BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,212,014	G→T	7.0%	F134L (TTC→TTA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,212,014	0	G	T	7.0%	961.9 / 35.0	252	F134L (TTC→TTA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (90/143); new base T (8/11); total (98/154)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

TTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTAT  >  CP009273/4211866‑4212160

tttCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTggg                                                                                                                                                    <  1:2527263/149‑1 (MQ=255)

  tCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGaa                                                                                                                                                  <  2:3578225/149‑1 (MQ=255)

   cAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAAt                                                                                                                                                 >  1:3290172/1‑149 (MQ=255)

   cAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAt                                                                                                                                                 <  1:1423187/149‑1 (MQ=255)

   cAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAt                                                                                                                                                 <  2:4510748/149‑1 (MQ=255)

     gCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttc                                                                                                                                               >  2:4704140/1‑149 (MQ=255)

       tGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctt                                                                                                                                             <  1:812727/149‑1 (MQ=255)

       tGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctt                                                                                                                                             <  1:1339706/149‑1 (MQ=255)

       tGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctt                                                                                                                                             <  1:4905105/149‑1 (MQ=255)

       tGAAGAGTAATCATCTTGATGGGGTNTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGNAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctt                                                                                                                                             <  1:1853340/149‑1 (MQ=255)

        gAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttcttt                                                                                                                                            >  2:62914/1‑149 (MQ=255)

         aaGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttt                                                                                                                                           <  1:4622554/149‑1 (MQ=255)

         aaGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttt                                                                                                                                           <  1:3993218/149‑1 (MQ=255)

          agagTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttcttttt                                                                                                                                          <  1:2677200/149‑1 (MQ=255)

           gagTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttttc                                                                                                                                         >  2:1853192/1‑149 (MQ=255)

             ggaaTCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttttctt                                                                                                                                       <  2:494305/147‑1 (MQ=255)

             gTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAAttctttttctt                                                                                                                                       >  2:4175974/1‑149 (MQ=255)

             gTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttttctt                                                                                                                                       >  1:1592837/1‑149 (MQ=255)

             gTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAAttctttttctt                                                                                                                                       <  2:2685744/149‑1 (MQ=255)

                   atcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTAt                                                                                                                                 >  1:1756830/1‑149 (MQ=255)

                    tcTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGATTAATTCTTGGGAATTCTTTTTCTTTTCTATc                                                                                                                                >  2:3163225/1‑149 (MQ=255)

                     cTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCt                                                                                                                               <  2:262616/149‑1 (MQ=255)

                     cTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCt                                                                                                                               <  1:429719/149‑1 (MQ=255)

                       tGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGa                                                                                                                             <  2:4134047/149‑1 (MQ=255)

                       tGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGa                                                                                                                             <  2:2515740/149‑1 (MQ=255)

                       tGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGa                                                                                                                             <  1:3640008/149‑1 (MQ=255)

                        gATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTATCTGAt                                                                                                                            <  1:3423926/149‑1 (MQ=255)

                          tGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGAttt                                                                                                                          <  2:3390032/149‑1 (MQ=255)

                           ggggTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGAtttt                                                                                                                         >  1:2747427/1‑149 (MQ=255)

                            gggTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTa                                                                                                                        <  1:2931971/149‑1 (MQ=255)

                             ggTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAg                                                                                                                       >  1:3069448/1‑149 (MQ=255)

                             ggTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAg                                                                                                                       >  1:468229/1‑149 (MQ=255)

                              gTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAgg                                                                                                                      <  2:2501030/149‑1 (MQ=255)

                                   tAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTaa                                                                                                                 >  1:1458364/1‑149 (MQ=255)

                                    aaTCAACATTCTTATAAATATCATTAATATATTGTTCATCAATTTGTAGTCATAATATACGGATTTGTGGTCTCCCAGTAATATATCATTGAACATTATGTTTAATTTGTTTGAATTATTTTTCTTTTCTATCTGATTTTAGGTTTAAt                                                                                                                <  2:652170/149‑1 (MQ=255)

                                    aaTCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAAt                                                                                                                <  1:4337567/149‑1 (MQ=255)

                                    aaTCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAAt                                                                                                                <  2:229214/149‑1 (MQ=255)

                                     aTCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAAtt                                                                                                               >  1:2539056/1‑149 (MQ=255)

                                          cATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGt                                                                                                          >  1:2646739/1‑149 (MQ=255)

                                               tGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATc                                                                                                     >  2:2854068/1‑149 (MQ=255)

                                                 aTAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCaa                                                                                                   >  1:1082924/1‑149 (MQ=255)

                                                 aTAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCaa                                                                                                   <  1:3476692/149‑1 (MQ=255)

                                                   aaaTATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAaga                                                                                                 <  2:4094973/149‑1 (MQ=255)

                                                    aaTATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag                                                                                                >  1:4489170/1‑149 (MQ=255)

                                                    aaTATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAagag                                                                                                <  2:4066352/149‑1 (MQ=255)

                                                        tCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTc                                                                                            <  2:3442576/149‑1 (MQ=255)

                                                        tCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTc                                                                                            <  1:179753/149‑1 (MQ=255)

                                                           ttAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGa                                                                                         >  1:3005877/1‑149 (MQ=255)

                                                             aaTATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc                                                                                       >  2:812058/1‑149 (MQ=255)

                                                                 tatTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTg                                                                                   >  2:1535285/1‑149 (MQ=255)

                                                                 tatTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTg                                                                                   >  1:4410569/1‑149 (MQ=255)

                                                                  atTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGTGTCGCCCCTGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa                                                                                  <  1:2783102/149‑1 (MQ=255)

                                                                  atTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa                                                                                  <  2:5148827/149‑1 (MQ=255)

                                                                  atTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa                                                                                  >  2:1054453/1‑149 (MQ=255)

                                                                   ttGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAg                                                                                 >  2:1695274/1‑149 (MQ=255)

                                                                    tgtgCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAgg                                                                                <  2:4613802/149‑1 (MQ=255)

                                                                     gtgCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGt                                                                               <  1:2433969/149‑1 (MQ=255)

                                                                       gcagcaATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaa                                                                             >  1:3264294/1‑149 (MQ=255)

                                                                        cagcaATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaaa                                                                            <  1:1121005/149‑1 (MQ=255)

                                                                         agcaATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaaaa                                                                           >  2:3048136/1‑149 (MQ=255)

                                                                             aTGTGTCTTCATACTATTCGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                       <  1:950763/149‑1 (MQ=255)

                                                                             aTGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGTAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                       <  1:4783350/149‑1 (MQ=255)

                                                                             aTGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                       >  1:5153413/1‑149 (MQ=255)

                                                                             aTGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc                                                                       >  2:586980/1‑149 (MQ=255)

                                                                                  tCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTa                                                                  <  1:4751715/149‑1 (MQ=255)

                                                                                    ggCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAgg                                                                >  2:1794674/1‑149 (MQ=255)

                                                                                     gCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGt                                                               <  2:2554435/149‑1 (MQ=255)

                                                                                     gCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGt                                                               >  1:5199090/1‑149 (MQ=255)

                                                                                       aTACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAt                                                             >  2:3741852/1‑149 (MQ=255)

                                                                                        tACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAtt                                                            >  1:4812486/1‑149 (MQ=255)

                                                                                        tACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAtt                                                            >  1:227929/1‑149 (MQ=255)

                                                                                         aCTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAttt                                                           >  1:1823356/1‑149 (MQ=255)

                                                                                          cTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAAGTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAtttt                                                          <  2:3688027/149‑1 (MQ=255)

                                                                                           tataCGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAttttt                                                         <  2:3240811/149‑1 (MQ=255)

                                                                                             taCGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCa                                                       >  1:2393755/1‑149 (MQ=255)

                                                                                                          tCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCaa                                          >  1:2248089/1‑149 (MQ=255)

                                                                                                              cccGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGt                                      >  1:3740350/1‑149 (MQ=255)

                                                                                                               ccGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGtt                                     <  1:5049119/149‑1 (MQ=255)

                                                                                                               ccGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGtt                                     >  1:422323/1‑149 (MQ=255)

                                                                                                                cGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGttt                                    >  2:557353/1‑149 (MQ=255)

                                                                                                                 ggAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTg                                   >  2:3894296/1‑149 (MQ=255)

                                                                                                                  gAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGa                                  <  2:1458364/149‑1 (MQ=255)

                                                                                                                   aaaaTAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAg                                 <  2:489211/149‑1 (MQ=255)

                                                                                                                   aaaaTAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAg                                 >  1:2516991/1‑149 (MQ=255)

                                                                                                                   aaaaTAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAg                                 <  2:3089619/149‑1 (MQ=255)

                                                                                                                      aTAACATTGACCATTAAGTTTAATTCTGGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTaa                              <  2:1150853/149‑1 (MQ=255)

                                                                                                                         aCATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtg                           >  1:2019695/1‑149 (MQ=255)

                                                                                                                         aCATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtg                           >  1:1555456/1‑149 (MQ=255)

                                                                                                                          cATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtgt                          <  1:3809963/149‑1 (MQ=255)

                                                                                                                           aTTGACCATTAAGTTTAATTCTTTGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTc                         <  2:1981438/149‑1 (MQ=255)

                                                                                                                             tGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCgt                       <  2:1176805/149‑1 (MQ=255)

                                                                                                                                  aTTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGtttt                  <  2:2393755/149‑1 (MQ=255)

                                                                                                                                   ttAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTc                 <  1:1470180/149‑1 (MQ=255)

                                                                                                                                     aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGt               <  2:2390874/149‑1 (MQ=255)

                                                                                                                                        tttAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGtttt            <  1:2323331/149‑1 (MQ=255)

                                                                                                                                         ttAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGttttt           <  2:2667125/149‑1 (MQ=255)

                                                                                                                                            aTTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATAAAGAGTGTCTGAGCGTTGAGGTAAAATTTACATTAGGTATTTTTCAAATATTTGGGCTATGGTTTGTGTAAGTGTCGTGTTTTCGTTTttatt        >  2:2890685/1‑149 (MQ=255)

                                                                                                                                             ttCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTttatta       <  2:2790324/149‑1 (MQ=255)

                                                                                                                                                ttGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTattaatt    <  2:3870/149‑1 (MQ=255)

                                                                                                                                                 tGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTattaatta   >  2:1260876/1‑149 (MQ=255)

TTTCAGCTGAAGAGTAATCATCTTGATGGGGTTTGTAATCAACATTCTGATAAATATCATTAATATATTGTGCAGCAATGTGTCGGCATACTATACGGTTTTGGGGTCGCCCCGGAAAATAACATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTAT  >  CP009273/4211866‑4212160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`