Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,932,236 G→T 12.4% E480D (GAG→GAT)  fucK → L‑fuculokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,932,2360GT12.4% 1034.0 / 78.4 323E480D (GAG→GAT) fucKL‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base G (119/164);  new base T (16/24);  total (135/188)
Fisher's exact test for biased strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 335 total aligned reads displayed.

GTTCTCGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATCCGCGCTGATG  >  CP009273/2932087‑2932385
                                                                                                                                                     |                                                                                                                                                     
   ctcGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGaa                                                                                                                                                    <  1:4348304/150‑1 (MQ=255)
    tcGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAg                                                                                                                                                   <  2:3657081/150‑1 (MQ=255)
    tcGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAg                                                                                                                                                   <  1:2479564/150‑1 (MQ=255)
      gacgacGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCACACTGACCCTGAATTTCTAGAGGAAgtg                                                                                                                                                 >  1:319612/1‑150 (MQ=255)
       acgacgCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAgtgt                                                                                                                                                <  1:312031/150‑1 (MQ=255)
         gacgCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGa                                                                                                                                              >  2:3924617/1‑150 (MQ=255)
         gacgCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGAACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAATTGTGa                                                                                                                                              >  1:3677935/1‑150 (MQ=255)
          acgCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGaa                                                                                                                                             >  1:1035401/1‑150 (MQ=255)
            gCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAAt                                                                                                                                           <  2:487911/150‑1 (MQ=255)
              cGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGc                                                                                                                                         <  1:340478/150‑1 (MQ=255)
                     aCCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAAc                                                                                                                                  <  2:5263045/150‑1 (MQ=255)
                       cGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACaa                                                                                                                                <  1:3175555/150‑1 (MQ=255)
                        gTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAAt                                                                                                                               >  1:4748263/1‑150 (MQ=255)
                          cGCAGGAGCTGCGCAGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCTCGCACAGATTCATTATCAGTACCGTTATTTCTAACCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATTCTGAAAACAAttt                                                                                                                             >  2:2641532/1‑150 (MQ=255)
                            cAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAATAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCg                                                                                                                           >  1:1813315/1‑150 (MQ=255)
                              ggAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGcc                                                                                                                         <  2:2091917/150‑1 (MQ=255)
                                 gCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGtt                                                                                                                      <  2:1763693/150‑1 (MQ=255)
                                  cTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTa                                                                                                                     >  2:2176125/1‑150 (MQ=255)
                                   tGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTaa                                                                                                                    >  2:2823135/1‑150 (MQ=255)
                                    gcgcTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAAt                                                                                                                   <  2:1653575/150‑1 (MQ=255)
                                       cTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTtc                                                                                                                <  2:2320848/150‑1 (MQ=255)
                                          ttCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTcc                                                                                                             <  1:2653313/150‑1 (MQ=255)
                                          ttCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTcc                                                                                                             <  1:2188441/150‑1 (MQ=255)
                                           tCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTccc                                                                                                            <  2:1700395/150‑1 (MQ=255)
                                           tCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTccc                                                                                                            <  2:87422/150‑1 (MQ=255)
                                            cGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCg                                                                                                           <  2:309268/150‑1 (MQ=255)
                                                 ggTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTa                                                                                                      >  1:4465384/1‑150 (MQ=255)
                                                  gTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTAt                                                                                                     >  1:1402534/1‑150 (MQ=255)
                                                    aTGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTg                                                                                                   >  1:895213/1‑150 (MQ=255)
                                                       gCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGaaa                                                                                                <  1:4623244/150‑1 (MQ=255)
                                                         gTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGt                                                                                              >  2:4417515/1‑150 (MQ=255)
                                                         gTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGt                                                                                              <  1:5277473/150‑1 (MQ=255)
                                                           aGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGc                                                                                            >  1:5169514/1‑150 (MQ=255)
                                                            ggggAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCt                                                                                           >  2:3810979/1‑150 (MQ=255)
                                                               gAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGc                                                                                        <  2:1324568/150‑1 (MQ=255)
                                                               gAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGc                                                                                        <  1:2279386/150‑1 (MQ=255)
                                                                 aTTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCag                                                                                      <  1:1639704/150‑1 (MQ=255)
                                                                 aTTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCag                                                                                      >  2:1773880/1‑150 (MQ=255)
                                                                     aaCAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGAt                                                                                  <  1:4959346/150‑1 (MQ=255)
                                                                     aaCAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGAt                                                                                  <  1:2374413/150‑1 (MQ=255)
                                                                      aCAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATg                                                                                 <  2:3218920/150‑1 (MQ=255)
                                                                       cAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATgg                                                                                <  1:618937/150‑1 (MQ=255)
                                                                        aGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATggg                                                                               >  1:737676/1‑150 (MQ=255)
                                                                         gCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGa                                                                              <  2:573286/150‑1 (MQ=255)
                                                                          cccGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGAc                                                                             <  2:297335/150‑1 (MQ=255)
                                                                          cccGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGAc                                                                             <  1:1194618/150‑1 (MQ=255)
                                                                           ccGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACa                                                                            <  2:1092245/150‑1 (MQ=255)
                                                                                agaagCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGgag                                                                       >  2:2269070/1‑150 (MQ=255)
                                                                                agaagCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGgag                                                                       <  2:4465384/150‑1 (MQ=255)
                                                                                   agCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATg                                                                    <  1:4248603/150‑1 (MQ=255)
                                                                                    gCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGa                                                                   <  1:1087421/150‑1 (MQ=255)
                                                                                     cccGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGaa                                                                  <  2:3558755/150‑1 (MQ=255)
                                                                                       cgcgCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAat                                                                <  2:1160927/150‑1 (MQ=255)
                                                                                         cgcACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAatta                                                              >  2:3397260/1‑150 (MQ=255)
                                                                                         cgcACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAatta                                                              >  1:2009017/1‑150 (MQ=255)
                                                                                         cgcACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTGATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAatta                                                              >  2:3441863/1‑150 (MQ=255)
                                                                                            acaGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTAttt                                                           <  2:4611579/150‑1 (MQ=255)
                                                                                            acaGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTAttt                                                           <  1:1505847/150‑1 (MQ=255)
                                                                                            acaGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTAttt                                                           <  2:3688442/150‑1 (MQ=255)
                                                                                            acaGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTAttt                                                           <  2:3025168/150‑1 (MQ=255)
                                                                                            acaGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTAttt                                                           <  2:2677184/150‑1 (MQ=255)
                                                                                            acaGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTAttt                                                           >  2:2285552/1‑150 (MQ=255)
                                                                                              aGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTAttttt                                                         >  2:3829931/1‑150 (MQ=255)
                                                                                              aGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACATGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGACGTTAATTTCTACCTAACTATTTAAAGTGCTGGCAGAGATGGGACATGGATATGAAATTAttttt                                                         >  2:438695/1‑150 (MQ=255)
                                                                                                aTTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTc                                                       >  1:3376554/1‑150 (MQ=255)
                                                                                                aTTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTc                                                       <  1:3522548/150‑1 (MQ=255)
                                                                                                aTTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTc                                                       >  1:2881340/1‑150 (MQ=255)
                                                                                                 ttCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTcc                                                      <  2:4767814/150‑1 (MQ=255)
                                                                                                  tCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCg                                                     <  1:5068687/150‑1 (MQ=255)
                                                                                                    aTTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGAt                                                   >  1:4199517/1‑150 (MQ=255)
                                                                                                       aTCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCt                                                >  2:3060878/1‑150 (MQ=255)
                                                                                                        tCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATATCGCCGTTAATTTATCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTc                                               >  2:2136980/1‑150 (MQ=255)
                                                                                                        tCAGTACCGTTATTTCTACCCGCAAACTGAAACTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTc                                               <  1:1892757/150‑1 (MQ=255)
                                                                                                         cAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCa                                              <  2:5147365/150‑1 (MQ=255)
                                                                                                         cAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCa                                              <  1:3280169/150‑1 (MQ=255)
                                                                                                           gTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACt                                            <  1:344815/150‑1 (MQ=255)
                                                                                                           gTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACt                                            >  2:1512061/1‑150 (MQ=255)
                                                                                                           gTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCAGATGCTCACt                                            >  1:3801885/1‑150 (MQ=255)
                                                                                                             aCCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACttt                                          <  2:4023635/150‑1 (MQ=255)
                                                                                                               cGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTcc                                        >  1:3618542/1‑150 (MQ=255)
                                                                                                               cGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTcc                                        <  2:2918255/150‑1 (MQ=255)
                                                                                                                   aTTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGcc                                    >  1:5040626/1‑150 (MQ=255)
                                                                                                                      tCTACCCGCAAACTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCAt                                 <  2:2500115/150‑1 (MQ=255)
                                                                                                                       cTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCAtt                                <  2:606225/150‑1 (MQ=255)
                                                                                                                       cTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGAGGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCAtt                                >  2:4193312/1‑150 (MQ=255)
                                                                                                                         aCCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCg                              <  2:5073365/150‑1 (MQ=255)
                                                                                                                         aCCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCg                              <  2:886086/150‑1 (MQ=255)
                                                                                                                           ccGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGAt                            <  1:3929172/150‑1 (MQ=255)
                                                                                                                            cGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATg                           >  1:858200/1‑150 (MQ=255)
                                                                                                                               aaaCTGAACCTGAATTTATAGATGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGa                        >  2:3352543/1‑150 (MQ=255)
                                                                                                                                      aCCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAgg                 <  2:2024124/150‑1 (MQ=255)
                                                                                                                                          gAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGAt             <  1:550161/150‑1 (MQ=255)
                                                                                                                                           aaTTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATc            >  2:5009941/1‑150 (MQ=255)
                                                                                                                                           aaTTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATc            <  1:4429793/150‑1 (MQ=255)
                                                                                                                                            aTTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATcc           <  1:2991810/150‑1 (MQ=255)
                                                                                                                                            aTTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATcc           <  1:283080/150‑1 (MQ=255)
                                                                                                                                             tttATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATCGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATCcg          >  1:4855300/1‑150 (MQ=255)
                                                                                                                                                  agagGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATCCGCGCTg     <  1:4614393/150‑1 (MQ=255)
                                                                                                                                                   gagGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATCCGCGCTGa    >  2:1929122/1‑150 (MQ=255)
                                                                                                                                                    agGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATCCGCGCTGAt   >  1:4045354/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTCTCGACGACGCCGAAACGACCGTCGCAGGAGCTGCGCTGTTCGGTTGGTATGGCGTAGGGGAATTTAACAGCCCGGAAGAAGCCCGCGCACAGATTCATTATCAGTACCGTTATTTCTACCCGCAAACTGAACCTGAATTTATAGAGGAAGTGTGAAATGCTGAAAACAATTTCGCCGTTAATTTCTCCCGAACTATTGAAAGTGCTGGCAGAGATGGGACATGGAGATGAAATTATTTTTTCCGATGCTCACTTTCCCGCCCATTCGATGGGACCGCAGGTGATCCGCGCTGATG  >  CP009273/2932087‑2932385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑