Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,317,805 C→A 73.2% D156Y (GAT→TAT)  folP ← 7,8‑dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,317,8050CA73.2% 384.2 / 257.7 299D156Y (GAT→TAT) folP7,8‑dihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (46/34);  new base A (118/101);  total (164/135)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 299 total aligned reads displayed.

CCAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCT  >  CP009273/3317658‑3317953
                                                                                                                                                   |                                                                                                                                                    
ccAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGt                                                                                                                                                    <  2:4315288/150‑1 (MQ=255)
 cAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTc                                                                                                                                                   >  2:2065087/1‑150 (MQ=255)
  aGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGTGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATATGCTCAATAAAGTAGCGATACACTTCTGCAAAGACATAGTCa                                                                                                                                                  >  1:4740696/1‑150 (MQ=255)
  aGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCa                                                                                                                                                  >  2:3238257/1‑150 (MQ=255)
        gcCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTc                                                                                                                                            <  2:1171581/150‑1 (MQ=255)
          cAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCgg                                                                                                                                          >  1:3458877/1‑150 (MQ=255)
           aGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGa                                                                                                                                         >  1:1008248/1‑150 (MQ=255)
           aGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGa                                                                                                                                         >  2:772796/1‑150 (MQ=255)
              aaTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCt                                                                                                                                      <  2:1263630/150‑1 (MQ=255)
               aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCtt                                                                                                                                     <  2:194082/150‑1 (MQ=255)
               aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCtt                                                                                                                                     <  2:1748697/150‑1 (MQ=255)
               aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCtt                                                                                                                                     >  2:344481/1‑150 (MQ=255)
               aTGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCtt                                                                                                                                     >  2:2971499/1‑150 (MQ=255)
                tGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTc                                                                                                                                    >  1:1047157/1‑150 (MQ=255)
                 gAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTcc                                                                                                                                   <  1:3685176/150‑1 (MQ=255)
                  aaTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCt                                                                                                                                  >  2:3459217/1‑150 (MQ=255)
                   aTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTg                                                                                                                                 >  1:4299938/1‑150 (MQ=255)
                   aTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTg                                                                                                                                 >  1:5291275/1‑150 (MQ=255)
                   aTAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGAGATTCACTTCTGCAAAGACATAGTCAAACTACGGAGCTTCCTg                                                                                                                                 >  1:1472582/1‑150 (MQ=255)
                        tATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATgg                                                                                                                            <  2:15999/150‑1 (MQ=255)
                           gggAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGttt                                                                                                                         >  2:252878/1‑150 (MQ=255)
                           gggAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGttt                                                                                                                         >  1:271995/1‑150 (MQ=255)
                            ggAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGtttt                                                                                                                        >  2:4064187/1‑150 (MQ=255)
                            ggAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGtttt                                                                                                                        >  1:3104819/1‑150 (MQ=255)
                                 gaTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGAt                                                                                                                   <  2:2873397/150‑1 (MQ=255)
                                  aTTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGAtt                                                                                                                  <  1:2971499/150‑1 (MQ=255)
                                       tACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCt                                                                                                             <  1:983920/150‑1 (MQ=255)
                                        accgaaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTg                                                                                                            >  2:4667486/1‑150 (MQ=255)
                                          cgaaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCa                                                                                                          >  2:3613500/1‑150 (MQ=255)
                                           gaaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCat                                                                                                         >  2:2408604/1‑150 (MQ=255)
                                           gaaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTGTTGGATTTCCCTGCat                                                                                                         >  2:491331/1‑150 (MQ=255)
                                            aaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCata                                                                                                        >  1:826833/1‑150 (MQ=255)
                                            aaaccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCata                                                                                                        >  2:4130864/1‑150 (MQ=255)
                                             caccgaaTCCGGGGTCGAGCCACAATGTCTCTTGTGCGAGACCCGCCTGCTCGCAACGTGCTATTTGCTCAATACAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCatat                                                                                                       <  1:2378218/149‑1 (MQ=255)
                                              accgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATg                                                                                                      >  2:2803948/1‑150 (MQ=255)
                                               ccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGc                                                                                                     >  1:1255501/1‑150 (MQ=255)
                                               ccgaaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGc                                                                                                     >  2:1374039/1‑150 (MQ=255)
                                                  aaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATc                                                                                                  >  1:190253/1‑150 (MQ=255)
                                                  aaTCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATc                                                                                                  >  2:4743970/1‑150 (MQ=255)
                                                        gggTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaa                                                                                            <  1:951803/150‑1 (MQ=255)
                                                          gTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaac                                                                                          <  1:2175319/150‑1 (MQ=255)
                                                          gTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaac                                                                                          <  1:5084646/150‑1 (MQ=255)
                                                           tCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaacc                                                                                         <  2:390521/150‑1 (MQ=255)
                                                            cGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccg                                                                                        <  1:1996432/150‑1 (MQ=255)
                                                            cGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccg                                                                                        >  1:1386473/1‑150 (MQ=255)
                                                            agAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccg                                                                                        <  2:588368/149‑1 (MQ=255)
                                                             gAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccgg                                                                                       >  1:5141515/1‑150 (MQ=255)
                                                              aGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggt                                                                                      >  1:1417234/1‑150 (MQ=255)
                                                                 aacaaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtaaa                                                                                   >  2:2011850/1‑150 (MQ=255)
                                                                    aaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtaaaccg                                                                                >  1:1143018/1‑150 (MQ=255)
                                                                    aaTTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACaaaccggtaaaccg                                                                                >  1:2409769/1‑150 (MQ=255)
                                                                       ttCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGtt                                                                             <  2:1558341/150‑1 (MQ=255)
                                                                         ctctTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTc                                                                           >  2:453527/1‑150 (MQ=255)
                                                                            ttttGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGc                                                                        <  1:1813411/150‑1 (MQ=255)
                                                                             tttGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCa                                                                       <  2:4403272/150‑1 (MQ=255)
                                                                                  gATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAgccgc                                                                  >  1:4474689/1‑150 (MQ=255)
                                                                                    tACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCt                                                                <  1:2401712/150‑1 (MQ=255)
                                                                                    tACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACGTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCt                                                                <  2:4012040/150‑1 (MQ=255)
                                                                                        cGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCag                                                            <  1:3354994/150‑1 (MQ=255)
                                                                                        cGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCag                                                            <  1:3413791/150‑1 (MQ=255)
                                                                                        cGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCag                                                            >  1:1570339/1‑150 (MQ=255)
                                                                                                cGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAg                                                    <  1:1352064/150‑1 (MQ=255)
                                                                                                 gCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAgg                                                   <  2:1649258/150‑1 (MQ=255)
                                                                                                 gCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAgg                                                   <  1:5197604/150‑1 (MQ=255)
                                                                                                   aaCGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGTTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGtt                                                 <  2:950915/150‑1 (MQ=255)
                                                                                                     cGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCg                                               >  2:279656/1‑150 (MQ=255)
                                                                                                         cTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTCAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGaaa                                           >  2:3368862/1‑150 (MQ=255)
                                                                                                          tATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAg                                          >  1:415191/1‑150 (MQ=255)
                                                                                                            tttGCTCAATAAAGTAGCGAGTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAggg                                        <  2:1432815/150‑1 (MQ=255)
                                                                                                              tGCTCACTAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAg                                      <  1:4564282/150‑1 (MQ=255)
                                                                                                              tGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAg                                      <  2:4885447/150‑1 (MQ=255)
                                                                                                                cTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTCAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCg                                    >  2:5130320/1‑150 (MQ=255)
                                                                                                                cTCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCg                                    <  1:4645245/150‑1 (MQ=255)
                                                                                                                 tCAATAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCgg                                   >  2:2237373/1‑150 (MQ=255)
                                                                                                                   aaTAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTCAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGat                                 >  1:3971647/1‑150 (MQ=255)
                                                                                                                     tAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGatat                               <  2:5214054/150‑1 (MQ=255)
                                                                                                                     tAAAGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGatat                               >  2:4559531/1‑150 (MQ=255)
                                                                                                                      aaaGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATc                              <  1:3238257/150‑1 (MQ=255)
                                                                                                                      aaaGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATc                              >  2:1856628/1‑150 (MQ=255)
                                                                                                                        aGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                            >  2:3727494/1‑150 (MQ=255)
                                                                                                                        aGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTCAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                            >  2:1515374/1‑150 (MQ=255)
                                                                                                                        aGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                            >  1:307962/1‑150 (MQ=255)
                                                                                                                        aGTAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCAt                            <  2:180931/150‑1 (MQ=255)
                                                                                                                          tAGCGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATta                          <  1:2065087/150‑1 (MQ=255)
                                                                                                                             cGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaata                       >  2:262116/1‑150 (MQ=255)
                                                                                                                             cGATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaata                       >  1:1684299/1‑150 (MQ=255)
                                                                                                                              gATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTCAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                      >  1:5322436/1‑150 (MQ=255)
                                                                                                                              gATTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATtaataa                      >  1:4324818/1‑150 (MQ=255)
                                                                                                                               aTTCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTaataat                     >  1:3846587/1‑150 (MQ=255)
                                                                                                                                ttCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAAtg                    <  1:2674702/150‑1 (MQ=255)
                                                                                                                                 tCACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAAtgt                   <  2:1054502/150‑1 (MQ=255)
                                                                                                                                  cACTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAAtgtg                  <  1:772796/150‑1 (MQ=255)
                                                                                                                                    cTTCTGCAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAg                >  1:4042981/1‑150 (MQ=255)
                                                                                                                                          cAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCaa          >  1:2523286/1‑150 (MQ=255)
                                                                                                                                          cAAAGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCaa          >  1:358945/1‑150 (MQ=255)
                                                                                                                                           aaaGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAAc         >  2:4501042/1‑150 (MQ=255)
                                                                                                                                           aaaGACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAAc         >  2:4688185/1‑150 (MQ=255)
                                                                                                                                              gACATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACttt      >  2:2110297/1‑150 (MQ=255)
                                                                                                                                               aCATAGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCACTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCATGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTc     <  2:4258022/150‑1 (MQ=255)
                                                                                                                                                 aTCGTCATACTACGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGTTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGAGGATATCATTCATAATGTGAGCGCCAACTTTCGc   >  2:2305628/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCAGGCGCGCCAGTAATGAATAGTTATGGGAGAGATTTTTACCGAAACCGAATCCGGGGTCGAGCAACAATTTCTCTTTTGCGATACCCGCCTGCTCGCAACGTGCTATTTGCTCAATAAAGTAGCGATTCACTTCTGCAAAGACATCGTCATACTTCGGAGCTTCCTGCATGGTTTTTGGATTTCCCTGCATATGCATCAGACAAACCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCT  >  CP009273/3317658‑3317953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑