BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,164,396	C→A	6.0%	D187Y (GAT→TAT)	coaA ←	pantothenate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,164,396	0	C	A	6.0%	1311.7 / 42.5	339	D187Y (GAT→TAT)	coaA	pantothenate kinase
Reads supporting (aligned to +/- strand): ref base C (182/136); new base A (11/10); total (193/146)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

AGTAAGTCTTCCGGTGCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAGACCACGTTCT  >  CP009273/4164248‑4164544

agtaagtCTTCCGGTGCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTAtc                                                                                                                                                      <  2:3237614/149‑1 (MQ=255)

    agtCTTCCGGTGCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCa                                                                                                                                                  >  2:1442152/1‑149 (MQ=255)

          ccGGTGCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCggg                                                                                                                                            >  2:1186800/1‑149 (MQ=255)

           cGGTGCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGa                                                                                                                                           <  1:635064/149‑1 (MQ=255)

             gTGCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATc                                                                                                                                         <  2:24890/149‑1 (MQ=255)

               gCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATcac                                                                                                                                       >  1:3700284/1‑149 (MQ=255)

                  tCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCAcatc                                                                                                                                    <  1:224390/149‑1 (MQ=255)

                    aaCATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCAcatcat                                                                                                                                  >  2:3574268/1‑149 (MQ=255)

                     aCATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCata                                                                                                                                 <  1:1711119/149‑1 (MQ=255)

                      cATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCataa                                                                                                                                >  1:4101571/1‑149 (MQ=255)

                        tatatCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCataaat                                                                                                                              <  2:3118716/149‑1 (MQ=255)

                        tatatCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCataaat                                                                                                                              >  2:166396/1‑149 (MQ=255)

                             cGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGAt                                                                                                                         <  2:1768252/149‑1 (MQ=255)

                               aaaaaTCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGAtgt                                                                                                                       >  2:184632/1‑149 (MQ=255)

                                aaaaTCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGAtgtg                                                                                                                      >  1:835539/1‑149 (MQ=255)

                                  aaTCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAg                                                                                                                    >  1:4883439/1‑149 (MQ=255)

                                   aTCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGt                                                                                                                   >  2:1497643/1‑149 (MQ=255)

                                     cGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTaa                                                                                                                 >  1:3876790/1‑149 (MQ=255)

                                     cGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGAGGTGAGTaa                                                                                                                 >  2:2139771/1‑149 (MQ=255)

                                        cAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACa                                                                                                              >  1:604131/1‑149 (MQ=255)

                                         aaaaTCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAg                                                                                                             <  2:2663038/149‑1 (MQ=255)

                                         aaaaTCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAg                                                                                                             >  1:1915901/1‑149 (MQ=255)

                                          aaaTCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATATCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAgg                                                                                                            <  1:1265222/149‑1 (MQ=255)

                                           aaTCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGt                                                                                                           >  2:2773105/1‑149 (MQ=255)

                                            aTCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTg                                                                                                          >  1:3250557/1‑149 (MQ=255)

                                               aGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTg                                                                                                       <  2:1197592/149‑1 (MQ=255)

                                                  aaCAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTaa                                                                                                    >  2:3322242/1‑149 (MQ=255)

                                                  aaCAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTaa                                                                                                    >  1:2693411/1‑149 (MQ=255)

                                                   aCAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAAc                                                                                                   >  2:846356/1‑149 (MQ=255)

                                                     aaaTACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAATCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGt                                                                                                 >  1:2136584/1‑149 (MQ=255)

                                                       aTACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGttt                                                                                               <  1:632735/149‑1 (MQ=255)

                                                        tACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTg                                                                                              <  1:33299/149‑1 (MQ=255)

                                                        tACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTg                                                                                              >  2:3078765/1‑149 (MQ=255)

                                                          cATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGc                                                                                            >  1:3783258/1‑149 (MQ=255)

                                                           atgatgTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCa                                                                                           >  1:2309072/1‑149 (MQ=255)

                                                            tgatgTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCAc                                                                                          >  2:1427941/1‑149 (MQ=255)

                                                                gtgGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCg                                                                                      <  2:5168277/149‑1 (MQ=255)

                                                                   gATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGAt                                                                                   >  1:2368511/1‑149 (MQ=255)

                                                                   gATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGAt                                                                                   >  1:4299656/1‑149 (MQ=255)

                                                                       gtgtGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCGGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTga                                                                               >  2:4021679/1‑149 (MQ=255)

                                                                         gtgGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTgaga                                                                             <  2:612040/149‑1 (MQ=255)

                                                                         gtgGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTgaga                                                                             >  1:1788957/1‑149 (MQ=255)

                                                                         gtgGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTgaga                                                                             <  1:11752/149‑1 (MQ=255)

                                                                            gATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCg                                                                          >  1:4548902/1‑149 (MQ=255)

                                                                                atccatccCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGaaa                                                                      <  1:1521607/149‑1 (MQ=255)

                                                                                     tccCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGaaacaaac                                                                 >  1:4722258/1‑149 (MQ=255)

                                                                                        cGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTc                                                              <  1:420/149‑1 (MQ=255)

                                                                                         gCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCa                                                             <  1:3469798/149‑1 (MQ=255)

                                                                                            ctGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCa                                                          <  2:2412452/149‑1 (MQ=255)

                                                                                             tGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAg                                                         <  2:4667589/149‑1 (MQ=255)

                                                                                             tGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAg                                                         >  2:1331493/1‑149 (MQ=255)

                                                                                                 aGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGa                                                     >  2:4738477/1‑149 (MQ=255)

                                                                                                 aGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGa                                                     <  2:2243670/149‑1 (MQ=255)

                                                                                                  gACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGAt                                                    >  1:2501839/1‑149 (MQ=255)

                                                                                                     aTTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCa                                                 >  1:4620899/1‑149 (MQ=255)

                                                                                                     aTTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCa                                                 >  1:1991357/1‑149 (MQ=255)

                                                                                                      tttAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCat                                                >  1:285654/1‑149 (MQ=255)

                                                                                                       ttAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCata                                               <  2:1263622/149‑1 (MQ=255)

                                                                                                       ttAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCata                                               <  1:4269036/149‑1 (MQ=255)

                                                                                                          aCCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCa                                            <  2:190238/149‑1 (MQ=255)

                                                                                                           cccTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCAt                                           <  2:4295382/149‑1 (MQ=255)

                                                                                                            ccTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATa                                          <  2:4849259/149‑1 (MQ=255)

                                                                                                                 aaGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGAtt                                     >  2:4078830/1‑149 (MQ=255)

                                                                                                                  aGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTc                                    >  2:4091565/1‑149 (MQ=255)

                                                                                                                  aGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTc                                    >  1:2564634/1‑149 (MQ=255)

                                                                                                                  aGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTc                                    >  2:2529545/1‑149 (MQ=255)

                                                                                                                  aGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTc                                    >  1:5166238/1‑149 (MQ=255)

                                                                                                                   gAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTcc                                   >  1:3077182/1‑149 (MQ=255)

                                                                                                                   gAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTcc                                   >  2:3998557/1‑149 (MQ=255)

                                                                                                                   gAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTcc                                   >  1:2897575/1‑149 (MQ=255)

                                                                                                                   gAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTcc                                   >  2:3106181/1‑149 (MQ=255)

                                                                                                                    aaTTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCg                                  <  2:4219049/149‑1 (MQ=255)

                                                                                                                     aTTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCgg                                 >  2:1316700/1‑149 (MQ=255)

                                                                                                                     aTTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCgg                                 <  2:2549862/149‑1 (MQ=255)

                                                                                                                     aTTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCgg                                 <  2:506424/149‑1 (MQ=255)

                                                                                                                       tAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGa                               <  1:3163834/149‑1 (MQ=255)

                                                                                                                         aaaTATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAg                             >  1:1688382/1‑149 (MQ=255)

                                                                                                                          aaTATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGc                            <  1:464510/149‑1 (MQ=255)

                                                                                                                             atCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCtt                         <  1:917123/149‑1 (MQ=255)

                                                                                                                             atCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCtt                         <  2:1520529/149‑1 (MQ=255)

                                                                                                                             atCAGGCTGAACAACCGTTTTATATCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCtt                         >  2:4796585/1‑149 (MQ=255)

                                                                                                                              tCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCttt                        <  1:3574268/149‑1 (MQ=255)

                                                                                                                                aGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttct                      <  2:2996526/149‑1 (MQ=255)

                                                                                                                                  gCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttc                    <  2:4830626/149‑1 (MQ=255)

                                                                                                                                  gCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttc                    >  2:2114838/1‑149 (MQ=255)

                                                                                                                                  gCTGAACAACCGTTTTATCTCCATCCGCGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAACCAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttc                    >  1:2682542/1‑149 (MQ=255)

                                                                                                                                   cTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttct                   <  2:4545452/149‑1 (MQ=255)

                                                                                                                                    tGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttctt                  >  1:1506157/1‑149 (MQ=255)

                                                                                                                                    tGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttctt                  >  2:2025955/1‑149 (MQ=255)

                                                                                                                                    tGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttctt                  >  1:3929227/1‑149 (MQ=255)

                                                                                                                                    tGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttctt                  <  1:1166199/149‑1 (MQ=255)

                                                                                                                                     gAACAACCGTTTTATCTCCATCCGGGATCACATCCTAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTttcttcttc                 >  1:1842759/1‑149 (MQ=255)

                                                                                                                                      aacaacCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTtca                >  2:1582604/1‑149 (MQ=255)

                                                                                                                                          acCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAg            >  2:3588706/1‑149 (MQ=255)

                                                                                                                                          acCGTTTTATATCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAg            >  2:748084/1‑149 (MQ=255)

                                                                                                                                            cGTTTTATATCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAGAc          >  2:4503725/1‑149 (MQ=255)

                                                                                                                                                 tATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAGACCACGt     >  1:1373261/1‑149 (MQ=255)

                                                                                                                                                   tctcCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAGACCACGTTc   >  1:4781414/1‑149 (MQ=255)

                                                                                                                                                   tctcCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGCGAAGCCTTTCTTATTCATCAGACCACGtta   >  1:2986531/1‑148 (MQ=255)

                                                                                                                                                   tctcAATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAGACCACGTTc   >  2:3763424/1‑149 (MQ=255)

AGTAAGTCTTCCGGTGCATCAACATATATCGAAAAATCGACAAAATCAGAAACAAATACATGATGTGGATCGTGTGGATAATCCATCCCGCTCTGTAAGACATTTAACCCTTCAAGAATTAAAATATCAGGCTGAACAACCGTTTTATCTCCATCCGGGATCACATCATAAATAAGATGTGAGTAAACAGGTGCTGTAACGTTTGGCACGCCGGATTTGAGATCGGAAACAAACTTCACCAGGCGATGCATATCATACGATTCCGGGAAGCCTTTCTTCTTCATCAGACCACGTTCT  >  CP009273/4164248‑4164544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`