Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,332,963 C→A 100% A154A (GCG→GCT)  mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,332,9630CA98.7% 977.8 / 2.3 303A154A (GCG→GCT) mlaFABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (154/145);  total (157/146)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 306 total aligned reads displayed.

GTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGAC  >  CP009273/3332816‑3333109
                                                                                                                                                   |                                                                                                                                                  
 tGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCt                                                                                                                                                 <  1:4882288/150‑1 (MQ=255)
 tGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCt                                                                                                                                                 <  2:5098248/150‑1 (MQ=255)
   agaAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGc                                                                                                                                               >  2:245869/1‑150 (MQ=255)
     aaaCCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAATGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCAc                                                                                                                                             >  2:4720714/1‑150 (MQ=255)
     aaaCCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCAc                                                                                                                                             >  2:1432955/1‑150 (MQ=255)
     aaaCCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCAc                                                                                                                                             <  1:374416/150‑1 (MQ=255)
        ccaccaCACAAGTCACGCCCAGCGCGCTGTTCAGCTCATAAATCAGCTTCACCTGGACGCCCATGGTGATGGGATCTTGCCCAACATAAGGTTAATCAAACATGATGAGATCCGGCTACAGCGCAATCGCACGCGCCAGAGCTGCAcgcc                                                                                                                                          <  1:2739413/150‑1 (MQ=255)
         caccacACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCAcgccg                                                                                                                                         <  2:2740899/150‑1 (MQ=255)
            cacacaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCgcgc                                                                                                                                      >  2:4886603/1‑150 (MQ=255)
            cacacaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGACCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCAgcgc                                                                                                                                      >  1:980620/1‑150 (MQ=255)
             acacaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGcc                                                                                                                                     <  2:1662964/150‑1 (MQ=255)
              cacaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCa                                                                                                                                    <  1:1338393/150‑1 (MQ=255)
              cacaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCa                                                                                                                                    >  2:3399684/1‑150 (MQ=255)
               acaAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCAt                                                                                                                                   >  1:1141659/1‑150 (MQ=255)
                caAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATc                                                                                                                                  <  2:2013048/150‑1 (MQ=255)
                caAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATc                                                                                                                                  >  1:2679385/1‑150 (MQ=255)
                 aaGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATcc                                                                                                                                 >  1:2347120/1‑150 (MQ=255)
                   gTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCa                                                                                                                               <  2:529258/150‑1 (MQ=255)
                     cACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCAcc                                                                                                                             >  1:1385920/1‑150 (MQ=255)
                     cACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCAcc                                                                                                                             <  2:3967019/150‑1 (MQ=255)
                           cAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAg                                                                                                                       <  2:1240976/150‑1 (MQ=255)
                             gcgcgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGtt                                                                                                                     <  1:4489540/150‑1 (MQ=255)
                              cgcgcTGTTCAGCTCAGAACTCAGCTTCACCCGTACGCCCAGGGTGATGGGATCTTGCCCAACAAACGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTc                                                                                                                    <  2:4113951/150‑1 (MQ=255)
                              cgcgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTc                                                                                                                    >  1:2187632/1‑150 (MQ=255)
                               gcgcTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCa                                                                                                                   >  1:514357/1‑150 (MQ=255)
                                    gTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAgg                                                                                                              <  2:791333/150‑1 (MQ=255)
                                           tCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGt                                                                                                       <  2:4980756/150‑1 (MQ=255)
                                            cAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAGAGTTCAGAAGGCATTAGtt                                                                                                      >  2:1756067/1‑150 (MQ=255)
                                               aaaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAg                                                                                                   <  1:2274000/150‑1 (MQ=255)
                                               aaaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAg                                                                                                   <  2:5170298/150‑1 (MQ=255)
                                                aaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGc                                                                                                  >  1:314561/1‑150 (MQ=255)
                                                aaTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGc                                                                                                  >  2:4408026/1‑150 (MQ=255)
                                                 aTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGcc                                                                                                 <  1:3394139/150‑1 (MQ=255)
                                                 aTCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGcc                                                                                                 >  2:3555285/1‑150 (MQ=255)
                                                    aGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCt                                                                                              >  1:3820389/1‑150 (MQ=255)
                                                    aGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCt                                                                                              <  1:1923042/150‑1 (MQ=255)
                                                      cTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTcc                                                                                            <  1:69255/150‑1 (MQ=255)
                                                           ccAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCa                                                                                       <  1:11198/150‑1 (MQ=255)
                                                                aCGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGcccc                                                                                  >  2:2952610/1‑150 (MQ=255)
                                                                 cGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCa                                                                                 <  1:4794169/150‑1 (MQ=255)
                                                                       tGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCt                                                                           <  1:2555665/150‑1 (MQ=255)
                                                                       tGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCt                                                                           >  1:1887198/1‑150 (MQ=255)
                                                                          tGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCa                                                                        <  2:603487/150‑1 (MQ=255)
                                                                          tGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCa                                                                        <  2:3791722/150‑1 (MQ=255)
                                                                             tGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCt                                                                     >  2:3887501/1‑150 (MQ=255)
                                                                             tGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCt                                                                     <  2:4571004/150‑1 (MQ=255)
                                                                               ggATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtc                                                                   >  1:2886232/1‑150 (MQ=255)
                                                                               ggATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtc                                                                   <  1:1675760/150‑1 (MQ=255)
                                                                                gATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtca                                                                  <  1:4384837/150‑1 (MQ=255)
                                                                                  tCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatc                                                                <  2:5007400/150‑1 (MQ=255)
                                                                                   cTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatca                                                               <  1:3277790/150‑1 (MQ=255)
                                                                                      gCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTtcatcatca                                                            >  1:416613/1‑150 (MQ=255)
                                                                                       cccAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCAc                                                           >  2:321845/1‑150 (MQ=255)
                                                                                         caacaaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCg                                                         >  1:5200611/1‑150 (MQ=255)
                                                                                            caaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTAc                                                      <  1:5277385/150‑1 (MQ=255)
                                                                                            caaAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTAc                                                      >  1:2829283/1‑150 (MQ=255)
                                                                                              aaaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGGTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTa                                                    >  2:699884/1‑150 (MQ=255)
                                                                                               aaGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTAt                                                   >  1:3512713/1‑150 (MQ=255)
                                                                                                aGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATg                                                  <  2:4508156/150‑1 (MQ=255)
                                                                                                 ggTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGc                                                 >  2:2163371/1‑150 (MQ=255)
                                                                                                 ggTTCATCAAACATGATGAGAACCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTACATCATCACCGTACTATGc                                                 >  1:162204/1‑150 (MQ=255)
                                                                                                      atcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGcaacaa                                            >  2:3253961/1‑150 (MQ=255)
                                                                                                       tcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAAt                                           <  1:198092/150‑1 (MQ=255)
                                                                                                       tcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAAt                                           >  1:4347424/1‑150 (MQ=255)
                                                                                                       tcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAAt                                           <  2:1918137/150‑1 (MQ=255)
                                                                                                       tcaAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAAt                                           >  2:169741/1‑150 (MQ=255)
                                                                                                          aaCATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgc                                        <  1:4965304/150‑1 (MQ=255)
                                                                                                          aaCATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgc                                        <  2:1491786/150‑1 (MQ=255)
                                                                                                           aCATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgcg                                       <  2:3082896/150‑1 (MQ=255)
                                                                                                           aCATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgcg                                       >  2:3150791/1‑150 (MQ=255)
                                                                                                            cATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGgcgc                                      >  2:4989057/1‑150 (MQ=255)
                                                                                                            aatgatgAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTATAGCCGCTCAACGCAGCCCCACGGCCTCCAGCTTCATCATCAACGTACTATGCAACAATGgcgc                                      >  1:2519036/2‑150 (MQ=255)
                                                                                                             atgatgAGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGcgcg                                     <  1:5324795/150‑1 (MQ=255)
                                                                                                                atgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGa                                  >  2:2692806/1‑150 (MQ=255)
                                                                                                                 tgaGATCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTTGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGaa                                 <  1:203942/150‑1 (MQ=255)
                                                                                                                  gagaTCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAg                                <  1:3974189/150‑1 (MQ=255)
                                                                                                                    gaTCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGtt                              >  1:5296506/1‑150 (MQ=255)
                                                                                                                    gaTCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGtt                              >  1:4086557/1‑150 (MQ=255)
                                                                                                                     aTCCGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTg                             <  1:2487020/150‑1 (MQ=255)
                                                                                                                       ccGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTggg                           <  2:1377663/150‑1 (MQ=255)
                                                                                                                        cGGCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCTCCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGACGTTGGGt                          >  2:5152193/1‑150 (MQ=255)
                                                                                                                         ggCTCCAGCGCAATAGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTa                         <  2:3707089/150‑1 (MQ=255)
                                                                                                                          gCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                        <  1:5011415/150‑1 (MQ=255)
                                                                                                                          gCTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAt                        <  2:4386910/150‑1 (MQ=255)
                                                                                                                           cTCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATg                       >  2:2799310/1‑150 (MQ=255)
                                                                                                                            tCCAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTAAGt                      <  2:1795435/150‑1 (MQ=255)
                                                                                                                            tCCAGCGCAATCACACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGt                      >  1:2109495/1‑150 (MQ=255)
                                                                                                                              cAGCGCAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTc                    <  2:2384230/150‑1 (MQ=255)
                                                                                                                                gcgcAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcgc                  >  1:4577727/1‑150 (MQ=255)
                                                                                                                                 cgcAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTcgcg                 <  2:1115409/150‑1 (MQ=255)
                                                                                                                                  gcAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCgcgc                >  1:787189/1‑150 (MQ=255)
                                                                                                                                  gcAATCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCgcgc                >  1:584086/1‑150 (MQ=255)
                                                                                                                                      tCGCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTg            <  1:2700708/150‑1 (MQ=255)
                                                                                                                                        gCACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGa          >  1:3681603/1‑150 (MQ=255)
                                                                                                                                         cACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAt         >  1:1784951/1‑150 (MQ=255)
                                                                                                                                         cACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAt         >  1:389339/1‑150 (MQ=255)
                                                                                                                                         cACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAt         >  2:3307314/1‑150 (MQ=255)
                                                                                                                                         cACGCGCCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAt         <  1:1458907/150‑1 (MQ=255)
                                                                                                                                             cgcCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGc     <  1:1006363/150‑1 (MQ=255)
                                                                                                                                              gcCAGAGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCg    <  2:1646953/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGAGAAACCACCACACAAGTCACGCCCAGCGCGCTGTTCAGCTCAGAAATCAGCTTCACCAGTACGCCCATGGTGATGGGATCTTGCCCAACAAAAGGTTCATCAAACATGATGAGATCCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGAC  >  CP009273/3332816‑3333109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑