BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,086,574	T→A	59.3%	F93L (TTT→TTA)	yqgE →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,086,574	0	T	A	59.3%	212.5 / inf	307	F93L (TTT→TTA)	yqgE	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (63/62); new base A (90/92); total (153/154)
Fisher's exact test for biased strand distribution p-value = 9.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 310 total aligned reads displayed.

AATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTC  >  CP009273/3086428‑3086722

aaTCTCAATATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCt                                                                                                                                                   >  2:5196286/1‑150 (MQ=255)

        aaTTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCAt                                                                                                                                           >  2:1749427/1‑150 (MQ=255)

          ttGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTc                                                                                                                                         <  1:1403616/150‑1 (MQ=255)

          ttGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTc                                                                                                                                         <  1:3519694/150‑1 (MQ=255)

            gAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGc                                                                                                                                       >  2:4001568/1‑150 (MQ=255)

             aaGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCa                                                                                                                                      >  2:3781294/1‑150 (MQ=255)

             aaGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCa                                                                                                                                      <  2:2531911/150‑1 (MQ=255)

              aGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCAt                                                                                                                                     <  1:5275458/150‑1 (MQ=255)

                ggATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCAttt                                                                                                                                   >  1:1107868/1‑150 (MQ=255)

                 gATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTc                                                                                                                                  <  1:4532171/150‑1 (MQ=255)

                  aTTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCa                                                                                                                                 <  1:1080277/150‑1 (MQ=255)

                   ttCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAg                                                                                                                                <  2:4394447/150‑1 (MQ=255)

                     cTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGac                                                                                                                              >  2:2413817/1‑150 (MQ=255)

                         aaaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGacaaca                                                                                                                          <  1:1845933/150‑1 (MQ=255)

                         aaaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGacaaca                                                                                                                          <  2:4294260/150‑1 (MQ=255)

                          aaaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAacac                                                                                                                         >  2:272053/1‑150 (MQ=255)

                           aaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACg                                                                                                                        <  1:1207593/150‑1 (MQ=255)

                           aaGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTAGGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACg                                                                                                                        <  2:2784822/150‑1 (MQ=255)

                                gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAAt                                                                                                                   <  1:956669/150‑1 (MQ=255)

                                gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAAt                                                                                                                   >  1:1196466/1‑150 (MQ=255)

                                gAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAAt                                                                                                                   >  2:4702264/1‑150 (MQ=255)

                                 aaGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATg                                                                                                                  >  1:4257204/1‑150 (MQ=255)

                                    aTCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGAcc                                                                                                               >  1:2988240/1‑150 (MQ=255)

                                       aCGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACt                                                                                                            <  1:4056591/150‑1 (MQ=255)

                                        cGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACtt                                                                                                           <  2:2620227/150‑1 (MQ=255)

                                         gCCGGAGCCGCGTGATGAATCTATCCGGCTGGATAAACCGGTGATGCTCGGCGGTCCGCTGGCGGAAGATCGCGGGTTTATTTTGCATACTCCGCCCGCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAAGGACCACTTc                                                                                                          <  2:1634557/150‑1 (MQ=255)

                                           cGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTccc                                                                                                        >  1:3202436/1‑150 (MQ=255)

                                            ggAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACACCACGGTAATGACCACTTCCcg                                                                                                       >  1:4940522/1‑150 (MQ=255)

                                             gAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCcgc                                                                                                      >  1:4807555/1‑150 (MQ=255)

                                               gCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGa                                                                                                    <  1:2554991/150‑1 (MQ=255)

                                                  gcgTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGAtgt                                                                                                 <  2:824790/150‑1 (MQ=255)

                                                   cgTGATGAATCAATCCGTCAGGAACAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGAGCGCGGGTTTATTTTGCATACACCGCCCTCCAATTTTGCTTCCAGCATTCGCATTACAGACAACACGCTCATGACCACTACCCGCGAtgag                                                                                                >  2:583559/1‑148 (MQ=255)

                                                    gTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGc                                                                                               >  1:1565625/1‑150 (MQ=255)

                                                     tgatgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCt                                                                                              >  1:1010232/1‑150 (MQ=255)

                                                     tgatgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCt                                                                                              >  2:4169122/1‑150 (MQ=255)

                                                      gatgaATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTg                                                                                             <  1:3889153/150‑1 (MQ=255)

                                                           atcaatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAAc                                                                                        >  2:1224653/1‑150 (MQ=255)

                                                              aatcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCt                                                                                     >  2:5303654/1‑150 (MQ=255)

                                                                tcCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCg                                                                                   <  2:4354975/150‑1 (MQ=255)

                                                                tcCGTCTGGATAAACCGGTGATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCg                                                                                   <  2:116112/150‑1 (MQ=255)

                                                                 ccGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCgg                                                                                  <  2:2949866/150‑1 (MQ=255)

                                                                  cGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCAAGCAATCGAATTTCAGACAACACTGTAATGACCACATCCAGCGATGTGCTGGAAACGCTCGGc                                                                                 >  1:1020910/1‑150 (MQ=255)

                                                                   gTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCa                                                                                >  2:4315492/1‑150 (MQ=255)

                                                                      tGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCTCGATGTGCTGGAAACGCTCGGCACCg                                                                             <  2:168093/150‑1 (MQ=255)

                                                                          tAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATaa                                                                         <  1:2841596/150‑1 (MQ=255)

                                                                            aaCCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaac                                                                       >  1:555049/1‑150 (MQ=255)

                                                                            aaCCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaac                                                                       <  1:1753029/150‑1 (MQ=255)

                                                                            aaCCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaac                                                                       <  2:420500/150‑1 (MQ=255)

                                                                             aCCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACCCGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaaca                                                                      <  2:2229332/150‑1 (MQ=255)

                                                                               cGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaacaac                                                                    <  1:5006025/150‑1 (MQ=255)

                                                                               cGGGTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAaacaac                                                                    <  2:4978359/150‑1 (MQ=255)

                                                                                 gTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACAAGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCg                                                                  >  1:303589/1‑150 (MQ=255)

                                                                                       cTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGAc                                                            <  1:2043196/150‑1 (MQ=255)

                                                                                        tCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACg                                                           >  1:4554829/1‑150 (MQ=255)

                                                                                           gcggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAt                                                        <  2:4757244/150‑1 (MQ=255)

                                                                                            cggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTAtt                                                       >  2:103761/1‑150 (MQ=255)

                                                                                             ggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtg                                                      >  2:3300889/1‑150 (MQ=255)

                                                                                             ggTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtg                                                      <  1:2711565/150‑1 (MQ=255)

                                                                                               tCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATtggt                                                    <  2:3591830/150‑1 (MQ=255)

                                                                                                     ggctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGctct                                              <  1:5242742/150‑1 (MQ=255)

                                                                                                      gctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTg                                             <  2:3657986/150‑1 (MQ=255)

                                                                                                       ctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                            <  1:4351988/150‑1 (MQ=255)

                                                                                                       ctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                            <  2:2064615/150‑1 (MQ=255)

                                                                                                       ctgAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTgg                                            <  1:2944728/150‑1 (MQ=255)

                                                                                                          aaGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGtt                                         >  2:2997533/1‑150 (MQ=255)

                                                                                                            gATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTAt                                       <  1:3665583/150‑1 (MQ=255)

                                                                                                            gATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTAt                                       <  2:3164269/150‑1 (MQ=255)

                                                                                                            gATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTAt                                       >  2:3353890/1‑150 (MQ=255)

                                                                                                              tCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCCGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGc                                     >  1:48643/1‑150 (MQ=255)

                                                                                                              tCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGc                                     <  2:2369852/150‑1 (MQ=255)

                                                                                                                 cgGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctc                                  <  1:2502658/150‑1 (MQ=255)

                                                                                                                   ggTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATACACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                                >  2:2286584/1‑150 (MQ=255)

                                                                                                                   ggTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTCTTGGTGGCTCTGGGTTATGcctcct                                >  2:2002487/1‑150 (MQ=255)

                                                                                                                   ggTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                                >  2:1262886/1‑150 (MQ=255)

                                                                                                                   ggTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                                <  1:708256/150‑1 (MQ=255)

                                                                                                                   ggTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                                <  2:4098384/150‑1 (MQ=255)

                                                                                                                   ggTTTATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGcctcct                                >  2:4905813/1‑150 (MQ=255)

                                                                                                                      ttATTTTGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTggg                             >  2:3514512/1‑150 (MQ=255)

                                                                                                                         ttttGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGgaga                          >  2:4325482/1‑150 (MQ=255)

                                                                                                                            tGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAg                       >  2:3460169/1‑150 (MQ=255)

                                                                                                                            tGCATACTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAg                       <  1:2787424/150‑1 (MQ=255)

                                                                                                                              cATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGt                     >  1:644978/1‑150 (MQ=255)

                                                                                                                               aTACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTc                    <  1:336284/150‑1 (MQ=255)

                                                                                                                                 aCTCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCaa                  <  1:4574658/150‑1 (MQ=255)

                                                                                                                                   tCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACt                <  1:1393774/150‑1 (MQ=255)

                                                                                                                                   tCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACt                <  1:2209192/150‑1 (MQ=255)

                                                                                                                                   tCCGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACt                <  1:88526/150‑1 (MQ=255)

                                                                                                                                    ccGCCCTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTg               <  1:1304403/150‑1 (MQ=255)

                                                                                                                                     cGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTgg              >  1:3991854/1‑150 (MQ=255)

                                                                                                                                       cccTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGaa            >  1:165017/1‑150 (MQ=255)

                                                                                                                                        ccTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAAc           >  1:3836403/1‑150 (MQ=255)

                                                                                                                                         cTCCAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACa          <  1:2329114/150‑1 (MQ=255)

                                                                                                                                           ccAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAg        <  2:1115132/150‑1 (MQ=255)

                                                                                                                                            cAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGa       >  1:286047/1‑150 (MQ=255)

                                                                                                                                            cAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGa       >  1:92324/1‑150 (MQ=255)

                                                                                                                                            cAATTTAGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGa       >  1:4202444/1‑150 (MQ=255)

                                                                                                                                             aaTTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGaa      >  2:1347836/1‑150 (MQ=255)

                                                                                                                                              aTTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGaaa     >  2:3235319/1‑150 (MQ=255)

                                                                                                                                               tttaGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAAt    <  2:5125418/150‑1 (MQ=255)

                                                                                                                                                 ttGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATtc  >  2:4664454/1‑150 (MQ=255)

AATCTCAAAATTGAAGGGATTCTGGAAAAGCTGAAGATCACGCCGGAGCCGCGTGATGAATCAATCCGTCTGGATAAACCGGTTATGCTCGGCGGTCCGCTGGCTGAAGATCGCGGGTTTATTTTGCATACTCCGCCCTCCAATTTTGCTTCCAGCATTCGCATTTCAGACAACACGGTAATGACCACTTCCCGCGATGTGCTGGAAACGCTCGGCACCGATAAACAACCGTCTGACGTATTGGTGGCTCTGGGTTATGCCTCCTGGGAGAAAGGTCAACTGGAACAAGAAATTC  >  CP009273/3086428‑3086722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`