Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,988,934 G→T 28.2% pseudogene (186/447 nt) ygeN ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,988,9340GT28.2% 888.0 / 301.7 445pseudogene (186/447 nt)ygeNpseudogene
Reads supporting (aligned to +/- strand):  ref base G (163/156);  new base T (72/53);  total (236/209)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01

Only 100 of 448 total aligned reads displayed.

CAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATAT  >  CP009273/2988786‑2989083
                                                                                                                                                     |                                                                                                                                                     
 aGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGTACATTCCATATTGATTTGTCGAc                                                                                                                                                     >  1:1018398/1‑150 (MQ=255)
 aGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGAc                                                                                                                                                     >  2:5274328/1‑150 (MQ=255)
      agaAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATGCCATATTGATTTGTCGACAAAat                                                                                                                                                >  2:3605793/1‑150 (MQ=255)
         aaCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATg                                                                                                                                             <  1:119100/150‑1 (MQ=255)
         aaCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATg                                                                                                                                             >  1:4084285/1‑150 (MQ=255)
          aCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGa                                                                                                                                            >  2:2823562/1‑150 (MQ=255)
          aCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGa                                                                                                                                            >  2:3529502/1‑150 (MQ=255)
          aCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAAAACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGa                                                                                                                                            <  1:4675772/150‑1 (MQ=255)
           cAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCTGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGAc                                                                                                                                           <  1:4544447/150‑1 (MQ=255)
           cAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGAc                                                                                                                                           >  2:1442582/1‑150 (MQ=255)
            aaTGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGAct                                                                                                                                          >  1:5289419/1‑150 (MQ=255)
            aaTGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGAct                                                                                                                                          >  1:4311914/1‑150 (MQ=255)
              tGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAATACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGActct                                                                                                                                        <  2:3355400/150‑1 (MQ=255)
                  tattaATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACtt                                                                                                                                    <  1:155492/150‑1 (MQ=255)
                  tattaATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACtt                                                                                                                                    >  2:717586/1‑150 (MQ=255)
                     taattaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCaa                                                                                                                                 >  2:3249333/1‑150 (MQ=255)
                     taattaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATACCATATAGATTTGTCTACAAAAAATGACTCTACTTCaa                                                                                                                                 >  1:5250866/1‑150 (MQ=255)
                      aattaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAag                                                                                                                                >  2:636006/1‑150 (MQ=255)
                         taaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAgaga                                                                                                                             >  2:5027952/1‑150 (MQ=255)
                          aaaTATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGAc                                                                                                                            >  2:1580185/1‑150 (MQ=255)
                            atatTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAATACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACtt                                                                                                                          >  1:774411/1‑150 (MQ=255)
                            atatTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACtt                                                                                                                          >  1:3267830/1‑150 (MQ=255)
                             tatTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTa                                                                                                                         <  1:2230087/150‑1 (MQ=255)
                              atTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTAt                                                                                                                        >  1:1291053/1‑150 (MQ=255)
                               ttGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATc                                                                                                                       <  1:3320299/150‑1 (MQ=255)
                                 gCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCtt                                                                                                                     >  1:2727218/1‑150 (MQ=255)
                                  cTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGG‑AAAAAACACAAAACGCTTATCATCATGTAATGTACTATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCttt                                                                                                                    <  2:3965249/150‑1 (MQ=255)
                                   tCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTg                                                                                                                   <  2:2162965/150‑1 (MQ=255)
                                    cacaATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGa                                                                                                                  >  1:1432480/1‑150 (MQ=255)
                                    cacaATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGa                                                                                                                  <  2:162814/150‑1 (MQ=255)
                                     acaATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGaa                                                                                                                 >  1:5054262/1‑150 (MQ=255)
                                     acaATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGaa                                                                                                                 >  1:922126/1‑150 (MQ=255)
                                      caATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGaaa                                                                                                                >  2:4589392/1‑150 (MQ=255)
                                      caATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGaaa                                                                                                                >  1:4770724/1‑150 (MQ=255)
                                       aaTTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGTAAc                                                                                                               >  2:474521/1‑150 (MQ=255)
                                       aaTTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAAc                                                                                                               >  1:112497/1‑150 (MQ=255)
                                        aTTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACt                                                                                                              >  2:5100461/1‑150 (MQ=255)
                                        aTTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACt                                                                                                              >  2:83393/1‑150 (MQ=255)
                                        aTTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACt                                                                                                              <  1:4123325/150‑1 (MQ=255)
                                          tATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTgg                                                                                                            <  2:1327451/150‑1 (MQ=255)
                                           aTCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggt                                                                                                           <  1:209870/150‑1 (MQ=255)
                                             ccACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggtag                                                                                                         <  1:315213/150‑1 (MQ=255)
                                                caAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggtaggta                                                                                                      <  1:681210/150‑1 (MQ=255)
                                                caAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCGTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggtaggta                                                                                                      <  2:1921955/150‑1 (MQ=255)
                                                 aaaTTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGtat                                                                                                     <  2:2298480/150‑1 (MQ=255)
                                                  aaTTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGtata                                                                                                    <  2:4437101/150‑1 (MQ=255)
                                                   aTTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTatat                                                                                                   <  2:2742299/150‑1 (MQ=255)
                                                    ttCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATg                                                                                                  <  1:2797863/150‑1 (MQ=255)
                                                    ttCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATg                                                                                                  <  1:2356061/150‑1 (MQ=255)
                                                    ttCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATg                                                                                                  <  2:1286355/150‑1 (MQ=255)
                                                       cTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGgag                                                                                               <  1:2524970/150‑1 (MQ=255)
                                                          ctgACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGAtt                                                                                            <  2:4638272/148‑1 (MQ=255)
                                                           ggACTAAATTCAGCAATAAATTGACCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGAttt                                                                                           <  1:864833/150‑1 (MQ=255)
                                                            gACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTa                                                                                          >  2:1243138/1‑150 (MQ=255)
                                                             aCTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTAc                                                                                         >  1:179085/1‑150 (MQ=255)
                                                              cTAAATTCAGCAATAAATTGATCGGTAAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTAc                                                                                         >  1:645187/1‑150 (MQ=255)
                                                                 aaTTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGa                                                                                     >  1:3982693/1‑150 (MQ=255)
                                                                    tCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCt                                                                                  >  1:2585674/1‑150 (MQ=255)
                                                                      aGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGa                                                                                >  2:2369773/1‑150 (MQ=255)
                                                                       gCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGat                                                                               <  1:1093807/150‑1 (MQ=255)
                                                                         aaTAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGatat                                                                             <  1:3447319/150‑1 (MQ=255)
                                                                          aTAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATAtt                                                                            <  1:3882017/150‑1 (MQ=255)
                                                                           tAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTg                                                                           <  1:2866641/150‑1 (MQ=255)
                                                                                tGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGt                                                                      >  1:593865/1‑150 (MQ=255)
                                                                                     ggT‑AAAAAACACAACCCGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAAttt                                                                 <  2:4208854/150‑1 (MQ=255)
                                                                                           aaaaCACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTa                                                            >  2:3452039/1‑150 (MQ=255)
                                                                                            aaaCACAAAACGCTTATCATCATGAAATGTAATATGTACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTAc                                                           <  2:929718/150‑1 (MQ=255)
                                                                                                acaAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCaa                                                       <  1:2859368/150‑1 (MQ=255)
                                                                                                acaAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCaa                                                       >  2:5224095/1‑150 (MQ=255)
                                                                                                 caAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAAc                                                      >  2:3692747/1‑150 (MQ=255)
                                                                                                       gCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCaa                                                >  2:3086190/1‑150 (MQ=255)
                                                                                                         ttATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACAGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCaaaa                                              <  2:2011283/150‑1 (MQ=255)
                                                                                                           atcatcATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAAt                                            >  1:78451/1‑150 (MQ=255)
                                                                                                              atcatGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAAtttt                                         >  2:4168442/1‑150 (MQ=255)
                                                                                                                catGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGa                                       >  1:5166126/1‑150 (MQ=255)
                                                                                                                catGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGa                                       >  2:612142/1‑150 (MQ=255)
                                                                                                                 atGAAATGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGAt                                      >  1:3569515/1‑150 (MQ=255)
                                                                                                                 atGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAATTTTTGAt                                      >  2:179482/1‑150 (MQ=255)
                                                                                                                      aTGTAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATaaaaa                                 >  1:2287643/1‑150 (MQ=255)
                                                                                                                       tGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAAc                                >  2:487850/1‑150 (MQ=255)
                                                                                                                         tAATATGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACAt                              <  2:723021/150‑1 (MQ=255)
                                                                                                                          aaTATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATc                             >  2:1128480/1‑150 (MQ=255)
                                                                                                                           atatGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCa                            >  1:2474995/1‑150 (MQ=255)
                                                                                                                             atGGACATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAAc                          >  2:2212201/1‑150 (MQ=255)
                                                                                                                             atGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAAc                          >  1:1911911/1‑150 (MQ=255)
                                                                                                                                  cATTCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAActtctt                     <  1:4586838/150‑1 (MQ=255)
                                                                                                                                     tCCATATTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTg                  <  2:1713204/150‑1 (MQ=255)
                                                                                                                                     tCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTg                  >  2:4904269/1‑150 (MQ=255)
                                                                                                                                       cATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGaa                <  1:4952045/150‑1 (MQ=255)
                                                                                                                                        atatTGATTTGTCTACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAg               >  2:4086714/1‑150 (MQ=255)
                                                                                                                                        atatTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAg               <  2:2862867/150‑1 (MQ=255)
                                                                                                                                         tatTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAgg              >  2:2725041/1‑150 (MQ=255)
                                                                                                                                           ttGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGtt            <  2:268807/150‑1 (MQ=255)
                                                                                                                                              aTTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTa         >  1:3707609/1‑150 (MQ=255)
                                                                                                                                              aTTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTa         >  2:2442849/1‑150 (MQ=255)
                                                                                                                                              aTTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTa         >  1:2202758/1‑150 (MQ=255)
                                                                                                                                              aTTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAAAATCAACTTCTTCTGAAGGTTTTa         >  2:3190320/1‑150 (MQ=255)
                                                                                                                                                  gTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACaa     <  1:2981159/150‑1 (MQ=255)
                                                                                                                                                    cGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAata   <  2:2962652/150‑1 (MQ=255)
                                                                                                                                                     gACAAAATATGACTCTACTACAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAatat  <  1:4375418/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGT‑AAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCTTCTGAAGGTTTTAACAATAT  >  CP009273/2988786‑2989083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑