 (MQ=255)

                                                                                                                                                gCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTc      >  1:1555023/1‑149 (MQ=255)

                                                                                                                                                  ttCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAg    >  1:5174593/1‑149 (MQ=255)

ACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCC  >  CP009273/2792872‑2793168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`