Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,267,619 G→T 5.7% I216I (ATC→ATA)  garP ← putative (D)‑galactarate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,267,6190GT 5.7% 1075.2 / 29.7 277I216I (ATC→ATA) garPputative (D)‑galactarate transporter
Reads supporting (aligned to +/- strand):  ref base G (130/130);  new base T (7/10);  total (137/140)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

TGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTCAGCCAGCCAAGCA  >  CP009273/3267471‑3267767
                                                                                                                                                    |                                                                                                                                                    
tGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCAACGCGCCATTTTCagag                                                                                                                                                      >  1:523093/1‑149 (MQ=255)
 gATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAgaga                                                                                                                                                     <  1:328076/149‑1 (MQ=255)
  aTGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGAt                                                                                                                                                    >  2:4458132/1‑149 (MQ=255)
   tGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATa                                                                                                                                                   <  2:4302166/149‑1 (MQ=255)
   tGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATa                                                                                                                                                   <  1:342862/149‑1 (MQ=255)
   tGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATa                                                                                                                                                   >  1:769953/1‑149 (MQ=255)
   tGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATa                                                                                                                                                   >  1:1625246/1‑149 (MQ=255)
     gtgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATaaa                                                                                                                                                 <  2:1450501/149‑1 (MQ=255)
      tgtTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAAc                                                                                                                                                <  1:3957311/149‑1 (MQ=255)
         tGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTc                                                                                                                                             <  1:2427182/149‑1 (MQ=255)
           aTAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAg                                                                                                                                           >  2:332177/1‑149 (MQ=255)
               aaTATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGctct                                                                                                                                       <  1:1425264/149‑1 (MQ=255)
                 tatTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTc                                                                                                                                     <  2:1817303/149‑1 (MQ=255)
                 tatTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTc                                                                                                                                     <  1:1344194/149‑1 (MQ=255)
                  atTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                                                                                                    <  2:3911230/149‑1 (MQ=255)
                  atTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTcc                                                                                                                                    <  2:3058960/149‑1 (MQ=255)
                      tCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAg                                                                                                                                >  2:212589/1‑149 (MQ=255)
                         gAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACa                                                                                                                             >  1:3527680/1‑149 (MQ=255)
                          aaaaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACAt                                                                                                                            >  1:4636740/1‑149 (MQ=255)
                          aaaaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACAt                                                                                                                            >  1:2042488/1‑149 (MQ=255)
                           aaaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                                                                                                           >  1:4931367/1‑149 (MQ=255)
                           aaaaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATa                                                                                                                           >  1:2779418/1‑149 (MQ=255)
                             aaTACGCCCAGCATCATGCGGTTCGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACg                                                                                                                         >  1:4595176/1‑149 (MQ=255)
                             aaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCTACCACCGCGCCATTTTAAGATATAAACTTCAGCTCTTCAGCAGACATAct                                                                                                                         >  2:3765280/1‑148 (MQ=255)
                             aaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACg                                                                                                                         >  1:1082177/1‑149 (MQ=255)
                             aaTACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACg                                                                                                                         >  2:4903249/1‑149 (MQ=255)
                               tACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTg                                                                                                                       >  1:612671/1‑149 (MQ=255)
                                 cGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGTGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTggg                                                                                                                     <  2:3133211/149‑1 (MQ=255)
                                  gCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGt                                                                                                                    <  1:4505594/149‑1 (MQ=255)
                                    ccAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGATATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGa                                                                                                                  >  2:2441728/1‑149 (MQ=255)
                                      aGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATc                                                                                                                <  1:1549590/149‑1 (MQ=255)
                                         atcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGt                                                                                                             <  1:3040871/149‑1 (MQ=255)
                                         atcatGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGt                                                                                                             <  2:2670785/149‑1 (MQ=255)
                                            atGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCgg                                                                                                          <  2:619981/149‑1 (MQ=255)
                                            atGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCgg                                                                                                          >  2:4943434/1‑149 (MQ=255)
                                            atGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCgg                                                                                                          <  2:246172/149‑1 (MQ=255)
                                             tGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCggg                                                                                                         <  2:1322465/149‑1 (MQ=255)
                                              gCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGt                                                                                                        <  1:828537/149‑1 (MQ=255)
                                              gCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGt                                                                                                        <  1:4380721/149‑1 (MQ=255)
                                               cGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGtt                                                                                                       <  2:3363241/149‑1 (MQ=255)
                                                ggTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTa                                                                                                      >  1:871001/1‑149 (MQ=255)
                                                ggTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTa                                                                                                      <  2:1781109/149‑1 (MQ=255)
                                                 gTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTAt                                                                                                     >  1:3739036/1‑149 (MQ=255)
                                                 gTTAGAGAGCAATTGCGTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTAt                                                                                                     <  2:881056/149‑1 (MQ=255)
                                                   tAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGa                                                                                                   >  2:1971004/1‑149 (MQ=255)
                                                    agagagCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGaa                                                                                                  >  2:3901262/1‑149 (MQ=255)
                                                     gagagCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAAt                                                                                                 >  2:322242/1‑149 (MQ=255)
                                                      agagCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATc                                                                                                <  1:4288048/149‑1 (MQ=255)
                                                       gagCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCa                                                                                               <  2:1891649/149‑1 (MQ=255)
                                                       gagCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATAGACAACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTACGCAGAAAAACGTGGGTGATCTTTCGGGTTATGAAtaa                                                                                               >  1:3167464/1‑147 (MQ=255)
                                                           aaTTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACtt                                                                                           >  1:1243036/1‑149 (MQ=255)
                                                           aaTTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACtt                                                                                           <  1:2737121/149‑1 (MQ=255)
                                                             ttGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGa                                                                                         >  1:4228764/1‑149 (MQ=255)
                                                             ttGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGa                                                                                         >  1:3842759/1‑149 (MQ=255)
                                                                 ttGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCca                                                                                     <  1:4802939/149‑1 (MQ=255)
                                                                  tGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCcac                                                                                    <  2:1646816/149‑1 (MQ=255)
                                                                    aTGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAg                                                                                  <  1:4092315/149‑1 (MQ=255)
                                                                     tGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAgc                                                                                 >  1:4710202/1‑149 (MQ=255)
                                                                       tAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAgcgc                                                                               >  2:364762/1‑149 (MQ=255)
                                                                               tttGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGca                                                                       >  2:4107967/1‑149 (MQ=255)
                                                                               tttGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGca                                                                       >  2:1043421/1‑149 (MQ=255)
                                                                               tttGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGca                                                                       >  1:1608122/1‑149 (MQ=255)
                                                                               tttGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGca                                                                       >  2:3475853/1‑149 (MQ=255)
                                                                                 tGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGcaca                                                                     >  2:2082470/1‑149 (MQ=255)
                                                                                   ggTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACaaa                                                                   >  1:935852/1‑149 (MQ=255)
                                                                                          ttGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGATATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATc                                                            <  1:1659814/149‑1 (MQ=255)
                                                                                          ttGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATc                                                            >  1:3650278/1‑149 (MQ=255)
                                                                                          ttGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATc                                                            >  1:2530475/1‑149 (MQ=255)
                                                                                               gcCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCAcccc                                                       <  2:4457227/149‑1 (MQ=255)
                                                                                               gcCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCAcccc                                                       <  1:2166173/149‑1 (MQ=255)
                                                                                                    aCTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATaa                                                  <  1:2600841/149‑1 (MQ=255)
                                                                                                     cTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAAc                                                 >  2:4213042/1‑149 (MQ=255)
                                                                                                      tGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACg                                                >  1:1157746/1‑149 (MQ=255)
                                                                                                        cccGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGt                                              <  2:756684/149‑1 (MQ=255)
                                                                                                           ggCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTaaa                                           >  1:1920433/1‑149 (MQ=255)
                                                                                                             cTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAAACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGa                                         <  2:1226941/149‑1 (MQ=255)
                                                                                                              tttttGTGGTACAGATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGAc                                        <  2:5063670/149‑1 (MQ=255)
                                                                                                                 ttGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTg                                     >  2:1410000/1‑149 (MQ=255)
                                                                                                                     ggTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTcc                                 <  1:2348668/149‑1 (MQ=255)
                                                                                                                        ccATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAg                              >  1:4856833/1‑149 (MQ=255)
                                                                                                                          atatCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGcc                            <  2:4535343/149‑1 (MQ=255)
                                                                                                                            atCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGcccc                          <  1:1982578/149‑1 (MQ=255)
                                                                                                                              cGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAg                        >  2:450068/1‑149 (MQ=255)
                                                                                                                                 ccaccGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCg                     >  2:2824167/1‑149 (MQ=255)
                                                                                                                                   accGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGaa                   <  1:4080974/149‑1 (MQ=255)
                                                                                                                                    ccGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGaaa                  >  2:532658/1‑149 (MQ=255)
                                                                                                                                    ccGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGaaa                  >  1:2119989/1‑149 (MQ=255)
                                                                                                                                     cgcgCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAg                 <  1:3802246/149‑1 (MQ=255)
                                                                                                                                       cgcCATTTTCAGATAGAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTc               <  2:4657847/149‑1 (MQ=255)
                                                                                                                                        gcCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTca              >  2:1514900/1‑149 (MQ=255)
                                                                                                                                        gcCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTca              <  1:697252/149‑1 (MQ=255)
                                                                                                                                         ccATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTcag             <  1:1510273/149‑1 (MQ=255)
                                                                                                                                         ccATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTcag             <  1:1508716/149‑1 (MQ=255)
                                                                                                                                           aTTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTcagcc           <  1:1841341/149‑1 (MQ=255)
                                                                                                                                           aTTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTcagcc           >  1:2731028/1‑149 (MQ=255)
                                                                                                                                              ttCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTcagccagc        <  2:77988/149‑1 (MQ=255)
                                                                                                                                                   agaTAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTCAGCCAGCCAagc   >  2:4801062/1‑149 (MQ=255)
                                                                                                                                                   agaTAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTCAGCCAGCCAagc   >  1:2454271/1‑149 (MQ=255)
                                                                                                                                                    gaTAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTCAGCCAGCCAagca  >  2:49821/1‑149 (MQ=255)
                                                                                                                                                    gaTAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTCAGCCAGCCAagca  >  1:2867565/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGATGGTGTTGATAAAATATTGTCCGAAAAATACGCCCAGCATCATGCGGTTAGAGAGCAATTGCTTGATGTAATGCAGTTTGGGTCCGCTTGCTGCCGCACTGCCCGGCTTTTTGTGGTCCATATCGACCACCGCGCCATTTTCAGAGATAAACTTCAGCTCTTCCGCAGACATACGTGGGTGATCTGTCGGGTTATGAATCAACTTGATCCACAGCGCCGTCAGCACAAAACCAATCACCCCCATAACGGTAAAGACGTGCTCCCAGCCCCAGGCGAAAGTCAGCCAGCCAAGCA  >  CP009273/3267471‑3267767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑