BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,340	A→C	14.6%	Q12H (CAA→CAC)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,340	0	A	C	14.6%	490.1 / 26.7	264	Q12H (CAA→CAC)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (134/86); new base C (8/36); total (142/122)
Fisher's exact test for biased strand distribution p-value = 2.45e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCATCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                      <  2:1778804/149‑1 (MQ=255)

    aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAcccg                                                                                                                                                  >  1:3628356/1‑147 (MQ=255)

     aaaaGTGCATATATCTCATTAAAATATTTAGATGAGGATGTGGCAAACGTTTTATTTTATCTTTAGGTCAGCTAAAATGCCAGCATTGATACAACGTGATTTTTTTCTATGTCTCTTTCCACACACGAGACAGAAAATGCGAAACCgct                                                                                                                                                 >  2:2101708/1‑147 (MQ=255)

      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                <  1:2129424/149‑1 (MQ=255)

       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTg                                                                                                                                               >  2:1347979/1‑149 (MQ=255)

         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                             >  1:2563082/1‑149 (MQ=255)

           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCa                                                                                                                                           >  2:1812561/1‑149 (MQ=255)

            cATTTATCTCATTTAAATATTTAGATGATGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTATGTTTCCACCCTCGAGTCATAAAATGCGCAACCTGTTGCGCAg                                                                                                                                          <  2:2414291/149‑1 (MQ=255)

            cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATTTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcgccg                                                                                                                                          >  2:1172384/1‑147 (MQ=255)

            cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAAAGTTTGCTTTACTCTATCGGTCAGCTAAAATGACCGCTTTGATACCACGGGATTGTTTTCGATGTATGTTTCCACCCTCGAGACAGAAAATGCGCAAACGGTTGCGCAg                                                                                                                                          >  2:5055470/1‑149 (MQ=255)

             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTCGCAACCCGTTGCGCAgc                                                                                                                                         >  1:2627807/1‑148 (MQ=255)

              tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                        <  2:1093158/149‑1 (MQ=255)

                     cATTTAAATATTTAGATGTGGTTGTGGCAAACGTTTGCTTTTCTCTTGCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTGTGTTTCCACCCTCGAGTCAGAAATTGCGCACCCGGTTGCGCAGACTCAAAAc                                                                                                                                 <  2:3482057/149‑1 (MQ=255)

                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCCCAGACACAAAACaa                                                                                                                               >  1:3361871/1‑148 (MQ=255)

                        ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGTTTGCGCAGACTCAAAACAGc                                                                                                                              <  2:3094637/149‑1 (MQ=255)

                         tAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                                                                                                             >  2:825800/1‑149 (MQ=255)

                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTCGCAACCCGTTGAGCAGaaacaaaacaaaga                                                                                                                            >  1:1419803/1‑145 (MQ=255)

                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAACCGGGTGCGCAGAATCAAAAAAGCGa                                                                                                                            >  1:121650/1‑149 (MQ=255)

                           aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAG‑AAATGCGCAAACCGTTTCGCACACAAAAAAAAACGAAc                                                                                                                          >  1:1042436/1‑149 (MQ=255)

                            atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:1634990/149‑1 (MQ=255)

                             tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACt                                                                                                                         >  2:1707217/1‑149 (MQ=255)

                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAACCGAAATGAt                                                                                                                      >  1:3746575/1‑149 (MQ=255)

                                 tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGAtt                                                                                                                     >  2:3564079/1‑149 (MQ=255)

                                  aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                                                                                                                    >  2:2758201/1‑149 (MQ=255)

                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACAGCTTTaa                                                                                                                  >  2:3926754/1‑148 (MQ=255)

                                     tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTGCGCAGACTCAAAAAAGCGAACTGCTTTAcc                                                                                                                 >  1:2827015/1‑149 (MQ=255)

                                      gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGCCTCAAAACAGCGAACTGATTTACCg                                                                                                                >  2:4241668/1‑149 (MQ=255)

                                        ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCACACACAAAAAAAACAACTGAATTaaccta                                                                                                              >  1:4358520/1‑144 (MQ=255)

                                        ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGATGCGCAGACTCAAAACAGCGAACTGATTTACCgac                                                                                                              >  2:4457640/1‑147 (MQ=255)

                                        ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGATAACATGCCCGCTTTGCTACCAGGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                              <  2:2556034/149‑1 (MQ=255)

                                         gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGATTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                             <  2:2799145/149‑1 (MQ=255)

                                          ttgtgGCAAATGTTTGCTTTTCTCTTTCGGTCAGCTAAACTGCCCGCTTTGCTACCTGGGTTTTGTTTTCGTTGTGTGTTTCCACCCTCGAGTCATAAACGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                            <  1:1126871/148‑1 (MQ=255)

                                          ttgtgGCAAACTTTTTCTTTTCTCTTTCGGTCAGCTAAATTCCCCGTTTTGCTACCAGGGTTTTTTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                            <  1:4946602/148‑1 (MQ=255)

                                                 aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                     <  2:407489/149‑1 (MQ=255)

                                                   aCGTTTGCTTTTCTCTTTCGGTCAGCTAAACTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCTAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                   <  2:3595201/149‑1 (MQ=255)

                                                   aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                   >  2:3131770/1‑149 (MQ=255)

                                                     gTTTGGTTTTCTCTTTGGTTCTTATAAATGGCCCGTTTTGCTACCAGGGTTTTGTTTTCGTTGTTTTTTTCCACCCTCGAGTCATAATTTGCGCAAGGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                 <  1:4156669/149‑1 (MQ=255)

                                                     gTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                 >  2:3981154/1‑149 (MQ=255)

                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTccgc                                                                                               >  2:2171952/1‑149 (MQ=255)

                                                          cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCcgccgc                                                                                            >  2:1274004/1‑149 (MQ=255)

                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTAAAAAAAGCGAAAAGATTTAAAGTATTTAATATAGTccgccccatc                                                                                         >  1:3135367/1‑144 (MQ=255)

                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                        <  2:353346/149‑1 (MQ=255)

                                                                tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTc                                                                                      >  2:2086831/1‑149 (MQ=255)

                                                                 ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCa                                                                                     >  2:3536037/1‑149 (MQ=255)

                                                                      ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                >  2:2022204/1‑149 (MQ=255)

                                                                      ggTCAGCTAAAAGGCCCGCTTTGCTACCACGGGTTTGTTTTCTAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                <  1:2942065/149‑1 (MQ=255)

                                                                      gctCAGATAATATGCCCGATTTGCTACCTGGTTTTTGTTTTGTTTTTCTGTTCCCACCCTCGTGTCATAAAATGCGCAACGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                <  1:1558453/147‑1 (MQ=255)

                                                                          aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                            >  2:401237/1‑149 (MQ=255)

                                                                          aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAATATAGTCCGCCGCGGCCTCAAAACCAGtt                                                                            >  1:2291203/1‑149 (MQ=255)

                                                                           gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGTAGATAGTCCGCCGCTTCCTCAAACCCAGttt                                                                           >  1:592471/1‑149 (MQ=255)

                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                     >  2:56291/1‑149 (MQ=255)

                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCACAAACCCTGTTTgccgcc                                                                     >  2:2950132/1‑149 (MQ=255)

                                                                                    cccGATTTGCTACCTCGTTTTTTTTTTCGATGTGTGTCCCCACCCTCTATTAAAAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                                  <  1:3474956/149‑1 (MQ=255)

                                                                                      cGCTGTGCTCCCCCGGGATTGTTTTCGATGTGTGTTTCCCCCCTCGAGTCAGACAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                <  2:906931/149‑1 (MQ=255)

                                                                                       gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                               >  2:3667992/1‑149 (MQ=255)

                                                                                        cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGATTGCCGCCTGTCAGc                                                              >  2:3896618/1‑149 (MQ=255)

                                                                                         tttGCTACCCGGGGTTTGTTTTCGTTGTTTGTTACCCCCCTCGAGTCACAAAAGGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                             <  1:4573547/149‑1 (MQ=255)

                                                                                            gatACCACGGGATTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          <  2:101/147‑1 (MQ=255)

                                                                                             ccaCCCCGGGATTGTTTCGGTTGTCTGTTACCACCCTCGAGACATAACCGGCCCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctg                                                         <  1:4008448/147‑1 (MQ=255)

                                                                                             cTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCAACCGTTGCGCAGACGCAAAACAACGACCAGATTTAACGTCTTTAATATCGTCCGCCCCGTCCACAAACCCAGTTTTCCGCCTGTCAGCAtatt                                                         >  1:4487122/1‑146 (MQ=255)

                                                                                             cTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATctg                                                         >  2:2480378/1‑149 (MQ=255)

                                                                                              tACCCCGGTTTTTTTTTCGTTGTCTGTTTCCACCCCCTAGTCACAAATTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                        <  1:1661973/149‑1 (MQ=255)

                                                                                               aCCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgct                                                       <  2:4855340/149‑1 (MQ=255)

                                                                                                  aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTTCGCAGACTCAAAACAGCGAACTGATTTACCGTCATGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTTGCGCCAGTCAGCAACTGCTGGc                                                    >  1:3210619/1‑149 (MQ=255)

                                                                                                     ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAAAAACCAACAGATTTAAAGTCTTGAATATAGTCCGCCCCATCCTCAAACCCAGGTTGACGCCAGTCACCATCTGCTGGCGAt                                                 >  1:3532351/1‑149 (MQ=255)

                                                                                                      gATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                <  2:387046/149‑1 (MQ=255)

                                                                                                         tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACAGATTTAACGTCTTTTAGATAGTCCGCCGCGTCCTCAAACCCTGTTTTGCGCCAGTAAGCAAATGCTGGCGATGtta                                             >  1:195547/1‑148 (MQ=255)

                                                                                                           ttttgtttGTCTGTTCCCACCCTGGAGTAAGAAAAGGCGCCAGGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           <  1:3944805/142‑1 (MQ=255)

                                                                                                           ttttCTTTGTCTTTTACCACCCTCGAGTCATAAACTGCGCAACCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           <  1:5097656/149‑1 (MQ=255)

                                                                                                           ttttCGTGGTCTGTCCCCACCCTCGACACACAAAATGCGCACCCGGGCCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           <  1:463177/149‑1 (MQ=255)

                                                                                                             ttCGTTGTCTGTTACCCCCCTCGAGTCATAAAAGGCGCACCGGGGGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                         <  1:1364493/149‑1 (MQ=255)

                                                                                                              tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                        >  2:5167934/1‑149 (MQ=255)

                                                                                                               cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                       >  2:3357321/1‑149 (MQ=255)

                                                                                                                gATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACACGGTTGCGCCGACGCAAAAAAGCGAAAAGATTTTACGTCTTGAAGATAGTACGCCGCGACCGCAAACCCAGTTTGCCGCCTGTCAGCATCTGCTGGCGCGGTTCGtttggg                                      >  1:1813002/1‑145 (MQ=255)

                                                                                                                gATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                      <  2:731479/149‑1 (MQ=255)

                                                                                                                 ttGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                     <  2:4751929/148‑1 (MQ=255)

                                                                                                                    tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  >  2:4664388/1‑149 (MQ=255)

                                                                                                                    tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  >  2:3270667/1‑149 (MQ=255)

                                                                                                                    tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                  >  2:669985/1‑149 (MQ=255)

                                                                                                                    tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGGTGCGGTGAt                                  >  2:1918177/1‑149 (MQ=255)

                                                                                                                      tttttCCCCCCTCGAGACATAAATTGCCCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  1:630362/147‑1 (MQ=255)

                                                                                                                            cACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGGCTTGAAGATCGTCCGCCCCTTCCTCAAACCCGGTTTGCCGCCGGTCAGCCACTGCTGGCGATGTTCGTTGCGGTGATCACGcccc                          >  1:3013136/1‑147 (MQ=255)

                                                                                                                             aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                         <  2:4954423/149‑1 (MQ=255)

                                                                                                                             aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGGCTTTAATATCGTCCGCCGCTTCCTCAAACCCAGTTTGGCGCCTGTCAGCAACTTCTGGCGATGTTCGTTGCGGTGATAACGCCAgc                         >  1:3883764/1‑149 (MQ=255)

                                                                                                                                 tCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACACAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCCCCGCGGCCTCAAACCCTGTTTGCCGCCAGTCAGCAACTGCTGGCGATGTTCGTTGCGGTTATCACGCCAGCGCTa                     >  1:1679468/1‑149 (MQ=255)

                                                                                                                                 tCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTGAAGATCGTCCCCCGCTTCCTCAAACCCAGGTTTCCGCCTGTAAGCATCAGCTGGCGTTGTTCGGTGCGGTGTTCACGCCCGCGCTa                     >  1:1680018/1‑149 (MQ=255)

                                                                                                                                  ggcgTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAt                    <  2:2897531/146‑1 (MQ=255)

                                                                                                                                   gAGTCAGAAAATGCGCAACCGGTTGCACAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  2:155187/149‑1 (MQ=255)

                                                                                                                                    aGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCGATTa                  >  2:2325089/1‑149 (MQ=255)

                                                                                                                                     gTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa                 <  2:3013136/149‑1 (MQ=255)

                                                                                                                                      tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                <  2:199436/149‑1 (MQ=255)

                                                                                                                                       aacaaaaTGCGCAACCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc               <  1:2444273/146‑1 (MQ=255)

                                                                                                                                        aGACAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  2:391268/149‑1 (MQ=255)

                                                                                                                                           aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           >  1:498304/1‑149 (MQ=255)

                                                                                                                                           aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc           >  1:924939/1‑149 (MQ=255)

                                                                                                                                            aaTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  2:3063371/149‑1 (MQ=255)

                                                                                                                                            aaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          >  1:4874736/1‑149 (MQ=255)

                                                                                                                                                  cAACGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt    <  1:3321361/149‑1 (MQ=255)

                                                                                                                                                    aCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  2:4417560/1‑149 (MQ=255)

                                                                                                                                                    aCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  1:2902492/1‑149 (MQ=255)

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`