Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 29,139 G→T 25.4% G256C (GGT→TGT)  dapB → dihydrodipicolinate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927329,1390GT25.4% 663.1 / 206.7 282G256C (GGT→TGT) dapBdihydrodipicolinate reductase
Reads supporting (aligned to +/- strand):  ref base G (88/122);  new base T (27/45);  total (115/167)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

TGGTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGTT  >  CP009273/28991‑29285
                                                                                                                                                    |                                                                                                                                                  
  gTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGt                                                                                                                                                  <  1:600843/149‑1 (MQ=255)
       gCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGa                                                                                                                                             >  2:4521144/1‑149 (MQ=255)
         cACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGaaa                                                                                                                                           <  2:2326254/149‑1 (MQ=255)
          aCCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAg                                                                                                                                          <  2:4115245/149‑1 (MQ=255)
             gtgcgtgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCgg                                                                                                                                       >  2:540815/1‑149 (MQ=255)
                  tgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCttt                                                                                                                                  <  1:3752671/149‑1 (MQ=255)
                  tgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCttt                                                                                                                                  <  2:3285547/149‑1 (MQ=255)
                  tgcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCttt                                                                                                                                  >  2:1082337/1‑149 (MQ=255)
                   gcAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCtttt                                                                                                                                 <  2:3209493/149‑1 (MQ=255)
                      ggTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGa                                                                                                                              >  1:1213712/1‑149 (MQ=255)
                       gTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGat                                                                                                                             <  1:4880623/149‑1 (MQ=255)
                       gTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGat                                                                                                                             <  1:1292514/149‑1 (MQ=255)
                       gTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGat                                                                                                                             >  1:4168593/1‑149 (MQ=255)
                        tGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGata                                                                                                                            <  1:4780760/149‑1 (MQ=255)
                         gACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGatat                                                                                                                           <  1:3024747/149‑1 (MQ=255)
                         gACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGatat                                                                                                                           >  2:1152299/1‑149 (MQ=255)
                               gTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCgaga                                                                                                                     <  2:388350/149‑1 (MQ=255)
                                  ggTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGt                                                                                                                  >  1:866188/1‑149 (MQ=255)
                                   gTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTa                                                                                                                 >  2:2995266/1‑149 (MQ=255)
                                   gTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTa                                                                                                                 >  1:3187078/1‑149 (MQ=255)
                                      aaCATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACtt                                                                                                              <  2:3916430/149‑1 (MQ=255)
                                      aaCATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACtt                                                                                                              <  2:4871738/149‑1 (MQ=255)
                                      aaCATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACtt                                                                                                              >  1:768663/1‑149 (MQ=255)
                                       aCATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTg                                                                                                             >  2:518685/1‑149 (MQ=255)
                                       aCATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTg                                                                                                             >  1:758541/1‑149 (MQ=255)
                                         aTACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGAt                                                                                                           <  2:3863587/149‑1 (MQ=255)
                                         aTACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGAt                                                                                                           >  2:3500495/1‑149 (MQ=255)
                                           aCCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGAtct                                                                                                         >  2:3185579/1‑149 (MQ=255)
                                              gcgATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaa                                                                                                      <  1:5091628/149‑1 (MQ=255)
                                                gATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCaata                                                                                                    >  1:1325617/1‑149 (MQ=255)
                                                    tttGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAAttt                                                                                                >  1:1636940/1‑149 (MQ=255)
                                                     ttGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTg                                                                                               <  1:2097200/149‑1 (MQ=255)
                                                       gCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTa                                                                                             <  1:317062/149‑1 (MQ=255)
                                                          gATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTTTGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAAcc                                                                                          <  1:23973/149‑1 (MQ=255)
                                                          gATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAAcc                                                                                          >  2:1418948/1‑149 (MQ=255)
                                                             atTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTCAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACcaca                                                                                       >  2:3254024/1‑149 (MQ=255)
                                                             atTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACcaca                                                                                       <  2:1385159/149‑1 (MQ=255)
                                                                ggCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACaaaa                                                                                    <  1:1859732/149‑1 (MQ=255)
                                                                ggCGAGCGTCTGAAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACaaaa                                                                                    >  1:145983/1‑149 (MQ=255)
                                                                   gAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAatat                                                                                 <  2:2658799/149‑1 (MQ=255)
                                                                   gAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAatat                                                                                 >  1:1031676/1‑149 (MQ=255)
                                                                   gAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAatat                                                                                 <  1:3858153/149‑1 (MQ=255)
                                                                   gAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAatat                                                                                 <  2:3619470/149‑1 (MQ=255)
                                                                   gAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAatat                                                                                 <  1:1808545/149‑1 (MQ=255)
                                                                    aGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATAtt                                                                                <  1:3078427/149‑1 (MQ=255)
                                                                        tCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGtt                                                                            <  1:3648579/149‑1 (MQ=255)
                                                                        tCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGtt                                                                            <  2:4013849/149‑1 (MQ=255)
                                                                        tCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGtt                                                                            <  1:1969847/149‑1 (MQ=255)
                                                                           ggAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATg                                                                         >  1:967040/1‑149 (MQ=255)
                                                                            gagaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATgg                                                                        <  2:3923897/149‑1 (MQ=255)
                                                                            gagaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCT‑ACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGt                                                                       <  2:2903503/149‑1 (MQ=255)
                                                                             agaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTCACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGt                                                                       <  2:5018292/149‑1 (MQ=255)
                                                                             agaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGt                                                                       <  1:1082337/149‑1 (MQ=255)
                                                                              gaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTg                                                                      >  2:4677517/1‑149 (MQ=255)
                                                                              gaTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTg                                                                      >  2:4959855/1‑149 (MQ=255)
                                                                               aTCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGc                                                                     <  2:788293/149‑1 (MQ=255)
                                                                               aTCACCCATAAGGCGTCCAGCAGTATGACATTTGCTAACGGCGAGGTAATATCGTCTTTGTGGTTGATTTGTAAGGAAAGCGTTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGc                                                                     <  2:2260106/149‑1 (MQ=255)
                                                                                   cccATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCaaaa                                                                 >  1:557194/1‑149 (MQ=255)
                                                                                          ggCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAacac                                                          <  2:3728468/149‑1 (MQ=255)
                                                                                            cGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACat                                                        <  1:4307767/149‑1 (MQ=255)
                                                                                             gTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatt                                                       >  1:2907234/1‑149 (MQ=255)
                                                                                             gTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatt                                                       <  1:453088/149‑1 (MQ=255)
                                                                                                cAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaa                                                    <  2:4765181/149‑1 (MQ=255)
                                                                                                cAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaa                                                    >  1:297429/1‑149 (MQ=255)
                                                                                                  gCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaatt                                                  >  1:2682208/1‑149 (MQ=255)
                                                                                                    cGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACatttaattta                                                <  1:1588537/149‑1 (MQ=255)
                                                                                                     gTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTAt                                               <  2:3411496/149‑1 (MQ=255)
                                                                                                      tATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTAtt                                              <  2:305538/149‑1 (MQ=255)
                                                                                                      tATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCAC‑AAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTg                                             >  1:4417453/1‑149 (MQ=255)
                                                                                                          aCATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGAtt                                          <  2:1627550/149‑1 (MQ=255)
                                                                                                           cATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATta                                         >  2:2855311/1‑149 (MQ=255)
                                                                                                            aTTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATtat                                        >  1:166820/1‑149 (MQ=255)
                                                                                                                gCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAg                                    <  1:654076/149‑1 (MQ=255)
                                                                                                                    aCGGCGCGGTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCt                                >  1:1536991/1‑149 (MQ=255)
                                                                                                                    aCGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCt                                >  1:1209550/1‑149 (MQ=255)
                                                                                                                     cGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCtt                               <  1:2147806/149‑1 (MQ=255)
                                                                                                                      ggCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCttt                              <  2:4264853/149‑1 (MQ=255)
                                                                                                                        cgcgGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTaa                            <  2:2900524/149‑1 (MQ=255)
                                                                                                                         gcgGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAt                           <  2:2045907/149‑1 (MQ=255)
                                                                                                                          cgGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAtt                          <  1:2781498/149‑1 (MQ=255)
                                                                                                                            gTAATATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAtttt                        <  1:3373468/149‑1 (MQ=255)
                                                                                                                            gTAAGATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAtttt                        >  1:4529088/1‑149 (MQ=255)
                                                                                                                             tAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTTTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTAATTGAATATAAAGTGCTTTAAttttt                       >  1:921600/1‑149 (MQ=255)
                                                                                                                             tAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAAttttt                       >  1:658012/1‑149 (MQ=255)
                                                                                                                               aGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTgg                     <  2:601879/149‑1 (MQ=255)
                                                                                                                                gATCGGCTTTGTGGTTGAGTTGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGc                    <  1:245257/149‑1 (MQ=255)
                                                                                                                                gATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTgga                    >  2:1841073/1‑148 (MQ=255)
                                                                                                                                gATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGc                    >  1:3230545/1‑149 (MQ=255)
                                                                                                                                   cGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCt                 <  1:175900/149‑1 (MQ=255)
                                                                                                                                   cGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCt                 <  1:2874228/149‑1 (MQ=255)
                                                                                                                                   cGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCt                 <  2:2517858/149‑1 (MQ=255)
                                                                                                                                   cGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCt                 <  1:4855973/149‑1 (MQ=255)
                                                                                                                                       tttGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGTCCCTTTTa             >  2:512649/1‑149 (MQ=255)
                                                                                                                                       tttGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACAACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATCAAGGGCTTTAATTTTTGGCCCTTTTa             >  2:2725373/1‑149 (MQ=255)
                                                                                                                                           tgGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTAtttt         <  1:4724086/149‑1 (MQ=255)
                                                                                                                                             gTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTg       >  1:353530/1‑149 (MQ=255)
                                                                                                                                             gTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTg       >  1:2583232/1‑149 (MQ=255)
                                                                                                                                              ttGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTgg      >  1:4271001/1‑149 (MQ=255)
                                                                                                                                              ttGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTgg      <  2:1593566/149‑1 (MQ=255)
                                                                                                                                                 aGTGGTAAGGACAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGgtgt   <  2:1063151/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACATACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGTATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAACCACAAAATATTTGTTATGGTGCAAAAATAACACATTTAATTTATTGATTATAAAGGGCTTTAATTTTTGGCCCTTTTATTTTTGGTGTT  >  CP009273/28991‑29285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑