Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,299,240 A→C 100% intergenic (‑64/+90) mtr ← / ← deaD tryptophan transporter of high affinity/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,299,2400AC99.6% 996.2 / ‑5.4 252intergenic (‑64/+90)mtr/deaDtryptophan transporter of high affinity/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (134/115);  total (136/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 252 total aligned reads displayed.

CCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCG  >  CP009273/3299093‑3299384
                                                                                                                                                   |                                                                                                                                                
cccTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCg                                                                                                                                                >  1:984555/1‑150 (MQ=255)
  cTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAg                                                                                                                                              <  2:4775456/150‑1 (MQ=255)
   tGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGt                                                                                                                                             >  1:569756/1‑150 (MQ=255)
   tGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGATTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGt                                                                                                                                             <  1:1581303/150‑1 (MQ=255)
         aataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACaaaa                                                                                                                                       >  2:2126/1‑150 (MQ=255)
         aataatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACaaaa                                                                                                                                       >  1:2980340/1‑150 (MQ=255)
           taatGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGa                                                                                                                                     <  2:1971448/150‑1 (MQ=255)
             atGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACa                                                                                                                                   <  1:3296652/150‑1 (MQ=255)
             atGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACa                                                                                                                                   >  1:2834451/1‑150 (MQ=255)
             atGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACa                                                                                                                                   >  1:3447201/1‑150 (MQ=255)
              tGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAg                                                                                                                                  <  2:86142/150‑1 (MQ=255)
               ggTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGa                                                                                                                                 <  2:2102820/150‑1 (MQ=255)
                    cgccgATAATCACCACGCCGCCAAGCATCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGaaaaaa                                                                                                                            <  1:4637260/150‑1 (MQ=255)
                     gccgATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGaaaaaaa                                                                                                                           >  1:5030669/1‑150 (MQ=255)
                        gATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGcc                                                                                                                        >  2:4456788/1‑150 (MQ=255)
                        gATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGcc                                                                                                                        >  2:1122640/1‑150 (MQ=255)
                          tAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGcccc                                                                                                                      <  1:272109/150‑1 (MQ=255)
                           aaTCACCACGCGGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCTTGTGGGGTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCAGTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCg                                                                                                                     <  1:4306919/150‑1 (MQ=255)
                           aaTCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGTTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCAATGCACTGTACCAGAACCCGAGTACAAAATACAGAAAAAAAGACCCg                                                                                                                     >  2:831159/1‑150 (MQ=255)
                           aaTCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCg                                                                                                                     <  2:4880793/150‑1 (MQ=255)
                              cactacGCCGCCAAGCCGCGACGGTGACTTTTGGGTGGTGGTTTGTGTTGCCATGTGGGCTTCTCTCCTGTGAAAAATAGTGCGACTGCGTTGTTTTGCTTTGCACTGTACCAGTACCCGAGTAAAAAAGACAGAAAAAAAGCCCCGATg                                                                                                                  <  1:2633959/150‑1 (MQ=255)
                               accacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATgg                                                                                                                 >  2:5297910/1‑150 (MQ=255)
                                 cacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTTGTGTTGCCATGAGGGATTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACATAAAAAAAGCCCCGATGGTa                                                                                                               <  1:2251105/150‑1 (MQ=255)
                                 cacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTa                                                                                                               >  2:5253003/1‑150 (MQ=255)
                                 cacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTa                                                                                                               >  2:70696/1‑150 (MQ=255)
                                    gccgccAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTaaaa                                                                                                            <  2:360048/150‑1 (MQ=255)
                                       gccAAGCTGCGAGGGTGTGGTTTGGGTGGTGTTTTGTGTTGCCTTGTGGGCTTCTCTCCTTTTAAAATTTGTGCTACTGCGTTGTTTTGATTTGCACTTTACCTGTACCCGAGTAAAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATc                                                                                                         <  1:2403367/150‑1 (MQ=255)
                                          aaGCAGCGCCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCATTGAAAAATAGTGCTACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAACGCCAGAAAAAAAGCCCCGATGGTAAAAATCggg                                                                                                      <  2:3175904/150‑1 (MQ=255)
                                           agctgcGCCGGTGACGTTTGGGGGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATGGCACTGTACCAGTACCCGAGTACAAAATCCAGAAAAAAAGCCCCGATGGTAAAAATCgggg                                                                                                     <  2:1878798/150‑1 (MQ=255)
                                                 gACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtat                                                                                               >  2:3035313/1‑150 (MQ=255)
                                                  aCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtata                                                                                              <  2:4268331/150‑1 (MQ=255)
                                                   gggTGAGGTTGGGGTGGTGTTTTGTTTTGCATTGTGGGATTCTCTCCAGTTAAAAATTGTGGGACTGTTTTTTTATGCATTGAAGTGTACCATTCCCCTTTTAAAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                             <  1:52942/149‑1 (MQ=255)
                                                   cGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatat                                                                                             >  1:4406998/1‑150 (MQ=255)
                                                    ggTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatata                                                                                            >  1:4244219/1‑150 (MQ=255)
                                                    ggTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatata                                                                                            >  1:4141937/1‑150 (MQ=255)
                                                     gTGACTTTTGGGTGTTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTTAAAAATAGTGCTACTGCGTTTTTATGCATTGCAATGAACACTTACCCTAGTACAAAACACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatatag                                                                                           <  1:5206459/150‑2 (MQ=255)
                                                        aCGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATAtattat                                                                                        >  2:2454883/1‑150 (MQ=255)
                                                         cGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATCTattatt                                                                                       <  2:3408947/150‑1 (MQ=255)
                                                          gTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAttt                                                                                      <  2:703319/150‑1 (MQ=255)
                                                             tGGGTGGTGGTTTGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCATTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTAc                                                                                   <  2:5150065/150‑1 (MQ=255)
                                                               ggtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAg                                                                                 <  2:2590683/150‑1 (MQ=255)
                                                                gtggtggtTTGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTTTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                <  1:2860099/150‑1 (MQ=255)
                                                                 tggtggtTTGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGAt                                                                               <  2:3336268/150‑1 (MQ=255)
                                                                 tggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGAt                                                                               <  1:337284/150‑1 (MQ=255)
                                                                    tggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgt                                                                            >  2:2743605/1‑150 (MQ=255)
                                                                    tggtTAGTGTTGCCATGAGGGCTACTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATACCACAGTACCAGTACCCGAGTACAAAAGACAGAACAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgt                                                                            >  2:769152/1‑150 (MQ=255)
                                                                     ggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTCTCTATTATTTTACAGATtgtg                                                                           >  2:907748/1‑150 (MQ=255)
                                                                     ggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                           >  2:4224827/1‑150 (MQ=255)
                                                                     ggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                           >  1:2081036/1‑150 (MQ=255)
                                                                      gtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTgtgt                                                                          >  1:1610918/1‑150 (MQ=255)
                                                                           tgtTGCCATGAGGGCTTCGCTCCAGTGAAAAATAGTTCTACTGCTTTGTTATGCATTGCACTGTACACGTCCCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCt                                                                     <  1:1161062/150‑1 (MQ=255)
                                                                             ttGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGt                                                                   <  2:638458/150‑1 (MQ=255)
                                                                               gCCATGAGGGCTTCTCTCCAGTTAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTAAAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTc                                                                 <  1:20183/150‑1 (MQ=255)
                                                                                 cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAg                                                               >  1:3994886/1‑150 (MQ=255)
                                                                                  aTGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                              >  2:1414471/1‑150 (MQ=255)
                                                                                    gAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCga                                                            >  2:3794262/1‑150 (MQ=255)
                                                                                     aGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACATATTGTGTTCGCTGTTCAGCgat                                                           >  2:2388383/1‑150 (MQ=255)
                                                                                     aGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgat                                                           >  2:1360737/1‑150 (MQ=255)
                                                                                     aGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgat                                                           <  2:2722355/150‑1 (MQ=255)
                                                                                      gggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatg                                                          <  2:1663549/150‑1 (MQ=255)
                                                                                        gCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatgat                                                        <  2:4695680/150‑1 (MQ=255)
                                                                                        gCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatgat                                                        >  1:724412/1‑150 (MQ=255)
                                                                                         cTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAtt                                                       <  2:119035/150‑1 (MQ=255)
                                                                                          ttCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTa                                                      >  1:1431009/1‑150 (MQ=255)
                                                                                             tctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGc                                                   >  1:4808867/1‑150 (MQ=255)
                                                                                              ctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                  >  1:3545194/1‑150 (MQ=255)
                                                                                                ccAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                >  1:4196081/1‑150 (MQ=255)
                                                                                                ccAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                >  1:4760691/1‑150 (MQ=255)
                                                                                                 cAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATca                                               <  1:401799/150‑1 (MQ=255)
                                                                                                  aGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcac                                              >  2:4868067/1‑150 (MQ=255)
                                                                                                   gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                             >  2:1832881/1‑150 (MQ=255)
                                                                                                   gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacc                                             >  1:3062206/1‑150 (MQ=255)
                                                                                                    tGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcacca                                            >  2:1905447/1‑150 (MQ=255)
                                                                                                      aaaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCaccacc                                          >  1:2553989/1‑150 (MQ=255)
                                                                                                       aaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCg                                         >  1:2218710/1‑150 (MQ=255)
                                                                                                        aaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTCTATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGa                                        >  2:2884846/1‑150 (MQ=255)
                                                                                                         aaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGaa                                       >  2:104816/1‑150 (MQ=255)
                                                                                                           tAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAac                                     <  1:5237584/150‑1 (MQ=255)
                                                                                                           tAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAac                                     >  2:2671671/1‑150 (MQ=255)
                                                                                                            aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                    <  2:2765968/150‑1 (MQ=255)
                                                                                                            aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                    >  1:1047179/1‑150 (MQ=255)
                                                                                                              tGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgac                                  <  1:890425/150‑1 (MQ=255)
                                                                                                                   cTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAAcgacgacga                             >  2:923192/1‑150 (MQ=255)
                                                                                                                      cGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCg                          >  2:4051399/1‑150 (MQ=255)
                                                                                                                              tGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTACAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatc                  >  2:4629061/1‑150 (MQ=255)
                                                                                                                              tGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTCGAatc                  >  1:4271482/1‑150 (MQ=255)
                                                                                                                                cATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcat                <  2:2049250/150‑1 (MQ=255)
                                                                                                                                cATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcat                >  1:1669206/1‑150 (MQ=255)
                                                                                                                                 aTTGCACTGTACCAGTACCCGAGTACAAAAGACATAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc               <  2:202685/150‑1 (MQ=255)
                                                                                                                                 aTTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc               >  1:3685734/1‑150 (MQ=255)
                                                                                                                                  ttGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAAtcatca              <  2:3952431/150‑1 (MQ=255)
                                                                                                                                   tGCACTGTACCAGTACCCGAGTACAACAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCac             <  2:533966/150‑1 (MQ=255)
                                                                                                                                   tGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCac             <  2:3447201/150‑1 (MQ=255)
                                                                                                                                    gCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacg            <  2:3689618/150‑1 (MQ=255)
                                                                                                                                     cACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCTCCGAAACGACGACGACCGGTAGAATCATCacga           >  1:1500330/1‑150 (MQ=255)
                                                                                                                                      aCTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacgac          <  2:5166595/150‑1 (MQ=255)
                                                                                                                                            ccAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAg    <  2:2511348/150‑1 (MQ=255)
                                                                                                                                             cAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgc   >  1:1866953/1‑150 (MQ=255)
                                                                                                                                             cAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgc   >  2:32227/1‑150 (MQ=255)
                                                                                                                                              aGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgcg  >  2:398933/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCG  >  CP009273/3299093‑3299384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑