| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 361,806 | G→A | 100% | intergenic (‑63/+14) | lacI ← / ← mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 361,806 | 0 | G | A | 96.7% | 28573.8 / inf | 7747 | intergenic (‑63/+14) | lacI/mhpR | DNA‑binding transcriptional repressor/mhp operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (138/121); new base A (3791/3688); total (3932/3815) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 7754 total aligned reads displayed.
TGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCT > CP009273/361658‑361954 | ggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt < 1:3473920/149‑1 (MQ=255) ggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt < 2:4480658/149‑1 (MQ=255) ggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCAtt < 1:162782/149‑1 (MQ=255) gcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTc < 2:5063619/149‑1 (MQ=255) ggTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATg > 1:3142795/1‑149 (MQ=255) gTTCTCCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATgg < 2:1442730/149‑1 (MQ=255) ttCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGgt > 2:2100496/1‑149 (MQ=255) tCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg < 2:872581/149‑1 (MQ=255) tCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg < 2:4311443/149‑1 (MQ=255) tCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg < 1:1542869/149‑1 (MQ=255) caccacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtgt < 2:193026/149‑1 (MQ=255) caccacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtgt < 2:104930/149‑1 (MQ=255) caccacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtgt < 1:762295/149‑1 (MQ=255) cacGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCaa < 1:146232/149‑1 (MQ=255) cgcgGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAtt > 1:1284386‑M2/1‑147 (MQ=255) cgGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaa > 1:5070780‑M2/1‑145 (MQ=255) gAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatta < 1:4157749‑M2/149‑8 (MQ=255) gAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaatta < 2:3941219‑M2/149‑8 (MQ=255) aaaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaa < 1:5161425‑M2/149‑9 (MQ=255) aaCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaa < 2:320699‑M2/149‑10 (MQ=255) aCGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaag > 1:1782508‑M2/1‑139 (MQ=255) gTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggca > 1:2960589‑M2/1‑136 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATTGTGTCAAgtaataaaaggcatc < 1:1700519‑M2/149‑16 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatc < 2:4571157‑M2/149‑16 (MQ=255) cTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatc > 1:2142238‑M2/1‑134 (MQ=255) tGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatca > 2:1342642‑M2/1‑133 (MQ=255) aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaa > 2:1544463‑M2/1‑131 (MQ=255) aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAGGGTGTCAAttaattaaaggcatcaaa < 1:2780557‑M2/149‑19 (MQ=255) aaGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaata > 1:270324‑M2/1‑129 (MQ=255) aaGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaata > 2:3734775‑M2/1‑129 (MQ=255) agaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaa > 1:186353‑M2/1‑126 (MQ=255) gaCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaac > 2:69052‑M2/1‑125 (MQ=255) cacCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacga < 1:4622435‑M2/149‑27 (MQ=255) acCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaa < 2:114321‑M2/149‑28 (MQ=255) acCGGCATACTCTGCGACATCGTATAACGTTACTGGTATCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaa < 1:666151‑M2/149‑28 (MQ=255) cATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggct < 2:4870066‑M2/149‑33 (MQ=255) cATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggct < 2:3537045‑M2/149‑33 (MQ=255) aCTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATCCCGCGAAAGGTTTTGCCCCCTTCGATGGGGTCACtgaattaaaggcatcaaataaaacgaaaggctcag < 1:454559‑M2/149‑36 (MQ=255) aCTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcag > 1:3045984‑M2/1‑114 (MQ=255) tctGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGaa > 1:4713779/1‑149 (MQ=255) ctGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcg > 1:4438409‑M2/1‑111 (MQ=255) gCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaa < 2:2365098‑M2/149‑41 (MQ=255) gCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaa > 2:473836‑M2/1‑109 (MQ=255) cGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaa > 1:4246678‑M2/1‑108 (MQ=255) cATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagac > 1:2218489‑M2/1‑105 (MQ=255) cATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagac > 2:4221531‑M2/1‑105 (MQ=255) aTCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagact < 1:4339189‑M2/149‑46 (MQ=255) tCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactg < 1:4980764‑M2/149‑47 (MQ=255) tCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactg < 1:2507442‑M2/149‑47 (MQ=255) tCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactg > 1:1381070‑M2/1‑103 (MQ=255) tCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAGGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactg < 2:4135746‑M2/149‑47 (MQ=255) ataACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc < 2:3413479‑M2/149‑51 (MQ=255) ataACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc < 2:4392506‑M2/149‑51 (MQ=255) taACGTTACTGGTTTCACATTCAACACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGAGGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct < 2:592472‑M2/149‑52 (MQ=255) aaCTTTACGGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt > 1:2871156‑M2/1‑97 (MQ=255) aCGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt < 2:2193598‑M2/149‑54 (MQ=255) ttACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttcattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt < 1:3396335‑M2/149‑57 (MQ=255) ttACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctctgtcgaaagactgggcctttcgt < 2:2276796‑M2/149‑57 (MQ=255) tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt < 1:3947231‑M2/149‑58 (MQ=255) tACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt < 2:4652014‑M2/149‑58 (MQ=255) aCTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt > 1:846151‑M2/1‑91 (MQ=255) cTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt < 2:1242657‑M2/149‑60 (MQ=255) tGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta > 1:3660443‑M2/1‑89 (MQ=255) ggTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat > 1:3304208‑M2/1‑88 (MQ=255) tttCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct > 2:3306901‑M2/1‑86 (MQ=255) acaTTCCCCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg > 2:4022670‑M2/1‑82 (MQ=255) caTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt < 2:5177332‑M2/149‑69 (MQ=255) caTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt < 1:479759‑M2/149‑69 (MQ=255) aTTCACCACCCTTAATTGACTCTCTGCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt > 1:2315316‑M2/1‑80 (MQ=255) aTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt < 2:2018206‑M2/149‑70 (MQ=255) ttCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcttcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtgtgatctgttgttt < 2:2945361‑M2/149‑71 (MQ=255) ttCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt < 1:3867336‑M2/149‑71 (MQ=255) accaccCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc < 1:4044833‑M2/149‑74 (MQ=255) ccaccCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg > 2:1826577‑M2/1‑75 (MQ=255) cccccaagtgacGCTCTTCCGCGCGATAGCATGCCATACCGCCAAACGTTTTGCACCATTCGAGGGTGTCAAttaatgaaacgcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg > 1:3225854‑M2/6‑72 (MQ=255) tGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaatgcatcaaataaaacgaaaggcacagtcgaaagactgggcctttaggtttatctgttgtttgtctgtgaac < 1:2409850‑M2/149‑81 (MQ=255) tGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac > 1:4883408‑M2/1‑69 (MQ=255) aTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct > 2:4963158‑M2/1‑66 (MQ=255) aTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct < 2:1647660‑M2/149‑84 (MQ=255) aTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct < 2:3634019‑M2/149‑84 (MQ=255) aTTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct < 2:302855‑M2/149‑84 (MQ=255) aCTCTCTTCCGGGCGCGATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcc > 1:3570815‑M2/1‑62 (MQ=255) ctctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcct > 2:1735924‑M2/1‑61 (MQ=255) ctctctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcct < 2:2640946‑M2/149‑89 (MQ=255) ctTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagt > 1:563934‑M2/1‑57 (MQ=255) cGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtagga < 2:2764637‑M2/149‑97 (MQ=255) gggCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggac < 2:2457670‑M2/149‑98 (MQ=255) cgcTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaa > 1:1196036‑M2/1‑49 (MQ=255) cTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatc < 1:3768014‑M2/149‑103 (MQ=255) tATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatcc > 2:2865116‑M2/1‑46 (MQ=255) tATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaataaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggccgtgcgtgttagctggtgtttgtcggtgaacgctctcctgaggaggacaaatcc < 2:3119056‑M2/149‑104 (MQ=255) aTCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTc > 2:788906/1‑149 (MQ=255) aTCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccg < 2:3841103‑M2/149‑105 (MQ=255) tGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgc < 1:2603035‑M2/149‑109 (MQ=255) cATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccct > 1:1962058‑M2/1‑38 (MQ=255) aTACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgcccta > 1:2063958‑M2/1‑37 (MQ=255) aTACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAAttaattaaaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgcccta < 1:1003023‑M2/149‑113 (MQ=255) gcgAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCa > 1:935371/1‑149 (MQ=255) gcgAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCa > 2:255882/1‑149 (MQ=255) cgcCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTgcgc < 1:1110440/149‑1 (MQ=255) | TGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCT > CP009273/361658‑361954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |