Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,312,055 C→A 100% G20G (GGC→GGA)  argG → argininosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,312,0550CA99.0% 933.5 / ‑1.1 286G20G (GGC→GGA) argGargininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (154/129);  total (156/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 286 total aligned reads displayed.

TAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGGAGAACGCACG  >  CP009273/3311908‑3312201
                                                                                                                                                   |                                                                                                                                                  
tAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAgg                                                                                                                                                  <  1:159738/150‑1 (MQ=255)
  aaTGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTc                                                                                                                                                <  2:4528821/150‑1 (MQ=255)
  aaTGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTc                                                                                                                                                >  1:4882404/1‑150 (MQ=255)
    tGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTg                                                                                                                                              >  1:4042123/1‑150 (MQ=255)
    tGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTg                                                                                                                                              <  1:2203382/150‑1 (MQ=255)
    tGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTg                                                                                                                                              >  1:1968928/1‑150 (MQ=255)
     gAAAACTCATTTATTTTGCATAAAAAATCAGTGAGAGCGGAAATCCAGGCTCATCAGCAGTTAATTAAGCAGGTTGTTATTTTATGACTACGATTCTCAATCATCTCCCGGTAGTACAACGTATTGGTATCGCTTTTTCTGGCGGTCTgg                                                                                                                                             >  1:5209241/1‑150 (MQ=255)
       aaaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGac                                                                                                                                           >  2:289853/1‑150 (MQ=255)
       aaaCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGac                                                                                                                                           >  2:3107772/1‑150 (MQ=255)
         aCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGacac                                                                                                                                         <  2:899964/150‑1 (MQ=255)
         aCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGacac                                                                                                                                         <  1:3184617/150‑1 (MQ=255)
         aCTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGacac                                                                                                                                         <  2:4954204/150‑1 (MQ=255)
          cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTCTTGGTATCGCTTTTTCTGGAGGTCTGGACAcc                                                                                                                                        >  1:1036446/1‑150 (MQ=255)
          cTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACAcc                                                                                                                                        >  1:1386442/1‑150 (MQ=255)
            cATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAg                                                                                                                                      <  2:4936810/150‑1 (MQ=255)
            cATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAg                                                                                                                                      >  2:2049474/1‑150 (MQ=255)
             atttatttTGCATAAAAATTCCGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGt                                                                                                                                     <  2:2738590/150‑1 (MQ=255)
             atttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGt                                                                                                                                     >  1:898602/1‑150 (MQ=255)
              tttatttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTg                                                                                                                                    >  1:513854/1‑150 (MQ=255)
                 atttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCg                                                                                                                                 >  2:1915015/1‑150 (MQ=255)
                 atttTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCg                                                                                                                                 >  1:2102328/1‑150 (MQ=255)
                    ttGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCAc                                                                                                                              <  2:2029887/150‑1 (MQ=255)
                     tGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACTATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCAGGACACCAGTGCCGCAct                                                                                                                             >  2:2410373/1‑150 (MQ=255)
                     tGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCAct                                                                                                                             >  1:2417710/1‑150 (MQ=255)
                     tGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCAct                                                                                                                             <  1:1164040/150‑1 (MQ=255)
                            aaaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTTTGGACACCAGTGCCGCACTGCTGTgg                                                                                                                      >  1:1458456/1‑150 (MQ=255)
                             aaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGa                                                                                                                     >  2:2567185/1‑150 (MQ=255)
                               ttCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATg                                                                                                                   <  1:4102658/150‑1 (MQ=255)
                                 cAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCg                                                                                                                 >  1:1927756/1‑150 (MQ=255)
                                 cAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCg                                                                                                                 >  1:1192443/1‑150 (MQ=255)
                                  aGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGa                                                                                                                >  1:4659597/1‑150 (MQ=255)
                                  aGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGa                                                                                                                >  1:1806967/1‑150 (MQ=255)
                                     gagaGCGGAACTCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACaa                                                                                                             <  2:5328267/150‑1 (MQ=255)
                                     gagaGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACaa                                                                                                             >  2:4575819/1‑150 (MQ=255)
                                        agCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAg                                                                                                          >  2:4865623/1‑150 (MQ=255)
                                          cGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAggg                                                                                                        <  1:3941169/150‑1 (MQ=255)
                                           ggAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCACGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGa                                                                                                       <  1:537350/150‑1 (MQ=255)
                                           ggAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGa                                                                                                       >  1:1070457/1‑150 (MQ=255)
                                                 ccAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGtt                                                                                                 >  2:2979444/1‑150 (MQ=255)
                                                   aGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTcc                                                                                               <  1:695881/150‑1 (MQ=255)
                                                   aGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTcc                                                                                               >  2:2117699/1‑150 (MQ=255)
                                                       tcatcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTAt                                                                                           <  2:3658296/150‑1 (MQ=255)
                                                        catcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATg                                                                                          >  1:437268/1‑150 (MQ=255)
                                                        catcatcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATg                                                                                          <  1:1555070/150‑1 (MQ=255)
                                                           catcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCat                                                                                       >  2:793408/1‑150 (MQ=255)
                                                           catcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCat                                                                                       >  2:1730268/1‑150 (MQ=255)
                                                            atcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCata                                                                                      >  2:3608149/1‑150 (MQ=255)
                                                            atcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCata                                                                                      >  1:1396631/1‑150 (MQ=255)
                                                            atcaGTTAATAAAGCAGGGTGTTATTTTATTACTACGATTCTCAAGCATCTCCCGTTAGGACAACGTATTGGTATCTCTATTTATGGAGGTCTGGACACAATTGCCGCACTGCTGTTGATGCGACAAAAGTGATCGTTTCATTATGCata                                                                                      >  2:3510941/1‑150 (MQ=255)
                                                               aGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATAc                                                                                   <  2:3223337/150‑1 (MQ=255)
                                                                gTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACt                                                                                  >  2:4981020/1‑150 (MQ=255)
                                                                       aaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAAcc                                                                           <  2:706958/150‑1 (MQ=255)
                                                                       aaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAAcc                                                                           <  1:1303980/150‑1 (MQ=255)
                                                                          cAGGGTGTTATTTTATGACGACGATTCTCAAGCATATCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTgg                                                                        >  2:4110301/1‑150 (MQ=255)
                                                                           aGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTggg                                                                       <  2:1357969/150‑1 (MQ=255)
                                                                           aGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTggg                                                                       >  2:3624519/1‑150 (MQ=255)
                                                                               tgtTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGgccag                                                                   >  1:4060909/1‑150 (MQ=255)
                                                                               tgtTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTATTACTGGAGGTCTGGACACCAGTGCCGCACTGCTGAGGATGCGACAAAAGGGAGCGGTTCCTAATGCATATACAGCAAACCTGGgccag                                                                   >  2:2682935/1‑150 (MQ=255)
                                                                                  tATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGgccagcca                                                                <  2:3489924/150‑1 (MQ=255)
                                                                                  tATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGgccagcca                                                                <  2:4506772/150‑1 (MQ=255)
                                                                                   aTTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGccagccag                                                               >  1:3528368/1‑150 (MQ=255)
                                                                                   aTTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGccagccag                                                               >  1:2835840/1‑150 (MQ=255)
                                                                                     tttATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGAc                                                             <  2:832758/150‑1 (MQ=255)
                                                                                        aTGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGaa                                                          <  2:3023589/150‑1 (MQ=255)
                                                                                          gacgacGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAaga                                                        <  1:977200/150‑1 (MQ=255)
                                                                                           acgacgATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAagag                                                       <  1:5281039/150‑1 (MQ=255)
                                                                                           acgacgATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAagag                                                       <  2:1504621/150‑1 (MQ=255)
                                                                                             gacgaTTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGa                                                     <  1:5095420/150‑1 (MQ=255)
                                                                                                   tctcAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGTGACAAAAGTGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatga                                               >  2:4249809/1‑150 (MQ=255)
                                                                                                    ctcAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgat                                              >  1:1652835/1‑150 (MQ=255)
                                                                                                    ctcAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgat                                              >  1:3570967/1‑150 (MQ=255)
                                                                                                    ctcAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgat                                              <  2:1819791/150‑1 (MQ=255)
                                                                                                     tcAAGCATCTCNCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgatg                                             >  1:2765538/1‑150 (MQ=255)
                                                                                                     tcAAGCATCTCCCGTTAGGTCTACGTATTGGTATCTCTTTTTCTGGAGGTATGGACACCAGTGACGCACTGCTGTGGATGCGACAAAATGGAGCGGTTCCTTATGCATATAATGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgatg                                             <  1:4219558/150‑1 (MQ=255)
                                                                                                      cAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGc                                            >  2:3892025/1‑150 (MQ=255)
                                                                                                      cAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGc                                            <  1:4969624/150‑1 (MQ=255)
                                                                                                      cAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGc                                            <  2:1305295/150‑1 (MQ=255)
                                                                                                       aaGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCg                                           <  2:3701768/150‑1 (MQ=255)
                                                                                                        aGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGa                                          >  2:1860034/1‑150 (MQ=255)
                                                                                                         gCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGAt                                         <  1:3379395/150‑1 (MQ=255)
                                                                                                           aTCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATcc                                       <  1:3825608/150‑1 (MQ=255)
                                                                                                            tctcCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATccc                                      <  1:66140/150‑1 (MQ=255)
                                                                                                                   gTAGGTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTcgtcgt                               >  2:4402479/1‑150 (MQ=255)
                                                                                                                       gTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGACGAGGATTATGATGCGATCCCTCGTCGTGCCa                           >  2:4635713/1‑150 (MQ=255)
                                                                                                                       gTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCa                           <  1:728300/150‑1 (MQ=255)
                                                                                                                       gTCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCa                           >  2:2146905/1‑150 (MQ=255)
                                                                                                                        tCAACGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCAt                          >  1:2648016/1‑150 (MQ=255)
                                                                                                                          aaCGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATgg                        >  2:2495520/1‑150 (MQ=255)
                                                                                                                          aaCGTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATgg                        >  2:4590122/1‑150 (MQ=255)
                                                                                                                             gTATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCACGTCGTGCCATGGAAt                     >  1:2204989/1‑150 (MQ=255)
                                                                                                                              tATTGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATa                    >  2:1200/1‑150 (MQ=255)
                                                                                                                                ttGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACg                  <  2:4042123/150‑1 (MQ=255)
                                                                                                                                 tGGTATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATAGACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACgg                 <  1:2514189/150‑1 (MQ=255)
                                                                                                                                    tATCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGgcgc              >  2:2775046/1‑150 (MQ=255)
                                                                                                                                     aTCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGcgcg             <  1:2897119/150‑1 (MQ=255)
                                                                                                                                     aTCGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGcgcg             >  2:4100398/1‑150 (MQ=255)
                                                                                                                                       cGCTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGga           >  2:5127050/1‑150 (MQ=255)
                                                                                                                                         cTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGgaga         >  1:2452827/1‑150 (MQ=255)
                                                                                                                                         cTTTTTCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGgaga         >  1:4561970/1‑150 (MQ=255)
                                                                                                                                          tttttCTGGAGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAAACTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGGAGaa        <  2:4801869/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAAATGAAAACTCATTTATTTTGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCGCGGAGAACGCACG  >  CP009273/3311908‑3312201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑