Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,921,245 G→T 100% intergenic (+214/‑343) ygdH → / → sdaC UPF0717 family protein/putative serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,921,2450GT99.4% 1158.8 / ‑2.7 357intergenic (+214/‑343)ygdH/sdaCUPF0717 family protein/putative serine transporter
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (184/171);  total (186/171)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 367 total aligned reads displayed.

TTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTATCTTC  >  CP009273/2921098‑2921393
                                                                                                                                                   |                                                                                                                                                    
 ttaatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCattatt                                                                                                                                                   >  2:3255227/4‑150 (MQ=255)
 ttaatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCattatt                                                                                                                                                   >  1:4935366/4‑150 (MQ=255)
  taatttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCAttatta                                                                                                                                                  >  2:1141653/3‑150 (MQ=255)
    atttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAAc                                                                                                                                                >  2:1223509/1‑150 (MQ=255)
      ttGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTa                                                                                                                                              <  2:4563953/150‑1 (MQ=255)
       tGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCAATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaa                                                                                                                                             >  2:1766314/1‑150 (MQ=255)
        gTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaaa                                                                                                                                            >  1:2859638/1‑150 (MQ=255)
         ttATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTaaaa                                                                                                                                           <  2:2121770/150‑1 (MQ=255)
          tataTGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAg                                                                                                                                          <  1:3562787/150‑1 (MQ=255)
           atatTAATGTTTCTTACCACCCTCACGCGACAAATAACATCACAGTTAATATTTCATACAATTTATGTTGCAACGCAAACTTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGt                                                                                                                                         <  1:3694043/150‑1 (MQ=255)
                 aTGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGAt                                                                                                                                   <  2:593174/150‑1 (MQ=255)
                   gTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTGATGTTGCAACGCAAACGTTTCCCTATTGTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTCTTAACTAAAAGTATTGAttt                                                                                                                                 >  1:1927157/1‑150 (MQ=255)
                      tCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGAtttttt                                                                                                                              <  1:2046845/150‑1 (MQ=255)
                         tACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAg                                                                                                                           <  1:2351580/150‑1 (MQ=255)
                           ccaccCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGtt                                                                                                                         <  2:5111422/150‑1 (MQ=255)
                           ccaccCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGtt                                                                                                                         <  2:1108098/150‑1 (MQ=255)
                            caccCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTc                                                                                                                        <  2:1596178/150‑1 (MQ=255)
                              cccTAACTCGAAAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCaa                                                                                                                      <  1:3895877/150‑1 (MQ=255)
                               ccTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAAc                                                                                                                     >  2:4991147/1‑150 (MQ=255)
                                  cACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTa                                                                                                                  <  1:2178148/150‑1 (MQ=255)
                                      cgACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACata                                                                                                              >  1:3394206/1‑150 (MQ=255)
                                        aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatata                                                                                                            <  1:958145/150‑1 (MQ=255)
                                        aCAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACatata                                                                                                            >  1:149412/1‑150 (MQ=255)
                                          aaaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCGGTTCAACCTACAGATAtt                                                                                                          >  1:1175291/1‑150 (MQ=255)
                                           aaTATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTg                                                                                                         >  2:2903290/1‑150 (MQ=255)
                                            atatCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgc                                                                                                        >  2:2844015/1‑150 (MQ=255)
                                             tatCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgcg                                                                                                       <  1:3165394/150‑1 (MQ=255)
                                              atcatcACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgcgc                                                                                                      >  1:515176/1‑150 (MQ=255)
                                              atcatcACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTgcgc                                                                                                      >  2:5291312/1‑150 (MQ=255)
                                                 atcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGcc                                                                                                   <  2:3553442/150‑1 (MQ=255)
                                                 atcaCAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGcc                                                                                                   >  1:1327410/1‑150 (MQ=255)
                                                   cacaGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGcccc                                                                                                 >  2:3005718/1‑150 (MQ=255)
                                                     caGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCgg                                                                                               >  2:1073176/1‑150 (MQ=255)
                                                      aGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGga                                                                                              >  1:1251110/1‑150 (MQ=255)
                                                         tAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGgaaga                                                                                           >  1:381631/1‑150 (MQ=255)
                                                           atatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGaagaag                                                                                         >  2:675209/1‑150 (MQ=255)
                                                           atatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGaagaag                                                                                         >  1:3884323/1‑150 (MQ=255)
                                                            tatGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGt                                                                                        >  2:723273/1‑150 (MQ=255)
                                                             atGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTc                                                                                       >  1:815688/1‑150 (MQ=255)
                                                              tGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCa                                                                                      <  1:4143946/150‑1 (MQ=255)
                                                               gTCATACAATTTATGTTGCAACGCAAACGTGTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAg                                                                                     <  2:562538/150‑1 (MQ=255)
                                                                tCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGa                                                                                    >  1:4780404/1‑150 (MQ=255)
                                                                tCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGa                                                                                    >  1:3284031/1‑150 (MQ=255)
                                                                 cATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGAt                                                                                   <  1:3311886/150‑1 (MQ=255)
                                                                  aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATg                                                                                  <  2:4605541/150‑1 (MQ=255)
                                                                  aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATg                                                                                  <  2:4155209/150‑1 (MQ=255)
                                                                  aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATg                                                                                  >  2:4422765/1‑150 (MQ=255)
                                                                  aTACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTAAGTTCAACCTACATATATTGAGCGCCCCGGAAGAAGTCAGATg                                                                                  >  2:4709854/1‑150 (MQ=255)
                                                                   tACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGt                                                                                 >  2:810959/1‑150 (MQ=255)
                                                                    aCAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTc                                                                                <  1:4714197/150‑1 (MQ=255)
                                                                    aCAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTc                                                                                <  2:3650652/150‑1 (MQ=255)
                                                                        tttATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGttt                                                                            <  1:4330027/150‑1 (MQ=255)
                                                                           aTGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAAt                                                                         <  2:584412/150‑1 (MQ=255)
                                                                           aTGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAAt                                                                         >  1:747643/1‑150 (MQ=255)
                                                                            tGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATg                                                                        >  2:5191811/1‑150 (MQ=255)
                                                                              ttGCAACGCAAACGTTTCCCTATTTTCATAACCCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATggg                                                                      <  1:1627916/150‑1 (MQ=255)
                                                                               tGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGc                                                                     <  2:2497403/150‑1 (MQ=255)
                                                                                gCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCa                                                                    <  2:3929913/150‑1 (MQ=255)
                                                                                    cGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGAGGTCGTTTAATGGGCAAata                                                                >  1:5240980/1‑150 (MQ=255)
                                                                                     gCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAatat                                                               <  2:2743241/150‑1 (MQ=255)
                                                                                      cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCTGTTAAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATAtt                                                              >  1:4459772/1‑150 (MQ=255)
                                                                                      cAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATAtt                                                              >  2:389199/1‑150 (MQ=255)
                                                                                       aaaCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTg                                                             <  1:2449516/150‑1 (MQ=255)
                                                                                       aaaCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAAATGTCGTTTAATGGGCAAATATTg                                                             >  2:1364660/1‑150 (MQ=255)
                                                                                        atcGTTTCACTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGc                                                            <  1:5024981/148‑1 (MQ=255)
                                                                                         aCGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGcc                                                           <  2:5068794/150‑1 (MQ=255)
                                                                                           gTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCt                                                         >  1:5304316/1‑150 (MQ=255)
                                                                                           gTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCt                                                         >  1:2669501/1‑150 (MQ=255)
                                                                                           gTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCt                                                         >  1:602380/1‑150 (MQ=255)
                                                                                             ttCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCTACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTa                                                       >  1:1136897/1‑150 (MQ=255)
                                                                                              tCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaa                                                      >  2:198030/1‑150 (MQ=255)
                                                                                               cccTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTaaa                                                     <  2:3442922/150‑1 (MQ=255)
                                                                                                ccTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAAt                                                    <  2:3793788/150‑1 (MQ=255)
                                                                                                  tATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGTGCAAATATTTCCCTTAAATtc                                                  >  2:2363342/1‑150 (MQ=255)
                                                                                                  tATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAGATtc                                                  <  1:720880/150‑1 (MQ=255)
                                                                                                        cATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCtttt                                            <  2:938859/150‑1 (MQ=255)
                                                                                                         aTAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTa                                           >  2:1731145/1‑150 (MQ=255)
                                                                                                             aCCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACttt                                       <  1:1014190/150‑1 (MQ=255)
                                                                                                                 tATTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAt                                   <  1:1641526/150‑1 (MQ=255)
                                                                                                                  aTTTTATCGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAtt                                  >  2:867008/1‑150 (MQ=255)
                                                                                                                  aTTTTATAGCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGAtt                                  <  2:3194696/150‑1 (MQ=255)
                                                                                                                          gCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGt                          >  1:1761533/1‑150 (MQ=255)
                                                                                                                          gCTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGt                          <  2:1657564/150‑1 (MQ=255)
                                                                                                                           cTATGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTa                         >  2:4857809/1‑150 (MQ=255)
                                                                                                                             aTGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTaaa                       >  1:2962736/1‑150 (MQ=255)
                                                                                                                              tGAAAAAGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAg                      <  1:4397314/150‑1 (MQ=255)
                                                                                                                                   aaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTg                 <  1:4647197/150‑1 (MQ=255)
                                                                                                                                   aaGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTg                 >  2:2213279/1‑150 (MQ=255)
                                                                                                                                    aGAAATTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTgg                >  1:4861058/1‑150 (MQ=255)
                                                                                                                                       aaTTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGAt             >  2:77054/1‑150 (MQ=255)
                                                                                                                                        aTTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa            <  1:5119295/150‑1 (MQ=255)
                                                                                                                                        aTTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa            >  1:3841635/1‑150 (MQ=255)
                                                                                                                                        aTTATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATa            >  1:2812409/1‑150 (MQ=255)
                                                                                                                                          tATCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAat          >  2:3405719/1‑150 (MQ=255)
                                                                                                                                           aTCGCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatc         <  1:3754351/150‑1 (MQ=255)
                                                                                                                                              gCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatctat      <  2:1490647/150‑1 (MQ=255)
                                                                                                                                              gCCATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatctat      <  2:4345983/150‑1 (MQ=255)
                                                                                                                                                cATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatctatct    >  1:666734/1‑150 (MQ=255)
                                                                                                                                                cATTATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAatctatct    >  2:4295191/1‑150 (MQ=255)
                                                                                                                                                  tGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTATCTTc  >  2:2297488/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAACCGTATTTTATCGCTATGAAAAAGAAATTATCGCCATGATTAACTAAAAGTATTGATTTTTTCAGTTCAACCTACATATATTGCGCGCCCCGGAAGAAGTCAGATGTCGTTTAATGGGCAAATATTGCCCTTAAATTCTCTTTTACTTTTGATTTACAGAGTAAAGCGTTGGGATAATCTATCTTC  >  CP009273/2921098‑2921393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑