Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,213 T→A 100% A753A (GCA→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,2130TA99.1% 1051.0 / ‑1.5 318A753A (GCA→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (133/182);  total (133/185)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 318 total aligned reads displayed.

ACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAG  >  CP009273/3110067‑3110359
                                                                                                                                                  |                                                                                                                                                  
  gtCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCGGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCAt                                                                                                                                               <  2:3819163/149‑1 (MQ=255)
   tCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATc                                                                                                                                              >  1:4590166/1‑150 (MQ=255)
    ccccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCg                                                                                                                                             <  2:1557976/150‑1 (MQ=255)
     cccTGTCTTACTATCGCTGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCACATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGa                                                                                                                                            <  2:2307406/150‑1 (MQ=255)
       cTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACa                                                                                                                                          >  2:1437322/1‑150 (MQ=255)
       cTGTCTTACTAGCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATAGTTATTTACTACCGACTTGTTCAGAGCCATCGACa                                                                                                                                          >  1:4972116/1‑150 (MQ=255)
        tGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAg                                                                                                                                         <  2:2108928/150‑1 (MQ=255)
        tGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAg                                                                                                                                         >  2:1328110/1‑150 (MQ=255)
         gTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGa                                                                                                                                        >  2:871036/1‑150 (MQ=255)
          tCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGAc                                                                                                                                       >  2:4132497/1‑150 (MQ=255)
           cTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGAcc                                                                                                                                      >  1:4606367/1‑150 (MQ=255)
           cTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGAcc                                                                                                                                      <  2:3236816/150‑1 (MQ=255)
              aCTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGc                                                                                                                                   <  2:2401606/150‑1 (MQ=255)
              aCTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGc                                                                                                                                   <  1:2990459/150‑1 (MQ=255)
               cTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCACTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACAGACTTGTTCAGAGCCATCGACAGACCTGCg                                                                                                                                  >  2:2064977/1‑150 (MQ=255)
                 aTCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACCGACCTGCGGc                                                                                                                                >  1:3021683/1‑150 (MQ=255)
                 aTCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGc                                                                                                                                >  1:5292604/1‑150 (MQ=255)
                    gATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATc                                                                                                                             >  1:582958/1‑150 (MQ=255)
                     aTGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATcc                                                                                                                            >  1:4818949/1‑150 (MQ=255)
                        gtgtACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAAc                                                                                                                         <  2:2694346/150‑1 (MQ=255)
                           tACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGa                                                                                                                      <  1:1003738/150‑1 (MQ=255)
                             cGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACg                                                                                                                    >  2:4017430/1‑150 (MQ=255)
                              ggCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGc                                                                                                                   <  2:4380357/150‑1 (MQ=255)
                              ggCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGc                                                                                                                   >  1:3665095/1‑150 (MQ=255)
                                 aGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGcaca                                                                                                                <  2:3449934/150‑1 (MQ=255)
                                    gcACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACaaaa                                                                                                             >  1:2363480/1‑150 (MQ=255)
                                      aCCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAcc                                                                                                           <  2:4489797/150‑1 (MQ=255)
                                          tCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAc                                                                                                       <  2:1821433/150‑1 (MQ=255)
                                           cTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                      <  2:4802658/150‑1 (MQ=255)
                                           cTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcg                                                                                                      <  1:1753308/150‑1 (MQ=255)
                                                gCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctc                                                                                                 <  1:5179560/150‑1 (MQ=255)
                                                  aGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                               <  2:4865708/150‑1 (MQ=255)
                                                  aGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctc                                                                                               <  1:2160198/150‑1 (MQ=255)
                                                   ggATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGctctct                                                                                              >  2:4637739/1‑150 (MQ=255)
                                                    gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                             <  1:2811884/150‑1 (MQ=255)
                                                    gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                             <  2:2013032/150‑1 (MQ=255)
                                                    gATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                             >  1:1198120/1‑150 (MQ=255)
                                                     aTAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTc                                                                                            <  1:2411965/150‑1 (MQ=255)
                                                            tCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGa                                                                                     >  1:1106984/1‑150 (MQ=255)
                                                             cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                    >  2:61386/1‑150 (MQ=255)
                                                              aTAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACTGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAtt                                                                                   <  2:4677724/150‑1 (MQ=255)
                                                               tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTg                                                                                  <  2:2485074/150‑1 (MQ=255)
                                                                  aCCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtc                                                                               <  1:469787/150‑1 (MQ=255)
                                                                   cccAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtca                                                                              <  2:3915636/150‑1 (MQ=255)
                                                                     cAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                            >  1:2339991/1‑150 (MQ=255)
                                                                     cAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                            >  1:2916835/1‑150 (MQ=255)
                                                                      aaaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatca                                                                           >  1:3076774/1‑150 (MQ=255)
                                                                        aTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACg                                                                         <  2:2278558/150‑1 (MQ=255)
                                                                          gCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtt                                                                       <  2:338213/150‑1 (MQ=255)
                                                                                tGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCAt                                                                 >  1:2473287/1‑150 (MQ=255)
                                                                                 gcgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATg                                                                <  1:4471198/150‑1 (MQ=255)
                                                                                 gcgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATg                                                                <  1:5082517/150‑1 (MQ=255)
                                                                                   gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAt                                                              >  1:4355529/1‑150 (MQ=255)
                                                                                       tgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGc                                                          >  1:635039/1‑150 (MQ=255)
                                                                                               aaCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATcc                                                  >  1:5001061/1‑150 (MQ=255)
                                                                                               aaCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATcc                                                  >  2:5091134/1‑150 (MQ=255)
                                                                                                aCGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCa                                                 <  2:459006/150‑1 (MQ=255)
                                                                                                  gcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCAcc                                               <  1:1298775/150‑1 (MQ=255)
                                                                                                    ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                             <  1:1437322/150‑1 (MQ=255)
                                                                                                    ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                             >  2:1369467/1‑150 (MQ=255)
                                                                                                    ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                             <  2:2574275/150‑1 (MQ=255)
                                                                                                    ggTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                             <  1:3235779/150‑1 (MQ=255)
                                                                                                     gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                            >  1:3952249/1‑150 (MQ=255)
                                                                                                     gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCttt                                            >  1:2552442/1‑150 (MQ=255)
                                                                                                        cGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                         <  1:416896/150‑1 (MQ=255)
                                                                                                        cGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                         <  2:4976044/150‑1 (MQ=255)
                                                                                                        cGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAAAAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                         <  1:1032059/150‑1 (MQ=255)
                                                                                                          gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCa                                       >  2:288104/1‑150 (MQ=255)
                                                                                                            tACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGa                                     >  2:5299969/1‑150 (MQ=255)
                                                                                                            tACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGa                                     <  1:1655447/150‑1 (MQ=255)
                                                                                                             aCCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGAt                                    >  2:2986591/1‑150 (MQ=255)
                                                                                                                cTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAgg                                 >  1:486058/1‑150 (MQ=255)
                                                                                                                cTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAgg                                 >  1:2094448/1‑150 (MQ=255)
                                                                                                                 ttGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGc                                <  1:782182/150‑1 (MQ=255)
                                                                                                                 ttGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGc                                <  1:4243003/150‑1 (MQ=255)
                                                                                                                  tGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCg                               <  2:4912471/150‑1 (MQ=255)
                                                                                                                    cGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGAt                             <  2:3349006/150‑1 (MQ=255)
                                                                                                                     ggATCGTTATTTACAACCGACTTTTTCAGAGCAATCGACAGACCTGCGTCAAAAAACAGACGCACAAAACCAGACGCGCTCTCTACCTGAAGAATGATCATCACGATTTCCAAGATCAGCAACGAACCACATATCTTCAGAAAGTCGAta                            >  2:1866698/1‑149 (MQ=255)
                                                                                                                      gATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCa                           >  2:1301362/1‑150 (MQ=255)
                                                                                                                      gATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCa                           >  2:1596611/1‑150 (MQ=255)
                                                                                                                         cGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAt                        <  2:879266/150‑1 (MQ=255)
                                                                                                                         cGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAt                        <  1:734179/150‑1 (MQ=255)
                                                                                                                          gTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATc                       >  1:1941957/1‑150 (MQ=255)
                                                                                                                          gTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACAGATCCACCTTTGTTCAGATAGGCGATCAGATc                       >  2:1645208/1‑150 (MQ=255)
                                                                                                                           ttATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCg                      <  2:3580369/150‑1 (MQ=255)
                                                                                                                           ttATTTACGACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCg                      <  2:3702276/150‑1 (MQ=255)
                                                                                                                                tactccCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTcac                 <  2:709394/150‑1 (MQ=255)
                                                                                                                                   tacCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATc              <  1:2753582/150‑1 (MQ=255)
                                                                                                                                     ccGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCct            >  1:4078364/1‑150 (MQ=255)
                                                                                                                                     ccGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCct            <  1:3502637/150‑1 (MQ=255)
                                                                                                                                      cGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctg           >  2:5118365/1‑150 (MQ=255)
                                                                                                                                       gACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgc          >  1:467461/1‑150 (MQ=255)
                                                                                                                                       gACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgc          <  1:4132497/150‑1 (MQ=255)
                                                                                                                                        aCTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgct         <  2:4229200/150‑1 (MQ=255)
                                                                                                                                        aCTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgct         <  2:2363365/150‑1 (MQ=255)
                                                                                                                                         cTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgctg        >  2:309975/1‑150 (MQ=255)
                                                                                                                                              tCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCa   <  1:3309131/150‑1 (MQ=255)
                                                                                                                                              tCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCa   >  1:1537506/1‑150 (MQ=255)
                                                                                                                                               cAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAg  <  2:2148760/150‑1 (MQ=255)
                                                                                                                                               cAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAg  >  1:547980/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAG  >  CP009273/3110067‑3110359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑