BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	419,663	G→T	7.5%	E564* (GAA→TAA)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	419,663	0	G	T	7.5%	1179.7 / 51.9	306	E564* (GAA→TAA)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base G (132/151); new base T (13/10); total (145/161)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

CCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAC  >  CP009273/419515‑419811

ccAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCtgtg                                                                                                                                                      >  1:4803145/1‑149 (MQ=255)

  aGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGaa                                                                                                                                                    <  2:229030/149‑1 (MQ=255)

  aGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGaa                                                                                                                                                    >  2:4248497/1‑149 (MQ=255)

   gCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAg                                                                                                                                                   <  1:1287412/149‑1 (MQ=255)

   gCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAg                                                                                                                                                   <  2:3190408/149‑1 (MQ=255)

    cGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgt                                                                                                                                                  <  2:89605/149‑1 (MQ=255)

    cGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgt                                                                                                                                                  <  2:4147370/149‑1 (MQ=255)

      aaTGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtgg                                                                                                                                                >  2:805695/1‑149 (MQ=255)

        tGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtggtg                                                                                                                                              <  2:119104/149‑1 (MQ=255)

           ttGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTa                                                                                                                                           <  1:2067190/149‑1 (MQ=255)

                cTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCcgc                                                                                                                                      >  2:4499749/1‑149 (MQ=255)

                 tGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCcgcg                                                                                                                                     >  2:3423594/1‑149 (MQ=255)

                    gTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCa                                                                                                                                  >  1:44029/1‑149 (MQ=255)

                    gTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCa                                                                                                                                  >  1:44071/1‑149 (MQ=255)

                       aGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCg                                                                                                                               >  1:1147573/1‑149 (MQ=255)

                       aGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCg                                                                                                                               >  1:3764698/1‑149 (MQ=255)

                         aaaaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGtt                                                                                                                             <  1:4914423/149‑1 (MQ=255)

                                 ggCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATg                                                                                                                     >  1:2447567/1‑149 (MQ=255)

                                     cTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGt                                                                                                                 <  1:1442321/149‑1 (MQ=255)

                                     cTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGt                                                                                                                 <  1:1014237/149‑1 (MQ=255)

                                     cTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGt                                                                                                                 <  2:2194449/149‑1 (MQ=255)

                                         gTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaa                                                                                                             <  2:2363792/149‑1 (MQ=255)

                                          tCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGAGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaat                                                                                                            <  2:4588965/149‑1 (MQ=255)

                                            aTGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatgg                                                                                                          <  2:1245658/149‑1 (MQ=255)

                                            aTGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatgg                                                                                                          >  1:4887422/1‑149 (MQ=255)

                                              ggcggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggca                                                                                                        <  2:4812580/149‑1 (MQ=255)

                                                cggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCGACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaat                                                                                                      <  2:5106059/149‑1 (MQ=255)

                                                    tGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCaa                                                                                                  >  1:4913340/1‑149 (MQ=255)

                                                     gTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCaaa                                                                                                 <  1:3498085/149‑1 (MQ=255)

                                                     gTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCaaa                                                                                                 >  2:1069236/1‑149 (MQ=255)

                                                      tCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaag                                                                                                >  2:2962383/1‑149 (MQ=255)

                                                      tCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaag                                                                                                >  2:1980746/1‑149 (MQ=255)

                                                       cAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaaga                                                                                               <  1:547562/149‑1 (MQ=255)

                                                        aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCGATGCCGTGCAATGGCAATGCAaagaa                                                                                              <  2:1486787/149‑1 (MQ=255)

                                                        aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaa                                                                                              >  2:244048/1‑149 (MQ=255)

                                                        aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaa                                                                                              >  2:4903191/1‑149 (MQ=255)

                                                          gTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAgg                                                                                            <  1:2676102/149‑1 (MQ=255)

                                                          gTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCGACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAgg                                                                                            <  2:729464/149‑1 (MQ=255)

                                                           tGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAggg                                                                                           <  1:5131675/149‑1 (MQ=255)

                                                            gCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGc                                                                                          <  1:3855808/149‑1 (MQ=255)

                                                             cTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCa                                                                                         >  1:1903046/1‑149 (MQ=255)

                                                              tGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCAt                                                                                        <  2:195062/149‑1 (MQ=255)

                                                               gTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATg                                                                                       >  1:4278120/1‑149 (MQ=255)

                                                                tATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATgg                                                                                      >  1:4551442/1‑149 (MQ=255)

                                                                 aTGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATggg                                                                                     >  1:472911/1‑149 (MQ=255)

                                                                      gAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAact                                                                                >  1:2653772/1‑149 (MQ=255)

                                                                      gAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAact                                                                                >  2:3823418/1‑149 (MQ=255)

                                                                        aGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactga                                                                              >  1:3141266/1‑149 (MQ=255)

                                                                         gATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgac                                                                             >  1:2968031/1‑149 (MQ=255)

                                                                         gATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgac                                                                             <  2:2099491/149‑1 (MQ=255)

                                                                          aTAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgact                                                                            <  1:3872062/149‑1 (MQ=255)

                                                                              cGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACg                                                                        <  1:62271/149‑1 (MQ=255)

                                                                                tggtggTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACggg                                                                      >  1:3793727/1‑149 (MQ=255)

                                                                                 ggtggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGa                                                                     >  1:3243934/1‑149 (MQ=255)

                                                                                 ggtggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGa                                                                     <  2:1297621/149‑1 (MQ=255)

                                                                                  gtgggATGTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAAAGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAt                                                                    <  1:999327/149‑1 (MQ=255)

                                                                                   tggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAtt                                                                   >  1:756481/1‑149 (MQ=255)

                                                                                    ggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTc                                                                  <  1:883907/149‑1 (MQ=255)

                                                                                     gtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCt                                                                 >  1:225805/1‑149 (MQ=255)

                                                                                        tttGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGc                                                              <  1:256663/149‑1 (MQ=255)

                                                                                        tttGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGc                                                              >  2:364511/1‑149 (MQ=255)

                                                                                        tttGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGc                                                              <  2:2483432/149‑1 (MQ=255)

                                                                                          tGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCtt                                                            >  2:1346910/1‑149 (MQ=255)

                                                                                             ccGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgc                                                         <  2:1650776/149‑1 (MQ=255)

                                                                                             ccGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgc                                                         <  2:3961184/149‑1 (MQ=255)

                                                                                                  tGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgcctgcc                                                    <  1:5023922/149‑1 (MQ=255)

                                                                                                       aaTCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTc                                               <  2:44071/149‑1 (MQ=255)

                                                                                                       aaTCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTc                                               >  2:2891990/1‑149 (MQ=255)

                                                                                                        aTCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCg                                              >  2:4326001/1‑149 (MQ=255)

                                                                                                        aTCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCg                                              >  1:716808/1‑149 (MQ=255)

                                                                                                           aGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCt                                           >  2:823925/1‑149 (MQ=255)

                                                                                                           aGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCt                                           <  1:1909306/149‑1 (MQ=255)

                                                                                                            gCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                          >  1:2771548/1‑149 (MQ=255)

                                                                                                             cAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTAc                                         <  2:3579945/149‑1 (MQ=255)

                                                                                                              aaCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACg                                        >  2:47153/1‑149 (MQ=255)

                                                                                                               aCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                       >  2:1861035/1‑149 (MQ=255)

                                                                                                                  tgtACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTAt                                    >  1:3357387/1‑149 (MQ=255)

                                                                                                                  tgtACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTAt                                    <  2:2645035/149‑1 (MQ=255)

                                                                                                                    tACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATgg                                  >  2:2355068/1‑149 (MQ=255)

                                                                                                                     aCTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                 <  1:2907178/149‑1 (MQ=255)

                                                                                                                        ggtggCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGa                              <  2:1744114/149‑1 (MQ=255)

                                                                                                                             cAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTaa                         >  2:3115272/1‑149 (MQ=255)

                                                                                                                             cAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTaa                         <  2:1098990/149‑1 (MQ=255)

                                                                                                                                 cAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGtt                     <  1:1608760/149‑1 (MQ=255)

                                                                                                                                   aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGtttt                   <  1:4149842/149‑1 (MQ=255)

                                                                                                                                    ccGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                  <  1:3848950/149‑1 (MQ=255)

                                                                                                                                    ccGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                  <  1:2357803/149‑1 (MQ=255)

                                                                                                                                    ccGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                  <  1:3961346/149‑1 (MQ=255)

                                                                                                                                    ccGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                  <  1:3696985/149‑1 (MQ=255)

                                                                                                                                      gTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtata                >  1:1671387/1‑149 (MQ=255)

                                                                                                                                       tGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATaa               <  2:4191573/149‑1 (MQ=255)

                                                                                                                                          cGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGc            <  2:3311571/149‑1 (MQ=255)

                                                                                                                                           gAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGcc           <  1:805695/149‑1 (MQ=255)

                                                                                                                                             ggCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCtt         <  2:3685001/149‑1 (MQ=255)

                                                                                                                                               ccTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGc       <  2:553062/149‑1 (MQ=255)

                                                                                                                                               ccTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGc       <  2:69645/149‑1 (MQ=255)

                                                                                                                                                cTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCg      <  1:1363182/149‑1 (MQ=255)

                                                                                                                                                  gtgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTg    <  1:1223748/149‑1 (MQ=255)

                                                                                                                                                    gAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAc  <  1:1069060/149‑1 (MQ=255)

                                                                                                                                                    gAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAc  <  1:4499749/149‑1 (MQ=255)

CCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAC  >  CP009273/419515‑419811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`