Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,184,211 C→A 100% S74Y (TCT→TAT)  yqiI → fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,184,2110CA99.0% 1229.2 / 1.8 383S74Y (TCT→TAT) yqiIfimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (175/202);  total (177/206)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 383 total aligned reads displayed.

TGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAG  >  CP009273/3184065‑3184357
                                                                                                                                                  |                                                                                                                                                  
tGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATcc                                                                                                                                                 <  1:4619806/150‑1 (MQ=255)
    gAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGga                                                                                                                                             >  1:2509478/1‑150 (MQ=255)
         tGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGagaaga                                                                                                                                        >  2:4044581/1‑150 (MQ=255)
             ggTGGAAATACCGTTGTCAGTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATAc                                                                                                                                    <  1:5004322/150‑1 (MQ=255)
                ggAAATACCGTTGTCATTGGTTACTTACCAAGAATATCTATCCCCAGCGATTGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCa                                                                                                                                 <  2:4654094/150‑1 (MQ=255)
                ggAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCa                                                                                                                                 <  1:2394850/150‑1 (MQ=255)
                    aTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTg                                                                                                                             <  1:3711019/150‑1 (MQ=255)
                        cGTTGTCATTGGTTACTTACCAAGAATATCTATCCCCATCGATGTTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATg                                                                                                                         <  1:4015908/150‑1 (MQ=255)
                             tCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCaa                                                                                                                    <  2:711197/150‑1 (MQ=255)
                               aTTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAat                                                                                                                  >  2:2154268/1‑150 (MQ=255)
                                ttGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAata                                                                                                                 >  1:497783/1‑150 (MQ=255)
                                  ggTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAAtata                                                                                                               >  1:3799525/1‑150 (MQ=255)
                                    ttacttacCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAat                                                                                                             <  2:4598399/150‑1 (MQ=255)
                                      aCGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAatga                                                                                                           <  2:5215063/150‑1 (MQ=255)
                                         tACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGAtgt                                                                                                        <  1:363782/150‑1 (MQ=255)
                                           ccAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGAtgttg                                                                                                      <  2:1142278/150‑1 (MQ=255)
                                           ccAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGAtgttg                                                                                                      >  1:2454426/1‑150 (MQ=255)
                                              aGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTa                                                                                                   >  2:2789195/1‑150 (MQ=255)
                                                aaTCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGc                                                                                                 <  2:60967/150‑1 (MQ=255)
                                                 aTCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCa                                                                                                <  1:602767/150‑1 (MQ=255)
                                                   ctctATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTTGCAAAGCAGTGAATATTTTATGAATGTTTTATGTAATAATGCACTAACAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGTCAAATATAATTATGTTGTTataatc                                                                                              >  1:4945696/1‑145 (MQ=255)
                                                        tCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTc                                                                                         >  2:1185383/1‑150 (MQ=255)
                                                         ccccAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCa                                                                                        <  2:5169926/150‑1 (MQ=255)
                                                          cccAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCaa                                                                                       >  2:5096127/1‑150 (MQ=255)
                                                           ccAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAg                                                                                      <  1:3481109/150‑1 (MQ=255)
                                                               cGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACtt                                                                                  >  1:1411792/1‑150 (MQ=255)
                                                               cGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACtt                                                                                  >  1:4659958/1‑150 (MQ=255)
                                                                gATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACttt                                                                                 <  1:2729369/150‑1 (MQ=255)
                                                                 aTGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACtttt                                                                                >  1:4324173/1‑150 (MQ=255)
                                                                 aTGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACtttt                                                                                <  1:3415646/150‑1 (MQ=255)
                                                                   ggTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtat                                                                              >  1:1031750/1‑150 (MQ=255)
                                                                   ggTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtat                                                                              <  1:3275441/150‑1 (MQ=255)
                                                                      aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAAt                                                                           >  2:2334029/1‑150 (MQ=255)
                                                                      aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAAt                                                                           <  1:975429/150‑1 (MQ=255)
                                                                       aaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATc                                                                          >  2:2357067/1‑150 (MQ=255)
                                                                       aaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATc                                                                          >  1:2072698/1‑150 (MQ=255)
                                                                         aaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaa                                                                        >  1:3098654/1‑150 (MQ=255)
                                                                            ggTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAAc                                                                     >  1:2312042/1‑150 (MQ=255)
                                                                             gTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAAct                                                                    >  1:1606684/1‑150 (MQ=255)
                                                                             gTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAAct                                                                    >  2:2705682/1‑150 (MQ=255)
                                                                             gTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAAct                                                                    <  2:1398792/150‑1 (MQ=255)
                                                                              tGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActc                                                                   >  2:4960956/1‑150 (MQ=255)
                                                                               gATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                  <  2:1600119/150‑1 (MQ=255)
                                                                               gATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                  <  1:1968104/150‑1 (MQ=255)
                                                                                 tAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTta                                                                <  2:2547708/150‑1 (MQ=255)
                                                                                 tAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTta                                                                <  1:1507683/150‑1 (MQ=255)
                                                                                 tAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTta                                                                >  2:4339342/1‑150 (MQ=255)
                                                                                  aaaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtat                                                               <  2:293591/150‑1 (MQ=255)
                                                                                  aaaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtat                                                               >  1:1706357/1‑150 (MQ=255)
                                                                                    aaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATAc                                                             >  2:1890192/1‑150 (MQ=255)
                                                                                    aaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATAc                                                             <  2:1625107/150‑1 (MQ=255)
                                                                                     aTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACt                                                            <  1:146878/150‑1 (MQ=255)
                                                                                     aTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACt                                                            >  1:3761409/1‑150 (MQ=255)
                                                                                       cTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACttt                                                          <  2:4318350/150‑1 (MQ=255)
                                                                                          gCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCgg                                                       <  2:55082/150‑1 (MQ=255)
                                                                                                 aGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACt                                                >  2:2484254/1‑150 (MQ=255)
                                                                                                  gTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACtt                                               <  2:3918674/150‑1 (MQ=255)
                                                                                                    gAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTtat                                             <  2:4969050/150‑1 (MQ=255)
                                                                                                     aaTATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTtata                                            >  1:1820562/1‑150 (MQ=255)
                                                                                                       tatTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAAt                                          <  2:4901661/150‑1 (MQ=255)
                                                                                                         ttttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgg                                        <  1:4471166/150‑1 (MQ=255)
                                                                                                         ttttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgg                                        >  2:3206311/1‑150 (MQ=255)
                                                                                                           ttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgggg                                      >  1:2997991/1‑150 (MQ=255)
                                                                                                           ttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgggg                                      >  2:832224/1‑150 (MQ=255)
                                                                                                           ttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCAGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACATTCGGTGTAACTTATAATgggg                                      >  2:146207/1‑150 (MQ=255)
                                                                                                            tATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGt                                     <  2:3789747/150‑1 (MQ=255)
                                                                                                             aTGAATGTTTTCTGTAATAATGCACTACCAGGCCCATTTCCAGGAGAAGATTACCCATCTGCATGGGCAAATATAATGATGTTGTTAGAATCAGGTCAAGTCTTTTATAATCAAAACTCGTATACTTTCGGTGTATCTTATAATGGGGtt                                    <  2:4980086/150‑1 (MQ=255)
                                                                                                             aTGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                    >  2:3241138/1‑150 (MQ=255)
                                                                                                              tGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTg                                   <  2:2850828/150‑1 (MQ=255)
                                                                                                               gAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                  <  2:2242503/150‑1 (MQ=255)
                                                                                                               gAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                  <  2:1542070/150‑1 (MQ=255)
                                                                                                                aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAt                                 <  2:4249369/150‑1 (MQ=255)
                                                                                                                aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAt                                 >  1:918587/1‑150 (MQ=255)
                                                                                                                aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAt                                 >  2:478716/1‑150 (MQ=255)
                                                                                                                 aTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAtt                                <  1:4234992/150‑1 (MQ=255)
                                                                                                                   gTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTAt                              >  1:1017500/1‑150 (MQ=255)
                                                                                                                      ttCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGAt                           >  2:1307151/1‑150 (MQ=255)
                                                                                                                       tCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGAtt                          <  1:483860/150‑1 (MQ=255)
                                                                                                                         tGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCt                        <  2:4387540/150‑1 (MQ=255)
                                                                                                                           taataaTGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAAGGGGGTTGATTATGATTCTAc                      >  2:3003357/1‑150 (MQ=255)
                                                                                                                            aataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTCTACTTTCGGTGTAACTTATCATGGGGTTGATTATGATTCTACa                     >  2:4997023/1‑150 (MQ=255)
                                                                                                                            aataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACa                     >  1:2813462/1‑150 (MQ=255)
                                                                                                                             ataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAt                    >  1:2675411/1‑150 (MQ=255)
                                                                                                                             ataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAt                    >  1:2623975/1‑150 (MQ=255)
                                                                                                                              taatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtc                   >  2:2629755/1‑150 (MQ=255)
                                                                                                                               aatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                  >  1:114194/1‑150 (MQ=255)
                                                                                                                               aatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                  >  1:1194574/1‑150 (MQ=255)
                                                                                                                               aatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                  >  1:5252275/1‑150 (MQ=255)
                                                                                                                               aatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                  <  2:3454058/150‑1 (MQ=255)
                                                                                                                                 tGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTcc                <  1:2363805/150‑1 (MQ=255)
                                                                                                                                 tGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTcc                <  1:223605/150‑1 (MQ=255)
                                                                                                                                   cACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATACTGGGGTTGATTATGATTCTACATCTCCAc              >  2:3146064/1‑150 (MQ=255)
                                                                                                                                   cACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCAc              >  2:1909134/1‑150 (MQ=255)
                                                                                                                                    aCTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACt             <  1:682976/150‑1 (MQ=255)
                                                                                                                                    aCTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACt             <  2:4137246/150‑1 (MQ=255)
                                                                                                                                     cTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCAAcaa            >  2:3624144/1‑148 (MQ=255)
                                                                                                                                         cAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTa        >  1:2045069/1‑150 (MQ=255)
                                                                                                                                             cccATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgc    >  1:4412687/1‑150 (MQ=255)
                                                                                                                                              ccATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgca   <  1:5081484/150‑1 (MQ=255)
                                                                                                                                               cATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTgcag  >  1:454664/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAG  >  CP009273/3184065‑3184357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑