Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,275,210 A→G 100% intergenic (+226/‑125) agaA → / → agaS pseudogene, N‑acetylgalactosamine‑6‑phosphate deacetylase fragment; putative N‑acetylgalactosamine‑6‑phosphate deacetylase/tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,275,2100AG99.9% 1121.5 / ‑7.1 303intergenic (+226/‑125)agaA/agaSpseudogene, N‑acetylgalactosamine‑6‑phosphate deacetylase fragment; putative N‑acetylgalactosamine‑6‑phosphate deacetylase/tagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/3);  major base G (128/161);  minor base T (0/11);  total (128/175)
Fisher's exact test for biased strand distribution p-value = 3.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 303 total aligned reads displayed.

CGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTG  >  CP009273/3275063‑3275356
                                                                                                                                                    |                                                                                                                                                  
  gATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGa                                                                                                                                                >  1:297328/1‑150 (MQ=255)
   aTGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGaa                                                                                                                                               <  1:3426548/150‑1 (MQ=255)
    tGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAAc                                                                                                                                              <  1:314881/150‑1 (MQ=255)
     gCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACt                                                                                                                                             <  1:271865/150‑1 (MQ=255)
     gCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACt                                                                                                                                             <  1:557533/150‑1 (MQ=255)
          gCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAg                                                                                                                                        <  1:3668056/150‑1 (MQ=255)
            tAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGtt                                                                                                                                      <  2:558656/150‑1 (MQ=255)
             aaCGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGttt                                                                                                                                     >  1:3322730/1‑150 (MQ=255)
               cgcgTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGtttct                                                                                                                                   >  1:4018063/1‑150 (MQ=255)
                gcgTCTTATCCGACCTACAGTTGGTGACCGCAAGGCATGATAAAGCGTTTGCTCCGAATCCAGCAATCCCTTTTGATTCATTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGtttctt                                                                                                                                  <  1:323609/150‑1 (MQ=255)
                 cgTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGtttcttt                                                                                                                                 <  1:569526/150‑1 (MQ=255)
                  gTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTACGTTGAACTTTCAGtttctttt                                                                                                                                >  2:3130636/1‑150 (MQ=255)
                   tCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGATTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGtttcttttc                                                                                                                               <  2:1956867/150‑1 (MQ=255)
                    cTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGtttcttttct                                                                                                                              >  2:3002420/1‑150 (MQ=255)
                       aTCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCtata                                                                                                                           <  2:717264/150‑1 (MQ=255)
                           gACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGAtt                                                                                                                       <  1:2161433/150‑1 (MQ=255)
                            aCCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGAttt                                                                                                                      <  2:1558462/150‑1 (MQ=255)
                             ccTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGAtttt                                                                                                                     <  1:3118421/150‑1 (MQ=255)
                               tACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTaa                                                                                                                   >  1:3603120/1‑150 (MQ=255)
                                 cAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATc                                                                                                                 <  2:3831814/150‑1 (MQ=255)
                                    ttGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAAc                                                                                                              <  2:3226745/150‑1 (MQ=255)
                                      ggTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGa                                                                                                            >  2:4209894/1‑150 (MQ=255)
                                      ggTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGa                                                                                                            >  2:5231388/1‑150 (MQ=255)
                                            cGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACa                                                                                                      <  2:805136/150‑1 (MQ=255)
                                             gCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACAt                                                                                                     <  1:953623/150‑1 (MQ=255)
                                               aaGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCa                                                                                                   >  2:4674754/1‑150 (MQ=255)
                                               aaGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTATTTTCTATAGATTTTAATCAACGAAAGACATCa                                                                                                   <  1:670681/150‑1 (MQ=255)
                                                  gCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCa                                                                                                >  1:4058403/1‑150 (MQ=255)
                                                   ccGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCaa                                                                                               <  2:186967/150‑1 (MQ=255)
                                                    cGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAg                                                                                              <  2:2724511/150‑1 (MQ=255)
                                                      gATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGtg                                                                                            <  2:1906015/150‑1 (MQ=255)
                                                          aaGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGtgaaat                                                                                        <  1:1709773/150‑1 (MQ=255)
                                                          aaGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGtgaaat                                                                                        >  1:3963283/1‑150 (MQ=255)
                                                          aaGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCATTGAATTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGtgaaat                                                                                        <  2:2337970/150‑1 (MQ=255)
                                                           aGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGtgaaatg                                                                                       <  1:1684424/150‑1 (MQ=255)
                                                            gCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGtgaaatga                                                                                      <  2:119141/150‑1 (MQ=255)
                                                                ttGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACg                                                                                  <  2:2341770/150‑1 (MQ=255)
                                                                ttGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACg                                                                                  <  1:4791644/150‑1 (MQ=255)
                                                                  gcgcCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGaa                                                                                <  1:4938751/150‑1 (MQ=255)
                                                                  gcgcCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGaa                                                                                <  2:2422260/150‑1 (MQ=255)
                                                                    gccgcATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAg                                                                              <  1:3916924/150‑1 (MQ=255)
                                                                     ccgcATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATAACCAAGTGAAATGAAACGAAAgg                                                                             >  2:4451755/1‑150 (MQ=255)
                                                                        cATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCaa                                                                          >  1:2819406/1‑150 (MQ=255)
                                                                         aTCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCaa                                                                          >  1:1270467/1‑150 (MQ=255)
                                                                         aTCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAg                                                                         >  2:1017503/1‑150 (MQ=255)
                                                                               cAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAg                                                                   >  2:454252/1‑150 (MQ=255)
                                                                               cAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAg                                                                   >  1:5089945/1‑150 (MQ=255)
                                                                                aaTCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGc                                                                  >  2:5141448/1‑150 (MQ=255)
                                                                                 aTCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCg                                                                 >  2:4411368/1‑150 (MQ=255)
                                                                                   cccTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGAc                                                               >  2:1642237/1‑150 (MQ=255)
                                                                                   cccTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAAAGAAAGTCAAGTTAAATCGAc                                                               >  1:1932517/1‑150 (MQ=255)
                                                                                    ccTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACa                                                              >  1:3490912/1‑150 (MQ=255)
                                                                                    ccTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACa                                                              >  2:2781486/1‑150 (MQ=255)
                                                                                    ccTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAAAGAAAGACATCACCAAGTGAAATGAAAAGAAAGGCAAGTGAAAGCGACa                                                              >  1:4674285/1‑150 (MQ=255)
                                                                                       tttGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACg                                                           <  2:316712/150‑1 (MQ=255)
                                                                                       tttGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACg                                                           >  2:4960295/1‑150 (MQ=255)
                                                                                        ttGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGc                                                          >  1:4188297/1‑150 (MQ=255)
                                                                                        ttGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGc                                                          >  1:456163/1‑150 (MQ=255)
                                                                                          gattCCTTTATCTTTTCTTTAAACTATCAAAAATTTCTTTTTATTTA‑TTTTTTCGCCGTTGAACTTTCTTTTTCTTTTCTATATATTTTAATAAACGAATTACATCACCAAGTTAAATGAAACTAAAGGCAAGTGAAAGCGACAACGccc                                                        <  2:1360620/148‑1 (MQ=255)
                                                                                           cTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCg                                                       <  2:3074568/150‑1 (MQ=255)
                                                                                               cTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGt                                                   >  1:2209558/1‑150 (MQ=255)
                                                                                                      ttttcttaCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCTTTTAACTTTCAGTTTCTTTTCTATATATTTTAATCAACGAAAGACATCACCAAGTGAAATTAAACGAAAGGCAAGTTAAAGCGACAACGCCCGACGTCAAGTtc                                            <  1:633925/150‑1 (MQ=255)
                                                                                                        ttctttcAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTtcat                                          >  1:2346795/1‑150 (MQ=255)
                                                                                                         tcttacAACTATCAAAAATTTCTTTTTATTTC‑TTTTTTCTCCTTTGAACTTTCTTTTTCTTTTCTATATATTTTAATCAACTAAATACAACACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTtcatc                                         <  1:774375/150‑1 (MQ=255)
                                                                                                          ctttcAACGAACACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCACGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTtcatca                                        <  2:3762539/150‑1 (MQ=255)
                                                                                                            ttcAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGa                                      >  1:5131743/1‑150 (MQ=255)
                                                                                                            tacAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGa                                      <  2:3354182/148‑1 (MQ=255)
                                                                                                              cAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGTCt                                    >  2:4781158/1‑150 (MQ=255)
                                                                                                               aaCGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTa                                   >  2:4341528/1‑150 (MQ=255)
                                                                                                                 cGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATTAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAg                                 >  2:4403221/1‑150 (MQ=255)
                                                                                                                     cacaAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCATTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGAtt                             <  2:5019133/150‑1 (MQ=255)
                                                                                                                      acaAATTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTg                            >  1:4249725/1‑150 (MQ=255)
                                                                                                                        aaaTTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAg                          >  2:3555836/1‑150 (MQ=255)
                                                                                                                         aaTTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGt                         >  2:724151/1‑150 (MQ=255)
                                                                                                                          aTTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGtt                        >  2:3436267/1‑150 (MQ=255)
                                                                                                                          aTTTCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGtt                        >  1:3822337/1‑150 (MQ=255)
                                                                                                                           tttCTTTTTTTTTC‑TTTTTACGCCGATTACCTTACATTTTCTTTTATATCTATTTAAAACAACGAAACAAATCACCAATTGAAATGAAACGAAAGGCAAGTCAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTa                       <  1:1946649/150‑1 (MQ=255)
                                                                                                                            ttCTTTTTTTTTC‑TTTTTTCTCCGTTTAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCACGTTAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTAt                      <  1:3834907/150‑1 (MQ=255)
                                                                                                                            ttCGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTAt                      <  2:2618573/150‑1 (MQ=255)
                                                                                                                              cGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGc                    >  2:2085231/1‑150 (MQ=255)
                                                                                                                              cGTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGc                    >  1:2630058/1‑150 (MQ=255)
                                                                                                                               gTTTTATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAATGATTGAGTTATGcc                   >  1:5145517/1‑150 (MQ=255)
                                                                                                                                 tttATTTC‑TTTTTTCTCCGTTTAACTTTCATTTTCTTTTCTATAGATTTAAATAAACTAAAGACATCACCAAGTTAAATTAAACTAAAGGCATGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAg                 <  1:519547/150‑1 (MQ=255)
                                                                                                                                 tttATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCACGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAg                 <  2:5270370/150‑1 (MQ=255)
                                                                                                                                 tttATTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAg                 <  2:1753284/150‑1 (MQ=255)
                                                                                                                                   tATTTC‑TTTTTTCTCCGCTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGaa               >  1:1098312/1‑150 (MQ=255)
                                                                                                                                    aTTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATATATTTTAATCAACTAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGaaa              <  1:5231661/150‑1 (MQ=255)
                                                                                                                                    aTTTC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGaaa              >  2:1100270/1‑150 (MQ=255)
                                                                                                                                      ttC‑TTTTTTCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATTAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAAt            <  1:1796138/150‑1 (MQ=255)
                                                                                                                                       tC‑TTTTTTCTCCTTTTAAATTTCTTTTTCTTTTATATATATTTTAAACAACTAAAGACAACACCAAGTTAAATGAAACGAAAGGCAAGTTAACGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAAtt           <  1:1472024/150‑1 (MQ=255)
                                                                                                                                          ttttttCTCCGTTTAACTTTCAGTTTCTTTTCTAGAGATTTTAATCAACGAAAGACATCACCAAGTTAAATTAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTac         <  2:4040962/150‑1 (MQ=255)
                                                                                                                                          ttttttCTCCGTTGAACATTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTac         >  1:3712963/1‑150 (MQ=255)
                                                                                                                                           tttttCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTaca        <  2:1727792/150‑1 (MQ=255)
                                                                                                                                           tttttCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTaca        >  2:485317/1‑150 (MQ=255)
                                                                                                                                             tttCCCCTTTTAACTTTCAGTTTATTTTCTATAGATTTTAAACAACTAAAGACATCACCAAGTTAAATTAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACAcc      <  1:2435329/150‑1 (MQ=255)
                                                                                                                                              ttCTCCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACAccc     >  1:5310610/1‑150 (MQ=255)
                                                                                                                                               tctcCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACAcccc    >  1:447287/1‑150 (MQ=255)
                                                                                                                                                ctcCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCct   >  2:1253519/1‑150 (MQ=255)
                                                                                                                                                ctcCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCct   >  1:3541012/1‑150 (MQ=255)
                                                                                                                                                 tcCGTTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCctg  <  2:3963283/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGATGCGACGCTAACGCGTCTTATCCGACCTACAGTTGGTGACCGCAAGGCCGGATAAAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTC‑TTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTG  >  CP009273/3275063‑3275356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑