BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,540,328	G→T	5.1%	I262I (ATC→ATA)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,540,328	0	G	T	5.1%	1235.6 / 33.0	315	I262I (ATC→ATA)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand): ref base G (139/160); new base T (9/7); total (148/167)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 319 total aligned reads displayed.

CCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCGGTTGATCTG  >  CP009273/4540180‑4540476

ccGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCg                                                                                                                                                      >  2:3079529/1‑149 (MQ=255)

   cACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATa                                                                                                                                                   >  2:410297/1‑149 (MQ=255)

     ccGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATaaa                                                                                                                                                 >  2:4322011/1‑149 (MQ=255)

       gatgatCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGt                                                                                                                                               >  2:1746190/1‑149 (MQ=255)

          gatCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTaa                                                                                                                                            <  1:2988305/149‑1 (MQ=255)

          gatCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTaa                                                                                                                                            >  1:1889309/1‑149 (MQ=255)

             cAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCa                                                                                                                                         >  2:302906/1‑149 (MQ=255)

              aGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCAc                                                                                                                                        >  1:4784541/1‑149 (MQ=255)

                 aTCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAATAGTACTAATGAAACCACCATCGAAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTc                                                                                                                                     <  1:1347697/149‑1 (MQ=255)

                  tCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACACACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTAAGAAACCAACCACCATCGCAATAAACATTGAAATCGGCGAGGAACCGATAAAGTTAACCACTTcc                                                                                                                                    >  1:3120824/1‑149 (MQ=255)

                   cACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCACAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTccc                                                                                                                                   >  1:1309366/1‑149 (MQ=255)

                    aCCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCca                                                                                                                                  >  2:4519334/1‑149 (MQ=255)

                             aTACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGt                                                                                                                         <  1:1703837/149‑1 (MQ=255)

                              tACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTa                                                                                                                        <  2:2991354/149‑1 (MQ=255)

                               aCTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTAt                                                                                                                       <  2:5071933/149‑1 (MQ=255)

                                 tctTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCt                                                                                                                     <  1:2890318/149‑1 (MQ=255)

                                  ctTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCtt                                                                                                                    >  1:4592418/1‑149 (MQ=255)

                                    tCACCGCGCTTTCACAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCtttt                                                                                                                  <  2:1068574/149‑1 (MQ=255)

                                     cACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTa                                                                                                                 <  2:5203819/149‑1 (MQ=255)

                                     cACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTa                                                                                                                 <  1:685655/149‑1 (MQ=255)

                                     cACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTa                                                                                                                 <  2:652904/149‑1 (MQ=255)

                                      aCCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTAc                                                                                                                <  1:4302477/149‑1 (MQ=255)

                                      aCCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTAc                                                                                                                <  2:4021638/149‑1 (MQ=255)

                                         gcgcTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCTATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTAccag                                                                                                             >  1:83892/1‑149 (MQ=255)

                                           gcTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTAccagcc                                                                                                           >  2:601870/1‑149 (MQ=255)

                                               tCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGat                                                                                                       >  1:551671/1‑149 (MQ=255)

                                               tCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGat                                                                                                       >  2:312021/1‑149 (MQ=255)

                                                cAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGata                                                                                                      >  2:71418/1‑149 (MQ=255)

                                                cAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGata                                                                                                      <  2:1182988/149‑1 (MQ=255)

                                                cAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGata                                                                                                      >  1:2427977/1‑149 (MQ=255)

                                                 aaaaGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGatat                                                                                                     <  1:3053653/149‑1 (MQ=255)

                                                 aaaaGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGatat                                                                                                     <  2:4182512/149‑1 (MQ=255)

                                                  aaaGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATAtt                                                                                                    <  2:3020571/149‑1 (MQ=255)

                                                    aGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTgg                                                                                                  >  2:862500/1‑149 (MQ=255)

                                                    aGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTgg                                                                                                  <  1:4818269/149‑1 (MQ=255)

                                                    aGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTgg                                                                                                  <  1:2223945/149‑1 (MQ=255)

                                                    aGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTgg                                                                                                  <  2:3306413/149‑1 (MQ=255)

                                                     gCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGc                                                                                                 >  2:1087079/1‑149 (MQ=255)

                                                     gCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGc                                                                                                 <  2:1923543/149‑1 (MQ=255)

                                                      cGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCg                                                                                                <  1:2761006/149‑1 (MQ=255)

                                                        ttCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAt                                                                                              <  1:4489886/149‑1 (MQ=255)

                                                        ttCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAt                                                                                              >  1:4128079/1‑149 (MQ=255)

                                                          catcaCCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAtgg                                                                                            >  2:1219518/1‑149 (MQ=255)

                                                          catcaCCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAtgg                                                                                            <  1:1285406/149‑1 (MQ=255)

                                                            tcaCCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAtggtg                                                                                          <  1:982871/149‑1 (MQ=255)

                                                             caCCCACTGCATGTCATGTCCACGTGCGGTGCCAAAGAGTAAGAATGCAACCACCATCGCAATAAACATTACAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAtggtgg                                                                                         <  2:4857993/149‑1 (MQ=255)

                                                             caCCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAtggtgg                                                                                         <  1:5085505/149‑1 (MQ=255)

                                                             caCCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTACAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAtggtgg                                                                                         <  2:2253846/149‑1 (MQ=255)

                                                               cccACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTa                                                                                       >  2:4260106/1‑149 (MQ=255)

                                                               cccACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTa                                                                                       >  2:2653318/1‑149 (MQ=255)

                                                                 cACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTaga                                                                                     >  1:2502349/1‑149 (MQ=255)

                                                                        tGTCATGACCACGTGCGGTGCC‑AAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGAGGGGGGTAGAGATCAtca                                                                             >  1:3077153/1‑147 (MQ=255)

                                                                         gTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATaa                                                                             >  2:4621421/1‑149 (MQ=255)

                                                                               aCCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAAAGATCg                                                                       >  2:2344374/1‑149 (MQ=255)

                                                                                ccACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGc                                                                      <  2:4686288/149‑1 (MQ=255)

                                                                                ccACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGc                                                                      <  1:3821515/149‑1 (MQ=255)

                                                                                 cACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGcc                                                                     <  2:4772222/149‑1 (MQ=255)

                                                                                  aCGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCg                                                                    >  1:1064529/1‑149 (MQ=255)

                                                                                     tGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGa                                                                 <  1:4564599/149‑1 (MQ=255)

                                                                                        ggTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCa                                                              >  1:181392/1‑149 (MQ=255)

                                                                                                cgagTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCAcc                                                      <  2:4941723/148‑1 (MQ=255)

                                                                                                 gagTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCa                                                     >  2:673966/1‑149 (MQ=255)

                                                                                                     aCGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAAt                                                 <  2:5157093/149‑1 (MQ=255)

                                                                                                         aTGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGaa                                             >  1:4193937/1‑149 (MQ=255)

                                                                                                         aTGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGaa                                             >  2:1693332/1‑149 (MQ=255)

                                                                                                           gCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAAc                                           <  2:1343879/149‑1 (MQ=255)

                                                                                                           gCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAAc                                           <  2:4784541/149‑1 (MQ=255)

                                                                                                           gCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAAc                                           <  2:2477274/149‑1 (MQ=255)

                                                                                                              accaccATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGcc                                        >  1:2072123/1‑149 (MQ=255)

                                                                                                                  ccaTCGCAATAAACATTGCAATCGGCGAGGAACCTATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGaaa                                    <  1:2853371/149‑1 (MQ=255)

                                                                                                                  ccaTCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGaaa                                    <  2:3367376/149‑1 (MQ=255)

                                                                                                                    aTCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAaga                                  >  2:1028056/1‑149 (MQ=255)

                                                                                                                    aTCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAaga                                  >  1:539710/1‑149 (MQ=255)

                                                                                                                      cGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAgg                                <  1:4912449/149‑1 (MQ=255)

                                                                                                                       gCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAggg                               >  1:214465/1‑149 (MQ=255)

                                                                                                                       gCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAggg                               >  1:4410800/1‑149 (MQ=255)

                                                                                                                       gCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAggg                               <  2:1334416/149‑1 (MQ=255)

                                                                                                                            aaaCATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGat                          <  1:5023900/149‑1 (MQ=255)

                                                                                                                              aCATTGCAATCGGCGAGGAACCTATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGatta                        <  2:2072349/149‑1 (MQ=255)

                                                                                                                              aCATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGatta                        >  1:2981673/1‑149 (MQ=255)

                                                                                                                              aCATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGatta                        >  1:1916466/1‑149 (MQ=255)

                                                                                                                                aTTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGattatt                      <  1:1684051/149‑1 (MQ=255)

                                                                                                                                  tGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCa                    <  1:3697456/149‑1 (MQ=255)

                                                                                                                                  tGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCa                    >  1:121742/1‑149 (MQ=255)

                                                                                                                                  tGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCa                    >  1:3592413/1‑149 (MQ=255)

                                                                                                                                   gCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCat                   >  1:4281348/1‑149 (MQ=255)

                                                                                                                                     aaTCGGCGAGGAACCGATAAAGTTAACCACTTTCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCatat                 >  2:2853399/1‑149 (MQ=255)

                                                                                                                                     aaTCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCatat                 >  1:35496/1‑149 (MQ=255)

                                                                                                                                     aaTCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCatat                 >  1:282511/1‑149 (MQ=255)

                                                                                                                                      aTCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATc                >  1:4001770/1‑149 (MQ=255)

                                                                                                                                      aTCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATc                >  1:1548899/1‑149 (MQ=255)

                                                                                                                                       tCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCt               <  1:1788243/149‑1 (MQ=255)

                                                                                                                                        cGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTa              <  2:3671937/149‑1 (MQ=255)

                                                                                                                                         ggCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTAc             <  2:3482014/149‑1 (MQ=255)

                                                                                                                                           cGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCg           <  1:3965804/149‑1 (MQ=255)

                                                                                                                                             aGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCGGt         >  2:645674/1‑149 (MQ=255)

                                                                                                                                             aGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCGGt         >  2:2295825/1‑149 (MQ=255)

                                                                                                                                                aaCCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCGGTTGa      <  2:4825477/149‑1 (MQ=255)

                                                                                                                                                aaCCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCGGTTGa      <  1:1036883/149‑1 (MQ=255)

                                                                                                                                                    gATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCGGTTGATCTg  >  2:2158411/1‑149 (MQ=255)

CCGCACCGATGATCAGAATCACCATTGCAATACTCTTCACCGCGCTTTCAAAAGCGTTCATCACCCACTGCATGTCATGACCACGTGCGGTGCCAAAGAGTACGAATGCAACCACCATCGCAATAAACATTGCAATCGGCGAGGAACCGATAAAGTTAACCACTTCCCAGGCAGGGGTATCTTTTACCAGCCAGATATTGGCGATGGTGGTAGAGATCATAATGATCGCCGGGATCAGCGGCACCAGAATCGAAACGCCGAAAGAGGGCAGATTATTCATATCTACCGGTTGATCTG  >  CP009273/4540180‑4540476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`