BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,731,688	C→T	8.3%	Q278* (CAG→TAG)	malS →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,731,688	0	C	T	8.3%	1199.2 / 64.9	348	Q278* (CAG→TAG)	malS	alpha‑amylase
Reads supporting (aligned to +/- strand): ref base C (182/137); new base T (17/12); total (199/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 368 total aligned reads displayed.

ACGCGGCCTGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCAGGAGTA  >  CP009273/3731540‑3731836

  gcgGCCTGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAg                                                                                                                                                    <  1:499680/149‑1 (MQ=255)

     gCCTGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGAc                                                                                                                                                 >  1:4324184/1‑149 (MQ=255)

     gCCTGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGAc                                                                                                                                                 >  2:4724661/1‑149 (MQ=255)

       cTGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTg                                                                                                                                               <  2:1365034/149‑1 (MQ=255)

       cTGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTg                                                                                                                                               >  2:1010867/1‑149 (MQ=255)

        tGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTgg                                                                                                                                              >  2:3777910/1‑149 (MQ=255)

         gACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGa                                                                                                                                             <  1:927413/149‑1 (MQ=255)

             aacaaACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGaa                                                                                                                                         <  2:4563543/149‑1 (MQ=255)

                 aaCTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCtt                                                                                                                                     >  2:2390628/1‑149 (MQ=255)

                  aCTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTg                                                                                                                                    >  1:2414452/1‑149 (MQ=255)

                   cTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACATAGGACTGGACGAATCTTGa                                                                                                                                   >  2:3029353/1‑149 (MQ=255)

                    tGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGAt                                                                                                                                  >  2:4497532/1‑149 (MQ=255)

                         tACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCaa                                                                                                                             >  2:4806684/1‑149 (MQ=255)

                           ccTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAata                                                                                                                           >  1:3199527/1‑149 (MQ=255)

                            cTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAatat                                                                                                                          >  1:1023675/1‑149 (MQ=255)

                            cTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAatat                                                                                                                          >  2:2349311/1‑149 (MQ=255)

                             tCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATg                                                                                                                         <  1:5022459/149‑1 (MQ=255)

                             tCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATg                                                                                                                         <  2:781810/149‑1 (MQ=255)

                             tCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATg                                                                                                                         >  1:202265/1‑149 (MQ=255)

                               cagcagTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATggg                                                                                                                       <  2:3871186/149‑1 (MQ=255)

                                agcagTTGGGCGTTACTGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGc                                                                                                                      <  2:3410140/149‑1 (MQ=255)

                                agcagTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGc                                                                                                                      >  2:3289574/1‑149 (MQ=255)

                                agcagTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGc                                                                                                                      >  2:4634139/1‑149 (MQ=255)

                                 gcagTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCa                                                                                                                     <  1:2391111/149‑1 (MQ=255)

                                 gcagTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCa                                                                                                                     >  2:362008/1‑149 (MQ=255)

                                 gcagTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCa                                                                                                                     >  2:4961794/1‑149 (MQ=255)

                                   agTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAAc                                                                                                                   <  1:2723051/149‑1 (MQ=255)

                                   agTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAAc                                                                                                                   >  2:3248073/1‑149 (MQ=255)

                                    gTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACg                                                                                                                  >  1:810465/1‑149 (MQ=255)

                                    gTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATGTCCCGCATTATGCCTCCCACGGTGATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACg                                                                                                                  >  2:871467/1‑149 (MQ=255)

                                     ttGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGa                                                                                                                 <  1:3412354/149‑1 (MQ=255)

                                            ttAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGAt                                                                                                          <  2:4757978/149‑1 (MQ=255)

                                             tAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATc                                                                                                         >  2:2188210/1‑149 (MQ=255)

                                              aaTGCTTTATGGATAAGCGCCCCATTTGAGCACAGTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCt                                                                                                        <  2:1714377/149‑1 (MQ=255)

                                               aTGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTa                                                                                                       >  1:292875/1‑149 (MQ=255)

                                               aTGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTa                                                                                                       >  2:1552552/1‑149 (MQ=255)

                                               aTGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTa                                                                                                       <  1:1473764/149‑1 (MQ=255)

                                                tGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTAc                                                                                                      >  1:4058140/1‑149 (MQ=255)

                                                   tttATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGa                                                                                                   >  2:552106/1‑149 (MQ=255)

                                                    ttATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACATAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGAc                                                                                                  <  2:4910398/149‑1 (MQ=255)

                                                    ttATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGAc                                                                                                  >  2:2119434/1‑149 (MQ=255)

                                                      aTGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGc                                                                                                <  1:4163837/149‑1 (MQ=255)

                                                         gATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTgg                                                                                             >  1:3504468/1‑149 (MQ=255)

                                                             aGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGa                                                                                         <  1:1723681/149‑1 (MQ=255)

                                                              gcgcCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGAt                                                                                        >  2:1613834/1‑149 (MQ=255)

                                                              gcgcCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGAt                                                                                        <  2:3067679/149‑1 (MQ=255)

                                                                gcCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAg                                                                                      >  1:1574067/1‑149 (MQ=255)

                                                                gcCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAg                                                                                      <  2:4115956/149‑1 (MQ=255)

                                                                 ccccATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAga                                                                                     >  1:1873885/1‑148 (MQ=255)

                                                                    cATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGca                                                                                  >  2:3724626/1‑149 (MQ=255)

                                                                        tGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATc                                                                              <  2:2148004/149‑1 (MQ=255)

                                                                           gCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAgc                                                                           <  1:698454/149‑1 (MQ=255)

                                                                           gCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAgc                                                                           <  1:571397/149‑1 (MQ=255)

                                                                           gCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAgc                                                                           <  2:1508131/149‑1 (MQ=255)

                                                                               aTTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGACTCTTGATGCCCATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCgg                                                                       >  1:3746914/1‑149 (MQ=255)

                                                                               aTTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCgg                                                                       >  1:2172612/1‑149 (MQ=255)

                                                                                ttCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgt                                                                      <  1:4631358/149‑1 (MQ=255)

                                                                                ttCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgt                                                                      >  1:5125683/1‑149 (MQ=255)

                                                                                ttCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgt                                                                      >  1:1340204/1‑149 (MQ=255)

                                                                                 tCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgta                                                                     <  1:3777910/149‑1 (MQ=255)

                                                                                  cACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgtat                                                                    >  1:2868772/1‑149 (MQ=255)

                                                                                   aCGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACATAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgtatt                                                                   <  1:334424/149‑1 (MQ=255)

                                                                                     ggCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgtattcg                                                                 >  2:1753349/1‑149 (MQ=255)

                                                                                         gggTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGgtattcgtatt                                                             >  2:2507868/1‑149 (MQ=255)

                                                                                                 ggcggTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGa                                                     <  1:2225257/149‑1 (MQ=255)

                                                                                                  gcggTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACATAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGAt                                                    <  1:109101/149‑1 (MQ=255)

                                                                                                  gcggTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGAt                                                    <  1:1692148/149‑1 (MQ=255)

                                                                                                  gcggTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGAt                                                    >  2:4964913/1‑149 (MQ=255)

                                                                                                     gTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTc                                                 >  1:3018783/1‑149 (MQ=255)

                                                                                                     gTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTc                                                 >  1:4555114/1‑149 (MQ=255)

                                                                                                      tACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCg                                                <  2:2633143/149‑1 (MQ=255)

                                                                                                        cAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGtg                                              >  2:1096052/1‑149 (MQ=255)

                                                                                                         aaaaGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGtga                                             >  2:9480/1‑149 (MQ=255)

                                                                                                         aaaaGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGtga                                             <  2:2828766/149‑1 (MQ=255)

                                                                                                           aaGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGtgatg                                           >  2:4296559/1‑149 (MQ=255)

                                                                                                           aaGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGtgatg                                           >  2:4165973/1‑149 (MQ=255)

                                                                                                           aaGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGtgatg                                           <  2:1842496/149‑1 (MQ=255)

                                                                                                              gCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAAc                                        <  2:1978077/149‑1 (MQ=255)

                                                                                                                  tttCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACcaca                                    >  2:3559520/1‑149 (MQ=255)

                                                                                                                    tCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACAcc                                  >  2:2740858/1‑149 (MQ=255)

                                                                                                                         cATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTa                             <  2:889924/149‑1 (MQ=255)

                                                                                                                            tATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCATGATGAACCACACCGGCTATGc                          >  2:2904907/1‑149 (MQ=255)

                                                                                                                             aggCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGcc                         <  1:3527169/147‑1 (MQ=255)

                                                                                                                                 cTACCACGGTTATTACACATAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGc                     <  1:4862242/149‑1 (MQ=255)

                                                                                                                                 cTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGc                     <  1:1198735/149‑1 (MQ=255)

                                                                                                                                   aCCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTg                   >  1:3374169/1‑149 (MQ=255)

                                                                                                                                    cctcGGTTATTACTCACAGGACTGGACGAATCTTGATGCCAATATGGGCTACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATTCCACGCTgg                  <  2:4208949/146‑1 (MQ=255)

                                                                                                                                      aCGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCg                >  2:3148472/1‑149 (MQ=255)

                                                                                                                                       cGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCgg               <  2:4058127/149‑1 (MQ=255)

                                                                                                                                            attaCACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATg          <  2:875136/149‑1 (MQ=255)

                                                                                                                                             ttaCACATAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGc         >  2:2301081/1‑149 (MQ=255)

                                                                                                                                             ttaCACATAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGc         >  2:1880009/1‑149 (MQ=255)

                                                                                                                                             ttaCACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGACCCACACCGGCTATGCCACGCTGGCGGATATGc         >  2:1300295/1‑149 (MQ=255)

                                                                                                                                             ttaCACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGc         >  1:3924882/1‑149 (MQ=255)

                                                                                                                                              taCACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCa        >  2:2688989/1‑149 (MQ=255)

                                                                                                                                              taCACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCa        <  1:4462864/149‑1 (MQ=255)

                                                                                                                                               acacacAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCAg       <  1:3148472/149‑1 (MQ=255)

                                                                                                                                               acacacAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCAg       >  1:4297044/1‑149 (MQ=255)

                                                                                                                                               acacacAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCAg       >  1:4360105/1‑149 (MQ=255)

                                                                                                                                                    caGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCAGGAGTa  <  2:5060521/149‑1 (MQ=255)

ACGCGGCCTGACCAACAAACTGGATTACCTCCAGCAGTTGGGCGTTAATGCTTTATGGATAAGCGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTTCCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCAACGAAGCCGATCTACGGACGCTGGTTGATAGCGCACATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGCTATGCCACGCTGGCGGATATGCAGGAGTA  >  CP009273/3731540‑3731836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`