Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,193,329 G→T 5.8% G332G (GGC→GGA)  ygiF ← inorganic triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,193,3290GT 5.8% 906.9 / 7.1 289G332G (GGC→GGA) ygiFinorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base G (113/150);  new base T (24/2);  total (137/152)
Fisher's exact test for biased strand distribution p-value = 1.12e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

AGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCA  >  CP009273/3193180‑3193477
                                                                                                                                                     |                                                                                                                                                    
   aaCAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGccc                                                                                                                                                   >  1:2578945/1‑150 (MQ=255)
    aCAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCt                                                                                                                                                  <  2:1380890/150‑1 (MQ=255)
     cAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTg                                                                                                                                                 <  1:2995671/150‑1 (MQ=255)
     cAGTATTGAGTCAATATCAAGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGTCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGATGATTTTGCCCTg                                                                                                                                                 >  2:1590791/1‑150 (MQ=255)
      aGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTgc                                                                                                                                                <  1:3661161/150‑1 (MQ=255)
         aTTGAGTCAATATCACTCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCt                                                                                                                                             <  2:2722227/150‑1 (MQ=255)
          ttGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCtt                                                                                                                                            <  2:2284889/150‑1 (MQ=255)
                cAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCAt                                                                                                                                      <  1:4562477/150‑1 (MQ=255)
                  atatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGACCTGCGCTTTGGCATCt                                                                                                                                    >  1:3433463/1‑150 (MQ=255)
                   tatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCTGCGCTTTGGCCTCTa                                                                                                                                   >  1:3737358/1‑150 (MQ=255)
                   tatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCTGCGCTTTGGCATCTa                                                                                                                                   >  1:649416/1‑150 (MQ=255)
                   tatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTa                                                                                                                                   >  1:716128/1‑150 (MQ=255)
                   tatCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTa                                                                                                                                   <  2:901768/150‑1 (MQ=255)
                    atCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaa                                                                                                                                  <  1:1854636/150‑1 (MQ=255)
                      cACGCGTCAGGTGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGTGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaaaa                                                                                                                                <  2:1913265/150‑1 (MQ=255)
                       aCGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaaaaa                                                                                                                               >  1:2209354/1‑150 (MQ=255)
                        cgcgTCAGGCGTGGCGACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAAt                                                                                                                              <  2:4769531/150‑1 (MQ=255)
                        cgcgTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAAt                                                                                                                              <  1:4422957/150‑1 (MQ=255)
                          cgTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCGGCGCTTTGGCATCTAAAAATgg                                                                                                                            >  1:624740/1‑150 (MQ=255)
                            tCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCt                                                                                                                          <  1:4677180/150‑1 (MQ=255)
                             cAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTg                                                                                                                         <  1:49747/150‑1 (MQ=255)
                             cAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTg                                                                                                                         >  2:2609847/1‑150 (MQ=255)
                                gCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCa                                                                                                                      <  1:2140312/150‑1 (MQ=255)
                                    ggCAACTGGTCTCGGTTGCGATCTCCTAACGGCTGGCATTAAACGCTTTTGAGTTCAGCGGCATGGCGGGAAAGATGTATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgct                                                                                                                  <  1:1672283/150‑1 (MQ=255)
                                      cAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgcttt                                                                                                                <  1:5039191/150‑1 (MQ=255)
                                      cAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCGGATTTTGCCCAGCGCGTTGGCATCTAAAAATGGCTGCCCtgcgtt                                                                                                                >  1:1468537/1‑150 (MQ=255)
                                       aaCTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgctttg                                                                                                               <  2:3386677/150‑1 (MQ=255)
                                       aaCTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgctttg                                                                                                               <  2:4028328/150‑1 (MQ=255)
                                        aCTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgctttgc                                                                                                              >  1:760928/1‑150 (MQ=255)
                                                 ggTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTaccaacc                                                                                                     <  1:3129782/150‑1 (MQ=255)
                                                  gTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTaccaacca                                                                                                    <  1:606786/150‑1 (MQ=255)
                                                   tAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCAt                                                                                                   <  1:567975/150‑1 (MQ=255)
                                                   tAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCAt                                                                                                   >  2:4494786/1‑150 (MQ=255)
                                                    aGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCAtt                                                                                                  >  2:1170922/1‑150 (MQ=255)
                                                      cGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCg                                                                                                <  2:1376334/150‑1 (MQ=255)
                                                      cGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCg                                                                                                <  2:2679774/150‑1 (MQ=255)
                                                       gATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCgg                                                                                               >  2:17057/1‑150 (MQ=255)
                                                        aTCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGt                                                                                              <  2:4312866/150‑1 (MQ=255)
                                                        aTCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGt                                                                                              <  1:5099095/150‑1 (MQ=255)
                                                         tCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTc                                                                                             <  2:526287/150‑1 (MQ=255)
                                                         tCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTc                                                                                             >  2:2065614/1‑150 (MQ=255)
                                                            ccTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAAc                                                                                          >  1:894484/1‑150 (MQ=255)
                                                             cTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACg                                                                                         <  1:1006161/150‑1 (MQ=255)
                                                             cTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACg                                                                                         <  1:1577433/150‑1 (MQ=255)
                                                             cTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAAGCTTTTCTTACCAACCATTCGGTCAACg                                                                                         >  1:2773875/1‑150 (MQ=255)
                                                              tAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTTCCATGCTTTGCTTACCAACCATTAGGTCAACGc                                                                                        >  1:3949252/1‑150 (MQ=255)
                                                              tAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCGGGTTTTGCCCAGCGCTTTGGGATCTAAAAAAGGCTGCCATGCGTTGCTTACCAACCATTAGGTCAACGc                                                                                        >  1:2048217/1‑150 (MQ=255)
                                                                aCGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCa                                                                                      <  1:38446/150‑1 (MQ=255)
                                                                aCGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCa                                                                                      >  2:3307618/1‑150 (MQ=255)
                                                                aCGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCa                                                                                      <  1:35604/150‑1 (MQ=255)
                                                                aCGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGAATTTTCCCAGCGCGATGGCAACTAAAAAAGGCAGCCATGCTGTGCATACCAAACAATCGGTAAACGCCa                                                                                      >  2:5117481/1‑150 (MQ=255)
                                                                  ggCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCTGCGCATTTGCATCTAAAAAATGCTGCCAAGCGTTTCTTAACAACCAATAGGTCAACGCCAGc                                                                                    >  1:4078980/1‑150 (MQ=255)
                                                                    cTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGGCAACGCCAGCtt                                                                                  >  2:1980787/1‑150 (MQ=255)
                                                                       gCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCGTTGGCATCTAAAAATGGCTGCCATGCGTTGCTTACCAACCAATCGGTCAACGCCAGCTTCGc                                                                               >  1:3811926/1‑150 (MQ=255)
                                                                       gCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCAGCGCTTTTGCAGCTAAAAAATGCTGCCAAGCTTTGCTTACCAACCAATAGGTCAACCCCAGCTTCGc                                                                               >  1:1896952/1‑150 (MQ=255)
                                                                        cAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATTGCTGCCATGCTTTGCATACCAACCATTCGGTCAACGCCAGCTTCGcc                                                                              >  1:3115857/1‑150 (MQ=255)
                                                                        cAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGcc                                                                              >  2:55580/1‑150 (MQ=255)
                                                                        cAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCGGATTTTGCCCAGCGCTTTGGCCTCAAAAAAATGCGGCCATGCTTTGCGTACCAACCATTCGGTCCACGCCCGCAACGcc                                                                              >  1:1062425/1‑150 (MQ=255)
                                                                         aGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAAGCTTTTCTTACCAACCCTTCGGTCAACGCCAGCTTCGCCa                                                                             >  2:3151003/1‑150 (MQ=255)
                                                                           aaaaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCAtt                                                                           <  2:3902673/150‑1 (MQ=255)
                                                                           aaaaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCAtt                                                                           >  2:5019862/1‑150 (MQ=255)
                                                                           aaaaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCAtt                                                                           <  1:2519683/150‑1 (MQ=255)
                                                                            aaaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGATTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTg                                                                          <  2:677873/150‑1 (MQ=255)
                                                                             aaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTTGCCTCTAAAAATGGCTGCCATGCTTTGCTTACCAACCAATCGGTCAACGCCAGCTTCGCCaattc                                                                         >  2:4585095/1‑146 (MQ=255)
                                                                                 cTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGtt                                                                     <  2:2470782/150‑1 (MQ=255)
                                                                                  ttttCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGttt                                                                    <  2:470498/150‑1 (MQ=255)
                                                                                      cagttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGTTTTTGCCCAGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCAATCGGTCAACGCCAGCTTCGCCATTGCCGTTTCggg                                                                >  1:5136734/1‑149 (MQ=255)
                                                                                      cagttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCAGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCATCGCCCATGCCGTTTCGgt                                                                >  1:1499115/1‑150 (MQ=255)
                                                                                       agttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGgta                                                               <  2:1162292/150‑1 (MQ=255)
                                                                                        gttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCGTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCAATGCCGTgtaggtag                                                              >  2:2800109/1‑150 (MQ=255)
                                                                                         ttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGgtaga                                                             <  1:4273183/150‑1 (MQ=255)
                                                                                          tcagCGGCATGGCGGGAAAGATTGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGgtagag                                                            <  1:4918090/150‑1 (MQ=255)
                                                                                              cGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTTATTTTGCCCTGGGGGTTTGGATATAAAAATGTCTGGCATTGTTTTATTTACAACCAATAGGTAAAAGACAGCTTAGACATTTACGTTTCGgtatagtagt                                                        >  1:1188745/1‑149 (MQ=255)
                                                                                               ggCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGAc                                                       >  2:2792338/1‑150 (MQ=255)
                                                                                               ggCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGAc                                                       <  1:4429775/150‑1 (MQ=255)
                                                                                               ggCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCGTCGCCATTGCCGTTTAGGTAGAGTAGAc                                                       >  2:2944562/1‑150 (MQ=255)
                                                                                                gCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGAcc                                                      <  2:604389/150‑1 (MQ=255)
                                                                                                gCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGAcc                                                      <  2:4043660/150‑1 (MQ=255)
                                                                                                   tGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGAccgcc                                                   >  2:1620300/1‑150 (MQ=255)
                                                                                                      cGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGTTTTTGCCCGGCGCGTTGGCATCTAAAAATTGCTGCCAAGCGTTGCTTACCAACCAATCGGTCAACGCCAGCTTCGCCATTTCCGTTTAGGTAGAGTAGACCGCCGTc                                                >  1:5200756/1‑150 (MQ=255)
                                                                                                       gggAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCAGCGCATTGGCATCGAAAAATGGCAGCCAAGCGTTGCTTACCCACCCATCGGTCAACCCCAGCTACGCCCAAGCCCGGTCGGGAGAGTCGAccccccgca                                               >  2:1877011/1‑146 (MQ=255)
                                                                                                        ggAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTAGGTCAACGCCAGCTTCGCCAATGCCGTTTCGGTAGAGTAGACCGCCGTCAc                                              >  1:4806474/1‑150 (MQ=255)
                                                                                                            aGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTTCCCAGCGCTTTTGCAAAAAAAAAATGCAGCCATGCATTTCTTAACAACCAATAGGTAAAAGCCAGCATCCCCAATGCCCTATCGGTATAGTAGAAAGCCGTAACCGCa                                          >  1:2669293/1‑150 (MQ=255)
                                                                                                            aGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCa                                          >  2:408912/1‑150 (MQ=255)
                                                                                                             gATGGATATCGGCAAAGCGTTTGAAGGAGTCGCGGTTTTTTCCCAGCGCATTGGCAGCTAAAAAATGCTGCCAATCTTTTCTTAACAACCAATAGGGCAACGCCCGCGTCGCCCTTGCCGTTTCGGTAGAGGAGAACGCCGTCACCGCAg                                         >  1:85505/1‑150 (MQ=255)
                                                                                                               tGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAGGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGac                                       <  1:1838300/150‑1 (MQ=255)
                                                                                                                ggATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGaca                                      <  2:4928666/150‑1 (MQ=255)
                                                                                                                  atatCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACAcc                                    <  1:2059185/150‑1 (MQ=255)
                                                                                                                  atatCGGCAAAGCGTTTGAAGGAGTCGCTGAATTTGCCCTGCCCTTTTGCATCAAAAAAAGGCAGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCGTCCCCAATTCCGTTTCGGGAGAGGAGACCGCCGTAAAGCCAGAAAcc                                    >  2:4140918/1‑150 (MQ=255)
                                                                                                                       ggCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGa                               <  1:2157909/150‑1 (MQ=255)
                                                                                                                        gCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGaa                              >  2:834161/1‑150 (MQ=255)
                                                                                                                        gCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCGTTGGGGGGGGAAAATGGGTGCCATGCTTTGCTTACCAACCAATCGGTCAACGCCAGCGTCGCCATTTCCGTTTAGGTAGAGTAGACCGCCGTCACGGCAGACACCCCAGaa                              >  1:5275171/1‑150 (MQ=255)
                                                                                                                          aaaGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCATTTCTTACCAACCATTCGGTCAACGCCAGCTTCGCCAATGCCGTTTCGGTAGAGTAGACCGCCGGCACGGCAGACACCGCAGAAGc                            >  2:3654413/1‑150 (MQ=255)
                                                                                                                               gTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATgg                       <  2:3529218/150‑1 (MQ=255)
                                                                                                                                 ttGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTc                     <  2:1019387/150‑1 (MQ=255)
                                                                                                                                     aGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCt                 <  1:1749799/150‑1 (MQ=255)
                                                                                                                                     aGGAGTCGCTGATTTTGCCCAGCGCTTTTGCAAATAAAAAATGCTGCCAAGCGTTGCTTACCAACCAATCGGTCAACGCCAGCTTAGCCAATGCCGGTTAGGTAGAGTATACCCCCCTCACCGCAGACACCCCACAAGCAAAGGTCGcca                 >  1:4744108/1‑149 (MQ=255)
                                                                                                                                      ggAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCAGCCCTGCATTTCTTACCAACCAATCGGTCAACGCCAGCTTCGCCCTTTCCGTTTAGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAAAGGTCGCCTc                >  2:3060348/1‑150 (MQ=255)
                                                                                                                                           cGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCAtt           <  2:2743057/150‑1 (MQ=255)
                                                                                                                                                 ttttGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTca     <  1:408912/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCGATCGCCTAACGGCTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAACCATTCGGTCAACGCCAGCTTCGCCATTGCCGTTTCGGTAGAGTAGACCGCCGTCACGGCAGACACCGCAGAAGCAATGGTCGCCTCGCATTGAGTCAGCA  >  CP009273/3193180‑3193477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑