BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,116,892	C→A	5.5%	L91L (CTG→CTT)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,116,892	0	C	A	5.5%	1278.9 / 38.4	326	L91L (CTG→CTT)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base C (115/193); new base A (6/12); total (121/205)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 332 total aligned reads displayed.

CATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGA  >  CP009273/3116744‑3117040

cATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCAcc                                                                                                                                                      <  1:3079442/149‑1 (MQ=255)

 aTCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCa                                                                                                                                                     <  1:3539877/149‑1 (MQ=255)

  tCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAg                                                                                                                                                    >  1:4905545/1‑149 (MQ=255)

      aCAGTGAGCCCCAGCGAGCGGTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACAAGGTAg                                                                                                                                                <  2:1813392/149‑1 (MQ=255)

        aGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGcc                                                                                                                                              <  2:4086367/149‑1 (MQ=255)

         gTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGccc                                                                                                                                             <  1:750447/149‑1 (MQ=255)

            aGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGt                                                                                                                                          <  1:3104857/149‑1 (MQ=255)

            aGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGt                                                                                                                                          >  1:277170/1‑149 (MQ=255)

            aGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGt                                                                                                                                          <  1:2022520/149‑1 (MQ=255)

             gCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGtt                                                                                                                                         <  1:1020321/149‑1 (MQ=255)

             gCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGtt                                                                                                                                         <  2:2208197/149‑1 (MQ=255)

             gCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGtt                                                                                                                                         >  1:2089216/1‑149 (MQ=255)

                  agcgagcgTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGc                                                                                                                                    >  1:984184/1‑149 (MQ=255)

                   gcgagcgTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCa                                                                                                                                   <  2:3389760/149‑1 (MQ=255)

                   gcgagcgTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCa                                                                                                                                   >  2:3338658/1‑149 (MQ=255)

                           tCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGAt                                                                                                                           <  1:1741699/149‑1 (MQ=255)

                           tCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGAt                                                                                                                           <  1:2992357/149‑1 (MQ=255)

                              ccgcGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTta                                                                                                                        <  1:2147316/149‑1 (MQ=255)

                              ccgcGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTta                                                                                                                        <  2:836329/149‑1 (MQ=255)

                                gcgTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTtata                                                                                                                      >  1:1468335/1‑149 (MQ=255)

                                gcgTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTtata                                                                                                                      <  1:1272023/149‑1 (MQ=255)

                                   ttCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCACTGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAACGATTTATAGGc                                                                                                                   >  2:3765554/1‑149 (MQ=255)

                                   ttCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGc                                                                                                                   <  2:4665081/149‑1 (MQ=255)

                                      aGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGc                                                                                                                >  2:2048277/1‑149 (MQ=255)

                                       gcgcGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATATGCCGCt                                                                                                               >  2:2883477/1‑149 (MQ=255)

                                          cgTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTa                                                                                                            <  1:2687454/149‑1 (MQ=255)

                                           gTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTAt                                                                                                           <  2:3282950/149‑1 (MQ=255)

                                           gTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACAAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTAt                                                                                                           <  2:2366330/149‑1 (MQ=255)

                                            tAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATc                                                                                                          <  1:1634663/149‑1 (MQ=255)

                                             aGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCt                                                                                                         <  2:416198/149‑1 (MQ=255)

                                              gCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCtt                                                                                                        <  2:2308113/149‑1 (MQ=255)

                                              gCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCtt                                                                                                        <  1:857741/149‑1 (MQ=255)

                                                 ggCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTa                                                                                                     <  1:4896698/149‑1 (MQ=255)

                                                 ggCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTa                                                                                                     >  1:4592328/1‑149 (MQ=255)

                                                     ttCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCgg                                                                                                 >  2:2801731/1‑149 (MQ=255)

                                                        aTTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGACAGATTTATAGGCCGCTTTATCTTTTACCGGccc                                                                                              >  2:2762551/1‑149 (MQ=255)

                                                         ttGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGTTTGCGGCACAAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCGGCTTTATCTTTTACCGGcccc                                                                                             <  1:3847068/149‑1 (MQ=255)

                                                         ttGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGcccc                                                                                             <  1:3663259/149‑1 (MQ=255)

                                                         ttGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGcccc                                                                                             <  1:4845358/149‑1 (MQ=255)

                                                         ttGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACAAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGcccc                                                                                             <  1:3798019/149‑1 (MQ=255)

                                                           gCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGGCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACAAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTGTTACCGGCCCCgg                                                                                           >  1:1098533/1‑149 (MQ=255)

                                                             cGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGAt                                                                                         <  2:2943166/149‑1 (MQ=255)

                                                             cGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGAt                                                                                         <  2:1594443/149‑1 (MQ=255)

                                                              ggAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGAtt                                                                                        <  2:489024/149‑1 (MQ=255)

                                                                 accaccAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATtgct                                                                                     <  2:1483298/149‑1 (MQ=255)

                                                                  ccaccaGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATtgctg                                                                                    <  1:94253/149‑1 (MQ=255)

                                                                     ccaGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGa                                                                                 <  2:3708349/149‑1 (MQ=255)

                                                                      caGCTGCGGCCCCGCCTGGCTGGCGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGAt                                                                                <  1:1134437/149‑1 (MQ=255)

                                                                          tGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCa                                                                            >  2:1650792/1‑149 (MQ=255)

                                                                           gCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCAc                                                                           >  2:123508/1‑149 (MQ=255)

                                                                                cccGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACtcatc                                                                      <  2:4520117/149‑1 (MQ=255)

                                                                                      tggctggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAgcgc                                                                <  1:2569170/149‑1 (MQ=255)

                                                                                      tggctggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAgcgc                                                                <  1:3219365/149‑1 (MQ=255)

                                                                                      tggctggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAgcgc                                                                <  2:2057717/149‑1 (MQ=255)

                                                                                       ggctggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCt                                                               <  2:2413961/149‑1 (MQ=255)

                                                                                          tggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGcc                                                            <  1:4016975/149‑1 (MQ=255)

                                                                                           ggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCt                                                           >  1:1525324/1‑149 (MQ=255)

                                                                                           ggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCt                                                           >  1:4744426/1‑149 (MQ=255)

                                                                                           ggTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCt                                                           <  1:3330788/149‑1 (MQ=255)

                                                                                               aTTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAAt                                                       >  1:1900196/1‑149 (MQ=255)

                                                                                                 ttCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGc                                                     <  1:4248871/149‑1 (MQ=255)

                                                                                                 ttCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGc                                                     <  2:4430048/149‑1 (MQ=255)

                                                                                                     cTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATc                                                 >  1:451966/1‑149 (MQ=255)

                                                                                                     cTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATc                                                 >  1:4379167/1‑149 (MQ=255)

                                                                                                      tGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATcg                                                >  1:4730045/1‑149 (MQ=255)

                                                                                                      tGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATcg                                                >  1:4662508/1‑149 (MQ=255)

                                                                                                       gTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGAGTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATcgc                                               >  2:3612877/1‑149 (MQ=255)

                                                                                                          aaTGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGtt                                            >  2:298886/1‑149 (MQ=255)

                                                                                                          aaTGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGtt                                            <  1:1366834/149‑1 (MQ=255)

                                                                                                           aTGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTc                                           <  1:5066900/149‑1 (MQ=255)

                                                                                                           aTGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTc                                           >  2:1119969/1‑149 (MQ=255)

                                                                                                              cccGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGc                                        <  1:5028076/149‑1 (MQ=255)

                                                                                                                 gTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAg                                     >  1:4688781/1‑149 (MQ=255)

                                                                                                                  tGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGc                                    <  1:5171790/149‑1 (MQ=255)

                                                                                                                       tCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTg                               <  2:3949552/149‑1 (MQ=255)

                                                                                                                        ccACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGa                              <  2:5043916/149‑1 (MQ=255)

                                                                                                                        ccACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGa                              <  2:4767246/149‑1 (MQ=255)

                                                                                                                        ccACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGa                              >  2:3142835/1‑149 (MQ=255)

                                                                                                                        ccACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGa                              >  1:2279876/1‑149 (MQ=255)

                                                                                                                         cACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGAc                             <  1:4721272/149‑1 (MQ=255)

                                                                                                                           ccGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGa                           <  2:595464/149‑1 (MQ=255)

                                                                                                                           ccGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGa                           <  2:724369/149‑1 (MQ=255)

                                                                                                                           ccGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATCTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGa                           <  1:4332399/149‑1 (MQ=255)

                                                                                                                            cGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGAt                          <  1:559170/149‑1 (MQ=255)

                                                                                                                             gTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGAtt                         >  2:2822368/1‑149 (MQ=255)

                                                                                                                             gTCACGCGCTCCGGTTGCGGCACAAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGAtt                         <  1:2065098/149‑1 (MQ=255)

                                                                                                                              tCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGAttt                        <  1:3673129/149‑1 (MQ=255)

                                                                                                                               cACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGAtttt                       >  1:4475073/1‑149 (MQ=255)

                                                                                                                               cACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGAtttt                       >  2:2319648/1‑149 (MQ=255)

                                                                                                                                aCGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTc                      <  1:2417317/149‑1 (MQ=255)

                                                                                                                                aCGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGAGTTCTGCCAGCAACTGACGATTTTc                      <  1:2597028/149‑1 (MQ=255)

                                                                                                                                aCGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCAATCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTc                      <  2:2490201/149‑1 (MQ=255)

                                                                                                                                 cgcgCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCt                     <  2:4315288/149‑1 (MQ=255)

                                                                                                                                  gcgcTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTg                    >  2:4992006/1‑149 (MQ=255)

                                                                                                                                       ccGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCAATCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGcc               <  1:2139655/149‑1 (MQ=255)

                                                                                                                                        cGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCa              <  1:2517397/149‑1 (MQ=255)

                                                                                                                                          ggtGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGa            <  1:3243389/147‑1 (MQ=255)

                                                                                                                                            tGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATc          <  1:2355140/149‑1 (MQ=255)

                                                                                                                                             gCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCa         <  1:878121/149‑1 (MQ=255)

                                                                                                                                                 cACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGaa     <  2:4730045/149‑1 (MQ=255)

CATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTCAGCGCGTAGCGGGCGTTCATTGCCGGAACCACCAGCTGCGGCCCCGCCTGGCTGGTGATTTCGCTGTCAATGCCCGTGGTTTCCACCGTCACGCGCTCCGGTTGCGGCACCAGGTAGCCCAGTTCACGCAGGAAAGATTTATAGGCCGCTTTATCTTTTACCGGCCCCGGATTGCTGCGATGCCACTCATCAAGCGCTGCCTGAATGCGATCGCGTTCTGCCAGCAACTGACGATTTTCTGGTGCCAGATCATGAACGA  >  CP009273/3116744‑3117040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`