BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,355,202	T→A	6.7%	L223* (TTA→TAA)	gltF →	periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,355,202	0	T	A	6.7%	1088.6 / 46.4	353	L223* (TTA→TAA)	gltF	periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (161/168); new base A (13/11); total (174/179)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

CACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTC  >  CP009273/3355054‑3355351

  cTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACt                                                                                                                                                    <  1:5267603/150‑1 (MQ=255)

   tGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACtg                                                                                                                                                   >  2:245166/1‑150 (MQ=255)

        agcagcGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTAACtgattga                                                                                                                                              >  2:860179/1‑150 (MQ=255)

         gcagcGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACtgattgat                                                                                                                                             <  2:3980455/150‑1 (MQ=255)

          cagcGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGAtg                                                                                                                                            <  2:4139694/150‑1 (MQ=255)

              gACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGAtgctgc                                                                                                                                        <  2:1915998/150‑1 (MQ=255)

               aCCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCGATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCg                                                                                                                                       >  2:5040498/1‑150 (MQ=255)

                  cccACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTa                                                                                                                                    >  1:2716955/1‑150 (MQ=255)

                  cccACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTa                                                                                                                                    >  1:3423786/1‑150 (MQ=255)

                  cccACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTa                                                                                                                                    <  2:3811691/150‑1 (MQ=255)

                   ccACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTaa                                                                                                                                   >  1:1151483/1‑150 (MQ=255)

                   ccACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTaa                                                                                                                                   <  1:1581042/150‑1 (MQ=255)

                    cacaTGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTaaa                                                                                                                                  >  1:1973198/1‑150 (MQ=255)

                         ggAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACa                                                                                                                             <  2:5170968/150‑1 (MQ=255)

                          gAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACaa                                                                                                                            >  1:1029022/1‑150 (MQ=255)

                            aaGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATa                                                                                                                          >  1:4231863/1‑150 (MQ=255)

                             aGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATAc                                                                                                                         <  1:2771566/150‑1 (MQ=255)

                                  cATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTc                                                                                                                    >  2:4736537/1‑150 (MQ=255)

                                    tCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCtt                                                                                                                  <  2:4076421/150‑1 (MQ=255)

                                     cTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTg                                                                                                                 <  1:4834891/150‑1 (MQ=255)

                                     cTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTg                                                                                                                 >  2:233204/1‑150 (MQ=255)

                                       aaTGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGt                                                                                                               <  2:2522412/150‑1 (MQ=255)

                                             gcATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCtgat                                                                                                         <  1:2516779/150‑1 (MQ=255)

                                             gcATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCtgat                                                                                                         <  2:1578614/150‑1 (MQ=255)

                                              cATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCtgatg                                                                                                        >  1:3333533/1‑150 (MQ=255)

                                                  cTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAAc                                                                                                    <  1:1387598/150‑1 (MQ=255)

                                                  cTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAAc                                                                                                    >  1:1229668/1‑150 (MQ=255)

                                                   tGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACg                                                                                                   <  2:1687580/150‑1 (MQ=255)

                                                     cTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGAt                                                                                                 >  1:967579/1‑150 (MQ=255)

                                                      tCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATc                                                                                                <  2:2797299/150‑1 (MQ=255)

                                                      tCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATc                                                                                                <  1:3883590/150‑1 (MQ=255)

                                                        gCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGGTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACACTACTATTCTTGGTTCTGATGAAACGATCaa                                                                                              >  2:563520/1‑150 (MQ=255)

                                                          tGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAAc                                                                                            >  2:4442344/1‑150 (MQ=255)

                                                            aCAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTg                                                                                          >  1:1638442/1‑150 (MQ=255)

                                                                gTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATg                                                                                      <  1:2864308/150‑1 (MQ=255)

                                                                 ttGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATgg                                                                                     >  2:4866878/1‑150 (MQ=255)

                                                                    cTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTaa                                                                                  <  1:1342411/150‑1 (MQ=255)

                                                                       aGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGt                                                                               >  2:3297767/1‑150 (MQ=255)

                                                                         cAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCa                                                                             >  1:4725170/1‑150 (MQ=255)

                                                                          aaTCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCAc                                                                            >  2:3699048/1‑150 (MQ=255)

                                                                           aTCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACt                                                                           >  1:564889/1‑150 (MQ=255)

                                                                              gAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATc                                                                        <  1:1880528/150‑1 (MQ=255)

                                                                              gAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATc                                                                        >  1:5051966/1‑150 (MQ=255)

                                                                              gAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTAACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATc                                                                        >  2:2675405/1‑150 (MQ=255)

                                                                               aGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCa                                                                       <  1:1904487/150‑1 (MQ=255)

                                                                                 cgcgGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGc                                                                     >  1:2308072/1‑150 (MQ=255)

                                                                                    ggTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGtt                                                                  >  2:119193/1‑150 (MQ=255)

                                                                                    ggTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGtt                                                                  <  2:4256269/150‑1 (MQ=255)

                                                                                    ggTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGtt                                                                  >  1:775416/1‑150 (MQ=255)

                                                                                    ggTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGtt                                                                  >  1:1079150/1‑150 (MQ=255)

                                                                                    ggTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGtt                                                                  >  1:229286/1‑150 (MQ=255)

                                                                                     gTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTAACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTc                                                                 >  2:3043560/1‑150 (MQ=255)

                                                                                      ttATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCa                                                                <  2:655655/150‑1 (MQ=255)

                                                                                      atATTCTGTTGCAAAAACAGTGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCa                                                                <  2:3537447/149‑1 (MQ=255)

                                                                                             gTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTta                                                         >  1:316383/1‑150 (MQ=255)

                                                                                             gTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTta                                                         >  2:1616693/1‑150 (MQ=255)

                                                                                              ttGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTtat                                                        >  1:1495326/1‑150 (MQ=255)

                                                                                               tGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTtata                                                       <  2:3597528/150‑1 (MQ=255)

                                                                                                     aaCAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACt                                                 <  1:2232255/150‑1 (MQ=255)

                                                                                                        aGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGt                                              >  1:4598392/1‑150 (MQ=255)

                                                                                                        aGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGt                                              <  1:2038431/150‑1 (MQ=255)

                                                                                                        aGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTAACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGt                                              >  2:1864860/1‑150 (MQ=255)

                                                                                                         ggggAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTa                                             >  2:376244/1‑150 (MQ=255)

                                                                                                         ggggAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTa                                             >  2:1789169/1‑150 (MQ=255)

                                                                                                          gggAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTac                                            >  1:5082844/1‑149 (MQ=255)

                                                                                                           ggAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAAt                                           <  2:3142702/150‑1 (MQ=255)

                                                                                                            gAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATa                                          <  2:2098245/150‑1 (MQ=255)

                                                                                                             aaCTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATAc                                         >  2:109542/1‑150 (MQ=255)

                                                                                                              aCTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACa                                        <  2:2260690/150‑1 (MQ=255)

                                                                                                                tGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGt                                      >  2:537009/1‑150 (MQ=255)

                                                                                                                tGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGt                                      >  1:4132338/1‑150 (MQ=255)

                                                                                                                 gACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGtt                                     <  2:1337472/150‑1 (MQ=255)

                                                                                                                  acacCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGttt                                    <  2:3346076/150‑1 (MQ=255)

                                                                                                                  acacCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGttt                                    >  1:4680742/1‑150 (MQ=255)

                                                                                                                  acacCTGTTGATATTACAGCGGTCAACTTCCCGTTACTGATTGATGCTGCGGTAAATGACATTACTATTCTTGTTTCTGATGAAACTATCAAACTGGATGTTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGttt                                    <  1:4466530/150‑1 (MQ=255)

                                                                                                                   cacCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGtttt                                   <  2:1742446/150‑1 (MQ=255)

                                                                                                                     ccTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGttttta                                 <  1:266630/150‑1 (MQ=255)

                                                                                                                      cTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGtttttat                                >  1:4783239/1‑150 (MQ=255)

                                                                                                                        gTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGtttttattt                              >  2:2036540/1‑150 (MQ=255)

                                                                                                                        gTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGtttttattt                              <  2:3904053/150‑1 (MQ=255)

                                                                                                                         ttGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACTATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGtttttatttt                             <  2:2705344/150‑1 (MQ=255)

                                                                                                                          tGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGtttttattttt                            >  1:1784754/1‑150 (MQ=255)

                                                                                                                            cTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTAc                          <  1:1676627/150‑1 (MQ=255)

                                                                                                                             tATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACg                         >  2:5250841/1‑150 (MQ=255)

                                                                                                                              aTTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGc                        >  1:894039/1‑150 (MQ=255)

                                                                                                                              aTTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGc                        >  1:4083487/1‑150 (MQ=255)

                                                                                                                              aTTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGc                        >  1:3996127/1‑150 (MQ=255)

                                                                                                                              aTTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGc                        >  2:4578516/1‑150 (MQ=255)

                                                                                                                                tACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCAt                      <  2:135649/150‑1 (MQ=255)

                                                                                                                                     cGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAAcc                 <  1:3215114/150‑1 (MQ=255)

                                                                                                                                       gTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAg               <  1:245166/150‑1 (MQ=255)

                                                                                                                                       gTCACCTTCCCGTAACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAg               >  1:3089896/1‑150 (MQ=255)

                                                                                                                                         cACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGtt             >  1:3259820/1‑150 (MQ=255)

                                                                                                                                           ccTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAg           >  1:3679712/1‑150 (MQ=255)

                                                                                                                                            cTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGc          >  2:2921150/1‑150 (MQ=255)

                                                                                                                                               cccGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCtat       >  1:3050057/1‑150 (MQ=255)

                                                                                                                                                ccGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCtata      <  2:259438/150‑1 (MQ=255)

                                                                                                                                                 cGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATaa     >  1:2187316/1‑150 (MQ=255)

                                                                                                                                                 cGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATaa     >  1:3597941/1‑150 (MQ=255)

                                                                                                                                                 cGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATaa     >  1:2633541/1‑150 (MQ=255)

CACTGGTCAGCAGCGACCCCCACATGGAAAGCCTCATCTAATGGCGCATTCTGCTCGCTGACAAGTTGCTCAGCAATCGAGCGCGGTTATTCTGTTGCAAAAACAGGGGAACTGACACCTGTTGCTATTACAGCGGTCACCTTCCCGTTACTGATTGATGCTGCGGTAAATGACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTC  >  CP009273/3355054‑3355351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`