Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,202,506 C→A 5.3% S375* (TCG→TAG)  ttdT → L‑tartrate/succinate antiporte

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,202,5060CA 5.3% 1135.4 / 31.0 291S375* (TCG→TAG) ttdTL‑tartrate/succinate antiporte
Reads supporting (aligned to +/- strand):  ref base C (133/142);  new base A (10/6);  total (143/148)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 293 total aligned reads displayed.

ATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGC  >  CP009273/3202358‑3202654
                                                                                                                                                    |                                                                                                                                                    
   aGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGcc                                                                                                                                                   <  2:4322595/149‑1 (MQ=255)
   aGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGcc                                                                                                                                                   <  2:2112569/149‑1 (MQ=255)
       gggACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACg                                                                                                                                               <  1:2335586/149‑1 (MQ=255)
        ggACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACga                                                                                                                                              <  1:2238307/149‑1 (MQ=255)
            gacATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggt                                                                                                                                          <  2:4352022/149‑1 (MQ=255)
            gacATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggt                                                                                                                                          <  1:4822628/149‑1 (MQ=255)
            gacATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACgatggt                                                                                                                                          >  1:1158072/1‑149 (MQ=255)
             acATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtg                                                                                                                                         >  2:3883165/1‑149 (MQ=255)
              cATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtga                                                                                                                                        <  1:3672119/149‑1 (MQ=255)
               aTTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtgat                                                                                                                                       >  1:3146259/1‑149 (MQ=255)
                ttGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtgatg                                                                                                                                      >  1:2899265/1‑149 (MQ=255)
                 tGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACgatggtgatgg                                                                                                                                     <  1:2761220/149‑1 (MQ=255)
                       taataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGt                                                                                                                               <  2:924325/149‑1 (MQ=255)
                       taataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGt                                                                                                                               <  1:1900392/149‑1 (MQ=255)
                         ataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTg                                                                                                                             >  2:4215016/1‑149 (MQ=255)
                         ataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTg                                                                                                                             <  1:5070488/149‑1 (MQ=255)
                         ataaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTg                                                                                                                             <  2:231743/149‑1 (MQ=255)
                          taaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCACCGATGGTGATGGTGGCGTGGa                                                                                                                            >  2:1002910/1‑149 (MQ=255)
                          taaAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGa                                                                                                                            <  1:4742724/149‑1 (MQ=255)
                           aaaGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGAt                                                                                                                           >  1:4821383/1‑149 (MQ=255)
                            aaGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGAtt                                                                                                                          <  2:1747444/149‑1 (MQ=255)
                            aaGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGAtt                                                                                                                          >  1:2462409/1‑149 (MQ=255)
                             aGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATtg                                                                                                                         >  2:553875/1‑149 (MQ=255)
                             aGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATtg                                                                                                                         <  1:642694/149‑1 (MQ=255)
                                 gcgTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTgtggt                                                                                                                     <  2:4683798/149‑1 (MQ=255)
                                   gTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGgtgt                                                                                                                   >  2:3400965/1‑149 (MQ=255)
                                   gTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGGTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGgtgt                                                                                                                   >  2:1516992/1‑149 (MQ=255)
                                    tGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGtt                                                                                                                  <  2:1321909/149‑1 (MQ=255)
                                    tGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGtt                                                                                                                  >  1:2591453/1‑149 (MQ=255)
                                     ggAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGttt                                                                                                                 <  1:1265550/149‑1 (MQ=255)
                                     ggAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGttt                                                                                                                 >  1:3127160/1‑149 (MQ=255)
                                      gAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGtttt                                                                                                                >  1:4084698/1‑149 (MQ=255)
                                      gAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGtttt                                                                                                                >  1:1488775/1‑149 (MQ=255)
                                        aCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTAt                                                                                                              <  1:1443318/149‑1 (MQ=255)
                                        aCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTAt                                                                                                              <  1:3329906/149‑1 (MQ=255)
                                        aCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTAt                                                                                                              >  1:4968910/1‑149 (MQ=255)
                                        aCGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTAt                                                                                                              >  2:5045708/1‑149 (MQ=255)
                                        aCGTTGTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTAt                                                                                                              <  2:34848/149‑1 (MQ=255)
                                          gTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCt                                                                                                            <  2:2012670/149‑1 (MQ=255)
                                           ttttCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTa                                                                                                           <  1:5164974/149‑1 (MQ=255)
                                           ttttCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTa                                                                                                           >  2:1777792/1‑149 (MQ=255)
                                           ttttCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTa                                                                                                           <  1:2230935/149‑1 (MQ=255)
                                           ttttCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTa                                                                                                           >  1:3590341/1‑149 (MQ=255)
                                             ttcttcTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACt                                                                                                         >  1:872534/1‑149 (MQ=255)
                                              tcttctGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTg                                                                                                        <  1:1939504/149‑1 (MQ=255)
                                                   tggctggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTa                                                                                                   >  1:935557/1‑149 (MQ=255)
                                                   tggctggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTa                                                                                                   >  1:2159333/1‑149 (MQ=255)
                                                    ggctggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTAc                                                                                                  <  2:2449743/149‑1 (MQ=255)
                                                     gctggcCTCGCTTATCACCCTCGCCACCGGACTCAACATCACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGTCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACt                                                                                                 <  2:434849/149‑1 (MQ=255)
                                                     gctggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACt                                                                                                 >  2:1626328/1‑149 (MQ=255)
                                                       tggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACttt                                                                                               <  2:3130292/149‑1 (MQ=255)
                                                       tggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACttt                                                                                               <  2:2988550/149‑1 (MQ=255)
                                                       tggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACttt                                                                                               >  2:2335812/1‑149 (MQ=255)
                                                        ggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACtttt                                                                                              >  1:4298279/1‑149 (MQ=255)
                                                        ggcCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACtttt                                                                                              >  1:2015083/1‑149 (MQ=255)
                                                        ggcCTCGCTTATCACCCCCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACtttt                                                                                              >  2:5170222/1‑149 (MQ=255)
                                                          ccTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTGTTTc                                                                                            >  2:4613387/1‑149 (MQ=255)
                                                            tCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTCAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGc                                                                                          >  2:287971/1‑149 (MQ=255)
                                                             cGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGcc                                                                                         >  1:3619247/1‑149 (MQ=255)
                                                                 tATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAgcg                                                                                     >  2:4413609/1‑149 (MQ=255)
                                                                           gCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGta                                                                           <  1:1043778/149‑1 (MQ=255)
                                                                            ccACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGTCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGtat                                                                          <  2:463256/149‑1 (MQ=255)
                                                                               ccGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATAcc                                                                       <  2:3091108/149‑1 (MQ=255)
                                                                                  gACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTcc                                                                    >  2:2149008/1‑149 (MQ=255)
                                                                                  gACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTcc                                                                    <  2:1162696/149‑1 (MQ=255)
                                                                                  gACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTcc                                                                    <  2:3758523/149‑1 (MQ=255)
                                                                                      caacaaCACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGctc                                                                <  2:3494624/149‑1 (MQ=255)
                                                                                       aacaacACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGctct                                                               <  2:1257053/149‑1 (MQ=255)
                                                                                        acaacaCCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCtctc                                                              <  1:3252892/149‑1 (MQ=255)
                                                                                            cacCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCAc                                                          >  1:1323301/1‑149 (MQ=255)
                                                                                               cGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCga                                                       <  2:2045181/149‑1 (MQ=255)
                                                                                                 gTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatg                                                     >  2:1078928/1‑149 (MQ=255)
                                                                                                  ttttATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatga                                                    >  1:3817913/1‑149 (MQ=255)
                                                                                                    ttattaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatg                                                  <  2:3282314/149‑1 (MQ=255)
                                                                                                    ttattaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatg                                                  <  2:4821383/149‑1 (MQ=255)
                                                                                                     tattaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatga                                                 >  1:715789/1‑149 (MQ=255)
                                                                                                     tattaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatga                                                 <  2:4790249/149‑1 (MQ=255)
                                                                                                      attaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCgatgatgat                                                >  2:1352799/1‑149 (MQ=255)
                                                                                                       ttaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGAtt                                               >  1:209989/1‑149 (MQ=255)
                                                                                                       ttaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGAtt                                               >  1:979682/1‑149 (MQ=255)
                                                                                                       ttaGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTAGCACCGATGATGAtt                                               <  1:4448912/149‑1 (MQ=255)
                                                                                                         aGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgc                                             <  2:777265/149‑1 (MQ=255)
                                                                                                            tGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgctgc                                          >  1:1287826/1‑149 (MQ=255)
                                                                                                            tGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgctgc                                          <  1:2391554/149‑1 (MQ=255)
                                                                                                             ggTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgctgct                                         <  2:696420/149‑1 (MQ=255)
                                                                                                               tttGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATtgctgctgc                                       <  2:3476881/149‑1 (MQ=255)
                                                                                                                      aaCTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCg                                >  1:1812543/1‑149 (MQ=255)
                                                                                                                        cTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGAt                              <  2:2796442/149‑1 (MQ=255)
                                                                                                                          gTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGc                            <  1:3957189/149‑1 (MQ=255)
                                                                                                                              gcaggcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGa                        >  1:4485850/1‑149 (MQ=255)
                                                                                                                                aggcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGaaa                      <  1:2960795/149‑1 (MQ=255)
                                                                                                                                  gcagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATc                    <  2:1443580/149‑1 (MQ=255)
                                                                                                                                   cagCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATcc                   <  1:4215895/149‑1 (MQ=255)
                                                                                                                                     gCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCg                 >  1:3229116/1‑149 (MQ=255)
                                                                                                                                      cTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGc                <  2:4969829/149‑1 (MQ=255)
                                                                                                                                      cTTAAGCGGTTATTAGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGc                >  2:4089514/1‑149 (MQ=255)
                                                                                                                                             ggTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACATTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGgt         >  1:3461015/1‑149 (MQ=255)
                                                                                                                                                tATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGtt      <  2:3538992/149‑1 (MQ=255)
                                                                                                                                                 aTTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTc     <  2:3330352/149‑1 (MQ=255)
                                                                                                                                                  ttCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCt    >  1:1687908/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCTGGCTGGCCTCGCTTATCACCCTCGCCACCGGACTCAACAACACCGGTTTTATTAGCTGGTTTGGCAAACTGTTAGCAGGCAGCTTAAGCGGTTATTCGCCAACGATGGTGATGGTGGCGTTGATTGTGGTGTTTTATCTACTGCGCTACTTTTTCGCCAGCGCCACGGCGTATACCTCCGCTCTCGCACCGATGATGATTGCTGCTGCGCTGGCGATGCCGGAAATCCCGCTGCCGGTGTTCTGC  >  CP009273/3202358‑3202654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑