BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,921,086	A→T	7.3%	K191I (AAA→ATA)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,921,086	0	A	T	7.3%	1064.5 / 58.0	300	K191I (AAA→ATA)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base A (147/131); new base T (12/10); total (159/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 307 total aligned reads displayed.

CGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAAC  >  CP009273/3920939‑3921234

 gggAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAg                                                                                                                                                    <  1:4654081/149‑1 (MQ=255)

   gAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGAt                                                                                                                                                  <  2:2156383/149‑1 (MQ=255)

   gAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGAt                                                                                                                                                  <  1:2575850/149‑1 (MQ=255)

    aaTTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATc                                                                                                                                                 >  1:554489/1‑149 (MQ=255)

    aaTTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATc                                                                                                                                                 >  2:1113051/1‑149 (MQ=255)

    aaTTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATc                                                                                                                                                 >  1:161493/1‑149 (MQ=255)

    aaTTAAAGCAACCGAAGCTGCGGTGAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATc                                                                                                                                                 <  2:4954956/149‑1 (MQ=255)

     aTTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCt                                                                                                                                                >  1:785108/1‑149 (MQ=255)

        aaaGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgg                                                                                                                                             <  1:4325011/149‑1 (MQ=255)

         aaGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGgc                                                                                                                                            >  1:2921355/1‑149 (MQ=255)

          aGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGgcg                                                                                                                                           >  1:3628535/1‑149 (MQ=255)

             aaCCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCgg                                                                                                                                        >  2:4492349/1‑149 (MQ=255)

               ccGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTa                                                                                                                                      <  2:2882566/149‑1 (MQ=255)

                 gAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTAtt                                                                                                                                    >  1:1774106/1‑149 (MQ=255)

                  aaGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTc                                                                                                                                   >  1:1483355/1‑149 (MQ=255)

                   aGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTcc                                                                                                                                  >  2:4209821/1‑149 (MQ=255)

                   aGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTcc                                                                                                                                  >  2:288533/1‑149 (MQ=255)

                    gCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCt                                                                                                                                 >  1:3531702/1‑149 (MQ=255)

                      tGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTg                                                                                                                               >  1:4580661/1‑149 (MQ=255)

                      tGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTg                                                                                                                               >  2:2072201/1‑149 (MQ=255)

                      tGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTg                                                                                                                               <  1:136068/149‑1 (MQ=255)

                             aGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGAt                                                                                                                        >  2:2640805/1‑149 (MQ=255)

                              gCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGAtt                                                                                                                       >  1:2536529/1‑149 (MQ=255)

                                gAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTgg                                                                                                                     <  2:4684710/149‑1 (MQ=255)

                                 aaGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggc                                                                                                                    <  1:3560232/149‑1 (MQ=255)

                                  agagTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcg                                                                                                                   <  2:507625/149‑1 (MQ=255)

                                  agagTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcg                                                                                                                   >  1:4142450/1‑149 (MQ=255)

                                    agTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcggc                                                                                                                 <  2:2935925/149‑1 (MQ=255)

                                     gTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCa                                                                                                                <  1:806444/149‑1 (MQ=255)

                                      tttGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCaa                                                                                                               >  2:3171040/1‑149 (MQ=255)

                                      tttGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCaa                                                                                                               <  1:1546737/149‑1 (MQ=255)

                                      tttGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCaa                                                                                                               >  2:4978497/1‑149 (MQ=255)

                                          gCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCACGCTg                                                                                                           >  1:4495560/1‑149 (MQ=255)

                                          gCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTg                                                                                                           >  1:5055753/1‑149 (MQ=255)

                                          gCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTg                                                                                                           <  1:4122236/149‑1 (MQ=255)

                                           ccTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGa                                                                                                          <  2:3281923/149‑1 (MQ=255)

                                            cTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAg                                                                                                         <  1:1303213/149‑1 (MQ=255)

                                             tGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                                                                        >  1:3782225/1‑149 (MQ=255)

                                              ggCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCg                                                                                                       <  2:4100459/149‑1 (MQ=255)

                                              ggCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCg                                                                                                       >  1:3040135/1‑149 (MQ=255)

                                               gCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTCTTCCTTGTCGGGATTGGCGGCAAGCTGAGCGa                                                                                                      >  2:1834211/1‑149 (MQ=255)

                                               gCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGa                                                                                                      >  2:4428441/1‑149 (MQ=255)

                                                cACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGAt                                                                                                     >  2:4759283/1‑149 (MQ=255)

                                                  ccGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                                                                   <  1:674715/149‑1 (MQ=255)

                                                    gTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTc                                                                                                 <  2:1968043/149‑1 (MQ=255)

                                                      tCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCAt                                                                                               <  2:1649322/149‑1 (MQ=255)

                                                       ccTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATc                                                                                              <  2:3058427/149‑1 (MQ=255)

                                                        cTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCg                                                                                             >  2:5034230/1‑149 (MQ=255)

                                                         tGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGc                                                                                            >  1:1079587/1‑149 (MQ=255)

                                                             ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacg                                                                                        >  1:3926285/1‑149 (MQ=255)

                                                              gATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGATAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacga                                                                                       <  2:1468880/149‑1 (MQ=255)

                                                              gATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacga                                                                                       <  2:880705/149‑1 (MQ=255)

                                                              gATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacga                                                                                       >  1:4979917/1‑149 (MQ=255)

                                                              gATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacga                                                                                       >  1:2093775/1‑149 (MQ=255)

                                                               aTCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgac                                                                                      >  1:2430511/1‑149 (MQ=255)

                                                                tCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGATAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgacg                                                                                     <  2:4909174/149‑1 (MQ=255)

                                                                  aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTg                                                                                   >  1:74844/1‑149 (MQ=255)

                                                                  aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTg                                                                                   >  1:4860843/1‑149 (MQ=255)

                                                                     tCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgc                                                                                <  1:3204014/149‑1 (MQ=255)

                                                                       cACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgcgc                                                                              >  2:3251942/1‑149 (MQ=255)

                                                                        aCTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCa                                                                             >  1:2651246/1‑149 (MQ=255)

                                                                        aCTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCa                                                                             >  1:4044941/1‑149 (MQ=255)

                                                                             gTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGa                                                                        <  1:1909363/149‑1 (MQ=255)

                                                                              tACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAt                                                                       <  2:917692/149‑1 (MQ=255)

                                                                              tACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAt                                                                       <  1:518237/149‑1 (MQ=255)

                                                                               acacAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAtt                                                                      <  2:1507420/149‑1 (MQ=255)

                                                                                   aGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatga                                                                  >  2:3890570/1‑149 (MQ=255)

                                                                                    gCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                                                                 >  2:2837253/1‑149 (MQ=255)

                                                                                     ccAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgatg                                                                >  1:904714/1‑149 (MQ=255)

                                                                                     ccAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgatg                                                                <  1:2027111/149‑1 (MQ=255)

                                                                                     ccAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGACGGTCATCGCCACGACGTGCGCGCACCGGATTatgatg                                                                <  2:3571750/149‑1 (MQ=255)

                                                                                         gAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTg                                                            <  2:2114253/149‑1 (MQ=255)

                                                                                           gTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCCCCGGATTATGATGACTGGa                                                          >  2:793390/1‑149 (MQ=255)

                                                                                             tACTGTCTCGTTATCCGGATCTTGCTGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGc                                                        <  2:3751384/149‑1 (MQ=255)

                                                                                              aCTGTCTCGTTATCCGGATCTTAATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCa                                                       >  1:980711/1‑149 (MQ=255)

                                                                                                  tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcccc                                                   <  1:2684072/149‑1 (MQ=255)

                                                                                                    tcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGt                                                 >  1:3705998/1‑149 (MQ=255)

                                                                                                     cGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTc                                                <  2:2921355/149‑1 (MQ=255)

                                                                                                      gTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCa                                               >  1:1870635/1‑149 (MQ=255)

                                                                                                      gTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCa                                               >  1:3880870/1‑149 (MQ=255)

                                                                                                       ttATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCag                                              <  2:5123957/149‑1 (MQ=255)

                                                                                                       ttATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCag                                              >  1:1004143/1‑149 (MQ=255)

                                                                                                        tATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCaga                                             >  1:474351/1‑149 (MQ=255)

                                                                                                         aTCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCagag                                            >  2:2572538/1‑149 (MQ=255)

                                                                                                            cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTg                                         >  2:5103730/1‑149 (MQ=255)

                                                                                                                        ccAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGt                             <  2:4587096/149‑1 (MQ=255)

                                                                                                                         cAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTc                            >  1:2794483/1‑149 (MQ=255)

                                                                                                                          aaaGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCt                           >  2:1376327/1‑149 (MQ=255)

                                                                                                                            aGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGa                         <  2:272680/149‑1 (MQ=255)

                                                                                                                             gggCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCCCCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGaa                        >  1:3296964/1‑149 (MQ=255)

                                                                                                                             gggCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGaa                        >  1:2260545/1‑149 (MQ=255)

                                                                                                                                 gTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGc                    <  2:1216550/149‑1 (MQ=255)

                                                                                                                                   gAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGa                  >  2:2233316/1‑149 (MQ=255)

                                                                                                                                     gCGGGCTATAGAGAAAGATATTGGCGCGTTATTCGTTGTCGGTTTTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTTAACGGCGata                <  1:2310424/149‑1 (MQ=255)

                                                                                                                                     gCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGata                >  1:3098033/1‑149 (MQ=255)

                                                                                                                                         gCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCt            <  2:761516/149‑1 (MQ=255)

                                                                                                                                           gATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTgg          <  2:740069/149‑1 (MQ=255)

                                                                                                                                             tAGCGAAAGATCTTGGCGCGGTATCCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGgtg        >  1:46306/1‑149 (MQ=255)

                                                                                                                                                cGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTgg     <  2:2430511/149‑1 (MQ=255)

                                                                                                                                                  aaaGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGaa   <  1:4903774/149‑1 (MQ=255)

CGGGAATTAAAGCAACCGAAGCTGCGGTTAGCGAAGAGTTTGGCCTGGCACCGTTCCTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAAC  >  CP009273/3920939‑3921234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`