Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,266,646 C→A 100% G91C (GGT→TGT)  garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,266,6460CA100.0% 1053.8 / ‑6.7 317G91C (GGT→TGT) garLalpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (175/141);  minor base T (0/1);  total (175/142)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 317 total aligned reads displayed.

GAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGT  >  CP009273/3266500‑3266792
                                                                                                                                                  |                                                                                                                                                  
gAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGat                                                                                                                                                 <  2:4961167/150‑1 (MQ=255)
 aaaTAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGata                                                                                                                                                >  1:2104688/1‑150 (MQ=255)
  aataatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGatat                                                                                                                                               <  2:4670946/150‑1 (MQ=255)
   ataatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATc                                                                                                                                              >  1:3591520/1‑150 (MQ=255)
   ataatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATc                                                                                                                                              <  1:4836393/150‑1 (MQ=255)
    taatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATcc                                                                                                                                             <  2:2914876/150‑1 (MQ=255)
     aatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCa                                                                                                                                            <  1:3680481/150‑1 (MQ=255)
     aatCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCa                                                                                                                                            <  2:2249925/150‑1 (MQ=255)
       tCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCaga                                                                                                                                          >  2:2125907/1‑150 (MQ=255)
         cGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCagaag                                                                                                                                        <  1:3161587/150‑1 (MQ=255)
         cGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCagaag                                                                                                                                        <  1:1068364/150‑1 (MQ=255)
            cACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACg                                                                                                                                     >  1:3119053/1‑150 (MQ=255)
             aCGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGc                                                                                                                                    >  1:1184968/1‑150 (MQ=255)
              cGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCt                                                                                                                                   >  2:2827645/1‑150 (MQ=255)
               ggTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCtt                                                                                                                                  >  1:1669437/1‑150 (MQ=255)
               ggTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCtt                                                                                                                                  >  2:140363/1‑150 (MQ=255)
                gTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTta                                                                                                                                 >  2:376879/1‑150 (MQ=255)
                 tGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaa                                                                                                                                <  1:2881664/150‑1 (MQ=255)
                 tGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaa                                                                                                                                >  1:1511854/1‑150 (MQ=255)
                 tGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTtaa                                                                                                                                >  1:2164116/1‑150 (MQ=255)
                     aaaCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTaataat                                                                                                                            >  2:1951742/1‑150 (MQ=255)
                     aaaCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTaataat                                                                                                                            <  2:1525468/150‑1 (MQ=255)
                      aaCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAAtt                                                                                                                           >  1:1858328/1‑150 (MQ=255)
                      aaCATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAAtt                                                                                                                           >  2:590944/1‑150 (MQ=255)
                        cATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAc                                                                                                                         >  1:820624/1‑150 (MQ=255)
                        cATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAc                                                                                                                         <  2:2698518/150‑1 (MQ=255)
                         atatTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTAcc                                                                                                                        >  1:4686794/1‑150 (MQ=255)
                           atTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGACTCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCgg                                                                                                                      >  2:112588/1‑150 (MQ=255)
                           atTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCgg                                                                                                                      >  2:1287927/1‑150 (MQ=255)
                              ggCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTc                                                                                                                   <  2:4986697/150‑1 (MQ=255)
                               gcgcGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCg                                                                                                                  <  2:698406/150‑1 (MQ=255)
                                cgcgGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGt                                                                                                                 >  2:1777797/1‑150 (MQ=255)
                                 gcgGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGtt                                                                                                                >  1:4976891/1‑150 (MQ=255)
                                     tGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTc                                                                                                            <  2:4656997/150‑1 (MQ=255)
                                      gagaAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCg                                                                                                           >  2:355849/1‑150 (MQ=255)
                                          aaCGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCAc                                                                                                       <  2:3405919/150‑1 (MQ=255)
                                            cGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTc                                                                                                     >  1:600261/1‑150 (MQ=255)
                                                gaCGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCac                                                                                                 >  2:2066080/1‑150 (MQ=255)
                                                      gcgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTgg                                                                                           >  2:3294062/1‑150 (MQ=255)
                                                       cgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgc                                                                                          >  1:3503148/1‑150 (MQ=255)
                                                       cgAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgc                                                                                          <  1:3182867/150‑1 (MQ=255)
                                                          aTGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGgcgcg                                                                                       >  2:3243730/1‑150 (MQ=255)
                                                                  cGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgct                                                                               >  2:1501180/1‑150 (MQ=255)
                                                                  cGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCgcgctggcgct                                                                               >  2:259312/1‑150 (MQ=255)
                                                                     tGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGcc                                                                            <  2:2408593/150‑1 (MQ=255)
                                                                       ggTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCATAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCtt                                                                          >  1:4083717/1‑150 (MQ=255)
                                                                         tAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTc                                                                        <  2:3935703/150‑1 (MQ=255)
                                                                         tAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTc                                                                        <  1:2968742/150‑1 (MQ=255)
                                                                            cGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAg                                                                     <  2:3060296/150‑1 (MQ=255)
                                                                               gTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGcc                                                                  <  1:2741015/150‑1 (MQ=255)
                                                                                  gATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCAtt                                                               <  2:448337/150‑1 (MQ=255)
                                                                                   aTGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTa                                                              <  1:1241884/150‑1 (MQ=255)
                                                                                    tGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTaa                                                             >  2:349975/1‑150 (MQ=255)
                                                                                     gCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAAc                                                            <  1:4920242/150‑1 (MQ=255)
                                                                                     gCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAAc                                                            >  1:2381443/1‑150 (MQ=255)
                                                                                      ccACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACt                                                           <  1:3463989/150‑1 (MQ=255)
                                                                                      ccACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACt                                                           <  2:4185447/150‑1 (MQ=255)
                                                                                          cGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCgg                                                       <  2:727001/150‑1 (MQ=255)
                                                                                              aGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATa                                                   <  1:423973/150‑1 (MQ=255)
                                                                                                 tctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAAc                                                >  2:4275822/1‑150 (MQ=255)
                                                                                                 tctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAAc                                                >  1:1002530/1‑150 (MQ=255)
                                                                                                 tctGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAAc                                                <  1:2371839/150‑1 (MQ=255)
                                                                                                     cTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTgg                                            >  1:5269976/1‑150 (MQ=255)
                                                                                                     cTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTgg                                            <  1:2040041/150‑1 (MQ=255)
                                                                                                     cTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAAATACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGACTAAACGTgg                                            >  2:855000/1‑150 (MQ=255)
                                                                                                        ccTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgaga                                         >  2:1564386/1‑150 (MQ=255)
                                                                                                        ccTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgaga                                         >  1:931532/1‑150 (MQ=255)
                                                                                                        ccTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGgaga                                         >  1:3807944/1‑150 (MQ=255)
                                                                                                         ctctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGat                                        <  1:246182/150‑1 (MQ=255)
                                                                                                         ctctTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGat                                        <  1:2498206/150‑1 (MQ=255)
                                                                                                             tttGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCg                                    >  1:1841731/1‑150 (MQ=255)
                                                                                                             tttGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCg                                    >  1:2408818/1‑150 (MQ=255)
                                                                                                                 tttCTACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTg                                >  1:5179153/1‑150 (MQ=255)
                                                                                                                     tACAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGgcgc                            >  1:1730345/1‑150 (MQ=255)
                                                                                                                      aCAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCa                           >  1:3411019/1‑150 (MQ=255)
                                                                                                                       cAAAAGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCAt                          >  1:3898627/1‑150 (MQ=255)
                                                                                                                        aaaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGGGGAGATATCGGTTGGCGCATg                         >  1:5036074/1‑150 (MQ=255)
                                                                                                                         aaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGTGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                        <  2:5236455/150‑1 (MQ=255)
                                                                                                                         aaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                        >  2:5054492/1‑150 (MQ=255)
                                                                                                                         aaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                        <  1:2827645/150‑1 (MQ=255)
                                                                                                                         aaaGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGt                        <  2:1663842/150‑1 (MQ=255)
                                                                                                                          aaGGAATAAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGtt                       <  1:4620988/150‑1 (MQ=255)
                                                                                                                           aGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTc                      >  2:5079927/1‑150 (MQ=255)
                                                                                                                           aGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTc                      <  1:355849/150‑1 (MQ=255)
                                                                                                                           aGGAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGAGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTc                      <  1:3773467/150‑1 (MQ=255)
                                                                                                                            ggAATCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCg                     >  1:741479/1‑150 (MQ=255)
                                                                                                                             gAATCAGGAAGTTATAGAAACAGATATCCAGAAGTCGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAATGCCTTTATCTGCGGAATAAACGTGGATATATCGTTTGGCGCATGTTCGc                    <  1:4527768/150‑1 (MQ=255)
                                                                                                                                tCAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCAt                 >  1:2920501/1‑150 (MQ=255)
                                                                                                                                 cAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGACTAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATc                >  1:2786129/1‑150 (MQ=255)
                                                                                                                                 cAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATc                <  1:1951742/150‑1 (MQ=255)
                                                                                                                                 cAGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATc                >  2:2003767/1‑150 (MQ=255)
                                                                                                                                  aGGAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATcc               >  2:1855158/1‑150 (MQ=255)
                                                                                                                                    gAAGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAg             >  1:2688696/1‑150 (MQ=255)
                                                                                                                                      aGTTATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGAGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCa           >  1:3204278/1‑150 (MQ=255)
                                                                                                                                        ttATAGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcc         >  2:3693743/1‑150 (MQ=255)
                                                                                                                                         tataGAAACAGATATCCAGAAGACGCTTAATAATTACCTGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcca        >  2:3152562/1‑150 (MQ=255)
                                                                                                                                         tataGAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCTCTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAcca        <  2:2774997/150‑1 (MQ=255)
                                                                                                                                             gAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccagcca    >  1:136989/1‑150 (MQ=255)
                                                                                                                                             gAAACAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCAccagcca    >  1:4220881/1‑150 (MQ=255)
                                                                                                                                               aaCAGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGt  >  2:1101411/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAAATAATCCGCCACGGTGCCAAACATATTGGCGCGGTGAGAAACGGAGACGCCGCGAATGCCTTCCGGTGGGTAACGGGTTGATGCCACCGCCAGCTCTGCTTCCTCTTTTGTTTCTACAAAAGGAATCAGGAAGTTATAGAAACCGATATCCAGAAGACGCTTAATAATTACCGGCTCGTTGGTCGGCACTCGCACTACTGGCGCGCTGGCGCTGCCTTTCAAGGCCATTAACTGCGGAATAAACGTGGAGATATCGTTTGGCGCATGTTCGCCATCCAGCACCAGCCAGT  >  CP009273/3266500‑3266792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑