Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,941,341 G→A 100% L343L (CTC→CTT)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,941,3410GA99.0% 1019.6 / ‑0.7 313L343L (CTC→CTT) amiCN‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (170/139);  total (170/143)
Fisher's exact test for biased strand distribution p-value = 9.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 313 total aligned reads displayed.

CTGGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCAG  >  CP009273/2941194‑2941487
                                                                                                                                                   |                                                                                                                                                  
ctgGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAg                                                                                                                                                  <  1:4605362/150‑1 (MQ=255)
ctgGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAg                                                                                                                                                  >  2:5105336/1‑150 (MQ=255)
   gAAAGTCGACGTTTTCTGTTTACGCTCTTCCTCAACGTTACTGATAATCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTTGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCtt                                                                                                                                               <  1:2663808/150‑1 (MQ=255)
      aGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAtt                                                                                                                                            <  1:1604113/150‑1 (MQ=255)
      aGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAtt                                                                                                                                            >  2:2721223/1‑150 (MQ=255)
          gCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGt                                                                                                                                        <  1:3380761/150‑1 (MQ=255)
           ccGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTa                                                                                                                                       <  1:3709667/150‑1 (MQ=255)
              ttttCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCg                                                                                                                                    <  2:2035841/150‑1 (MQ=255)
                  cAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCttt                                                                                                                                <  1:3738661/150‑1 (MQ=255)
                   aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTa                                                                                                                               <  2:1177041/150‑1 (MQ=255)
                   aGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTa                                                                                                                               <  2:1451366/150‑1 (MQ=255)
                    gTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTAc                                                                                                                              >  2:460714/1‑150 (MQ=255)
                     tttACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTAcc                                                                                                                             <  1:442894/150‑1 (MQ=255)
                     tttACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTAcc                                                                                                                             <  2:5016748/150‑1 (MQ=255)
                      ttACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCa                                                                                                                            <  2:72461/150‑1 (MQ=255)
                      ttACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTAACGCTTTACCa                                                                                                                            >  2:827930/1‑150 (MQ=255)
                      ttACGCTCTTCCTCAACGTTACAGATAAACGCCGTGTCGACCAGAATGGAGGGAATATCTTGTGCCTTTAGTACGGCAAACCCGGCCTGATCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCATTACCa                                                                                                                            >  1:1457604/1‑150 (MQ=255)
                          gCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACt                                                                                                                        <  2:4766158/150‑1 (MQ=255)
                            tctTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTc                                                                                                                      >  2:1070739/1‑150 (MQ=255)
                              ttCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAg                                                                                                                    <  2:4432149/150‑1 (MQ=255)
                                ccTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGc                                                                                                                  <  1:587536/150‑1 (MQ=255)
                                   cAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGt                                                                                                               >  1:4088556/1‑150 (MQ=255)
                                    aaCGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTc                                                                                                              >  2:1280196/1‑150 (MQ=255)
                                    aaCGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTc                                                                                                              >  1:5062636/1‑150 (MQ=255)
                                       gTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGc                                                                                                           >  1:2196766/1‑150 (MQ=255)
                                       gTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGc                                                                                                           >  1:1541926/1‑150 (MQ=255)
                                       gTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGc                                                                                                           >  2:3432880/1‑150 (MQ=255)
                                        ttACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCa                                                                                                          >  2:905922/1‑150 (MQ=255)
                                         tACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCaa                                                                                                         <  2:2663241/150‑1 (MQ=255)
                                          aCTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAAt                                                                                                        <  1:2787566/150‑1 (MQ=255)
                                              aTAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTc                                                                                                    <  2:1651915/150‑1 (MQ=255)
                                              aTAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTc                                                                                                    >  2:4582591/1‑150 (MQ=255)
                                                 aaCGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGc                                                                                                 >  1:1339707/1‑150 (MQ=255)
                                                   cGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGa                                                                                               >  1:741002/1‑150 (MQ=255)
                                                   cGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGa                                                                                               >  2:4968290/1‑150 (MQ=255)
                                                     ccGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCTAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACt                                                                                             <  1:2248089/150‑1 (MQ=255)
                                                     ccGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACt                                                                                             <  2:2115297/150‑1 (MQ=255)
                                                     ccGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACt                                                                                             <  1:3596535/150‑1 (MQ=255)
                                                            gACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAACCCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCat                                                                                      <  2:3841454/150‑1 (MQ=255)
                                                            gACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCACTGGTCAGCGACTGTACCat                                                                                      >  2:1500811/1‑150 (MQ=255)
                                                            gACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCat                                                                                      >  1:3860581/1‑150 (MQ=255)
                                                            gACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCat                                                                                      >  1:4293874/1‑150 (MQ=255)
                                                            gACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCat                                                                                      >  1:2838014/1‑150 (MQ=255)
                                                              ccAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCatat                                                                                    <  1:3112047/150‑1 (MQ=255)
                                                               cAGACTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTTCCATATc                                                                                   >  2:4364915/1‑150 (MQ=255)
                                                                 gAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGa                                                                                 >  2:1527398/1‑150 (MQ=255)
                                                                   aTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAAc                                                                               <  2:617628/150‑1 (MQ=255)
                                                                   aTGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAAc                                                                               >  1:2414736/1‑150 (MQ=255)
                                                                        gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgt                                                                          >  2:3218450/1‑150 (MQ=255)
                                                                        gggAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGgt                                                                          <  2:5209932/150‑1 (MQ=255)
                                                                           aaTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGtgtg                                                                       >  2:87317/1‑150 (MQ=255)
                                                                           aaTATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGtgtg                                                                       <  1:2341972/150‑1 (MQ=255)
                                                                               tCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCg                                                                   <  2:3005878/150‑1 (MQ=255)
                                                                                cTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGa                                                                  >  2:161884/1‑150 (MQ=255)
                                                                                 tGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGAc                                                                 >  1:2863620/1‑150 (MQ=255)
                                                                                       cTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCg                                                           >  1:2422433/1‑150 (MQ=255)
                                                                                         ttAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGt                                                         >  2:1571955/1‑150 (MQ=255)
                                                                                           aGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCa                                                       >  2:3340647/1‑150 (MQ=255)
                                                                                           aGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCa                                                       <  2:2281021/150‑1 (MQ=255)
                                                                                             tACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCAcc                                                     <  1:5288323/150‑1 (MQ=255)
                                                                                               cGGCAAACCCGGCATGTTCTACTTTATTTTTTTGAAGCTTGTTGATTTTACCAAGATTATTCAGTACCGCTTTACCAAACTTCTGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGc                                                   <  1:2076834/150‑1 (MQ=255)
                                                                                                 gCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCtt                                                 <  2:3056902/150‑1 (MQ=255)
                                                                                                  cAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCttt                                                <  2:5804/150‑1 (MQ=255)
                                                                                                   aaaCCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCtttt                                               >  1:3019788/1‑150 (MQ=255)
                                                                                                   aaaCCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCtttt                                               >  1:3634735/1‑150 (MQ=255)
                                                                                                   aaaCCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTAGTCAGTACCGCTTTACCAAACTTCACGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCtttt                                               >  1:643006/1‑150 (MQ=255)
                                                                                                        cGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCa                                          >  2:2378104/1‑150 (MQ=255)
                                                                                                         ggCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCAc                                         <  2:2141716/150‑1 (MQ=255)
                                                                                                         ggCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAAGGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCAc                                         <  1:551643/150‑1 (MQ=255)
                                                                                                         ggCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTACAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCAc                                         <  1:2008311/150‑1 (MQ=255)
                                                                                                          gCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACg                                        >  2:4255430/1‑150 (MQ=255)
                                                                                                           ccTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGc                                       <  2:4074409/150‑1 (MQ=255)
                                                                                                            cTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGcc                                      <  1:5239609/150‑1 (MQ=255)
                                                                                                             tGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGcca                                     >  2:4177950/1‑150 (MQ=255)
                                                                                                              gTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGccac                                    >  2:3571610/1‑150 (MQ=255)
                                                                                                              gTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGAGGTCACCGCTTTTGCTCACGccac                                    >  1:1286161/1‑150 (MQ=255)
                                                                                                               ttCAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGccacc                                   >  2:4301508/1‑150 (MQ=255)
                                                                                                                 cAACTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCaa                                 >  1:3540775/1‑150 (MQ=255)
                                                                                                                  aaCTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAAt                                >  1:4874134/1‑150 (MQ=255)
                                                                                                                  aaCTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAAt                                >  1:2488781/1‑150 (MQ=255)
                                                                                                                  aaCTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAAt                                >  2:2713316/1‑150 (MQ=255)
                                                                                                                    cTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCa                              <  2:47304/150‑1 (MQ=255)
                                                                                                                    cTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCa                              >  1:1797011/1‑150 (MQ=255)
                                                                                                                    cTTGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCa                              <  1:1070739/150‑1 (MQ=255)
                                                                                                                      tGATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAg                            >  1:3943038/1‑150 (MQ=255)
                                                                                                                       gATTTTTATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGt                           <  1:1133866/150‑1 (MQ=255)
                                                                                                                           tttATGCAGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGa                       <  2:2073655/150‑1 (MQ=255)
                                                                                                                            ttATGCTGCTTGTTGATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCTATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAg                      <  1:852651/150‑1 (MQ=255)
                                                                                                                              aTGCAGCTTGTTGAATTAACCAAGCTTATACACTACCGCTTTACCAAACATCAGGCTGACGGCAAAGGTCAGCGACTCTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTCCTCACGCCACCAAACAAGTCCGAGGc                    >  2:1875027/1‑150 (MQ=255)
                                                                                                                                      tgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTggg            >  2:4400205/1‑150 (MQ=255)
                                                                                                                                      tgttgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTggg            <  1:4169408/150‑1 (MQ=255)
                                                                                                                                         tgATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGttt         <  1:2240283/150‑1 (MQ=255)
                                                                                                                                          gATTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtg        <  2:4647304/150‑1 (MQ=255)
                                                                                                                                           aTTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtgt       >  2:2222704/1‑150 (MQ=255)
                                                                                                                                           aTTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtgt       >  1:4673446/1‑150 (MQ=255)
                                                                                                                                           aTTTTACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGC‑TTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTtgtg      >  1:4663923/1‑150 (MQ=255)
                                                                                                                                              ttACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGcc    <  2:2908461/150‑1 (MQ=255)
                                                                                                                                               tACCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACAGGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCa   <  2:3274628/150‑1 (MQ=255)
                                                                                                                                                aCCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCAg  >  2:3744547/1‑150 (MQ=255)
                                                                                                                                                aCCAAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCAg  <  2:1339707/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGAAAGTCGCCGTTTTCAGTTTACGCTCTTCCTCAACGTTACTGATAAACGCCGTTTCGACCAGAATGGAGGGAATATCTGGTGCCTTTAGTACGGCAAACCCGGCCTGTTCAACTTGATTTTTATGCAGCTTGTTGATTTTACCGAGCTTATTCAGTACCGCTTTACCAAACTTCAGGCTGTCGGCAATGGTCAGCGACTGTACCATATCGAACATGGTGTGGTCGACATAGCGGTCACCGCTTTTGCTCACGCCACCAATCAAGTCCGAGGCGTTCTGGGTTTGTGCCAG  >  CP009273/2941194‑2941487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑