Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,989,295 C→A 100% pseudogene (85/261 nt) ygeN ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,989,2950CA100.0% 1036.1 / ‑6.7 315pseudogene (85/261 nt)ygeNpseudogene
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (155/159);  total (156/159)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 323 total aligned reads displayed.

AGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACA  >  CP009273/2989148‑2989441
                                                                                                                                                    |                                                                                                                                                  
 gggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTatttattt                                                                                                                                                 >  2:3538876/1‑150 (MQ=255)
   gTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCa                                                                                                                                               >  2:2562732/1‑150 (MQ=255)
     ataAAATTATCCATCTCATAAATAATTCCTTTTGTATAGACATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAAt                                                                                                                                             <  2:3897081/150‑1 (MQ=255)
      taAAATTATCCATCTCATCAATATTGCCTTTTGTATAGACATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATc                                                                                                                                            <  2:3707609/150‑1 (MQ=255)
      taAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATc                                                                                                                                            <  1:5014389/150‑1 (MQ=255)
         aaTTATCCATCTCATCAATAATTCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCAt                                                                                                                                         <  2:2775802/150‑1 (MQ=255)
         aaTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCAt                                                                                                                                         <  1:4350740/150‑1 (MQ=255)
          aTTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATc                                                                                                                                        >  2:3473721/1‑150 (MQ=255)
                cATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTTAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTAAATCAATAAGAAAt                                                                                                                                  >  1:4922355/1‑150 (MQ=255)
                      atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCt                                                                                                                            >  1:5123791/1‑150 (MQ=255)
                       tcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtt                                                                                                                           <  1:3636272/150‑1 (MQ=255)
                       tcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtt                                                                                                                           <  1:259408/150‑1 (MQ=255)
                        caATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCttt                                                                                                                          <  1:2220173/150‑1 (MQ=255)
                         aataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtttt                                                                                                                         <  2:4628643/150‑1 (MQ=255)
                         aataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCtttt                                                                                                                         <  2:5154233/150‑1 (MQ=255)
                          ataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTa                                                                                                                        <  2:2323921/150‑1 (MQ=255)
                          ataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTAAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTa                                                                                                                        <  2:4287581/150‑1 (MQ=255)
                             atGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATc                                                                                                                     >  2:375949/1‑150 (MQ=255)
                             atGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATc                                                                                                                     <  2:4008127/150‑1 (MQ=255)
                              tGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATca                                                                                                                    <  2:102706/150‑1 (MQ=255)
                                 cTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTATTTAAGAGAAACTATTTATTTAAATCCAACAGAAATTTTTCTTTTAATCacaa                                                                                                                 >  1:2851912/1‑150 (MQ=255)
                                  ttttttATATCTATCATAATAACCTTGAATAAATTTTTATTCTCTCTTTTACTCAATACTCTGAA‑TTTTTGCGGTGTTTTCAAGAAGGATTTTTTCTTTAAGTGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCacaac                                                                                                                <  2:2068726/150‑1 (MQ=255)
                                  ttttGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCacaac                                                                                                                <  1:4709967/150‑1 (MQ=255)
                                       tataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAt                                                                                                           <  2:451268/150‑1 (MQ=255)
                                        ataGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAtt                                                                                                          >  2:1479974/1‑150 (MQ=255)
                                         taGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAttt                                                                                                         >  1:3816352/1‑150 (MQ=255)
                                         taGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAttt                                                                                                         >  1:4834927/1‑150 (MQ=255)
                                          aGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTg                                                                                                        >  1:2942637/1‑150 (MQ=255)
                                          aGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACAATTTg                                                                                                        >  1:4758439/1‑150 (MQ=255)
                                                catAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgag                                                                                                  <  2:2646223/150‑1 (MQ=255)
                                                catAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgag                                                                                                  <  2:4224346/150‑1 (MQ=255)
                                                 ataataACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGgaga                                                                                                 >  2:1090720/1‑150 (MQ=255)
                                                   aataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAtt                                                                                               >  1:2805613/1‑150 (MQ=255)
                                                   aataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATAACAACACCATTTGCAGGAGAtt                                                                                               >  2:3253259/1‑150 (MQ=255)
                                                    ataaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAttt                                                                                              >  1:265738/1‑150 (MQ=255)
                                                     taaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAATAAGGATTTTTTCTTTAAGAGAAACTATTTATTTAAATCAATCATAAATTTTTCTTTTAATCACAACAACATTTGCAGGAGAtttt                                                                                             >  2:4956578/1‑150 (MQ=255)
                                                     taaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAtttt                                                                                             <  1:4946123/150‑1 (MQ=255)
                                                      aaCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTa                                                                                            <  2:358765/150‑1 (MQ=255)
                                                       aCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTAt                                                                                           >  1:3521916/1‑150 (MQ=255)
                                                       aCCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTAt                                                                                           >  1:1626633/1‑150 (MQ=255)
                                                          ttGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCaa                                                                                        <  2:793555/150‑1 (MQ=255)
                                                           tGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAg                                                                                       <  2:1840590/150‑1 (MQ=255)
                                                            gAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGc                                                                                      >  1:5286947/1‑150 (MQ=255)
                                                            gAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGc                                                                                      >  1:3950594/1‑150 (MQ=255)
                                                             aaTAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCt                                                                                     <  2:1623975/150‑1 (MQ=255)
                                                                  aTTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtct                                                                                >  1:4669926/1‑150 (MQ=255)
                                                                  aTTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtct                                                                                <  2:3343657/150‑1 (MQ=255)
                                                                   tttttCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGTATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCATAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtctc                                                                               <  2:3357111/150‑1 (MQ=255)
                                                                   tttttCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAtctc                                                                               <  1:3379382/150‑1 (MQ=255)
                                                                       tcttctCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtt                                                                           >  2:2995146/1‑150 (MQ=255)
                                                                       tcttctCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtt                                                                           <  1:642031/150‑1 (MQ=255)
                                                                       tcttctCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtt                                                                           <  1:4482712/150‑1 (MQ=255)
                                                                        cttctCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttt                                                                          <  2:740765/150‑1 (MQ=255)
                                                                         ttctCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTTCAGGAGATTTTATCAAGCTCATCTCTAtttt                                                                         >  1:2252705/1‑150 (MQ=255)
                                                                           ctctctTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttttt                                                                       >  2:1165967/1‑150 (MQ=255)
                                                                           ctctctTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATCTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttttt                                                                       <  1:1990021/150‑1 (MQ=255)
                                                                               ctTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCg                                                                   >  2:33998/1‑150 (MQ=255)
                                                                               ctTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCg                                                                   >  1:1719357/1‑150 (MQ=255)
                                                                                       cAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAAt                                                           <  1:239827/150‑1 (MQ=255)
                                                                                        aaTACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATg                                                          <  1:1043712/150‑1 (MQ=255)
                                                                                         aTACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAAttt                                                         >  2:4709934/1‑148 (MQ=255)
                                                                                           aCTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTaa                                                       <  1:4606882/150‑1 (MQ=255)
                                                                                             tctGAATTTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAAt                                                      <  1:2486837/150‑1 (MQ=255)
                                                                                             tctGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAAtt                                                     <  1:4134829/150‑1 (MQ=255)
                                                                                                 aa‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtctct                                                 <  1:2744796/150‑1 (MQ=255)
                                                                                                  a‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATtctctc                                                <  1:1184242/150‑1 (MQ=255)
                                                                                                    tttttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTctctct                                               >  2:1589607/1‑150 (MQ=255)
                                                                                                     ttttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATCTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTa                                              <  1:90144/150‑1 (MQ=255)
                                                                                                      tttGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTAGCATAAAATGTAATtatctagaa                                             >  1:833152/1‑146 (MQ=255)
                                                                                                          cGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgt                                         <  1:3058780/150‑1 (MQ=255)
                                                                                                           ggtggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtg                                        >  1:2037311/1‑150 (MQ=255)
                                                                                                           ggtggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtg                                        >  2:2010716/1‑150 (MQ=255)
                                                                                                            gtggtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAgtgt                                       >  2:2217066/1‑150 (MQ=255)
                                                                                                              ggtTTCAAGAATGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTg                                     <  1:1889004/150‑1 (MQ=255)
                                                                                                               gtTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGa                                    >  1:4018966/1‑150 (MQ=255)
                                                                                                                tttCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGAt                                   <  1:1090096/150‑1 (MQ=255)
                                                                                                                 ttCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATg                                  >  2:4445198/1‑150 (MQ=255)
                                                                                                                       aagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTg                            >  1:4311404/1‑150 (MQ=255)
                                                                                                                       aagGATTTTTTCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTg                            >  1:4987065/1‑150 (MQ=255)
                                                                                                                            ttttttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAAc                       <  1:769504/150‑1 (MQ=255)
                                                                                                                               tttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGc                    <  2:457686/150‑1 (MQ=255)
                                                                                                                                ttCTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTAGCATAAACTGTAATTCTCTCTAAAAGTGTTGCTGCAAGTGGTAACggag                   >  2:5054653/1‑148 (MQ=255)
                                                                                                                                  cTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATGAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGAt                 >  1:3712157/1‑150 (MQ=255)
                                                                                                                                  cTTTAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGAt                 >  2:4309241/1‑150 (MQ=255)
                                                                                                                                    ttAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTg               >  1:4363047/1‑150 (MQ=255)
                                                                                                                                     tAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt              >  2:4850187/1‑150 (MQ=255)
                                                                                                                                     tAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt              >  2:57992/1‑150 (MQ=255)
                                                                                                                                     tAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt              <  2:837922/150‑1 (MQ=255)
                                                                                                                                     tAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt              >  2:2291646/1‑150 (MQ=255)
                                                                                                                                     tAAGAGAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGt              <  1:5206088/150‑1 (MQ=255)
                                                                                                                                     tAAGAGAAACTATTTATTTCAATCCATCAGAAAATTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATTTAATTCTCTCTAAAAAAGATGATGCAAGAGGTAACGGAGATTGt              >  1:3840627/1‑150 (MQ=255)
                                                                                                                                       agagAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAt            >  2:3307150/1‑150 (MQ=255)
                                                                                                                                       agagAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAt            <  2:3074386/150‑1 (MQ=255)
                                                                                                                                       agagAAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAt            <  1:88836/150‑1 (MQ=255)
                                                                                                                                         agaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt          >  2:1726468/1‑150 (MQ=255)
                                                                                                                                         agaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgt          >  2:1211863/1‑150 (MQ=255)
                                                                                                                                          gaAACTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtgtg         >  1:3216243/1‑150 (MQ=255)
                                                                                                                                           aaaCTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATAAAGCTCATCTCTATTTTTTTTCGAATAAAATGTAATTCTATCTAAAAGTGTTTATGCAAGTGGTAACGGCGATATTAtgttg        >  2:5237166/1‑148 (MQ=255)
                                                                                                                                            aaCTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAAATCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGt       >  1:1412215/1‑150 (MQ=255)
                                                                                                                                              cTATTTATTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTca     <  1:2967935/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAA‑TTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACA  >  CP009273/2989148‑2989441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑