Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,971,037 G→A 5.5% R408C (CGC→TGC)  lysA ← diaminopimelate decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,971,0370GA 5.5% 1193.3 / 36.6 325R408C (CGC→TGC) lysAdiaminopimelate decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base G (153/154);  new base A (12/6);  total (165/160)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 327 total aligned reads displayed.

CTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGG  >  CP009273/2970889‑2971185
                                                                                                                                                    |                                                                                                                                                    
  ctcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCAAc                                                                                                                                                    >  1:2393424/1‑149 (MQ=255)
   tcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACg                                                                                                                                                   >  1:4632181/1‑149 (MQ=255)
   tcCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACg                                                                                                                                                   >  1:3953040/1‑149 (MQ=255)
       ggAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGa                                                                                                                                               <  2:3338224/149‑1 (MQ=255)
       ggAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCAACGGCGa                                                                                                                                               <  1:444306/149‑1 (MQ=255)
         aaaTCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAAt                                                                                                                                             >  1:4004115/1‑149 (MQ=255)
             cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAAc                                                                                                                                         <  2:4951198/149‑1 (MQ=255)
             cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAAc                                                                                                                                         <  2:2373222/149‑1 (MQ=255)
             cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAAc                                                                                                                                         >  1:2170284/1‑149 (MQ=255)
             cACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCAGTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAAc                                                                                                                                         <  2:4976723/149‑1 (MQ=255)
                tAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACcgc                                                                                                                                      >  1:134158/1‑149 (MQ=255)
                   tGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGcc                                                                                                                                   <  1:206126/149‑1 (MQ=255)
                      cTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                                                                                                                                >  1:4308686/1‑149 (MQ=255)
                      cTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGa                                                                                                                                <  2:3510639/149‑1 (MQ=255)
                       tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAc                                                                                                                               <  1:4790156/149‑1 (MQ=255)
                       tttAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAc                                                                                                                               >  2:2078945/1‑149 (MQ=255)
                         tAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCa                                                                                                                             <  2:407640/149‑1 (MQ=255)
                          tgTCCGCCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGACAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCAt                                                                                                                            <  2:3711823/148‑1 (MQ=255)
                          aGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCAt                                                                                                                            >  2:2011414/1‑149 (MQ=255)
                           gTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCAtt                                                                                                                           >  1:2422670/1‑149 (MQ=255)
                            tCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTa                                                                                                                          <  1:409316/149‑1 (MQ=255)
                              cGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATc                                                                                                                        >  1:1461350/1‑149 (MQ=255)
                                    tGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAg                                                                                                                  >  1:5054297/1‑149 (MQ=255)
                                       tACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAAc                                                                                                               <  1:1204778/149‑1 (MQ=255)
                                          gtcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTc                                                                                                            <  2:3158839/149‑1 (MQ=255)
                                          gtcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTc                                                                                                            <  2:2437475/149‑1 (MQ=255)
                                           tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCt                                                                                                           <  1:1369694/149‑1 (MQ=255)
                                           tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCt                                                                                                           >  1:4339070/1‑149 (MQ=255)
                                             gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAACGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTgg                                                                                                         <  2:3398662/149‑1 (MQ=255)
                                             gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTgg                                                                                                         <  1:4223643/149‑1 (MQ=255)
                                             gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTgg                                                                                                         >  1:4700664/1‑149 (MQ=255)
                                               cATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCTATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTa                                                                                                       <  2:3825296/149‑1 (MQ=255)
                                                aTTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTaa                                                                                                      >  2:2989687/1‑149 (MQ=255)
                                                 ttCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAAc                                                                                                     >  1:535364/1‑149 (MQ=255)
                                                 ttCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAAc                                                                                                     >  1:2066119/1‑149 (MQ=255)
                                                   cAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAg                                                                                                   >  2:1756422/1‑149 (MQ=255)
                                                    aGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                                                                                                  <  2:4049178/149‑1 (MQ=255)
                                                    aGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                                                                                                  <  2:1286238/149‑1 (MQ=255)
                                                    aGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                                                                                                  <  2:1709272/149‑1 (MQ=255)
                                                    aGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCAACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGc                                                                                                  <  2:224136/149‑1 (MQ=255)
                                                       gtCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGa                                                                                               <  1:805947/149‑1 (MQ=255)
                                                        tCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGAc                                                                                              <  2:2858933/149‑1 (MQ=255)
                                                          aaCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACgg                                                                                            >  2:2925995/1‑149 (MQ=255)
                                                             tCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTa                                                                                         <  2:1545416/149‑1 (MQ=255)
                                                              ccGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTAt                                                                                        <  1:3133806/149‑1 (MQ=255)
                                                                 tCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGt                                                                                     >  2:5183390/1‑149 (MQ=255)
                                                                   gCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAg                                                                                   <  2:4168776/149‑1 (MQ=255)
                                                                   gCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAg                                                                                   <  2:1180532/149‑1 (MQ=255)
                                                                         ggCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGAt                                                                             >  1:3629055/1‑149 (MQ=255)
                                                                         ggCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGAt                                                                             <  2:2708365/149‑1 (MQ=255)
                                                                              gTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACAt                                                                        <  1:4851614/149‑1 (MQ=255)
                                                                              gTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACAt                                                                        <  2:2628734/149‑1 (MQ=255)
                                                                              gTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACAt                                                                        <  2:2735142/149‑1 (MQ=255)
                                                                              gTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACAt                                                                        <  2:2119983/149‑1 (MQ=255)
                                                                                 atcatGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCTATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGCCGGCTATTGTAGTGGGATGACATTGa                                                                     >  2:2492187/1‑149 (MQ=255)
                                                                                 atcatGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGa                                                                     <  2:1502830/149‑1 (MQ=255)
                                                                                     tGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATgcg                                                                 >  1:1550646/1‑149 (MQ=255)
                                                                                      gCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATgcgc                                                                <  2:3073785/149‑1 (MQ=255)
                                                                                      gCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATgcgc                                                                <  2:20596/149‑1 (MQ=255)
                                                                                       cAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGAGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGcc                                                               <  2:3243092/149‑1 (MQ=255)
                                                                                        aaCCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCa                                                              >  1:2264161/1‑149 (MQ=255)
                                                                                           cAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCatat                                                           <  1:1024309/149‑1 (MQ=255)
                                                                                           cAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCatat                                                           <  2:1846333/149‑1 (MQ=255)
                                                                                           cAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCAACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCatat                                                           >  1:1714207/1‑149 (MQ=255)
                                                                                            aGCGACTAACCGCAGTTAACGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATg                                                          <  1:2797344/149‑1 (MQ=255)
                                                                                             gCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGc                                                         >  1:5011330/1‑149 (MQ=255)
                                                                                                 cTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCt                                                     >  1:3218445/1‑149 (MQ=255)
                                                                                                   aaCCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGt                                                   >  1:936494/1‑149 (MQ=255)
                                                                                                   aaCCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGt                                                   >  1:767997/1‑149 (MQ=255)
                                                                                                    aCCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTa                                                  <  1:1820583/149‑1 (MQ=255)
                                                                                                    aCCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTa                                                  <  1:5183390/149‑1 (MQ=255)
                                                                                                        cAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCAt                                              <  2:4678656/149‑1 (MQ=255)
                                                                                                         aGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATg                                             <  2:779598/149‑1 (MQ=255)
                                                                                                         aGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATg                                             <  2:2412712/149‑1 (MQ=255)
                                                                                                         aGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCAACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGCCGGCTATTGTAGTTGGATGACATTGATGCGCCCTATGACCCTGTCTCATg                                             >  2:3918593/1‑149 (MQ=255)
                                                                                                          gTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGc                                            <  1:1957859/149‑1 (MQ=255)
                                                                                                          gTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGc                                            >  1:2597683/1‑149 (MQ=255)
                                                                                                           ttAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCa                                           <  1:2566778/149‑1 (MQ=255)
                                                                                                             aaaGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGt                                         >  2:590450/1‑149 (MQ=255)
                                                                                                               aGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCACATGCCCCTGTATCATGCAGTAc                                       >  1:3710886/1‑149 (MQ=255)
                                                                                                                   aTTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGa                                   >  2:3146719/1‑149 (MQ=255)
                                                                                                                   aTTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGa                                   >  2:3033820/1‑149 (MQ=255)
                                                                                                                   aTTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGa                                   <  2:510543/149‑1 (MQ=255)
                                                                                                                      ccAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATaa                                <  2:3377892/149‑1 (MQ=255)
                                                                                                                      ccAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATaa                                <  2:2308827/149‑1 (MQ=255)
                                                                                                                      ccAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATaa                                <  1:1643524/149‑1 (MQ=255)
                                                                                                                        aGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATc                              <  2:1208401/149‑1 (MQ=255)
                                                                                                                         gcgcCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCa                             >  2:1800894/1‑149 (MQ=255)
                                                                                                                            ccAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCt                          >  1:1093059/1‑149 (MQ=255)
                                                                                                                            ccAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCt                          >  1:330241/1‑149 (MQ=255)
                                                                                                                             cAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATAACCTg                         >  2:3633836/1‑149 (MQ=255)
                                                                                                                              aGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGc                        >  1:2991837/1‑149 (MQ=255)
                                                                                                                              aGTAATTCTTCGATGGTCTGGCAACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGc                        <  2:847777/149‑1 (MQ=255)
                                                                                                                                 aaTTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCtt                     <  2:2087594/149‑1 (MQ=255)
                                                                                                                                   ttcttcGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCa                   >  2:5041653/1‑149 (MQ=255)
                                                                                                                                   ttcttcGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCa                   <  2:1369523/149‑1 (MQ=255)
                                                                                                                                    tcttcGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCAc                  <  2:4657940/149‑1 (MQ=255)
                                                                                                                                        cGATGGTCTGGCGACGGCGAATCAACCGCGCCTGAACATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTcc              >  2:154809/1‑149 (MQ=255)
                                                                                                                                               cTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAgg       <  2:4313309/149‑1 (MQ=255)
                                                                                                                                                 ggCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGgcg     >  1:3468770/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTCTCCCGGAAATCACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGCGACGGCGAATCAACCGCGCCTGACCATTATCAAACAGAACTTCTGGTAACAGCGGACGGCTATTGTAGTTGGATGACATTGATGCGCCATATGCCCCTGTATCATGCAGTACCAGATAATCACCTGCCTTCACTTCCGGCAAGGCGCGG  >  CP009273/2970889‑2971185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑