Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,921,101 C→A 100% intergenic (+70/‑487) ygdH → / → sdaC UPF0717 family protein/putative serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,921,1010CA98.4% 1002.6 / 5.1 314intergenic (+70/‑487)ygdH/sdaCUPF0717 family protein/putative serine transporter
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (132/177);  total (133/181)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 337 total aligned reads displayed.

CTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGAT  >  CP009273/2920954‑2921247
                                                                                                                                                   |                                                                                                                                                  
cTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACtcatttaat                                                                                                                                                  >  1:1777065/1‑150 (MQ=255)
 tACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACtcatttaatt                                                                                                                                                 >  1:204820/1‑150 (MQ=255)
   cAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTg                                                                                                                                               >  1:4860352/1‑150 (MQ=255)
       gTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTtat                                                                                                                                           <  2:4441608/150‑1 (MQ=255)
          ttgttgCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATg                                                                                                                                        <  1:3269159/150‑1 (MQ=255)
           tgttgCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCCACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGa                                                                                                                                       <  2:1491143/150‑1 (MQ=255)
             ttgCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAAt                                                                                                                                     <  2:3198949/150‑1 (MQ=255)
                 ccAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATtttt                                                                                                                                 >  1:783291/1‑150 (MQ=255)
                  cAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTc                                                                                                                                <  2:559334/150‑1 (MQ=255)
                     cATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTa                                                                                                                             <  2:1389814/150‑1 (MQ=255)
                      aTCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTac                                                                                                                            >  2:267457/1‑150 (MQ=255)
                      aTCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTac                                                                                                                            <  2:1597840/150‑1 (MQ=255)
                         gTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTaccac                                                                                                                         >  1:111978/1‑150 (MQ=255)
                            tGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCt                                                                                                                      >  1:3666198/1‑150 (MQ=255)
                            tGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCt                                                                                                                      <  1:1516602/150‑1 (MQ=255)
                            tGAAGTTGCCAGGCTCAGCCTACATCCCTAGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCt                                                                                                                      >  1:3000978/1‑150 (MQ=255)
                             gAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTc                                                                                                                     <  1:675692/150‑1 (MQ=255)
                               aGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCAc                                                                                                                   <  2:664558/150‑1 (MQ=255)
                                     cAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACa                                                                                                             <  2:3462578/150‑1 (MQ=255)
                                          tCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAatat                                                                                                        <  1:2808230/150‑1 (MQ=255)
                                            aGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatca                                                                                                      >  1:5311138/1‑150 (MQ=255)
                                             gCCTACATCCCGTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcat                                                                                                     <  2:3145826/150‑1 (MQ=255)
                                              ccTACATCCCTTGCTAAGAAATCTGCACGTAATCTCCGCTCACCCGGTGACGTGTGATGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcatc                                                                                                    >  2:1656280/1‑150 (MQ=255)
                                               cTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATAtcatca                                                                                                   >  2:2449516/1‑150 (MQ=255)
                                                  cATCCCTTGCTTCGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAg                                                                                                <  2:5258463/150‑1 (MQ=255)
                                                   aTCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGt                                                                                               >  2:1052045/1‑150 (MQ=255)
                                                   aTCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGt                                                                                               >  2:3936995/1‑150 (MQ=255)
                                                   aTCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCACCCTGTGACGTTTGTTGCAGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATAATCACAGt                                                                                               <  2:3989162/150‑1 (MQ=255)
                                                    tCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGtt                                                                                              >  1:2086033/1‑150 (MQ=255)
                                                       cTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAat                                                                                           <  2:4997919/150‑1 (MQ=255)
                                                         tGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAatat                                                                                         <  1:267457/150‑1 (MQ=255)
                                                          gCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATg                                                                                        <  1:5029667/150‑1 (MQ=255)
                                                           cTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTTAAGTTTGTTGCCGGGTTTTCTAGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTAAATGAATGTTTCTTACCACCCTCACGCGACAAATATCAGCACAGTTAATATGt                                                                                       >  1:2170049/1‑150 (MQ=255)
                                                             aCGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTcg                                                                                     <  2:4239249/150‑2 (MQ=255)
                                                             aCGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCa                                                                                     >  2:3628438/1‑150 (MQ=255)
                                                             aCGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATGTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCa                                                                                     >  1:584412/1‑150 (MQ=255)
                                                               gAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATa                                                                                   <  2:954251/150‑1 (MQ=255)
                                                                aaaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATAc                                                                                  >  2:5223149/1‑150 (MQ=255)
                                                                  aTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACaa                                                                                <  2:3355453/150‑1 (MQ=255)
                                                                    cTGCTCGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAAtt                                                                              <  1:3511210/150‑1 (MQ=255)
                                                                     tGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAAttt                                                                             >  1:2497403/1‑150 (MQ=255)
                                                                       cACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTAt                                                                           <  1:2238021/150‑1 (MQ=255)
                                                                         cGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGt                                                                         <  2:4613076/150‑1 (MQ=255)
                                                                          gTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGtt                                                                        >  1:2933685/1‑150 (MQ=255)
                                                                          gTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGtt                                                                        >  1:1732495/1‑150 (MQ=255)
                                                                           tAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTg                                                                       <  2:4736670/150‑1 (MQ=255)
                                                                            caTCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGc                                                                      <  2:1311130/149‑1 (MQ=255)
                                                                             aTCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATAATCACAGTTAATATGTCATACAATTTATGTTGCa                                                                     >  2:3311886/1‑150 (MQ=255)
                                                                               ctcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAAc                                                                   <  2:4781366/150‑1 (MQ=255)
                                                                                tcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACg                                                                  <  1:5221308/150‑1 (MQ=255)
                                                                                tcCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACg                                                                  <  2:1114285/150‑1 (MQ=255)
                                                                                   gCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCaa                                                               >  1:4336485/1‑150 (MQ=255)
                                                                                     tCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAAc                                                             <  2:5086676/150‑1 (MQ=255)
                                                                                     tCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAAc                                                             <  1:1477512/150‑1 (MQ=255)
                                                                                     tCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAAc                                                             <  1:4750050/150‑1 (MQ=255)
                                                                                     tCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAAc                                                             <  2:3088432/150‑1 (MQ=255)
                                                                                     tCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAAc                                                             <  2:1865652/150‑1 (MQ=255)
                                                                                      cccccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACg                                                            >  2:74128/1‑150 (MQ=255)
                                                                                        cccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGtt                                                          <  1:3093975/150‑1 (MQ=255)
                                                                                         ccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGttt                                                         <  1:1037372/150‑1 (MQ=255)
                                                                                         ccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGttt                                                         <  1:3231592/150‑1 (MQ=255)
                                                                                         ccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGttt                                                         >  1:3553442/1‑150 (MQ=255)
                                                                                         ccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGttt                                                         <  2:1210460/150‑1 (MQ=255)
                                                                                         ccGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGttt                                                         >  1:1393455/1‑150 (MQ=255)
                                                                                            gTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTccc                                                      >  1:3955648/1‑150 (MQ=255)
                                                                                             tGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGAAACGCAAACGTTTCCCt                                                     >  2:1188935/1‑150 (MQ=255)
                                                                                                 gTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTAttt                                                 <  1:4009696/150‑1 (MQ=255)
                                                                                                 gTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTAttt                                                 >  2:3783004/1‑150 (MQ=255)
                                                                                                  tttGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTAtttt                                                <  1:1915308/150‑1 (MQ=255)
                                                                                                   ttgttgCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTc                                               <  2:1818675/150‑1 (MQ=255)
                                                                                                    tgttgCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCa                                              <  1:4356871/150‑1 (MQ=255)
                                                                                                       tgCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATaa                                           <  2:733733/150‑1 (MQ=255)
                                                                                                        gCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATaaa                                          <  2:3738109/150‑1 (MQ=255)
                                                                                                        gCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTAATaaa                                          >  1:5068794/1‑150 (MQ=255)
                                                                                                         ccGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAAc                                         <  1:3221627/150‑1 (MQ=255)
                                                                                                          cGGGTTTTCTAGTTTTTGGTCACTTACTCATCAAATCATTTAATTTGTTATATTAATGTTTCTTACCACACTCACGCGACAAATATAATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAAcc                                        <  1:450101/150‑1 (MQ=255)
                                                                                                            ggTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGt                                      >  1:1293089/1‑150 (MQ=255)
                                                                                                             gTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTa                                     >  1:2024465/1‑150 (MQ=255)
                                                                                                               tttCTCGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAtt                                   <  1:3328143/150‑1 (MQ=255)
                                                                                                               tttCTAGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTAtt                                   <  2:1995703/150‑1 (MQ=255)
                                                                                                                ttatagTTTTTGGTCACTTACTCTTAAAATCATTTAATTTGTTTTATGAATGTTTATTACCACCCTCACGCGACATATTTAATATATTTTAATATGTCATACAATTTATGTTGAAACGCAAACGTTTCCCTATTTTCATAAACCGTAttt                                  <  1:911303/145‑1 (MQ=255)
                                                                                                                    cGTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATc                              <  2:5135967/150‑1 (MQ=255)
                                                                                                                     gTTTTTGGTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCg                             >  2:1014190/1‑150 (MQ=255)
                                                                                                                          tGGTCACTTACTCATCAACTCATTTAATTTGTTATATTAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATg                        >  2:2326795/1‑150 (MQ=255)
                                                                                                                           ggTCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGa                       >  1:711709/1‑150 (MQ=255)
                                                                                                                             tCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGaaa                     >  1:4628658/1‑150 (MQ=255)
                                                                                                                             tCACTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGaaa                     >  1:3793788/1‑150 (MQ=255)
                                                                                                                                cTTACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAg                  <  2:4384821/150‑1 (MQ=255)
                                                                                                                                 ttACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGa                 >  1:3431691/1‑150 (MQ=255)
                                                                                                                                  tACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGaa                <  2:2086033/150‑1 (MQ=255)
                                                                                                                                  tACTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGaa                <  1:1049067/150‑1 (MQ=255)
                                                                                                                                   aCTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGaaa               >  1:3912242/1‑150 (MQ=255)
                                                                                                                                   aCTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGaaa               <  2:2836863/150‑1 (MQ=255)
                                                                                                                                   aCTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGaaa               >  2:2016569/1‑150 (MQ=255)
                                                                                                                                    cTCATCAACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAAt              <  1:1273466/150‑1 (MQ=255)
                                                                                                                                        tcaACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATc          <  1:3629073/150‑1 (MQ=255)
                                                                                                                                         caACTCATTTAATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCg         >  1:4595601/1‑150 (MQ=255)
                                                                                                                                              catttaatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCAtt    >  2:2516698/1‑149 (MQ=255)
                                                                                                                                              catttaatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCAtt    >  1:3303180/1‑149 (MQ=255)
                                                                                                                                               atttaatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCAtta   >  1:1576142/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGAT  >  CP009273/2920954‑2921247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑