BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,982,873	G→T	10.4%	G71* (GGA→TGA)	yqeJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,982,873	0	G	T	10.4%	957.9 / 63.7	263	G71* (GGA→TGA)	yqeJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (122/113); new base T (10/18); total (132/131)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

ACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTT  >  CP009273/2982726‑2983019

aCTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTgg                                                                                                                                                   >  2:4791335/1‑149 (MQ=255)

  tGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGaa                                                                                                                                                 <  2:599191/149‑1 (MQ=255)

  tGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGaa                                                                                                                                                 >  2:1349339/1‑149 (MQ=255)

  tGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGaa                                                                                                                                                 <  2:2325497/149‑1 (MQ=255)

  tGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGaa                                                                                                                                                 >  2:2921784/1‑149 (MQ=255)

    tCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAAcc                                                                                                                                               <  2:2533836/149‑1 (MQ=255)

      tATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTg                                                                                                                                             <  1:134993/149‑1 (MQ=255)

      tATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTg                                                                                                                                             <  2:4865454/149‑1 (MQ=255)

      tATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTg                                                                                                                                             <  2:2261792/149‑1 (MQ=255)

       aTTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTgg                                                                                                                                            >  1:1200190/1‑149 (MQ=255)

        ttCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGc                                                                                                                                           <  1:3404786/149‑1 (MQ=255)

         tCTTACACTGCAGAAAAAATGATATATAACGAACCATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGca                                                                                                                                          <  1:3815232/149‑1 (MQ=255)

           ttACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGcaac                                                                                                                                        <  2:4368875/149‑1 (MQ=255)

                cTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGt                                                                                                                                   <  1:2701520/149‑1 (MQ=255)

                 tGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTa                                                                                                                                  <  1:1141980/149‑1 (MQ=255)

                  gCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTaa                                                                                                                                 <  2:2984415/149‑1 (MQ=255)

                    aGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACa                                                                                                                               >  2:3102274/1‑149 (MQ=255)

                      aaaaaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACaga                                                                                                                             >  2:4297742/1‑149 (MQ=255)

                       aaaaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACagag                                                                                                                            >  2:3164721/1‑149 (MQ=255)

                        aaaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAgaga                                                                                                                           <  1:898217/149‑1 (MQ=255)

                        aaaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAgaga                                                                                                                           <  1:984241/149‑1 (MQ=255)

                         aaaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGaa                                                                                                                          >  2:788768/1‑149 (MQ=255)

                          aaTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGaaa                                                                                                                         >  1:3387295/1‑149 (MQ=255)

                           aTGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAg                                                                                                                        >  2:3382078/1‑149 (MQ=255)

                             gATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTa                                                                                                                      >  2:4453410/1‑149 (MQ=255)

                                 tataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATc                                                                                                                  <  2:4053317/149‑1 (MQ=255)

                                  ataACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCa                                                                                                                 >  2:769489/1‑149 (MQ=255)

                                   taACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCaa                                                                                                                >  1:4254752/1‑149 (MQ=255)

                                    aaCGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAAt                                                                                                               >  1:5179303/1‑149 (MQ=255)

                                     aCGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATa                                                                                                              >  1:3458605/1‑149 (MQ=255)

                                     aCGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATa                                                                                                              <  1:4268459/149‑1 (MQ=255)

                                      cGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATAc                                                                                                             <  2:4270032/149‑1 (MQ=255)

                                      cGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATAc                                                                                                             <  1:783700/149‑1 (MQ=255)

                                      cGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATAc                                                                                                             <  2:2617510/149‑1 (MQ=255)

                                       gAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACg                                                                                                            <  1:17237/149‑1 (MQ=255)

                                         aaCATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGc                                                                                                          <  1:1633903/149‑1 (MQ=255)

                                         aaCATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑‑AAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCg                                                                                                         >  1:1417948/1‑149 (MQ=255)

                                           cATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGa                                                                                                        >  1:2380166/1‑149 (MQ=255)

                                            aTGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGaa                                                                                                       <  2:365392/149‑1 (MQ=255)

                                             tGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGaaa                                                                                                      >  1:3608413/1‑149 (MQ=255)

                                              gCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAat                                                                                                     >  1:3591062/1‑149 (MQ=255)

                                              gCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAat                                                                                                     <  1:2574551/149‑1 (MQ=255)

                                               cACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAACAAAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAat                                                                                                     <  1:4640986/149‑1 (MQ=255)

                                                aCTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAatat                                                                                                   >  1:4718004/1‑149 (MQ=255)

                                                aCTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAatat                                                                                                   >  1:4718065/1‑149 (MQ=255)

                                                 cTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATAtt                                                                                                  <  2:438733/149‑1 (MQ=255)

                                                 cTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATAtt                                                                                                  <  2:4394996/149‑1 (MQ=255)

                                                     aaaTTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAAt                                                                                              <  1:4784714/149‑1 (MQ=255)

                                                      aaTTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATa                                                                                             >  1:2881773/1‑149 (MQ=255)

                                                      aaTTGGGTAATTTTTAATGACCAGGGACGAGCAAATATTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATa                                                                                             <  2:5023169/149‑1 (MQ=255)

                                                            gTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATg                                                                                       <  2:3837540/149‑1 (MQ=255)

                                                                      aTGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAAc                                                                             >  2:3243586/1‑149 (MQ=255)

                                                                         aCCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTAt                                                                          <  1:572509/149‑1 (MQ=255)

                                                                          ccAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑‑AAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGa                                                                        <  1:3315284/149‑1 (MQ=255)

                                                                            aGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGac                                                                       <  2:1297541/149‑1 (MQ=255)

                                                                                    gCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCa                                                               <  1:2975493/149‑1 (MQ=255)

                                                                                     cAAATCTGACCCTCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAACTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCAt                                                              <  2:4901837/149‑1 (MQ=255)

                                                                                        aTCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTa                                                           >  1:481977/1‑149 (MQ=255)

                                                                                         tCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTaa                                                          >  2:1039903/1‑149 (MQ=255)

                                                                                           ttACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACa                                                        <  2:3633500/149‑1 (MQ=255)

                                                                                            tACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACAt                                                       >  1:3294005/1‑149 (MQ=255)

                                                                                                aTCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaa                                                   >  2:4735645/1‑149 (MQ=255)

                                                                                                  cGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaaga                                                 >  2:1924299/1‑149 (MQ=255)

                                                                                                     cTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaagaaag                                              >  2:2210185/1‑149 (MQ=255)

                                                                                                     cTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaagaaag                                              <  1:2784187/149‑1 (MQ=255)

                                                                                                     cTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCaaagaaag                                              >  1:818004/1‑149 (MQ=255)

                                                                                                      tttATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGt                                             >  1:2177701/1‑149 (MQ=255)

                                                                                                         aTGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaa                                          <  2:2583438/149‑1 (MQ=255)

                                                                                                          tGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaat                                         >  1:1519733/1‑149 (MQ=255)

                                                                                                            tataAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaataa                                       <  2:4707980/149‑1 (MQ=255)

                                                                                                            tataAA‑AAAAAAAATAAAAAAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTtaataa                                       <  2:128574/149‑1 (MQ=255)

                                                                                                               aaC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGtt                                    >  1:1331196/1‑149 (MQ=255)

                                                                                                                aC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGttt                                   >  1:2371652/1‑149 (MQ=255)

                                                                                                                 c‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTg                                  <  2:339211/149‑1 (MQ=255)

                                                                                                                 c‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTg                                  <  1:5165212/149‑1 (MQ=255)

                                                                                                                     aaaaaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTgaga                               >  2:2046200/1‑149 (MQ=255)

                                                                                                                        aaaTAAAACAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTa                            >  1:1740536/1‑149 (MQ=255)

                                                                                                                        aaaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTa                            <  1:4507842/149‑1 (MQ=255)

                                                                                                                         aaTAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTAt                           >  2:272758/1‑149 (MQ=255)

                                                                                                                           tAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATgg                         <  1:261843/149‑1 (MQ=255)

                                                                                                                            aaaaCAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGa                        >  2:164704/1‑149 (MQ=255)

                                                                                                                            aaaaCAGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGa                        >  2:1768602/1‑149 (MQ=255)

                                                                                                                              aaCAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATc                      >  1:720369/1‑149 (MQ=255)

                                                                                                                               aCAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCa                     >  1:3201171/1‑149 (MQ=255)

                                                                                                                                 aGGAACTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAg                   >  2:3432693/1‑149 (MQ=255)

                                                                                                                                 aGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAg                   >  2:2229217/1‑149 (MQ=255)

                                                                                                                                    aaCTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtg                <  1:3164721/149‑1 (MQ=255)

                                                                                                                                      cTGTTGCGTTGAGTTGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgat              >  2:5168662/1‑149 (MQ=255)

                                                                                                                                       tGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatg             >  1:2931518/1‑149 (MQ=255)

                                                                                                                                       tGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatg             <  2:3919397/149‑1 (MQ=255)

                                                                                                                                       tGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatg             >  1:90697/1‑149 (MQ=255)

                                                                                                                                        gTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatga            >  2:919133/1‑149 (MQ=255)

                                                                                                                                        gTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATAGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgtcat            >  2:3376806/1‑145 (MQ=255)

                                                                                                                                         ttGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTtgatgat           >  1:2862205/1‑149 (MQ=255)

                                                                                                                                             gTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATa       >  1:1269899/1‑149 (MQ=255)

                                                                                                                                              ttGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTGAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAg      >  1:1867986/1‑149 (MQ=255)

                                                                                                                                              ttGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAg      >  1:2062080/1‑149 (MQ=255)

                                                                                                                                                gAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGAc    >  2:1484778/1‑149 (MQ=255)

                                                                                                                                                  gTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACtt  <  2:5179303/149‑1 (MQ=255)

                                                                                                                                                  gTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACtt  >  1:1260435/1‑149 (MQ=255)

ACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATCTTACCATCGACTTTATGTATAAC‑AAAAAAAATAAAACAGGAACTGTTGCGTTGAGTGGAACCTGGCAACAAGGTAACAGAGAAAGTAAATCAATACGGCGAAATATTGAATACACATGGATTGAAAACTATGACACAGCCCATTTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTT  >  CP009273/2982726‑2983019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`