Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,932,835 C→T 100% R37C (CGC→TGC)  fucR → l‑fucose operon activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,932,8350CT98.0% 1138.9 / 8.2 344R37C (CGC→TGC) fucRl‑fucose operon activator
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base T (170/167);  total (172/172)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 351 total aligned reads displayed.

GCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGCGC  >  CP009273/2932688‑2932981
                                                                                                                                                   |                                                                                                                                                  
   tGACGCGGGCGGTTATCGAATGATGGGGTTAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGAt                                                                                                                                               <  1:2280790/150‑1 (MQ=255)
    gACGCTGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTTCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGAtc                                                                                                                                              <  2:4773461/150‑1 (MQ=255)
      cgcgGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGAtctc                                                                                                                                            <  1:1533426/150‑1 (MQ=255)
         gggcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAAt                                                                                                                                         <  2:3296871/150‑1 (MQ=255)
          ggcggTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATg                                                                                                                                        <  2:1283881/150‑1 (MQ=255)
              gTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAAtt                                                                                                                                    >  1:102230/1‑150 (MQ=255)
                tATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTAc                                                                                                                                  >  1:2667397/1‑150 (MQ=255)
                   cGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGa                                                                                                                               >  2:756233/1‑150 (MQ=255)
                   cGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGa                                                                                                                               >  1:556253/1‑150 (MQ=255)
                   cGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCACCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGa                                                                                                                               >  1:1703063/1‑150 (MQ=255)
                    gAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGAc                                                                                                                              >  1:4879006/1‑150 (MQ=255)
                       tgatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCa                                                                                                                           <  2:4233754/150‑1 (MQ=255)
                        gatgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAg                                                                                                                          >  1:3759155/1‑150 (MQ=255)
                         atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAgg                                                                                                                         >  1:2105284/1‑150 (MQ=255)
                         atgGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAgg                                                                                                                         >  1:2659959/1‑150 (MQ=255)
                             ggTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTCAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTaa                                                                                                                     >  2:178287/1‑150 (MQ=255)
                              gTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTaaa                                                                                                                    >  2:4686947/1‑150 (MQ=255)
                                 aaaaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAAtt                                                                                                                 <  1:5232826/150‑1 (MQ=255)
                                   aaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCt                                                                                                               >  2:1017252/1‑150 (MQ=255)
                                   aaaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCt                                                                                                               >  1:3342532/1‑150 (MQ=255)
                                    aaTATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTg                                                                                                              >  1:5138856/1‑150 (MQ=255)
                                      tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTgcg                                                                                                            <  1:1146877/150‑1 (MQ=255)
                                      tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTgcg                                                                                                            <  2:3606249/150‑1 (MQ=255)
                                      tatGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTAGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTgcg                                                                                                            >  2:2621953/1‑150 (MQ=255)
                                       atGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTgcgc                                                                                                           >  2:1326424/1‑150 (MQ=255)
                                       atGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTgcgc                                                                                                           >  1:5023170/1‑150 (MQ=255)
                                        tGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCa                                                                                                          >  2:2499884/1‑150 (MQ=255)
                                              cGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATg                                                                                                    >  2:436180/1‑150 (MQ=255)
                                                 cACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAc                                                                                                 <  2:5138565/150‑1 (MQ=255)
                                                 cACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAc                                                                                                 <  2:4159495/150‑1 (MQ=255)
                                                  aCGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAcg                                                                                                <  1:5123980/150‑1 (MQ=255)
                                                    gCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGAcgcg                                                                                              >  2:1244292/1‑150 (MQ=255)
                                                     ccAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACgcgc                                                                                             >  1:956784/1‑150 (MQ=255)
                                                      cAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCt                                                                                            >  2:4621891/1‑150 (MQ=255)
                                                      cAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCt                                                                                            >  2:392322/1‑150 (MQ=255)
                                                       aGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTa                                                                                           <  1:1866804/150‑1 (MQ=255)
                                                       aGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTa                                                                                           >  2:4841938/1‑150 (MQ=255)
                                                         cAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTaga                                                                                         >  2:1481990/1‑148 (MQ=255)
                                                         cAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTaaa                                                                                         >  1:3595306/1‑150 (MQ=255)
                                                               aTAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATc                                                                                   >  2:538936/1‑150 (MQ=255)
                                                                tAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATcc                                                                                  <  2:3680388/150‑1 (MQ=255)
                                                                tAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATcc                                                                                  <  2:1591035/150‑1 (MQ=255)
                                                                tAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATcc                                                                                  <  2:2382998/150‑1 (MQ=255)
                                                                       ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCa                                                                           >  2:3222676/1‑150 (MQ=255)
                                                                       ccTGCTGCTGAACCATACCAGCCTGACCACGGAAGCACTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGTTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCa                                                                           <  1:2838541/150‑1 (MQ=255)
                                                                         tgctgctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCaaa                                                                         <  2:2666942/150‑1 (MQ=255)
                                                                              ctgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCaa                                                                    <  2:2717302/150‑1 (MQ=255)
                                                                               tgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAg                                                                   >  2:3495672/1‑150 (MQ=255)
                                                                               tgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAg                                                                   <  2:2283101/150‑1 (MQ=255)
                                                                               tgAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAg                                                                   >  2:5314333/1‑150 (MQ=255)
                                                                                 aaCCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGAc                                                                 <  1:2064280/150‑1 (MQ=255)
                                                                                       aCCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGc                                                           >  1:1177485/1‑150 (MQ=255)
                                                                                         cAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGa                                                         <  1:2473447/150‑1 (MQ=255)
                                                                                            ccTGACCACGGAAGCTCTCTCTGAACAGATAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATcc                                                      <  1:4919505/150‑1 (MQ=255)
                                                                                             cTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATccc                                                     <  1:3865334/150‑1 (MQ=255)
                                                                                               gACCACTGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCtt                                                   <  2:1813591/150‑1 (MQ=255)
                                                                                                      gAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATc                                            >  1:634142/1‑150 (MQ=255)
                                                                                                        aGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAg                                          >  2:2194520/1‑150 (MQ=255)
                                                                                                        aGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAg                                          >  1:3022278/1‑150 (MQ=255)
                                                                                                        aGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAg                                          <  1:4829333/150‑1 (MQ=255)
                                                                                                         gCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAgg                                         <  1:3793801/150‑1 (MQ=255)
                                                                                                          ctctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCATTGAATTACAGACGCAGGGTAAAATTCTGAGCAATCATGGACGCGATAAATATATCCACCGTCAAAATCAAGACAGTTGCTATCCCTTTCACATCTGGc                                        >  1:5042688/1‑150 (MQ=255)
                                                                                                           tctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCt                                       <  1:4672499/150‑1 (MQ=255)
                                                                                                            ctctctGAACAGCTATATGTCAGTAAAGAATCCATTAGTTGCGATCTCAATGAATTACAGTCGCAGGGTAAAATTCTGCGCAATCATTGACGCGCTAAATATATCCACCGTCAAAATCAAGTCAGTGGCGATCCCTTTCTCATCAGGCTg                                      <  2:5285100/150‑1 (MQ=255)
                                                                                                            ctctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTg                                      <  1:2134711/150‑1 (MQ=255)
                                                                                                             tctctGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGa                                     >  2:5107484/1‑150 (MQ=255)
                                                                                                                 tGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAg                                 >  1:3426282/1‑150 (MQ=255)
                                                                                                                 tGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAg                                 >  2:4880218/1‑150 (MQ=255)
                                                                                                                  gAACAGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGc                                >  1:778211/1‑150 (MQ=255)
                                                                                                                      aGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAtt                            <  1:1800525/150‑1 (MQ=255)
                                                                                                                      aGCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAtt                            >  1:4765214/1‑150 (MQ=255)
                                                                                                                       gCTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTa                           <  1:4269196/150‑1 (MQ=255)
                                                                                                                        cTAAAGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTAt                          <  2:4004545/150‑1 (MQ=255)
                                                                                                                         tAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATg                         <  2:2926082/150‑1 (MQ=255)
                                                                                                                          aaaGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgc                        >  2:3386896/1‑150 (MQ=255)
                                                                                                                          aaaGGTCAGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATgc                        >  2:2412146/1‑150 (MQ=255)
                                                                                                                                 aGTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATaaa                 <  1:3969333/150‑1 (MQ=255)
                                                                                                                                  gTAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAg                >  2:661546/1‑150 (MQ=255)
                                                                                                                                   tAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGTCGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACTGTGGCGATCCCTTTCACATCTGGCTGAAAAGCCATTATTCGCATAAAGc               <  2:3815329/150‑1 (MQ=255)
                                                                                                                                   tAAAGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGc               <  1:2350774/150‑1 (MQ=255)
                                                                                                                                    aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCa              >  2:5193253/1‑150 (MQ=255)
                                                                                                                                    aaaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCa              >  1:4091651/1‑150 (MQ=255)
                                                                                                                                     aaGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGTAAAAATTCTGCTCAATCATGGACGCGCTAAATATAACCACCGACAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAg             >  1:139844/1‑150 (MQ=255)
                                                                                                                                      aGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCCTTATGCGCATAAAGCAGa            >  1:2947230/1‑150 (MQ=255)
                                                                                                                                      aGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa            >  1:2929454/1‑150 (MQ=255)
                                                                                                                                      aGAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGa            >  1:2804261/1‑150 (MQ=255)
                                                                                                                                       gAAACCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGat           <  2:2060532/150‑1 (MQ=255)
                                                                                                                                        aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGata          >  2:3415885/1‑150 (MQ=255)
                                                                                                                                        aaaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCAGTATGCGCATAAAGCAGata          >  1:1590345/1‑150 (MQ=255)
                                                                                                                                         aaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGatat         >  2:3126987/1‑150 (MQ=255)
                                                                                                                                         aaCCATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGatat         <  1:3495672/150‑1 (MQ=255)
                                                                                                                                           ccATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcg       >  2:1527899/1‑150 (MQ=255)
                                                                                                                                            cATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgc      >  2:4945945/1‑150 (MQ=255)
                                                                                                                                            cATTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgc      <  1:3222676/150‑1 (MQ=255)
                                                                                                                                             aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTCTGCGCATAAAGCAGATATcgcg     >  2:2862191/1‑150 (MQ=255)
                                                                                                                                             aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcg     >  1:1633848/1‑150 (MQ=255)
                                                                                                                                             aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcg     >  1:4477972/1‑150 (MQ=255)
                                                                                                                                             aTTCGTTGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcg     >  2:867515/1‑150 (MQ=255)
                                                                                                                                                cgttgCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcgcgc  >  1:905763/1‑150 (MQ=255)
                                                                                                                                                cgttgCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATcgcgcgc  >  2:192346/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCATGACGCGGGCGGTTATCGAATGATGGGGTGAAAAATATGAAAGCGGCACGCCAGCAAGCGATAGTCGACCTGCTGCTGAACCATACCAGCCTGACCACGGAAGCTCTCTCTGAACAGCTAAAGGTCAGTAAAGAAACCATTCGTCGCGATCTCAATGAATTACAGACGCAGGGTAAAATTCTGCGCAATCATGGACGCGCTAAATATATCCACCGTCAAAATCAAGACAGTGGCGATCCCTTTCACATCAGGCTGAAAAGCCATTATGCGCATAAAGCAGATATCGCGCGC  >  CP009273/2932688‑2932981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑