BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,062,143	G→A	7.2%	L9F (CTT→TTT)	argO ←	arginine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,062,143	0	G	A	7.2%	1099.6 / 45.9	321	L9F (CTT→TTT)	argO	arginine transporter
Reads supporting (aligned to +/- strand): ref base G (117/179); new base A (6/17); total (124/197)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 323 total aligned reads displayed.

GCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCT  >  CP009273/3061994‑3062291

gCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAg                                                                                                                                                      <  2:3629443/150‑1 (MQ=255)

 ccaccaAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGa                                                                                                                                                     <  1:4431263/150‑1 (MQ=255)

  caccaAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGAc                                                                                                                                                    <  1:1393002/150‑1 (MQ=255)

   accaAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGAcc                                                                                                                                                   <  1:2126607/150‑1 (MQ=255)

    ccaAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCt                                                                                                                                                  <  1:2893442/150‑1 (MQ=255)

     caAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCtt                                                                                                                                                 >  1:4550503/1‑150 (MQ=255)

     caAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCtt                                                                                                                                                 >  2:2540238/1‑150 (MQ=255)

       aaaaTCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGa                                                                                                                                               >  1:1132558/1‑150 (MQ=255)

        aaaTCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGaa                                                                                                                                              <  2:34609/150‑1 (MQ=255)

         aaTCCCGGCGCAAATCAGGACCAACTCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGaaa                                                                                                                                             <  2:1047350/150‑1 (MQ=255)

         aaTCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGaaa                                                                                                                                             >  2:474473/1‑150 (MQ=255)

           tCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGt                                                                                                                                           <  2:2669048/150‑1 (MQ=255)

            cccGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAAACCTTGAAAGta                                                                                                                                          <  2:4431317/150‑1 (MQ=255)

            cccGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAAACCTTGAAAGta                                                                                                                                          <  1:3320440/150‑1 (MQ=255)

             ccGGCGCAAATCTGGACGACCTCGCTGATTGCACAACGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGTAAGtat                                                                                                                                         <  2:2770254/150‑2 (MQ=255)

              cGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAATGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGtaat                                                                                                                                        >  1:2603386/1‑150 (MQ=255)

              cGGCGAAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGtaat                                                                                                                                        <  2:2861039/150‑1 (MQ=255)

                 cgcAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAg                                                                                                                                     >  2:3568307/1‑150 (MQ=255)

                  gcAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGa                                                                                                                                    <  1:2000296/150‑1 (MQ=255)

                  gcAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGa                                                                                                                                    <  1:1612421/150‑1 (MQ=255)

                      aTCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAac                                                                                                                                <  1:2421803/150‑1 (MQ=255)

                         aGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACg                                                                                                                             >  2:326470/1‑150 (MQ=255)

                         aGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACg                                                                                                                             <  2:1258544/150‑1 (MQ=255)

                           gACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGtt                                                                                                                           >  1:1038614/1‑150 (MQ=255)

                            aCCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTg                                                                                                                          <  2:4804893/150‑1 (MQ=255)

                                aaTCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGata                                                                                                                      <  1:763908/150‑1 (MQ=255)

                                aaTCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGata                                                                                                                      >  2:670485/1‑150 (MQ=255)

                                 aTCGCTGATAGCACAAAGAAAGGCAATCATAATGAGGTACTCACGACGAAAGCCCTGATTCATCACAAAAGCATATTGTGGACCGACCGGTAGGAACATAGCCGCCCCAAGTGCAATACCATGAAAGACATAACAAAACACCTTGCatat                                                                                                                     >  2:3915826/1‑150 (MQ=255)

                                    gCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTcc                                                                                                                  <  1:4159403/150‑1 (MQ=255)

                                     cTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCg                                                                                                                 <  2:1452592/150‑1 (MQ=255)

                                     cTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCg                                                                                                                 >  2:4692703/1‑150 (MQ=255)

                                            cacaAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAg                                                                                                          <  1:3376562/150‑1 (MQ=255)

                                             acaAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAACAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGt                                                                                                         <  2:174354/150‑1 (MQ=255)

                                             acaAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGt                                                                                                         >  2:2259226/1‑150 (MQ=255)

                                              caAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTa                                                                                                        <  2:4997347/150‑1 (MQ=255)

                                               aaaGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTAt                                                                                                       <  1:1519380/150‑1 (MQ=255)

                                               aaaGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTAt                                                                                                       >  1:5273134/1‑150 (MQ=255)

                                                aaGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTTCAAAACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATc                                                                                                      <  1:480055/150‑1 (MQ=255)

                                                  gTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTg                                                                                                    <  1:1879339/150‑1 (MQ=255)

                                                  gTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTg                                                                                                    >  1:1816751/1‑150 (MQ=255)

                                                    aaGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGAt                                                                                                  <  1:3776103/150‑1 (MQ=255)

                                                    aaGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATAGTCCGAATTAAGTATCTGAt                                                                                                  <  1:306057/150‑1 (MQ=255)

                                                     aGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATCAAGTATCTGATg                                                                                                 <  1:5138927/150‑1 (MQ=255)

                                                        cAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCag                                                                                              >  2:2365993/1‑150 (MQ=255)

                                                        cAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCag                                                                                              <  1:3793895/150‑1 (MQ=255)

                                                          aTCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCagag                                                                                            <  2:3730533/150‑1 (MQ=255)

                                                          aTCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCagag                                                                                            <  1:470710/150‑1 (MQ=255)

                                                          aTCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAAACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCagag                                                                                            >  1:3598906/1‑150 (MQ=255)

                                                           tCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAgaga                                                                                           >  1:1306442/1‑150 (MQ=255)

                                                             aTAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACt                                                                                         <  2:4352918/150‑1 (MQ=255)

                                                             aTAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACt                                                                                         >  1:877111/1‑150 (MQ=255)

                                                               aaTGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACtat                                                                                       >  1:1095806/1‑150 (MQ=255)

                                                               aaTGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACtat                                                                                       >  2:339240/1‑150 (MQ=255)

                                                                   tgGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATcg                                                                                   <  2:1571558/150‑1 (MQ=255)

                                                                     gTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATcgcg                                                                                 >  2:3996881/1‑150 (MQ=255)

                                                                      tACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCgg                                                                                >  2:4485600/1‑150 (MQ=255)

                                                                        cTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGa                                                                              <  1:4062103/150‑1 (MQ=255)

                                                                         tGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGAt                                                                             >  2:743333/1‑150 (MQ=255)

                                                                              acgTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGtta                                                                        <  2:88478/150‑1 (MQ=255)

                                                                                gTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGttatt                                                                      <  1:299043/150‑1 (MQ=255)

                                                                                 tATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGttatta                                                                     >  2:2606702/1‑150 (MQ=255)

                                                                                   ttcccTGAGTCATCACAATAGCTTTTTGTGGACCGAGCTGTAGGATCATAGCCGCCTGAAGTGCAATACCTTGAAAGTAATAAGAAATTACTTTTGATATTACGTATTAAGTATATGATGCAGTGAATATTTCGCGTGTTTGTTATTAGc                                                                   <  2:5134620/148‑1 (MQ=255)

                                                                                      ccTGATTCATCACAAAAGCATTTTGTGGACCTAGCGTTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGa                                                                <  1:185294/150‑1 (MQ=255)

                                                                                      ccTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGa                                                                >  1:61148/1‑150 (MQ=255)

                                                                                      ccTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGa                                                                >  1:4399735/1‑150 (MQ=255)

                                                                                      ccTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGa                                                                <  1:1682496/150‑1 (MQ=255)

                                                                                          aTTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAAtt                                                            <  2:4102465/150‑1 (MQ=255)

                                                                                           ttCATCACAAACGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACCCGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTg                                                           <  1:4247724/150‑1 (MQ=255)

                                                                                           ttCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTg                                                           >  1:3236198/1‑150 (MQ=255)

                                                                                           gtcatcaCAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTg                                                           <  2:4907478/149‑1 (MQ=255)

                                                                                              atcaCAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGata                                                        >  1:3836230/1‑150 (MQ=255)

                                                                                              atcaCAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGata                                                        >  2:3187381/1‑150 (MQ=255)

                                                                                                 acaAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGataata                                                     <  1:941360/150‑1 (MQ=255)

                                                                                                  caAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAatat                                                    <  1:4727213/150‑1 (MQ=255)

                                                                                                       gCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAAtt                                               <  2:4875637/150‑1 (MQ=255)

                                                                                                          ttttGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGc                                            <  2:1931811/150‑1 (MQ=255)

                                                                                                           tttGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCa                                           <  1:5070253/150‑1 (MQ=255)

                                                                                                           tttGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCa                                           >  2:838523/1‑150 (MQ=255)

                                                                                                             tgtgGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAAt                                         <  1:4854865/150‑1 (MQ=255)

                                                                                                               tgGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAAACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCa                                       >  1:3633679/1‑150 (MQ=255)

                                                                                                                ggACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAg                                      <  2:3943796/150‑1 (MQ=255)

                                                                                                                      aGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAAc                                <  2:1123508/150‑1 (MQ=255)

                                                                                                                        cGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACta                              >  1:3214011/1‑150 (MQ=255)

                                                                                                                        cGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACta                              >  1:3884619/1‑150 (MQ=255)

                                                                                                                         ggTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACtaa                             >  1:3029616/1‑150 (MQ=255)

                                                                                                                         ggTAGGATCATAGCCGCCCCAAGTGCAAAACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATTAGAAAAACtaa                             <  1:2756235/150‑1 (MQ=255)

                                                                                                                            aGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACtaataa                          <  2:1919102/150‑1 (MQ=255)

                                                                                                                                 cATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAagagag                     <  1:1218613/150‑1 (MQ=255)

                                                                                                                                  aTAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAagagaga                    <  2:2101472/150‑1 (MQ=255)

                                                                                                                                    aGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAgg                  <  1:1917412/150‑1 (MQ=255)

                                                                                                                                    aGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAgg                  >  2:3016511/1‑150 (MQ=255)

                                                                                                                                        gccCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCag              <  1:3489449/150‑1 (MQ=255)

                                                                                                                                         ccccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCaga             <  1:3014273/150‑1 (MQ=255)

                                                                                                                                           ccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagaga           <  2:5273134/150‑1 (MQ=255)

                                                                                                                                            cAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagagag          >  1:3990355/1‑150 (MQ=255)

                                                                                                                                             aaGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGt         <  1:4174860/150‑1 (MQ=255)

                                                                                                                                              aGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTATGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGta        <  2:4626984/150‑1 (MQ=255)

                                                                                                                                                tGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatt      >  1:3173021/1‑150 (MQ=255)

                                                                                                                                                 gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGTAATTGATAATATTAATTGGCAATCAGAAAAAATAATAAGAGAGAGGCCAGAGAGtatta     <  1:2284299/150‑1 (MQ=255)

                                                                                                                                                  cAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtattat    <  2:4189210/150‑1 (MQ=255)

GCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAGGATCATAGCCGCCCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCT  >  CP009273/3061994‑3062291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`