Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,018,544 G→T 100% A279S (GCC→TCC)  xanQ → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,018,5440GT99.1% 1067.7 / ‑0.1 324A279S (GCC→TCC) xanQxanthine permease
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (185/135);  total (189/135)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 324 total aligned reads displayed.

ATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGG  >  CP009273/3018397‑3018690
                                                                                                                                                   |                                                                                                                                                  
aTGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTcc                                                                                                                                                  >  1:4080039/1‑150 (MQ=255)
  ggtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCAc                                                                                                                                                <  1:1658903/150‑1 (MQ=255)
        tttCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAAt                                                                                                                                          <  1:285258/150‑1 (MQ=255)
           cagcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGt                                                                                                                                       >  2:2533245/1‑150 (MQ=255)
            agcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGtt                                                                                                                                      >  2:2869050/1‑150 (MQ=255)
            agcagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGtt                                                                                                                                      >  1:4667978/1‑150 (MQ=255)
               agTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                   <  2:4316672/150‑1 (MQ=255)
               agTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                   <  2:2028962/150‑1 (MQ=255)
               agTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                   >  2:2704471/1‑150 (MQ=255)
               agTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTcc                                                                                                                                   >  1:1359154/1‑150 (MQ=255)
                gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTccc                                                                                                                                  <  2:1018446/150‑1 (MQ=255)
                gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTccc                                                                                                                                  >  1:2148830/1‑150 (MQ=255)
                gTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTccc                                                                                                                                  >  1:5240871/1‑150 (MQ=255)
                   tGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCccgcc                                                                                                                               <  1:2317191/150‑1 (MQ=255)
                      gcAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCgccgcc                                                                                                                            >  2:2148369/1‑150 (MQ=255)
                      gcAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCgccgcc                                                                                                                            >  2:1391968/1‑150 (MQ=255)
                         aTTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCa                                                                                                                         >  1:2179342/1‑150 (MQ=255)
                         aTTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGATTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCa                                                                                                                         >  2:1106441/1‑150 (MQ=255)
                          tttGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCAt                                                                                                                        >  1:576805/1‑150 (MQ=255)
                            tGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTc                                                                                                                      >  2:4087924/1‑150 (MQ=255)
                               cGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAgg                                                                                                                   >  1:1103985/1‑150 (MQ=255)
                                gTTAATCACCATCCCGCATCCGATCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAAGGGTTTCCCGCCGCCCCCATCAggg                                                                                                                  >  2:4891914/1‑150 (MQ=255)
                                  tAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggggg                                                                                                                <  1:1770524/150‑1 (MQ=255)
                                  tAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAggggg                                                                                                                >  2:198656/1‑150 (MQ=255)
                                   aaTCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGa                                                                                                               >  1:2110364/1‑150 (MQ=255)
                                    aTCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGaa                                                                                                              >  1:1001142/1‑150 (MQ=255)
                                     tCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAag                                                                                                             <  1:1896155/150‑1 (MQ=255)
                                          aTCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTAt                                                                                                        <  2:2543644/150‑1 (MQ=255)
                                             ccGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAg                                                                                                     >  1:3751649/1‑150 (MQ=255)
                                               gCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGACGAGTATCAGTc                                                                                                   >  2:5020292/1‑150 (MQ=255)
                                               gCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTc                                                                                                   >  1:2599286/1‑150 (MQ=255)
                                               gCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTc                                                                                                   >  1:4153654/1‑150 (MQ=255)
                                                cATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGACGAGTATCAGTcc                                                                                                  >  2:182897/1‑150 (MQ=255)
                                                cATACGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGAACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACTGCAATGGTTTCCCGCCGCCCCATACAGGGGGAAGAGTATCAGTcc                                                                                                  >  2:4234242/1‑150 (MQ=255)
                                                 aTCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTccc                                                                                                 <  1:4906082/150‑1 (MQ=255)
                                                 aTCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTccc                                                                                                 >  2:1792487/1‑150 (MQ=255)
                                                 aTCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTccc                                                                                                 >  1:4809265/1‑150 (MQ=255)
                                                  tCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCg                                                                                                <  1:3133240/150‑1 (MQ=255)
                                                  tCCGTTCAAATACGGCTTTAGTTTTAGCTACCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCg                                                                                                >  2:4801026/1‑150 (MQ=255)
                                                  tCCGTTCAAATAAGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCg                                                                                                <  2:764823/150‑1 (MQ=255)
                                                     gTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCt                                                                                             >  1:994664/1‑150 (MQ=255)
                                                        cAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGaa                                                                                          >  1:2522739/1‑150 (MQ=255)
                                                          aaTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAg                                                                                        <  1:3645110/150‑1 (MQ=255)
                                                          aaTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAg                                                                                        <  2:4658878/150‑1 (MQ=255)
                                                          aaTACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAg                                                                                        <  2:5516/150‑1 (MQ=255)
                                                             aCGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGGGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcg                                                                                     <  2:692866/150‑1 (MQ=255)
                                                               ggCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcggc                                                                                   >  2:1305407/1‑150 (MQ=255)
                                                               ggCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAggcggc                                                                                   >  1:1203751/1‑150 (MQ=255)
                                                                    tAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGACGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCt                                                                              >  2:2404503/1‑150 (MQ=255)
                                                                    tAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCt                                                                              <  2:3676719/150‑1 (MQ=255)
                                                                    tAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCt                                                                              <  2:823701/150‑1 (MQ=255)
                                                                     aGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGACGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTg                                                                             >  2:385080/1‑150 (MQ=255)
                                                                      gTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTgg                                                                            <  1:163141/150‑1 (MQ=255)
                                                                        tttAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCa                                                                          >  2:1893680/1‑150 (MQ=255)
                                                                         ttAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAg                                                                         <  1:3284119/150‑1 (MQ=255)
                                                                           aGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGAt                                                                       >  1:1563249/1‑150 (MQ=255)
                                                                                  aTCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTgg                                                                >  2:3035000/1‑150 (MQ=255)
                                                                                  aTCAGTTCCTGGTGGTTGGCACGATTTATCTGCTAAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTgg                                                                >  1:410455/1‑150 (MQ=255)
                                                                                     aGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                             >  1:1971877/1‑150 (MQ=255)
                                                                                     aGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGttt                                                             >  2:5141619/1‑150 (MQ=255)
                                                                                         ccTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGt                                                         >  1:4291196/1‑150 (MQ=255)
                                                                                          cTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGtt                                                        <  1:815469/150‑1 (MQ=255)
                                                                                          cTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGtt                                                        >  1:3535135/1‑150 (MQ=255)
                                                                                           tggtggTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTa                                                       >  1:4251284/1‑150 (MQ=255)
                                                                                           tggtggTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTa                                                       >  2:288615/1‑150 (MQ=255)
                                                                                            ggtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTAt                                                      <  2:1869716/150‑1 (MQ=255)
                                                                                             gtggtTGGCACGATTTATCTGCTTAGCGTGCTGGATGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATc                                                     <  2:4361764/150‑1 (MQ=255)
                                                                                             gtggtTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATc                                                     >  1:3933851/1‑150 (MQ=255)
                                                                                                 ttGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCt                                                 >  1:1519921/1‑150 (MQ=255)
                                                                                                  tGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                                >  1:2471826/1‑150 (MQ=255)
                                                                                                  tGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTc                                                >  1:3347021/1‑150 (MQ=255)
                                                                                                   ggCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTcc                                               <  1:18640/150‑1 (MQ=255)
                                                                                                      aCGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCt                                            <  2:4296339/150‑1 (MQ=255)
                                                                                                      aCGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCt                                            >  1:5094801/1‑150 (MQ=255)
                                                                                                       cGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTg                                           >  1:3435974/1‑150 (MQ=255)
                                                                                                            tATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGt                                      >  1:4352862/1‑150 (MQ=255)
                                                                                                             aTCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGTTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGtt                                     >  1:2448946/1‑150 (MQ=255)
                                                                                                             aTCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGtt                                     >  1:4418902/1‑150 (MQ=255)
                                                                                                              tCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTc                                    <  2:3487693/150‑1 (MQ=255)
                                                                                                               cTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCa                                   <  2:3141462/150‑1 (MQ=255)
                                                                                                                  cTTAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTa                                >  1:2057012/1‑150 (MQ=255)
                                                                                                                   ttAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTAc                               <  1:4529054/150‑1 (MQ=255)
                                                                                                                    tAGCGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTAcc                              >  2:2782567/1‑150 (MQ=255)
                                                                                                                       cGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCTATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCGCGGCTGTAAGGCGGCGTTCTGGCTGATGGTCTGGTTTCTGTTATCTCCTCCGCTGTCGTTTCATTACCAtt                           <  1:3057161/150‑1 (MQ=255)
                                                                                                                       cGTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCAtt                           <  2:1627118/150‑1 (MQ=255)
                                                                                                                        gTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTa                          >  2:2993745/1‑150 (MQ=255)
                                                                                                                        gTGCTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTa                          <  1:3705115/150‑1 (MQ=255)
                                                                                                                           cTGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAAcc                       >  1:1107466/1‑150 (MQ=255)
                                                                                                                            tGGAAGCAGTCGGCGATATCACCTCCACGGCAATGGTTACCCGCCGCCCCATTCAGGGGGAAGAGAATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCATCCGCTGTCGGTTCATTACCATTaaaaa                      >  1:727729/1‑147 (MQ=255)
                                                                                                                                 gCAGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGttt                 <  2:1483901/150‑1 (MQ=255)
                                                                                                                                   aGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTgc               >  2:344317/1‑150 (MQ=255)
                                                                                                                                   aGTCGGCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGGTTgc               >  2:4603915/1‑150 (MQ=255)
                                                                                                                                       ggCGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaa           >  2:5155655/1‑150 (MQ=255)
                                                                                                                                         cGATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCaaaa         >  2:2383134/1‑150 (MQ=255)
                                                                                                                                          gATATCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaat        >  2:4905876/1‑150 (MQ=255)
                                                                                                                                             atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaataat     <  1:2648538/150‑1 (MQ=255)
                                                                                                                                             atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaataat     >  1:4705102/1‑150 (MQ=255)
                                                                                                                                             atCACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTATGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAaataat     <  2:2260232/150‑1 (MQ=255)
                                                                                                                                               cACCTCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATgg   >  1:622524/1‑150 (MQ=255)
                                                                                                                                                aCCTCCACGGCAATGGAAACCCGCCGCCCCAATCAGGGGGAAGAGAATCAGACCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGGTTCTGTTATCGCATCCGCTGTCGGTTCATTACCATAAACCACGTTAGCGCAAAATAATggg  >  2:5281087/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCAGGGGGAAGAGTATCAGTCCCGGCTGAAAGGCGGCGTGCTGGCAGATGGTCTGGTTTCTGTTATCGCCTCCGCTGTCGGTTCATTACCATTAACCACGTTTGCGCAAAATAATGGG  >  CP009273/3018397‑3018690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑