Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,205,512 A→C 7.5% L349L (CTA→CTC)  dnaG → DNA primase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,205,5120AC 7.5% 870.3 / 61.4 330L349L (CTA→CTC) dnaGDNA primase
Reads supporting (aligned to +/- strand):  ref base A (145/159);  new base C (18/7);  total (164/166)
Fisher's exact test for biased strand distribution p-value = 2.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

Only 100 of 339 total aligned reads displayed.

GCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTGAT  >  CP009273/3205363‑3205661
                                                                                                                                                     |                                                                                                                                                     
gcgACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTa                                                                                                                                                       >  1:4176187/1‑150 (MQ=255)
gcgACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTa                                                                                                                                                       <  2:1149678/150‑1 (MQ=255)
 cgACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAg                                                                                                                                                      <  1:4772163/150‑1 (MQ=255)
    ccAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTAc                                                                                                                                                   >  1:4564766/1‑150 (MQ=255)
     caacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACg                                                                                                                                                  <  1:1355413/150‑1 (MQ=255)
     caacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACg                                                                                                                                                  >  2:1052251/1‑150 (MQ=255)
     caacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACg                                                                                                                                                  >  2:1821273/1‑150 (MQ=255)
      aacaaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGa                                                                                                                                                 >  1:4610914/1‑150 (MQ=255)
        caaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGaaa                                                                                                                                               <  2:387148/150‑1 (MQ=255)
         aaTGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGaaaa                                                                                                                                              <  1:546814/150‑1 (MQ=255)
           tGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAaaga                                                                                                                                            <  1:650625/150‑1 (MQ=255)
              cATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAgg                                                                                                                                         <  1:1092132/150‑1 (MQ=255)
               aTTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGt                                                                                                                                        <  1:3452669/150‑1 (MQ=255)
               aTTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGt                                                                                                                                        >  1:5114749/1‑150 (MQ=255)
                tttGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTa                                                                                                                                       <  1:3371538/150‑1 (MQ=255)
                 ttGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTaa                                                                                                                                      <  1:1026688/150‑1 (MQ=255)
                    cTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaaga                                                                                                                                   >  1:3763609/1‑150 (MQ=255)
                     tGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaagaa                                                                                                                                  <  2:2993251/150‑1 (MQ=255)
                     tGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaagaa                                                                                                                                  <  2:4745254/150‑1 (MQ=255)
                      gTTATGCCGGCGACCGTGCCGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAaagaag                                                                                                                                 <  2:4298294/150‑1 (MQ=255)
                       ttATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGc                                                                                                                                <  2:2305386/150‑1 (MQ=255)
                         aTGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGt                                                                                                                              >  1:3220221/1‑150 (MQ=255)
                          tGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCCGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGtt                                                                                                                             <  2:80209/150‑1 (MQ=255)
                           gACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGttt                                                                                                                            <  1:2626356/150‑1 (MQ=255)
                           gACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGttt                                                                                                                            >  1:2923166/1‑150 (MQ=255)
                            aCGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTg                                                                                                                           <  1:179169/150‑1 (MQ=255)
                                 gACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                      <  2:1337456/150‑1 (MQ=255)
                                 gACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                      >  2:867495/1‑150 (MQ=255)
                                 gACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                      >  1:4100183/1‑150 (MQ=255)
                                 gACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAgcg                                                                                                                      >  1:1876280/1‑150 (MQ=255)
                                    cGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCgg                                                                                                                   >  2:4149079/1‑150 (MQ=255)
                                       gCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGTGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATg                                                                                                                >  2:5002370/1‑150 (MQ=255)
                                       gCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGTGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATg                                                                                                                >  2:1598931/1‑150 (MQ=255)
                                       gCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATg                                                                                                                <  2:1251508/150‑1 (MQ=255)
                                       gCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATg                                                                                                                <  1:2932607/150‑1 (MQ=255)
                                       gCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATg                                                                                                                <  2:3302766/150‑1 (MQ=255)
                                          ggCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAg                                                                                                             <  2:2839141/150‑1 (MQ=255)
                                          ggCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAg                                                                                                             <  2:1487192/150‑1 (MQ=255)
                                           gCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGc                                                                                                            <  2:3502867/150‑1 (MQ=255)
                                           gCCGCGAGGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGc                                                                                                            <  2:2019492/150‑1 (MQ=255)
                                             cgcgATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAg                                                                                                          <  1:382715/150‑1 (MQ=255)
                                                gATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCg                                                                                                       >  1:4393085/1‑150 (MQ=255)
                                                     cgccTGGCGAGCGCTGTAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCAACGAATTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGAAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGcc                                                                                                  >  2:4386785/1‑150 (MQ=255)
                                                        cTGGCGAGCGCTGGAAACGGCGCTGACATACATGACAGACGGCCGTCAGCTACGCTGTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCAct                                                                                               >  2:4908486/1‑150 (MQ=255)
                                                          ggCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCActct                                                                                             <  2:4789527/150‑1 (MQ=255)
                                                           gCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACtctc                                                                                            >  2:2107069/1‑150 (MQ=255)
                                                             gAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCg                                                                                          <  1:385880/150‑1 (MQ=255)
                                                                cgcTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCAt                                                                                       >  1:4987467/1‑150 (MQ=255)
                                                                      aaaCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGt                                                                                 >  1:4067134/1‑150 (MQ=255)
                                                                       aaCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGtt                                                                                >  1:2443821/1‑150 (MQ=255)
                                                                        aCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGttt                                                                               >  2:1555412/1‑150 (MQ=255)
                                                                        aCGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGttt                                                                               <  1:4888978/150‑1 (MQ=255)
                                                                                gCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCt                                                                       <  1:3914796/150‑1 (MQ=255)
                                                                                  cTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTAAGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGa                                                                     >  2:3691773/1‑150 (MQ=255)
                                                                                   ttACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGAt                                                                    >  2:3149885/1‑150 (MQ=255)
                                                                                   ttACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGAt                                                                    <  2:2751281/150‑1 (MQ=255)
                                                                                   ttACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGAt                                                                    >  2:4474201/1‑150 (MQ=255)
                                                                                    tACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATg                                                                   <  2:2485339/150‑1 (MQ=255)
                                                                                      cATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGcc                                                                 <  2:1961422/150‑1 (MQ=255)
                                                                                      cATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGcc                                                                 >  1:19276/1‑150 (MQ=255)
                                                                                      cATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGcc                                                                 <  2:662702/150‑1 (MQ=255)
                                                                                             gACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGtt                                                          <  1:2887036/150‑1 (MQ=255)
                                                                                              aCGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTg                                                         <  1:3132812/150‑1 (MQ=255)
                                                                                               cGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGa                                                        >  2:1377966/1‑150 (MQ=255)
                                                                                                ggCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGAt                                                       <  2:582166/150‑1 (MQ=255)
                                                                                                ggCCGTCAGCTACGCTTTATGGATTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGACGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGAt                                                       <  2:4043215/150‑1 (MQ=255)
                                                                                                  ccGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCt                                                     <  2:2206944/150‑1 (MQ=255)
                                                                                                  ccGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCt                                                     <  2:106487/150‑1 (MQ=255)
                                                                                                  ccGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCt                                                     <  2:1721455/150‑1 (MQ=255)
                                                                                                    gTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGGGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGa                                                   >  1:4019942/1‑150 (MQ=255)
                                                                                                    gTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGa                                                   >  2:71370/1‑150 (MQ=255)
                                                                                                    gTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGa                                                   <  2:2695522/150‑1 (MQ=255)
                                                                                                      cAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGt                                                 <  2:3661826/150‑1 (MQ=255)
                                                                                                            cGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCt                                           <  1:1970662/150‑1 (MQ=255)
                                                                                                              cTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                         >  1:128416/1‑150 (MQ=255)
                                                                                                              cTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                         >  2:5145003/1‑150 (MQ=255)
                                                                                                              cTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                         <  2:4606195/150‑1 (MQ=255)
                                                                                                              cTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                         >  1:777206/1‑150 (MQ=255)
                                                                                                              cTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGa                                         <  2:3414054/150‑1 (MQ=255)
                                                                                                               tttATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGAc                                        <  1:1847477/150‑1 (MQ=255)
                                                                                                               tttATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGAc                                        <  1:3615168/150‑1 (MQ=255)
                                                                                                                    gTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGgcg                                   <  2:1646111/150‑1 (MQ=255)
                                                                                                                     tttttGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGgcgc                                  <  2:260714/150‑1 (MQ=255)
                                                                                                                           ccTGAAGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGAAAAGAAGCGATTGAAGCGCGGAAGGAGCAGGCGATGCCACTCACCGCAATTCAGTTAAACAGTCTGATGCCGCAAGTAGATCTGAGTACCCCAGACGGGCGCGCACga                            >  2:1040239/1‑149 (MQ=255)
                                                                                                                             tGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGttt                          <  1:1493721/150‑1 (MQ=255)
                                                                                                                                 ggCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGt                      <  2:5151020/150‑1 (MQ=255)
                                                                                                                                    gAAGACCCTGACACGCTCGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACg                   >  2:341774/1‑150 (MQ=255)
                                                                                                                                    gAAGACCCTGACACGCTAGTACGAACAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACg                   <  2:3947533/150‑1 (MQ=255)
                                                                                                                                    gAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACg                   >  1:937230/1‑150 (MQ=255)
                                                                                                                                     aaGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGc                  >  2:1021209/1‑150 (MQ=255)
                                                                                                                                      aGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCt                 >  1:5065226/1‑150 (MQ=255)
                                                                                                                                       gACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTg                >  2:1806155/1‑150 (MQ=255)
                                                                                                                                          ccTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGATTGAGTACGCTGGCa             <  1:1703596/150‑1 (MQ=255)
                                                                                                                                            tGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACt           <  2:2518925/150‑1 (MQ=255)
                                                                                                                                              acacGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTAc         >  1:5173661/1‑150 (MQ=255)
                                                                                                                                                 cGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCAt      >  2:3706986/1‑150 (MQ=255)
                                                                                                                                                  gCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCAtt     <  2:4953877/150‑1 (MQ=255)
                                                                                                                                                  gCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCAtt     <  2:454566/150‑1 (MQ=255)
                                                                                                                                                   cTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTg    <  1:4763684/150‑1 (MQ=255)
                                                                                                                                                     aGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTGat  <  1:664839/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCCTGGCGAGCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCTGATGGCGAAGACCCTGACACGCTAGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTAACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTACCATTGAT  >  CP009273/3205363‑3205661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑