Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,531,954 A→T 9.3% Q404L (CAG→CTG)  yhgF → putative transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,531,9540AT 9.3% 1224.0 / 90.2 345Q404L (CAG→CTG) yhgFputative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base A (186/127);  new base T (21/11);  total (207/138)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

GACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATC  >  CP009273/3531806‑3532100
                                                                                                                                                    |                                                                                                                                                  
     cATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTcc                                                                                                                                               <  1:2250848/149‑1 (MQ=255)
       tttACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCg                                                                                                                                             >  2:4851498/1‑149 (MQ=255)
       tttACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCg                                                                                                                                             >  1:1985246/1‑149 (MQ=255)
         tACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGaa                                                                                                                                           >  1:4683115/1‑149 (MQ=255)
           cccGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAg                                                                                                                                         <  2:577025/149‑1 (MQ=255)
            ccGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGt                                                                                                                                        <  1:2353948/149‑1 (MQ=255)
                acacCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGAcc                                                                                                                                    >  1:290718/1‑149 (MQ=255)
                acacCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGAcc                                                                                                                                    >  2:71076/1‑149 (MQ=255)
                  acCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACcgc                                                                                                                                  >  1:3837070/1‑149 (MQ=255)
                      gACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAg                                                                                                                              >  1:3498149/1‑149 (MQ=255)
                      gACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAg                                                                                                                              >  2:3980642/1‑149 (MQ=255)
                         aGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGaaa                                                                                                                           >  1:2394068/1‑149 (MQ=255)
                         aGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGaaa                                                                                                                           >  1:822401/1‑149 (MQ=255)
                           gCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGt                                                                                                                         <  1:4044704/149‑1 (MQ=255)
                            ccGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTa                                                                                                                        <  2:2879655/149‑1 (MQ=255)
                             cGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTaa                                                                                                                       <  1:3016738/149‑1 (MQ=255)
                             cGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTaa                                                                                                                       <  1:5011628/149‑1 (MQ=255)
                              gCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAAt                                                                                                                      <  1:1025267/149‑1 (MQ=255)
                              gCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAAt                                                                                                                      <  2:347061/149‑1 (MQ=255)
                               cAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATc                                                                                                                     >  2:851970/1‑149 (MQ=255)
                                  aaGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTc                                                                                                                  <  2:4683115/149‑1 (MQ=255)
                                     cagcGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGc                                                                                                               >  2:3757039/1‑149 (MQ=255)
                                      agcGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCg                                                                                                              >  1:1739913/1‑149 (MQ=255)
                                       gcGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGa                                                                                                             >  1:1784769/1‑149 (MQ=255)
                                       gcGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGa                                                                                                             >  1:1312006/1‑149 (MQ=255)
                                           tGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCa                                                                                                         >  2:3185289/1‑149 (MQ=255)
                                             aCCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAgg                                                                                                       >  2:4051587/1‑149 (MQ=255)
                                             aCCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAgg                                                                                                       <  2:2376385/149‑1 (MQ=255)
                                               cGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGgcg                                                                                                     >  1:4990452/1‑149 (MQ=255)
                                               cGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGgcg                                                                                                     >  1:4167845/1‑149 (MQ=255)
                                                   gctgcGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTc                                                                                                 >  1:1951879/1‑149 (MQ=255)
                                                      gcgcTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGt                                                                                              >  1:892267/1‑149 (MQ=255)
                                                       cgcTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGtt                                                                                             <  1:2847347/149‑1 (MQ=255)
                                                        gcTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGttt                                                                                            >  2:3678737/1‑149 (MQ=255)
                                                        gcTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGttt                                                                                            <  1:2207106/149‑1 (MQ=255)
                                                          tgtgtgAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTAc                                                                                          >  2:2536102/1‑149 (MQ=255)
                                                           gtgtgAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACt                                                                                         <  1:4267628/149‑1 (MQ=255)
                                                            tgtgAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTc                                                                                        <  2:2008872/149‑1 (MQ=255)
                                                             gtgAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCg                                                                                       <  2:3149279/149‑1 (MQ=255)
                                                             gtgAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCg                                                                                       >  2:2152928/1‑149 (MQ=255)
                                                             gtgAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCg                                                                                       >  1:3081352/1‑149 (MQ=255)
                                                              tgAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCgg                                                                                      >  1:3997080/1‑149 (MQ=255)
                                                                aaaaaCATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCt                                                                                    <  1:1354324/149‑1 (MQ=255)
                                                                 aaaaCATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCtt                                                                                   >  2:4425602/1‑149 (MQ=255)
                                                                  aaaCATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTc                                                                                  >  1:2151659/1‑149 (MQ=255)
                                                                   aaCATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTcc                                                                                 <  1:411790/149‑1 (MQ=255)
                                                                    aCATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCg                                                                                >  1:445436/1‑149 (MQ=255)
                                                                    aCATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCg                                                                                <  2:1407789/149‑1 (MQ=255)
                                                                    aCATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCg                                                                                >  1:3342274/1‑149 (MQ=255)
                                                                     aaTAACGTTGAACTGGTAGCGATCGGTAACTGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGa                                                                               <  2:3957995/148‑1 (MQ=255)
                                                                       tAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGAGGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAgc                                                                             <  2:1504888/149‑1 (MQ=255)
                                                                        aaCGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAgct                                                                            <  2:1055432/149‑1 (MQ=255)
                                                                         aCGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAgctg                                                                           >  2:1745142/1‑149 (MQ=255)
                                                                           gTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAgctggc                                                                         <  1:4826755/149‑1 (MQ=255)
                                                                           gTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAgctggc                                                                         <  1:995393/149‑1 (MQ=255)
                                                                                    gTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGa                                                                <  1:142873/149‑1 (MQ=255)
                                                                                    gTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGa                                                                <  1:122942/149‑1 (MQ=255)
                                                                                      aGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGt                                                              >  1:3139640/1‑149 (MQ=255)
                                                                                       gCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGtt                                                             >  1:4429406/1‑149 (MQ=255)
                                                                                       gCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGtt                                                             >  2:1834576/1‑149 (MQ=255)
                                                                                       gCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGtt                                                             >  2:134582/1‑149 (MQ=255)
                                                                                          aTCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTccc                                                          >  1:11903/1‑149 (MQ=255)
                                                                                          aTCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTccc                                                          <  2:3491763/149‑1 (MQ=255)
                                                                                          aTCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTccc                                                          <  1:606410/149‑1 (MQ=255)
                                                                                          aTCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTccc                                                          >  1:2316823/1‑149 (MQ=255)
                                                                                             ggTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGa                                                       <  1:2707178/149‑1 (MQ=255)
                                                                                             ggTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGa                                                       >  1:1582768/1‑149 (MQ=255)
                                                                                               tAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGAtc                                                     >  2:4114781/1‑149 (MQ=255)
                                                                                               tAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGAtc                                                     <  2:2713888/149‑1 (MQ=255)
                                                                                                aaCGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGAtct                                                    >  1:4662228/1‑149 (MQ=255)
                                                                                                 aCGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGAtctc                                                   >  2:2090411/1‑149 (MQ=255)
                                                                                                       cAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGtt                                             <  1:5028718/149‑1 (MQ=255)
                                                                                                       cAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGtt                                             <  1:3185047/149‑1 (MQ=255)
                                                                                                        aGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGttt                                            >  1:2481440/1‑149 (MQ=255)
                                                                                                        aGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGtgt                                            >  2:4974603/1‑147 (MQ=255)
                                                                                                         gCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTc                                           >  2:4995407/1‑149 (MQ=255)
                                                                                                         gCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTc                                           >  1:962778/1‑149 (MQ=255)
                                                                                                           ttCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGc                                         <  1:2023155/149‑1 (MQ=255)
                                                                                                           ttCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGc                                         <  1:3944369/149‑1 (MQ=255)
                                                                                                            tCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCt                                        >  1:1911403/1‑149 (MQ=255)
                                                                                                             cccGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTg                                       >  1:4603323/1‑149 (MQ=255)
                                                                                                                 cgAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAGGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTg                                   <  2:936137/149‑1 (MQ=255)
                                                                                                                  gAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTgg                                  <  2:3213735/149‑1 (MQ=255)
                                                                                                                  gAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTgg                                  >  2:4609741/1‑149 (MQ=255)
                                                                                                                   gaaCTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGgc                                 >  1:767147/2‑149 (MQ=255)
                                                                                                                     aCTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGgcgc                               >  1:4332037/1‑149 (MQ=255)
                                                                                                                        gAGCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGgt                            <  1:623394/149‑1 (MQ=255)
                                                                                                                        gAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGgt                            >  1:3727092/1‑149 (MQ=255)
                                                                                                                          gCGTTTCTATCTCGATGTGCAGAAGCTGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGgtgt                          <  2:126738/149‑1 (MQ=255)
                                                                                                                              ttCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTAt                      >  1:3325466/1‑149 (MQ=255)
                                                                                                                                    ctcGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATcgcccg                <  1:5067947/149‑1 (MQ=255)
                                                                                                                                       gATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCccgccg             >  1:2593679/1‑149 (MQ=255)
                                                                                                                                          gtgCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGttt          >  2:223582/1‑149 (MQ=255)
                                                                                                                                            gCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGc        >  1:1647595/1‑149 (MQ=255)
                                                                                                                                            gCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGc        >  1:3610188/1‑149 (MQ=255)
                                                                                                                                               gAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAgg     >  2:1800991/1‑149 (MQ=255)
                                                                                                                                                 aGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGAt   >  2:2874013/1‑149 (MQ=255)
                                                                                                                                                 aGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGAt   <  2:3837070/149‑1 (MQ=255)
                                                                                                                                                  gCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTGTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATc  <  2:4351826/149‑1 (MQ=255)
                                                                                                                                                  gCAGTTACCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATc  <  1:4627331/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GACACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGTAGCGATCGGTAACGGTACAGCTTCCCGCGAAACTGAGCGTTTCTATCTCGATGTGCAGAAGCAGTTCCCGAAAGTGACCGCGCAGAAAGTAATCGTCAGCGAAGCAGGCGCGTCGGTTTACTCGGCTTCCGAGCTGGCTGCACAGGAGTTCCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATC  >  CP009273/3531806‑3532100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑