Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,915,998 C→A 100% A116S (GCT→TCT)  yqcA ← short‑chain flavodoxin, FMN‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,915,9980CA98.7% 1035.2 / 2.4 316A116S (GCT→TCT) yqcAshort‑chain flavodoxin, FMN‑binding
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (153/159);  total (154/162)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 317 total aligned reads displayed.

ATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAATGC  >  CP009273/2915852‑2916144
                                                                                                                                                  |                                                                                                                                                  
attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGct                                                                                                                                                 >  2:1414958/1‑150 (MQ=255)
  tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACACCAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGctct                                                                                                                                               <  2:414703/150‑1 (MQ=255)
    aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGt                                                                                                                                             >  1:1733105/1‑150 (MQ=255)
        ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGGTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCt                                                                                                                                         <  1:1412788/150‑1 (MQ=255)
         cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTg                                                                                                                                        <  1:892084/150‑1 (MQ=255)
           cTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGca                                                                                                                                      >  1:356441/1‑150 (MQ=255)
             aaaaCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaac                                                                                                                                    >  2:2458271/1‑150 (MQ=255)
             aaaaCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaac                                                                                                                                    >  1:2905046/1‑150 (MQ=255)
                aCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAg                                                                                                                                 <  1:1055802/150‑1 (MQ=255)
                aCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAg                                                                                                                                 <  1:737318/150‑1 (MQ=255)
                 cGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAgg                                                                                                                                <  2:1634542/150‑1 (MQ=255)
                  gACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGc                                                                                                                               <  2:1306337/150‑1 (MQ=255)
                   aCATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCa                                                                                                                              >  1:2114940/1‑150 (MQ=255)
                   aCATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCa                                                                                                                              >  1:2195468/1‑150 (MQ=255)
                   aCATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCa                                                                                                                              >  1:4960179/1‑150 (MQ=255)
                    cATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCAt                                                                                                                             >  2:1922650/1‑150 (MQ=255)
                    cATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCAt                                                                                                                             >  2:2669185/1‑150 (MQ=255)
                    cATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCAt                                                                                                                             >  1:4281373/1‑150 (MQ=255)
                     aTTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATc                                                                                                                            >  1:1864442/1‑150 (MQ=255)
                     aTTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATc                                                                                                                            >  1:1308393/1‑150 (MQ=255)
                       ttACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGa                                                                                                                          <  1:2958887/150‑1 (MQ=255)
                       ttACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGa                                                                                                                          <  1:4529543/150‑1 (MQ=255)
                       ttACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCAATTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGa                                                                                                                          >  2:2772918/1‑150 (MQ=255)
                           cgctcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTg                                                                                                                      <  2:4825842/150‑1 (MQ=255)
                             ctcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGtt                                                                                                                    <  1:4150616/150‑1 (MQ=255)
                              tcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGttt                                                                                                                   <  1:4033612/150‑1 (MQ=255)
                               cgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTg                                                                                                                  <  2:3489330/150‑1 (MQ=255)
                                  tGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTgccg                                                                                                               >  1:4904199/1‑150 (MQ=255)
                                      cATATCAGGACAAGAGCGTGCCCCACTGTTAGACCCACGGATTTGTTTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCATTCATCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCAt                                                                                                           <  2:4400616/150‑1 (MQ=255)
                                       atatCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCAtt                                                                                                          <  1:2826287/150‑1 (MQ=255)
                                        tatCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTg                                                                                                         <  2:475509/150‑1 (MQ=255)
                                        tatCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTg                                                                                                         >  1:59339/1‑150 (MQ=255)
                                         atCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGAATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGACCTGTTTGCCGCCATTGc                                                                                                        >  2:4235048/1‑150 (MQ=255)
                                           cAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAg                                                                                                      >  2:4677413/1‑150 (MQ=255)
                                            aGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGa                                                                                                     >  2:3865315/1‑150 (MQ=255)
                                             ggACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaa                                                                                                    >  1:1561457/1‑150 (MQ=255)
                                               acaacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAAt                                                                                                  <  2:2339485/150‑1 (MQ=255)
                                                caacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAAtt                                                                                                 <  2:128136/150‑1 (MQ=255)
                                                caacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAAtt                                                                                                 >  1:3647422/1‑150 (MQ=255)
                                                caacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAAtt                                                                                                 <  2:513076/150‑1 (MQ=255)
                                                     gCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACAt                                                                                            <  1:3254975/150‑1 (MQ=255)
                                                      cGTGCCCCACTGTTCGACCCACGGATTTGATTCCGGTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGATCTGGTCCTGCAACAAGGAATAGAACTGTTTGCCGCCATTGCAGAAATTCACATa                                                                                           >  1:1443284/1‑150 (MQ=255)
                                                        tGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAac                                                                                         <  1:508470/150‑1 (MQ=255)
                                                         gCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAact                                                                                        <  2:749946/150‑1 (MQ=255)
                                                          ccccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAacta                                                                                       >  1:2419317/1‑150 (MQ=255)
                                                           cccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAactac                                                                                      >  2:2516555/1‑150 (MQ=255)
                                                            ccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCCTTGCAGAAATTCACATAactact                                                                                     >  2:2934810/1‑150 (MQ=255)
                                                            ccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAactact                                                                                     <  2:3987638/150‑1 (MQ=255)
                                                               cTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTc                                                                                  <  1:4385003/150‑1 (MQ=255)
                                                                    cGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGa                                                                             >  2:3238436/1‑150 (MQ=255)
                                                                     gACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAg                                                                            <  1:3958719/150‑1 (MQ=255)
                                                                      aCCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAgc                                                                           <  2:3351501/150‑1 (MQ=255)
                                                                       cccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAgcg                                                                          <  1:644667/150‑1 (MQ=255)
                                                                       cccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAgcg                                                                          <  1:3089477/150‑1 (MQ=255)
                                                                       cccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCAGCCATTGCAGAAATTCACATAACTACTGTCGCCGAgcg                                                                          >  1:4858655/1‑150 (MQ=255)
                                                                        ccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAgcgc                                                                         <  2:4267345/150‑1 (MQ=255)
                                                                          aCGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCaa                                                                       <  1:2018653/150‑1 (MQ=255)
                                                                           cGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAAt                                                                      >  1:1353233/1‑150 (MQ=255)
                                                                            ggATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATc                                                                     >  2:2756613/1‑150 (MQ=255)
                                                                              aTTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCAc                                                                   >  1:2590220/1‑150 (MQ=255)
                                                                              aTTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCAc                                                                   >  1:3167637/1‑150 (MQ=255)
                                                                              aTTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCAc                                                                   <  1:3183792/150‑1 (MQ=255)
                                                                                ttGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGc                                                                 <  1:3459366/150‑1 (MQ=255)
                                                                                 tGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGcc                                                                <  2:899992/150‑1 (MQ=255)
                                                                                  gATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCa                                                               <  1:4126174/150‑1 (MQ=255)
                                                                                   aTTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCAt                                                              <  2:304202/150‑1 (MQ=255)
                                                                                      ccGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAAc                                                           <  1:3838077/150‑1 (MQ=255)
                                                                                         tttCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCa                                                        <  1:5136346/150‑1 (MQ=255)
                                                                                            ccggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGAt                                                     <  2:1699398/150‑1 (MQ=255)
                                                                                             cggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGAtt                                                    <  1:1604831/150‑1 (MQ=255)
                                                                                               gttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCg                                                  <  2:3586252/150‑1 (MQ=255)
                                                                                               gttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCg                                                  >  1:3368143/1‑150 (MQ=255)
                                                                                                    gggTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTgg                                             >  1:2368115/1‑150 (MQ=255)
                                                                                                        tctcACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGc                                         <  1:332332/150‑1 (MQ=255)
                                                                                                           cACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCa                                      <  1:3682823/150‑1 (MQ=255)
                                                                                                           cACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCa                                      >  1:4482375/1‑150 (MQ=255)
                                                                                                               ggCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACt                                  <  1:340139/150‑1 (MQ=255)
                                                                                                               ggCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACt                                  >  2:614526/1‑150 (MQ=255)
                                                                                                                  gTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATc                               <  1:1954357/150‑1 (MQ=255)
                                                                                                                  gTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATc                               <  1:1621479/150‑1 (MQ=255)
                                                                                                                   tcaatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCt                              <  2:4960387/150‑1 (MQ=255)
                                                                                                                     aatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCttt                            <  2:1078358/150‑1 (MQ=255)
                                                                                                                     aatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCttt                            <  2:841893/150‑1 (MQ=255)
                                                                                                                      atcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTg                           >  2:1808293/1‑150 (MQ=255)
                                                                                                                       tcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGa                          <  2:1154157/150‑1 (MQ=255)
                                                                                                                        caaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGAt                         >  2:2192117/1‑150 (MQ=255)
                                                                                                                        caaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGAt                         >  2:1220056/1‑150 (MQ=255)
                                                                                                                        caaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGAt                         <  2:2114862/150‑1 (MQ=255)
                                                                                                                             gCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCt                    <  2:2704520/150‑1 (MQ=255)
                                                                                                                                 ttCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGaaa                <  1:118208/150‑1 (MQ=255)
                                                                                                                                   cGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAaga              <  2:4796224/150‑1 (MQ=255)
                                                                                                                                     ccAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGt            <  1:862192/150‑1 (MQ=255)
                                                                                                                                      cAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTg           <  2:5329737/150‑1 (MQ=255)
                                                                                                                                       aaCCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTgg          >  2:175108/1‑150 (MQ=255)
                                                                                                                                        aCCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGc         >  2:2615531/1‑150 (MQ=255)
                                                                                                                                         cccGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGca        >  1:5289816/1‑150 (MQ=255)
                                                                                                                                           cGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGcaca      >  2:226159/1‑150 (MQ=255)
                                                                                                                                             cTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAAt    <  2:3369151/150‑1 (MQ=255)
                                                                                                                                             cTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAAt    <  1:1256848/150‑1 (MQ=255)
                                                                                                                                              tGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAATg   <  2:4545607/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAATGC  >  CP009273/2915852‑2916144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑