Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,480 T→G 7.4% T418P (ACG→CCG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4800TG 7.4% 733.2 / 7.1 279T418P (ACG→CCG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (96/154);  new base G (27/2);  total (123/156)
Fisher's exact test for biased strand distribution p-value = 6.78e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

GCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  CP009273/3721333‑3721627
                                                                                                                                                   |                                                                                                                                                   
 cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTAc                                                                                                                                                   <  2:1064354/149‑1 (MQ=255)
   gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACgg                                                                                                                                                 >  2:1279698/1‑149 (MQ=255)
    aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                <  1:1411297/149‑1 (MQ=255)
    aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                <  1:3887618/149‑1 (MQ=255)
      aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTaa                                                                                                                                              <  1:3274719/149‑1 (MQ=255)
      aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTaa                                                                                                                                              <  2:3769581/149‑1 (MQ=255)
       aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAt                                                                                                                                             <  2:5002252/149‑1 (MQ=255)
            cATAACGCTGCTCATCTGGTAGATGCGTCTGTTCTAACGGTTGGTGCTGCATCAATTCAATGAGCGTTTTCTCTGGATTCGCCGAGATCTTCGCCAGCCTTGCTGCGCCCAGTTCTGGCCCCACATCCCACCCCGTACGTTAATCGAgc                                                                                                                                        <  2:3433887/149‑1 (MQ=255)
            cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                        <  1:5001081/149‑1 (MQ=255)
            cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                        <  1:2695986/149‑1 (MQ=255)
            cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                        <  2:2701122/149‑1 (MQ=255)
             aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGTGCGGTCATCGAgcg                                                                                                                                       >  1:3475982/1‑148 (MQ=255)
              tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTGCGGGAATCGAgctg                                                                                                                                      >  1:1753573/1‑149 (MQ=255)
               aaCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTGCGGGAAGGGCgctgc                                                                                                                                     >  2:4923668/1‑149 (MQ=255)
                aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                    <  1:3353616/149‑1 (MQ=255)
                aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCGCCGCCCGGACGGGGTTCGAgctgct                                                                                                                                    >  1:2017185/1‑149 (MQ=255)
                      cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                              <  2:2838529/149‑1 (MQ=255)
                      cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                              <  2:1220742/149‑1 (MQ=255)
                       gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                             <  1:438469/149‑1 (MQ=255)
                        cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                                                            <  2:2265027/149‑1 (MQ=255)
                         aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGACGGTAATCGAGCTGCTGACCGcggg                                                                                                                           >  2:1997075/1‑146 (MQ=255)
                           cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                         <  2:4254464/149‑1 (MQ=255)
                            tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGTACGGTAATCGAGCTGGTGACCGCTGaggt                                                                                                                        >  1:469757/1‑146 (MQ=255)
                            tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCGGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                                                        <  2:4176667/149‑1 (MQ=255)
                                 gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGTCGGTAAGCGCGCTGCTGACCGCTGAGGTCCGcc                                                                                                                   >  1:2518811/1‑149 (MQ=255)
                                 gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCGGACCGCTGATATCCGcc                                                                                                                   >  1:5172912/1‑149 (MQ=255)
                                  aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                  <  2:4573137/149‑1 (MQ=255)
                                     cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                                                                                               <  2:3631493/149‑1 (MQ=255)
                                      gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGTCCTCGAGCTGCTGCCCGCGGATCTCGGCCCGcgg                                                                                                              >  1:3191652/1‑147 (MQ=255)
                                      gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCCCCGTGGGGGAGTCGTGCTGCTGGGCGCGGATGTCCGCCAGccg                                                                                                              >  2:1791514/1‑147 (MQ=255)
                                       aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                             <  1:1010917/149‑1 (MQ=255)
                                       aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGCGGGCCGCGAGCTGCTGAGCGCTGTGATCCGCCGGGATc                                                                                                             >  2:2830312/1‑149 (MQ=255)
                                              tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                      <  1:3879651/149‑1 (MQ=255)
                                               aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTAGGGTGGTCGTGCTGGTGACCGCTGGTATCCGCCGGCAGGTGACGGCa                                                                                                     >  1:2116255/1‑149 (MQ=255)
                                               aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTACTCGGGCGGCTGTCCGCTGTTTTGCGCCCGCCTCTGTCGccg                                                                                                     >  1:812301/1‑148 (MQ=255)
                                                aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                                                    <  2:2188894/149‑1 (MQ=255)
                                                  ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                                  <  1:3812042/149‑1 (MQ=255)
                                                   gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGGGCGGTCATCGAGCTGCTGACCGCGGATATCCGCCAGCGGCTGACGCCGGTAc                                                                                                 >  1:3995067/1‑149 (MQ=255)
                                                   gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGTGCGGGACTGGAGCTGCGGACCGCTGAGATCCGCCGGCTTCTGACGCCGGttc                                                                                                 >  2:41224/1‑147 (MQ=255)
                                                       ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                             <  1:895003/149‑1 (MQ=255)
                                                       ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                             <  1:1749722/149‑1 (MQ=255)
                                                       ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                             <  1:4606031/149‑1 (MQ=255)
                                                       ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                             <  1:3320900/149‑1 (MQ=255)
                                                        tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACt                                                                                            <  2:4746318/149‑1 (MQ=255)
                                                         gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                           <  1:4676253/149‑1 (MQ=255)
                                                                caaTTCACTGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                    <  2:519633/149‑1 (MQ=255)
                                                                caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTCCGGTACTGGAGCTGCTGGCCGGGGGTATCCGCCCGCATCTGGCGCCAGTACTCACGACGcgccc                                                                                    >  1:2918831/1‑149 (MQ=255)
                                                                caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                             >  2:1628948/1‑140 (MQ=255)
                                                                caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                             <  1:1628911/140‑1 (MQ=255)
                                                                 aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGGGGTGCGCGAGCTGCGGGGCGCGGAGGGCCGCGCGCATCTGAGGGCCGTGCGCACTAcgcgggcc                                                                                   >  1:4807377/1‑145 (MQ=255)
                                                                    tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                <  1:3070001/149‑1 (MQ=255)
                                                                     cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGTCATCGAGCTGGTGGCCGGGGTTGTCCGCCGGCGTCGGAGGCCAGTGCTCACGACGcgcgccgccc                                                                               >  2:4074481/1‑149 (MQ=255)
                                                                      aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGTCGGTAAGGGAGCTGCTGACCGCTGCTCTCCGCCCGCGTCTGGCGCCAGTACTCACGGCGCgccccgcccc                                                                              >  1:2840460/1‑149 (MQ=255)
                                                                      aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGCCGGGCAGGGAGCTGCGGGCCGCGGATATCCGCCCGCATCTGACGCCCGGACTCACGCCGCgccccgcccc                                                                              >  2:2967228/1‑149 (MQ=255)
                                                                       aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTCCGGGGATCGCGCTGCTGGCCGCTGATATCCGCCAGCCTCTGACGCCAGTACGCACTACGCGCCCCGccccc                                                                             >  2:4394444/1‑149 (MQ=255)
                                                                       aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                             <  1:4116418/149‑1 (MQ=255)
                                                                       aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGGGCTGCTGGCCGCTGATATCCGCCCGCATCTGACGCCAGGACTCACTCCGCGCCCCGccccc                                                                             >  1:3355768/1‑149 (MQ=255)
                                                                        tGAGCTATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                                            <  1:152806/149‑1 (MQ=255)
                                                                           gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                         <  2:4128123/149‑1 (MQ=255)
                                                                             gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGGGGGTGATCGAGCTGCTGTCCGCGGATAGCGGGCGGCATCTGACGGCAGTACGCTCTACGCGCCCCGcccccgcgcac                                                                       >  2:3231876/1‑143 (MQ=255)
                                                                              aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                      <  2:609058/149‑1 (MQ=255)
                                                                              aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCGGTCCGCGGATCTCCGCCAGCCGCGGACGCCAGGACTCACTACGCGCCCCGCCCCCCATcccc                                                                      >  1:4915961/1‑146 (MQ=255)
                                                                              aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGCCGCGAGCGGCGGCCCGCGGATATCCGCCCGCAGCGGACGCCAGTCCTCACTACGCGCCCCGccccccagcccc                                                                      >  1:1317628/1‑142 (MQ=255)
                                                                                  ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATCGAGCTGCTGGCGGCTGATATCCGCCAGCCTCTGACGCCAGTGCTCACTACGCGCCCCGCCCCCCATCAACggga                                                                  >  1:2360773/1‑146 (MQ=255)
                                                                                      ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACt                                                              <  1:1856901/149‑1 (MQ=255)
                                                                                       gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTGCGGTATTCGAGCTGCTGTCCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCCAGCAACGGAACACtt                                                             >  1:846270/1‑149 (MQ=255)
                                                                                       gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACtt                                                             <  2:265062/149‑1 (MQ=255)
                                                                                        aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                            <  1:1122076/149‑1 (MQ=255)
                                                                                         ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCGCCCCCGTACGGTAATCGAGCTGGTGACCGCTGATGTGCGCCAGCGTCTGTCGGCGGTTCTCAGTCCGCGCCCCGCCCCCACTCAGCGTAACACGTTg                                                           >  2:2768226/1‑149 (MQ=255)
                                                                                         ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                           <  1:1681329/149‑1 (MQ=255)
                                                                                         ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATTTCCGCCAGCATCTGGCGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTg                                                           >  1:2303248/1‑149 (MQ=255)
                                                                                          tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGTCATCGGGCTGCTGACCGCGGATATCCGCCCGCATCTGGCGCCAGTACTCTCTACGCGCCCCGCCCCCCATCAACGTACCACGTTGc                                                          >  1:1542761/1‑149 (MQ=255)
                                                                                          tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                          <  1:1175088/149‑1 (MQ=255)
                                                                                             ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGt                                                       <  1:518681/149‑1 (MQ=255)
                                                                                              cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                      <  1:4309667/149‑1 (MQ=255)
                                                                                                 gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGCATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCGGACGCCCGTACTCACTCCGCGCCCCGCCCCCCATCCACGGCACACGTTGCGGTTTaa                                                   >  1:4555223/1‑149 (MQ=255)
                                                                                                    cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                <  1:2729305/149‑1 (MQ=255)
                                                                                                        gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                                            <  2:2072835/149‑1 (MQ=255)
                                                                                                         ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATAGCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGGTAATACCGCAg                                           >  2:2345098/1‑149 (MQ=255)
                                                                                                              cTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGCTATCCGCCCGCCTCTGTCGCCGGTAGTCACTACGCGCCCCGCCCCCAATCCACGTAGCACTTTGCGGTTTAATACCGCAGGCATg                                      >  1:2852267/1‑149 (MQ=255)
                                                                                                              cTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTTACACTGTGCGGTTTAATACCGCAGGCATg                                      >  1:2294341/1‑149 (MQ=255)
                                                                                                               ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                     <  2:1183789/149‑1 (MQ=255)
                                                                                                               ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                     <  1:4353078/149‑1 (MQ=255)
                                                                                                               ttGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCCGGCATGc                                     >  1:3545223/1‑149 (MQ=255)
                                                                                                                     cgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                               >  1:3657834/1‑149 (MQ=255)
                                                                                                                       cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                             <  1:3289901/149‑1 (MQ=255)
                                                                                                                         cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa                           >  1:4036285/1‑149 (MQ=255)
                                                                                                                           gTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATCTCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTGTGCGGTTTAATACCGCAGGCCTGCCCGACAGCCATg                         >  2:4907022/1‑149 (MQ=255)
                                                                                                                              cTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                      <  1:1909399/149‑1 (MQ=255)
                                                                                                                                ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                    <  1:2431042/149‑1 (MQ=255)
                                                                                                                                ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                    <  2:4356155/149‑1 (MQ=255)
                                                                                                                                ggCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                    <  2:4710840/149‑1 (MQ=255)
                                                                                                                                 gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                   >  1:4481833/1‑149 (MQ=255)
                                                                                                                                    ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                <  2:1623656/149‑1 (MQ=255)
                                                                                                                                    ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACATTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcc                >  2:2694350/1‑149 (MQ=255)
                                                                                                                                     cacaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa               >  1:43055/1‑149 (MQ=255)
                                                                                                                                         tCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc           >  2:4332862/1‑149 (MQ=255)
                                                                                                                                          ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa          <  1:2238544/149‑1 (MQ=255)
                                                                                                                                           cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt         <  1:1279698/149‑1 (MQ=255)
                                                                                                                                           cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt         <  2:1086863/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCAC  >  CP009273/3721333‑3721627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑