Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,820,083 C→A 7.1% G111G (GGC→GGA)  srlE → glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,820,0830CA 7.1% 1324.7 / 55.8 346G111G (GGC→GGA) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base C (148/173);  new base A (10/15);  total (158/188)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 346 total aligned reads displayed.

GGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCG  >  CP009273/2819936‑2820230
                                                                                                                                                   |                                                                                                                                                   
gtaaaTTGGTGTCGGGTTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCg                                                                                                                                                    <  1:4011707/147‑1 (MQ=255)
   aaTTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTaa                                                                                                                                                 >  1:791515/1‑149 (MQ=255)
     ttGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTaaaa                                                                                                                                               <  2:2474307/149‑1 (MQ=255)
     ttGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTaaaa                                                                                                                                               <  1:4387896/149‑1 (MQ=255)
       ggTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAaaga                                                                                                                                             <  1:3823853/149‑1 (MQ=255)
         tgtCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGAGTAAaagaag                                                                                                                                           <  1:3784273/149‑1 (MQ=255)
          gtCGCGTTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAagaaga                                                                                                                                          <  1:1826148/149‑1 (MQ=255)
          gtCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAagaaga                                                                                                                                          <  1:4421118/149‑1 (MQ=255)
           tCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCTTCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAgaagaa                                                                                                                                         <  2:3889988/149‑1 (MQ=255)
               ggTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACa                                                                                                                                     <  1:4836207/149‑1 (MQ=255)
               ggTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACa                                                                                                                                     <  1:3347079/149‑1 (MQ=255)
                 tAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATc                                                                                                                                   <  2:294142/149‑1 (MQ=255)
                  aaTCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCa                                                                                                                                  >  2:1765189/1‑149 (MQ=255)
                   aTCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCAc                                                                                                                                 <  2:1844633/149‑1 (MQ=255)
                   aTCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCAc                                                                                                                                 >  1:183380/1‑149 (MQ=255)
                    tCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACt                                                                                                                                >  2:4853800/1‑149 (MQ=255)
                      gACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTgt                                                                                                                              >  1:2740278/1‑149 (MQ=255)
                      gACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGAAGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTgt                                                                                                                              <  2:2859208/149‑1 (MQ=255)
                        cTGTGGCGGCACATTACGCTGCGGCATCTATCCGACACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTgtag                                                                                                                            <  1:466122/149‑1 (MQ=255)
                        cTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTgtag                                                                                                                            <  2:1617522/149‑1 (MQ=255)
                             gcggcACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGt                                                                                                                       <  2:433594/149‑1 (MQ=255)
                             gcggcACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGt                                                                                                                       <  1:3754935/149‑1 (MQ=255)
                                  acaTTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGc                                                                                                                  >  1:1178395/1‑149 (MQ=255)
                                  acaTTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGc                                                                                                                  >  1:2260014/1‑149 (MQ=255)
                                     ttACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGac                                                                                                               >  2:2343246/1‑149 (MQ=255)
                                     ttACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGac                                                                                                               >  1:361458/1‑149 (MQ=255)
                                      tACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGaca                                                                                                              <  1:1598550/149‑1 (MQ=255)
                                      tACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGAGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGaca                                                                                                              <  2:3871343/149‑1 (MQ=255)
                                        cGCTGCGGAATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGAcacc                                                                                                            <  1:2627514/149‑1 (MQ=255)
                                         gCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGAcacca                                                                                                           <  2:4251276/149‑1 (MQ=255)
                                            gCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACaa                                                                                                        >  1:4696107/1‑149 (MQ=255)
                                                  tCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCctct                                                                                                  >  1:1678104/1‑149 (MQ=255)
                                                   cTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCtt                                                                                                 <  2:4616226/149‑1 (MQ=255)
                                                    tATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                                <  2:4687580/149‑1 (MQ=255)
                                                     aTCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTcc                                                                                               >  1:2939893/1‑149 (MQ=255)
                                                         gAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                                           >  2:856471/1‑149 (MQ=255)
                                                         gAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                                           <  1:2465019/149‑1 (MQ=255)
                                                          aaaCGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                          >  2:2715060/1‑149 (MQ=255)
                                                           aaCGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                         <  2:369664/149‑1 (MQ=255)
                                                           aaCGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGAGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                         >  1:1720465/1‑149 (MQ=255)
                                                            acgacgTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                                        <  2:2384641/149‑1 (MQ=255)
                                                                 gTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                                   <  1:2569191/149‑1 (MQ=255)
                                                                   aTTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                                 <  1:4133138/149‑1 (MQ=255)
                                                                    ttCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                                >  2:562899/1‑149 (MQ=255)
                                                                    ttCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                                <  1:866840/149‑1 (MQ=255)
                                                                          ccaTTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                          <  1:2808859/149‑1 (MQ=255)
                                                                             ttAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                                       <  1:1320540/149‑1 (MQ=255)
                                                                             ttAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                                       <  1:3916991/149‑1 (MQ=255)
                                                                             ttAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACAGTGTGGAAGATATTTATGTCTCTGGAGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                                       >  2:1760481/1‑149 (MQ=255)
                                                                               aaTATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                                                     <  2:2115085/149‑1 (MQ=255)
                                                                                 tatCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                                   >  2:3192730/1‑149 (MQ=255)
                                                                                 tatCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                                   <  2:1683440/149‑1 (MQ=255)
                                                                                 tatCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                                   >  2:3193372/1‑149 (MQ=255)
                                                                                      aCTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                              >  2:40837/1‑149 (MQ=255)
                                                                                      aCTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                              >  2:1364808/1‑149 (MQ=255)
                                                                                       cTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGACGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                             >  2:4435647/1‑149 (MQ=255)
                                                                                       cTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                             >  1:3728794/1‑149 (MQ=255)
                                                                                       cTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                             <  2:700179/149‑1 (MQ=255)
                                                                                       cTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                             <  2:773005/149‑1 (MQ=255)
                                                                                        tCGACGGTCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                            <  2:4109735/149‑1 (MQ=255)
                                                                                        tCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                            <  2:2003209/149‑1 (MQ=255)
                                                                                         cGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcc                                                           <  2:4282606/149‑1 (MQ=255)
                                                                                              ggCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                                      >  2:311216/1‑149 (MQ=255)
                                                                                               gCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                                     <  2:4154111/149‑1 (MQ=255)
                                                                                                cAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                                    <  2:3545034/149‑1 (MQ=255)
                                                                                                 aaGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                                   <  1:3088434/149‑1 (MQ=255)
                                                                                                  aGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                                  >  1:954475/1‑149 (MQ=255)
                                                                                                  aGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                                  >  1:3967149/1‑149 (MQ=255)
                                                                                                    tccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                                <  2:129068/149‑1 (MQ=255)
                                                                                                    tccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                                >  2:1696136/1‑149 (MQ=255)
                                                                                                     ccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                               <  1:1265643/149‑1 (MQ=255)
                                                                                                     ccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                               <  2:5179567/149‑1 (MQ=255)
                                                                                                      cggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                                              <  1:4147904/149‑1 (MQ=255)
                                                                                                       ggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                             >  1:1008834/1‑149 (MQ=255)
                                                                                                         tccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                                           <  2:1455881/149‑1 (MQ=255)
                                                                                                          ccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt                                          <  1:1077816/149‑1 (MQ=255)
                                                                                                            gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                        <  2:154523/149‑1 (MQ=255)
                                                                                                              tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGAGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                                      <  1:2887736/149‑1 (MQ=255)
                                                                                                               ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                                     >  2:4549315/1‑149 (MQ=255)
                                                                                                                      tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt                              <  1:595609/149‑1 (MQ=255)
                                                                                                                        cATTGTTGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                            <  2:2683172/149‑1 (MQ=255)
                                                                                                                        cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                            >  2:1898829/1‑149 (MQ=255)
                                                                                                                          ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGa                          >  1:1796518/1‑149 (MQ=255)
                                                                                                                           tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                         <  1:1514041/149‑1 (MQ=255)
                                                                                                                           tgtgGAAGATATTTATGTCTCTGGAGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                         <  2:245289/149‑1 (MQ=255)
                                                                                                                            gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAt                        >  1:216286/1‑149 (MQ=255)
                                                                                                                             tgGAAGATATTTATGTCTCTGGAGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg                       >  2:3765155/1‑149 (MQ=255)
                                                                                                                               gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg                     >  2:3421553/1‑149 (MQ=255)
                                                                                                                                aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc                    <  2:22127/149‑1 (MQ=255)
                                                                                                                                  gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt                  >  1:2023527/1‑149 (MQ=255)
                                                                                                                                   atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg                 >  2:5129388/1‑149 (MQ=255)
                                                                                                                                    tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgg                <  1:4041653/149‑1 (MQ=255)
                                                                                                                                    tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgg                <  1:352192/149‑1 (MQ=255)
                                                                                                                                     atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg               >  2:1151565/1‑149 (MQ=255)
                                                                                                                                         aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGACTGGGCATGGGGTcc           >  2:1222640/1‑149 (MQ=255)
                                                                                                                                         aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTcc           <  1:4338221/149‑1 (MQ=255)
                                                                                                                                             ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCCTGGGGTCCACCg       >  2:2087225/1‑149 (MQ=255)
                                                                                                                                              tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCCTGGGGTCCACCGt      >  2:2551778/1‑149 (MQ=255)
                                                                                                                                              tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGt      >  1:3381656/1‑149 (MQ=255)
                                                                                                                                                  gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCg  >  1:501826/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGAAATTGGTGTCGCGGTAATCGACTGTGGCGGCACATTACGCTGCGGCATCTATCCGAAACGACGTATTCCCACCATTAATATCCACTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCG  >  CP009273/2819936‑2820230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑