Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,238,283 C→A 100% intergenic (‑183/‑180) uxaC ← / → exuT uronate isomerase/hexuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,238,2830CA99.4% 1080.5 / ‑2.3 331intergenic (‑183/‑180)uxaC/exuTuronate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (182/147);  total (182/149)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 334 total aligned reads displayed.

TATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAA  >  CP009273/3238136‑3238429
                                                                                                                                                   |                                                                                                                                                  
 ctGGTGCGATGCTGACAAAGGTATCACACCACTTTCCAGAGTCCGCAGATATTTGCGTGAGGTAGCTCACTAAACGTAGTTAAAAAAATTACTCTCACAGTGGTAACTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAgg                                                                                                                                                 <  2:2164901/149‑1 (MQ=255)
  tGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGt                                                                                                                                                <  1:1633905/150‑1 (MQ=255)
        gATGCTGATAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTc                                                                                                                                          >  1:1523664/1‑150 (MQ=255)
        gATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTc                                                                                                                                          <  2:1607715/150‑1 (MQ=255)
          tGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTccc                                                                                                                                        <  1:3017774/150‑1 (MQ=255)
          tGCTGACAAAGTTATCAAACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTccc                                                                                                                                        <  1:1168836/150‑1 (MQ=255)
           gCTGATAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCt                                                                                                                                       >  1:2030446/1‑150 (MQ=255)
            cTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTa                                                                                                                                      >  1:3894068/1‑150 (MQ=255)
            cTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGATGTCGTAAGGTAATGTCCCTa                                                                                                                                      >  2:2809595/1‑150 (MQ=255)
            cTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTAATCTCAAAGTGGTAAATCTCTCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTa                                                                                                                                      >  2:3821209/1‑150 (MQ=255)
              gACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTACGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACa                                                                                                                                    >  2:3679532/1‑150 (MQ=255)
                 aaaGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAat                                                                                                                                 >  2:2558946/1‑150 (MQ=255)
                  aaGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAata                                                                                                                                >  2:3512521/1‑150 (MQ=255)
                  aaGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAata                                                                                                                                >  1:4852490/1‑150 (MQ=255)
                   aGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAatat                                                                                                                               <  2:677206/150‑1 (MQ=255)
                    gTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATAtt                                                                                                                              <  2:470939/150‑1 (MQ=255)
                       aTCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTccc                                                                                                                           >  1:4479462/1‑150 (MQ=255)
                       aTCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTccc                                                                                                                           >  2:3033880/1‑150 (MQ=255)
                         cacaCCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTCCTGTCCCTACAAATATTCCcac                                                                                                                         >  2:2413439/1‑150 (MQ=255)
                           cacCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACAt                                                                                                                       >  1:572865/1‑150 (MQ=255)
                             ccAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACAttt                                                                                                                     <  2:322881/150‑1 (MQ=255)
                               actttCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAATTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTtgt                                                                                                                   >  2:2303073/3‑150 (MQ=255)
                               aaTTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTtgt                                                                                                                   >  1:2792442/1‑150 (MQ=255)
                               aaTTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTtgt                                                                                                                   <  2:1840376/150‑1 (MQ=255)
                               aaTTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATCACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTtgt                                                                                                                   <  1:1929676/150‑1 (MQ=255)
                                    ccAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATgg                                                                                                              <  2:5121463/150‑1 (MQ=255)
                                    ccAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATgg                                                                                                              <  2:1758083/150‑1 (MQ=255)
                                      agagTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGct                                                                                                            >  1:3190496/1‑150 (MQ=255)
                                        agTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATAGTCCCACATTTGTGATGGctct                                                                                                          <  1:2517918/150‑1 (MQ=255)
                                         gTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCtctc                                                                                                         <  1:4052961/150‑1 (MQ=255)
                                           ccGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAc                                                                                                       <  2:4081302/150‑1 (MQ=255)
                                            cGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                                                                      >  2:2152134/1‑150 (MQ=255)
                                             gAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt                                                                                                     >  1:3766989/1‑150 (MQ=255)
                                             gAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCt                                                                                                     <  1:4170157/150‑1 (MQ=255)
                                              aaGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtt                                                                                                    >  1:976033/1‑150 (MQ=255)
                                              aaGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtt                                                                                                    <  1:1020143/150‑1 (MQ=255)
                                               aGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCttt                                                                                                   >  1:455426/1‑150 (MQ=255)
                                               aGATAATTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCttt                                                                                                   >  2:4525935/1‑150 (MQ=255)
                                                 atatTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTa                                                                                                 >  1:3500944/1‑150 (MQ=255)
                                                   atTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTaaa                                                                                               >  2:1147479/1‑150 (MQ=255)
                                                     tttCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGt                                                                                             <  1:1662370/150‑1 (MQ=255)
                                                     tttCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGt                                                                                             >  1:2891566/1‑150 (MQ=255)
                                                        cGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGt                                                                                          <  2:2272504/150‑1 (MQ=255)
                                                        cGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCCTTTAAAGTTGt                                                                                          >  2:5015661/1‑150 (MQ=255)
                                                          tGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                                                                        <  1:3621192/150‑1 (MQ=255)
                                                           gAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATg                                                                                       <  2:2094408/150‑1 (MQ=255)
                                                           gAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACATTTTAAAGTTGTATg                                                                                       >  2:1524974/1‑150 (MQ=255)
                                                            aGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGa                                                                                      >  2:4835866/1‑150 (MQ=255)
                                                            aGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGa                                                                                      >  2:133926/1‑150 (MQ=255)
                                                             gTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGAc                                                                                     >  1:4295062/1‑150 (MQ=255)
                                                                aGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAg                                                                                  <  1:4405618/150‑1 (MQ=255)
                                                                 gATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                                                                                 >  2:4245255/1‑150 (MQ=255)
                                                                  aTCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGtt                                                                                <  1:2227939/150‑1 (MQ=255)
                                                                  aTCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGtt                                                                                >  1:3428630/1‑150 (MQ=255)
                                                                    cAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt                                                                              >  2:1232126/1‑150 (MQ=255)
                                                                       tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                                                                           <  2:3173459/150‑1 (MQ=255)
                                                                       tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                                                                           >  1:5120908/1‑150 (MQ=255)
                                                                        aaaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATAGCTCGCACCTTGTACAGGTGTATGAGAAGTTAGCtgt                                                                          >  1:744911/1‑148 (MQ=255)
                                                                         aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTc                                                                         >  1:1413935/1‑150 (MQ=255)
                                                                         aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTc                                                                         <  1:1797072/150‑1 (MQ=255)
                                                                           cGTAGTTAAAAAAATAACTCTCAAAGTGGTAAATCTCTCTGCAGGCCGCGCCAGTACTGGCCATGATGTCGTAAGGTAATGTCCCAACAAATATTCACACAAATGTGATGTCTCTCACCATTTAAAGTAGTATGACAAGTTATCTTTCTg                                                                       >  1:2306339/1‑150 (MQ=255)
                                                                            gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGc                                                                      >  1:3307777/1‑150 (MQ=255)
                                                                             tAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGcc                                                                     >  2:4953025/1‑150 (MQ=255)
                                                                              aGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCg                                                                    >  1:4769511/1‑150 (MQ=255)
                                                                              aGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCAGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCg                                                                    >  2:480178/1‑150 (MQ=255)
                                                                                ttAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTc                                                                  <  1:2165203/150‑1 (MQ=255)
                                                                                      aaaTTACTCTCAAAGTGGTAAATCTCTCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGAAAATTTATCTTTCTGCCGTCGCAAAt                                                            >  1:2343376/1‑150 (MQ=255)
                                                                                      aaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAAt                                                            <  1:2130711/150‑1 (MQ=255)
                                                                                      aaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAAt                                                            >  1:2635417/1‑150 (MQ=255)
                                                                                       aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATc                                                           >  2:5298599/1‑150 (MQ=255)
                                                                                            ctctCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAg                                                      >  1:4694918/1‑150 (MQ=255)
                                                                                             tctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATAGTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGt                                                     <  1:1886793/150‑1 (MQ=255)
                                                                                             tctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGAAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGACGTCGCAAATCATAAGt                                                     >  1:5312307/1‑150 (MQ=255)
                                                                                              ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTc                                                    <  2:2762690/150‑1 (MQ=255)
                                                                                              ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTc                                                    <  2:4962979/150‑1 (MQ=255)
                                                                                              ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTc                                                    <  2:837316/150‑1 (MQ=255)
                                                                                               tcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg                                                   <  1:3044891/150‑1 (MQ=255)
                                                                                                 aaaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGAc                                                 >  2:4393460/1‑150 (MQ=255)
                                                                                                   aGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACgg                                               >  1:1705788/1‑150 (MQ=255)
                                                                                                      ggTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAAt                                            <  1:4978175/150‑1 (MQ=255)
                                                                                                      ggTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAAt                                            <  2:36441/150‑1 (MQ=255)
                                                                                                       gTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATg                                           <  2:3301524/150‑1 (MQ=255)
                                                                                                        tAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGc                                          >  1:3668856/1‑150 (MQ=255)
                                                                                                          aaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCaa                                        >  1:843224/1‑150 (MQ=255)
                                                                                                              tcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTg                                    <  1:1141564/150‑1 (MQ=255)
                                                                                                              tcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTg                                    <  2:4613756/150‑1 (MQ=255)
                                                                                                                   gCAGGCCGCGCCAGGACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATAGTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGat                               <  2:1469840/150‑1 (MQ=255)
                                                                                                                          gcgcCAGTACTGGCCTTGATGTCGAAAGGTAATGACCCTACAAATAATCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATATTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGAttcattca                        >  2:3426931/1‑150 (MQ=255)
                                                                                                                           cgcCAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATtcattcat                       >  1:3214947/1‑150 (MQ=255)
                                                                                                                             ccAGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCAttt                     <  1:3731945/150‑1 (MQ=255)
                                                                                                                               aGTACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGt                   >  2:766770/1‑150 (MQ=255)
                                                                                                                                 tACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                 <  2:2896779/150‑1 (MQ=255)
                                                                                                                                 tACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                 >  1:4103067/1‑150 (MQ=255)
                                                                                                                                 tACTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTa                 <  2:950505/150‑1 (MQ=255)
                                                                                                                                  aCTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAg                >  2:4888252/1‑150 (MQ=255)
                                                                                                                                   cTGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGa               <  2:3059057/150‑1 (MQ=255)
                                                                                                                                    tGGCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGAt              <  2:905636/150‑1 (MQ=255)
                                                                                                                                      gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGa            <  1:4619520/150‑1 (MQ=255)
                                                                                                                                      gCCTTGCTGTCGTAAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGa            <  1:522135/150‑1 (MQ=255)
                                                                                                                                          tGCTGTCGTAAGGTAATGTCCCTACAAATAGTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCg        <  1:1387449/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATGGTGCGATGCTGACAAAGTTATCACACCAATTTCCAGAGTCCGAAGATATTTTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGAATGCAAATTGCCGATTCATTCATTTGTTAGATGAATCGGGTTAA  >  CP009273/3238136‑3238429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑