BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,116,446	A→C	30.3%	L240R (CTG→CGG)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,116,446	0	A	C	30.3%	333.7 / inf	330	L240R (CTG→CGG)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base A (125/105); new base C (47/53); total (172/158)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 333 total aligned reads displayed.

GGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAAC  >  CP009273/3116297‑3116595

 gTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAg                                                                                                                                                      <  1:2965056/150‑1 (MQ=255)

   tGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCt                                                                                                                                                    <  1:3910411/150‑1 (MQ=255)

     cGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCa                                                                                                                                                  <  2:4361801/150‑1 (MQ=255)

       gTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAat                                                                                                                                                >  2:4646499/1‑150 (MQ=255)

        tACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAata                                                                                                                                               >  2:3465967/1‑150 (MQ=255)

        tACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAata                                                                                                                                               >  1:2600356/1‑150 (MQ=255)

         aCAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAatat                                                                                                                                              <  1:3741211/150‑1 (MQ=255)

         aCAGCAGGAGTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAagat                                                                                                                                              <  2:2256705/150‑1 (MQ=255)

          cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                             <  2:2667031/150‑1 (MQ=255)

            gcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCa                                                                                                                                           >  2:5280301/1‑150 (MQ=255)

              agGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAgg                                                                                                                                         >  2:902877/1‑150 (MQ=255)

               ggATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGc                                                                                                                                        <  1:4499498/150‑1 (MQ=255)

               ggATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGc                                                                                                                                        >  2:3911128/1‑150 (MQ=255)

                 aTTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGTAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCg                                                                                                                                      >  1:2141768/1‑150 (MQ=255)

                 aTTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCg                                                                                                                                      >  1:782325/1‑150 (MQ=255)

                 aTTTTATCTTCCGCATCAACCGCCGCGACCGAATCTACGCAGTCGAGAATGGTACTGATAGCAGCTTCTACGATAACATCGTTGATGTGCGCCGGAGCGTCTATGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCg                                                                                                                                      >  1:4436998/1‑150 (MQ=255)

                  ttttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGt                                                                                                                                     <  2:3416585/150‑1 (MQ=255)

                   tttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGtt                                                                                                                                    <  1:4194870/150‑1 (MQ=255)

                      aTCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTAtt                                                                                                                                 <  2:2420784/150‑1 (MQ=255)

                      aTCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTAtt                                                                                                                                 <  1:1197615/150‑1 (MQ=255)

                      aTCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTAtt                                                                                                                                 >  1:1883333/1‑150 (MQ=255)

                       tCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTAttt                                                                                                                                >  2:30492/1‑150 (MQ=255)

                       tCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTAttt                                                                                                                                >  2:1536731/1‑150 (MQ=255)

                         ttCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTAttttt                                                                                                                              <  2:3193318/150‑1 (MQ=255)

                          tCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTc                                                                                                                             >  2:2017945/1‑150 (MQ=255)

                             gCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGc                                                                                                                          >  1:4841497/1‑150 (MQ=255)

                             gCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGc                                                                                                                          >  1:1955827/1‑150 (MQ=255)

                               aTCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCaa                                                                                                                        >  2:1717604/1‑150 (MQ=255)

                                  aaCCGCCGCGANCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAAt                                                                                                                     >  1:2767045/1‑150 (MQ=255)

                                   aCCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATg                                                                                                                    <  2:1620107/150‑1 (MQ=255)

                                      gccgcGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAg                                                                                                                 >  1:1518118/1‑150 (MQ=255)

                                      gccgcGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTAGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGTAATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAg                                                                                                                 <  2:1171719/150‑1 (MQ=255)

                                       ccgcGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAgg                                                                                                                <  2:4325078/150‑1 (MQ=255)

                                          cgACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCg                                                                                                             >  2:550260/1‑150 (MQ=255)

                                             ccGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGgcg                                                                                                          <  1:1660728/150‑1 (MQ=255)

                                                aaTCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAg                                                                                                       >  2:4427972/1‑150 (MQ=255)

                                                 aTCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGc                                                                                                      >  2:209179/1‑150 (MQ=255)

                                                         aGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGc                                                                                              >  2:2715255/1‑150 (MQ=255)

                                                          gTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATTCAGGTCGGCGCAGCGGCAACGcc                                                                                             >  1:562298/1‑150 (MQ=255)

                                                            cGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCAc                                                                                           <  2:3609872/150‑1 (MQ=255)

                                                            cGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCAc                                                                                           <  2:3120730/150‑1 (MQ=255)

                                                                 aTGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTaa                                                                                      >  2:4088474/1‑150 (MQ=255)

                                                                   ggTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAcc                                                                                    >  1:3142372/1‑150 (MQ=255)

                                                                    gTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCg                                                                                   >  2:4950890/1‑150 (MQ=255)

                                                                        tGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACaa                                                                               >  1:2326486/1‑150 (MQ=255)

                                                                          aTAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAAc                                                                             >  2:3715714/1‑150 (MQ=255)

                                                                          aTAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAAc                                                                             <  2:2828057/150‑1 (MQ=255)

                                                                          aTAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAAc                                                                             <  1:300442/150‑1 (MQ=255)

                                                                          aTAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAAc                                                                             <  1:406661/150‑1 (MQ=255)

                                                                            agcagcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACtg                                                                           <  2:1452843/150‑1 (MQ=255)

                                                                            agcagcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACtg                                                                           >  1:4366595/1‑150 (MQ=255)

                                                                               agcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGc                                                                        >  1:4959137/1‑150 (MQ=255)

                                                                               agcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGc                                                                        >  2:4527140/1‑150 (MQ=255)

                                                                                   tCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCCCGGTAACCGACAAACTGTGCTGGa                                                                    >  1:1009307/1‑150 (MQ=255)

                                                                                    cgacgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAg                                                                   >  1:4146975/1‑150 (MQ=255)

                                                                                     gacgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGt                                                                  <  1:3211723/150‑1 (MQ=255)

                                                                                     gacgaTAAAATCGTTGATGTGCGCCGGATCGTCTTTGACAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGACGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGt                                                                  <  1:594648/150‑1 (MQ=255)

                                                                                        gaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACg                                                               <  2:2932749/150‑1 (MQ=255)

                                                                                        gaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACg                                                               >  2:2533842/1‑150 (MQ=255)

                                                                                            aCATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAAc                                                           >  2:2299373/1‑150 (MQ=255)

                                                                                              aTCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGt                                                         >  1:338054/1‑150 (MQ=255)

                                                                                               tCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTg                                                        <  1:927618/150‑1 (MQ=255)

                                                                                                cGTTGATGTGCGCCGGATCGTCTTTGCCAATCAGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTgg                                                       <  2:857337/150‑1 (MQ=255)

                                                                                                  ttGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGtt                                                     >  1:4772496/1‑150 (MQ=255)

                                                                                                  ttGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGtt                                                     <  2:1261103/150‑1 (MQ=255)

                                                                                                   tGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACAGTGCTGGAGTACGTAACGGGGttt                                                    >  2:2414924/1‑150 (MQ=255)

                                                                                                      tgtgCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCtt                                                 <  1:1390545/150‑1 (MQ=255)

                                                                                                          cgcCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTAcc                                             <  1:643870/150‑1 (MQ=255)

                                                                                                           gcCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCa                                            <  1:803935/150‑1 (MQ=255)

                                                                                                             cGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGGGTACGTAACGTGGTTTCTTTACCAtt                                          <  2:481402/150‑1 (MQ=255)

                                                                                                              ggATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCAttt                                         >  1:3644722/1‑150 (MQ=255)

                                                                                                                aTCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCAttttt                                       <  1:4878781/150‑1 (MQ=255)

                                                                                                                 tCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTc                                      <  1:168151/150‑1 (MQ=255)

                                                                                                                  cGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCa                                     >  2:4107630/1‑150 (MQ=255)

                                                                                                                     cTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                                  <  1:1833911/150‑1 (MQ=255)

                                                                                                                     cTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                                  <  2:1883333/150‑1 (MQ=255)

                                                                                                                     cTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                                  <  1:4030559/150‑1 (MQ=255)

                                                                                                                     cTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                                  >  2:2216797/1‑150 (MQ=255)

                                                                                                                       ttGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTTCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTgg                                <  2:5016335/150‑1 (MQ=255)

                                                                                                                         gCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGAt                              <  1:4540492/150‑1 (MQ=255)

                                                                                                                            aaTCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCg                           >  2:4766237/1‑150 (MQ=255)

                                                                                                                            aaTCCGCCCATTGGCATCGATTAGCAGATCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTAAGTAACGTGGTTTCTTTACCATTTTTAAACTGGATGCg                           >  2:2135085/1‑150 (MQ=255)

                                                                                                                                cGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAAt                       <  2:2369668/150‑1 (MQ=255)

                                                                                                                                 gCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAAtt                      <  1:1434341/150‑1 (MQ=255)

                                                                                                                                   ccATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGt                    >  1:2039607/1‑150 (MQ=255)

                                                                                                                                    cATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGtt                   >  2:4750915/1‑150 (MQ=255)

                                                                                                                                    cATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTGTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGtt                   <  2:4894388/150‑1 (MQ=255)

                                                                                                                                    cATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGtt                   >  1:5109069/1‑150 (MQ=255)

                                                                                                                                     aTTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGttt                  >  2:4459515/1‑150 (MQ=255)

                                                                                                                                     aTTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGttt                  >  2:1437452/1‑150 (MQ=255)

                                                                                                                                     aTTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGttt                  >  2:402591/1‑150 (MQ=255)

                                                                                                                                       tGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTAt                <  1:3467319/150‑1 (MQ=255)

                                                                                                                                       tGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTAt                <  2:5157859/150‑1 (MQ=255)

                                                                                                                                       tGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTAt                <  1:5236966/150‑1 (MQ=255)

                                                                                                                                         gCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCa              <  1:4239630/150‑1 (MQ=255)

                                                                                                                                            tcgacgagCAGCTCAATATGCAGGCCGTTATTTTTCAGCACAATGCAGGTCTGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTTGAGTACGTAACGTGGGTTCTTTACCATTTTTCCACTGGATGCGTAATTGTTTATCAacc           <  1:5038939/143‑1 (MQ=255)

                                                                                                                                             cGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAacca          >  2:4202644/1‑150 (MQ=255)

                                                                                                                                              gATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAaccac         <  2:782325/150‑1 (MQ=255)

                                                                                                                                                   gCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTaa    >  1:1750980/1‑150 (MQ=255)

                                                                                                                                                    cAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTAAACTGGATGCGTAATTGTTTATCAACCACCTTaaa   >  2:3340508/1‑150 (MQ=255)

GGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAAC  >  CP009273/3116297‑3116595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`