Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,122,356 G→T 100% L242F (TTG→TTT)  glcC → glycolate‑inducible glc operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,122,3560GT100.0% 1053.2 / NA 318L242F (TTG→TTT) glcCglycolate‑inducible glc operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (126/192);  total (126/192)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 318 total aligned reads displayed.

GCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAC  >  CP009273/3122209‑3122501
                                                                                                                                                   |                                                                                                                                                 
  aaaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATCTCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                               >  2:4403726/1‑150 (MQ=255)
     aaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCt                                                                                                                                            <  1:3834432/150‑1 (MQ=255)
      aaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTc                                                                                                                                           <  2:4291119/150‑1 (MQ=255)
          cAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggcg                                                                                                                                       >  1:3986355/1‑150 (MQ=255)
           aGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggcgg                                                                                                                                      >  2:3839486/1‑150 (MQ=255)
            gatcgatcGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGt                                                                                                                                     >  1:2404200/1‑150 (MQ=255)
             atcgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATGTTATTCGCTCGGCGGTg                                                                                                                                    >  2:4112743/1‑150 (MQ=255)
                gatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                                 >  1:3701905/1‑150 (MQ=255)
                gatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                                 >  1:3665902/1‑150 (MQ=255)
                gatcgCCAGCATGCGAGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                                 >  2:289399/1‑150 (MQ=255)
                 atcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGc                                                                                                                                >  2:2948854/1‑150 (MQ=255)
                  tcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCt                                                                                                                               <  1:2618082/150‑1 (MQ=255)
                    gCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTgg                                                                                                                             >  2:4692254/1‑150 (MQ=255)
                    gCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTgg                                                                                                                             >  1:4045373/1‑150 (MQ=255)
                      cAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGACGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGgag                                                                                                                           >  1:5240622/1‑150 (MQ=255)
                      cAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGgag                                                                                                                           >  2:5120074/1‑150 (MQ=255)
                        gCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGAt                                                                                                                         <  2:3752343/150‑1 (MQ=255)
                        gCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGAt                                                                                                                         <  1:1129579/150‑1 (MQ=255)
                          aTGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGa                                                                                                                       <  2:3518735/150‑1 (MQ=255)
                          aTGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGa                                                                                                                       >  2:2665536/1‑150 (MQ=255)
                            gagcccaTCTACAACGCGGTGTTGCAGAGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGAGCCGACCGAGAAAAAGAAAATCCACGATATCGATCTGGAAGGCAACCATATTATTCGCTCGGCGGTGCCGCTGGAGATGAAc                                                                                                                     >  2:4567230/7‑150 (MQ=255)
                             cgcgGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAAcc                                                                                                                    <  2:660892/150‑1 (MQ=255)
                             cgcgGATCTACAACGCGGTGGTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAAcc                                                                                                                    <  1:3641554/150‑1 (MQ=255)
                              gcgGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCt                                                                                                                   <  1:1673061/150‑1 (MQ=255)
                                ggATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGa                                                                                                                 >  1:5071221/1‑150 (MQ=255)
                                  aTCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGt                                                                                                               <  1:4844311/150‑1 (MQ=255)
                                   tCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGGAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGtt                                                                                                              <  2:4781832/150‑1 (MQ=255)
                                    cTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTGTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTa                                                                                                             >  2:3320905/1‑150 (MQ=255)
                                     tACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAg                                                                                                            >  1:5267686/1‑150 (MQ=255)
                                     tACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAg                                                                                                            >  1:1530098/1‑150 (MQ=255)
                                      aCAACGCGGTGTTGCAGCGGCTGCCGCAGGTCGCCCAGCGCGCAGCTCGCGATCATGTGCGGTCCGTGAAAAAGAATCTCCTCGATATCGAGCTGGAAGGCCTCCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGc                                                                                                           <  1:2123012/150‑1 (MQ=255)
                                        aaCGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTg                                                                                                         <  1:1385927/150‑1 (MQ=255)
                                        aaCGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTg                                                                                                         <  2:352269/150‑1 (MQ=255)
                                            cgGTGTTGCAGCGGCTGCCTCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAt                                                                                                     <  2:4865441/150‑1 (MQ=255)
                                            cgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAt                                                                                                     <  1:640746/150‑1 (MQ=255)
                                             ggTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAtt                                                                                                    <  1:4307591/150‑1 (MQ=255)
                                                gtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTaaa                                                                                                 <  1:1936679/150‑1 (MQ=255)
                                                       cGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGCGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGTGATGAACCTGTGTTAGCTGGTATTAAATCTGCtt                                                                                          <  1:3496294/150‑1 (MQ=255)
                                                           tGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCa                                                                                      <  2:2446095/150‑1 (MQ=255)
                                                           tGCCGCACGTCGCCCAGCGCGCAGCACGCGATCAGGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCa                                                                                      <  2:885232/150‑1 (MQ=255)
                                                              cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                                                                                   <  2:3375691/150‑1 (MQ=255)
                                                              cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                                                                                   <  1:2908630/150‑1 (MQ=255)
                                                              cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                                                                                   >  1:3628166/1‑150 (MQ=255)
                                                              cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                                                                                   <  1:4535793/150‑1 (MQ=255)
                                                               gCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACa                                                                                  <  1:374390/150‑1 (MQ=255)
                                                                cACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACaa                                                                                 >  1:3436319/1‑150 (MQ=255)
                                                                 aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                                <  2:347649/150‑1 (MQ=255)
                                                                 aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                                <  1:5187717/150‑1 (MQ=255)
                                                                  cGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATc                                                                               <  2:4707906/150‑1 (MQ=255)
                                                                   gTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCg                                                                              <  1:1163592/150‑1 (MQ=255)
                                                                   gTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCg                                                                              >  1:1915149/1‑150 (MQ=255)
                                                                     cGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                            <  1:6850/150‑1 (MQ=255)
                                                                       cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTaa                                                                          >  1:3861859/1‑150 (MQ=255)
                                                                        ccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAAc                                                                         <  1:4400775/150‑1 (MQ=255)
                                                                         cAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACg                                                                        <  1:2814277/150‑1 (MQ=255)
                                                                         cAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACg                                                                        <  2:575352/150‑1 (MQ=255)
                                                                           gcgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCt                                                                      <  1:2778394/150‑1 (MQ=255)
                                                                                cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTTGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTAc                                                                 <  1:2665536/150‑1 (MQ=255)
                                                                                    aCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                             <  2:5169818/150‑1 (MQ=255)
                                                                                    aCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCt                                                             <  1:469202/150‑1 (MQ=255)
                                                                                      gcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTcc                                                           <  2:3025039/150‑1 (MQ=255)
                                                                                         aTCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                        >  2:1051422/1‑150 (MQ=255)
                                                                                           cATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcccc                                                      <  2:1573428/150‑1 (MQ=255)
                                                                                            aTGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                     <  2:170385/150‑1 (MQ=255)
                                                                                            aTGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                     >  1:4543113/1‑150 (MQ=255)
                                                                                            aTGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGATAGCTGGTATTAAATCTGATTTTCATACAATCGGTCACGCTTGTACGGAACCGCCCCa                                                     >  1:4712575/1‑150 (MQ=255)
                                                                                                gCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGc                                                 <  2:2323107/150‑1 (MQ=255)
                                                                                                      cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                           <  2:2172277/150‑1 (MQ=255)
                                                                                                        tGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGc                                         >  1:1209587/1‑150 (MQ=255)
                                                                                                          aaaaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt                                       <  1:2237466/150‑1 (MQ=255)
                                                                                                           aaaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                      >  1:4489262/1‑150 (MQ=255)
                                                                                                             aaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGtt                                    <  1:4594239/150‑1 (MQ=255)
                                                                                                             aaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAGTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGtt                                    >  2:1778366/1‑150 (MQ=255)
                                                                                                               gAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                  <  2:3169231/150‑1 (MQ=255)
                                                                                                               gAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                  <  1:102019/150‑1 (MQ=255)
                                                                                                               gAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                  <  2:2070056/150‑1 (MQ=255)
                                                                                                               gAATATCCACGATAGCGAGATGGAAGGCCACAATTTTATTCGCTCGGCGGTGCCTCTGGATATGAACCTGAGTTAGCTGTTATTAAATCTGCTTTTAATAAAATCGGTAACGCTTGTACGGCTCAGCCCCAATTCGTTCCAGCATGTTAt                                  <  2:2774721/150‑1 (MQ=255)
                                                                                                                aaTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                 >  1:3079769/1‑150 (MQ=255)
                                                                                                                aaTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAtt                                 <  1:4098081/150‑1 (MQ=255)
                                                                                                                 aTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                                <  1:2979191/150‑1 (MQ=255)
                                                                                                                 aTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGATCCAGCATGTTATTc                                >  2:4078546/1‑150 (MQ=255)
                                                                                                                   ctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                              <  1:955530/150‑1 (MQ=255)
                                                                                                                   ctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                              <  1:810364/150‑1 (MQ=255)
                                                                                                                    tcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg                             <  2:81527/150‑1 (MQ=255)
                                                                                                                    tcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg                             <  1:3161598/150‑1 (MQ=255)
                                                                                                                          atatCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                       >  2:3309936/1‑150 (MQ=255)
                                                                                                                          atatCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                       >  2:3106925/1‑150 (MQ=255)
                                                                                                                           tatCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAt                      <  1:3254651/150‑1 (MQ=255)
                                                                                                                           tatCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAAAGCGTTCCAGCATGTTATTCATGCCTGTAt                      >  2:3621650/1‑150 (MQ=255)
                                                                                                                            atCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt                     >  2:2537516/1‑150 (MQ=255)
                                                                                                                              cGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGATAGCTGGTATTAAATCTCCTTTTCATACAATCGGTACCGCTTGTACGGCTCCGCCCCAAAGCGTTCCAGCATGTTATTCATGCCTGTATTgg                   >  2:4232932/1‑150 (MQ=255)
                                                                                                                                 gCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGttt                <  1:1760455/150‑1 (MQ=255)
                                                                                                                                  cTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTTGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               <  2:5014445/150‑1 (MQ=255)
                                                                                                                                   tGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCg              <  2:4002132/150‑1 (MQ=255)
                                                                                                                                    ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             >  1:4238950/1‑150 (MQ=255)
                                                                                                                                    ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             <  1:3046823/150‑1 (MQ=255)
                                                                                                                                      aaGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTGTTGGTTTCGAgg           >  2:739989/1‑150 (MQ=255)
                                                                                                                                       aGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGa          <  2:5267686/150‑1 (MQ=255)
                                                                                                                                       aGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGGTATTCATGCCTGTATGGGTTTCGAGGa          >  2:1248540/1‑150 (MQ=255)
                                                                                                                                           caccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATccc      >  1:4324876/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAC  >  CP009273/3122209‑3122501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑