Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,384,761 A→C 6.4% G209G (GGT→GGG)  tldD ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,384,7610AC 6.4% 650.1 / 10.3 261G209G (GGT→GGG) tldDputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (175/64);  new base C (2/20);  total (177/84)
Fisher's exact test for biased strand distribution p-value = 3.30e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACT  >  CP009273/3384613‑3384910
                                                                                                                                                    |                                                                                                                                                     
cGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAAc                                                                                                                                                      <  1:5254949/150‑1 (MQ=255)
 gCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAAcc                                                                                                                                                     <  1:4365002/150‑1 (MQ=255)
  cccGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCa                                                                                                                                                    <  2:396906/150‑1 (MQ=255)
   ccGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCaa                                                                                                                                                   >  1:1941457/1‑150 (MQ=255)
      gCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAAc                                                                                                                                                <  2:628371/150‑1 (MQ=255)
         aaCCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGACGAATTCATAACCAAAACGAc                                                                                                                                             >  1:67760/1‑150 (MQ=255)
         aaCCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAc                                                                                                                                             >  1:2097841/1‑150 (MQ=255)
         aaCCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAc                                                                                                                                             >  2:3162069/1‑150 (MQ=255)
                gcCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAccgccgcc                                                                                                                                      >  2:1064192/1‑150 (MQ=255)
                  cAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGACAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGACTTCATAACCAAAACGACcgccgccgc                                                                                                                                    >  2:4638270/1‑150 (MQ=255)
                  cAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACcgccgccgc                                                                                                                                    >  2:2048655/1‑150 (MQ=255)
                   aaGTACTACCGACATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCgccgccgcc                                                                                                                                   >  1:1277654/1‑150 (MQ=255)
                       aatacCGGCTTGGTGCCCGTTGGTGAAGCAACGGAATAAATATTGACCAGCGCCATACGAACTGATTATTTTGCCCAGGCATCCGCACGGACTTCGCGGTCGAGATCGGAAAGGAAGAATTAATAACCAAAACGACCGCCGCCGCCACTg                                                                                                                               <  2:425699/148‑1 (MQ=255)
                         tacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGc                                                                                                                             >  2:1103819/1‑150 (MQ=255)
                         tacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGc                                                                                                                             >  1:362524/1‑150 (MQ=255)
                             ggCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcg                                                                                                                         >  1:3397851/1‑150 (MQ=255)
                                   gTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcg                                                                                                                   >  1:3154825/1‑150 (MQ=255)
                                     gCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCCAAACGACCGCCGCCGCCACTGGCACcgcgttcgcg                                                                                                                 >  1:4825567/1‑150 (MQ=255)
                                           tGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGcc                                                                                                           >  1:4164249/1‑150 (MQ=255)
                                           tGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGcc                                                                                                           >  2:2550212/1‑150 (MQ=255)
                                                cagaaACGGCAGAAAGATTGACCAGCGCCATACGCACTGATTATTTTGCCCATGCATCCGCACGGACTTCGCCGTCTAGATCGGCAAGTAATAATTAATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtctt                                                                                                      <  2:265097/150‑1 (MQ=255)
                                                  gcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtcttct                                                                                                    >  2:893276/1‑150 (MQ=255)
                                                    aaCGGCAGAAAGAATGACCAGCGCCATACGCACTGCTTCTTATGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACTACCGCCGCCGCCACTGGCACCGCGAACGCGTTTGCCATCttcttc                                                                                                  >  1:1777712/1‑150 (MQ=255)
                                                        gCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgacg                                                                                              >  2:2144303/1‑150 (MQ=255)
                                                          aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                            >  2:1175373/1‑150 (MQ=255)
                                                          aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                            >  2:744981/1‑150 (MQ=255)
                                                          aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                            >  1:1455669/1‑150 (MQ=255)
                                                          aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                            >  2:1788822/1‑150 (MQ=255)
                                                             aaGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAAc                                                                                         >  1:1700821/1‑150 (MQ=255)
                                                             aaGATTGACCAGCGCCATACGCACGGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAAc                                                                                         <  2:1696572/150‑1 (MQ=255)
                                                              atatggACCAGCGCCATCCGCACTGCTACTTTGGCCCATGCACCCGCACGTACTCCGCCGTCGAGATCGTCACGTACTAATCCAAACCCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACg                                                                                        <  2:1907563/145‑1 (MQ=255)
                                                              aGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACg                                                                                        >  1:1002159/1‑150 (MQ=255)
                                                                aTTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGCCTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCt                                                                                      <  2:2205538/150‑1 (MQ=255)
                                                                       cgcgcAATCCGCACTGATACTTTTGCCCATGCATCCGCACGTACTTCGCCGTCGTGATCGCCAAGTAATAATTAAAAACCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGaa                                                                               <  1:1181664/149‑1 (MQ=255)
                                                                         cgcCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAg                                                                             >  1:2287037/1‑150 (MQ=255)
                                                                           ccAAACGCACTGCTTCTTTCCCCCATCCAGCCGCACGTACTGCGCCGTCGTGATCGGAATGCAATAATAAATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGAc                                                                           <  2:4474427/150‑1 (MQ=255)
                                                                            cATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACg                                                                          >  1:2495181/1‑150 (MQ=255)
                                                                             aTACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGc                                                                         >  1:300299/1‑150 (MQ=255)
                                                                             aTACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGc                                                                         >  1:505858/1‑150 (MQ=255)
                                                                             aTACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGc                                                                         >  1:1466282/1‑150 (MQ=255)
                                                                                  caatGCTTCATTTTCCAATGCATCCGCACGGACTTCGCCGTCTAGATCGGCAAGGAAGAATTCATAACCAAAACGAACTCCTCCTCAACTTTCACCTCGTTCTCGTTTGCAATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAg                                                                    >  2:437811/4‑150 (MQ=255)
                                                                                  cACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAg                                                                    >  2:156882/1‑150 (MQ=255)
                                                                                   aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                   >  2:1268106/1‑150 (MQ=255)
                                                                                   aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                   >  1:141181/1‑150 (MQ=255)
                                                                                   aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                   >  1:1304701/1‑150 (MQ=255)
                                                                                   aCTGCTTCTTTTGCCAATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                   >  2:3383901/1‑150 (MQ=255)
                                                                                    cTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcg                                                                  >  1:257358/1‑150 (MQ=255)
                                                                                     tGCTTCTTTTGNCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                 >  1:2764868/1‑150 (MQ=255)
                                                                                     tGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                 >  1:3480726/1‑150 (MQ=255)
                                                                                     tGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                 >  2:5084670/1‑150 (MQ=255)
                                                                                     tGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACAGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                 >  2:3029081/1‑150 (MQ=255)
                                                                                      gCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgga                                                                >  2:4555807/1‑150 (MQ=255)
                                                                                      gCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgga                                                                >  1:1043866/1‑150 (MQ=255)
                                                                                       cttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggac                                                               >  1:3938087/1‑150 (MQ=255)
                                                                                        ttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacg                                                              >  1:5152729/1‑150 (MQ=255)
                                                                                             ttGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACAt                                                         <  2:4763698/150‑1 (MQ=255)
                                                                                              tGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATc                                                        >  2:1252167/1‑150 (MQ=255)
                                                                                                  cATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgcc                                                    >  2:1472866/1‑150 (MQ=255)
                                                                                                  cATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgcc                                                    >  2:3114687/1‑150 (MQ=255)
                                                                                                   aTGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgccg                                                   >  2:369018/1‑150 (MQ=255)
                                                                                                     gCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCt                                                 >  2:1658061/1‑150 (MQ=255)
                                                                                                     gCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCt                                                 >  1:2588351/1‑150 (MQ=255)
                                                                                                       aTCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAg                                               >  2:4428265/1‑150 (MQ=255)
                                                                                                        tCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGc                                              >  2:347210/1‑150 (MQ=255)
                                                                                                        tCCGCACGGACTTCGCCGTCGAGAGCGGCAAGGAAGAATTCAAACCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGc                                              <  2:296696/150‑1 (MQ=255)
                                                                                                            cACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTCGCGTGc                                          >  2:3304188/1‑150 (MQ=255)
                                                                                                               ggACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGt                                       >  1:4658761/1‑150 (MQ=255)
                                                                                                                gACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTc                                      >  2:1374004/1‑150 (MQ=255)
                                                                                                                 aCTTCGCCGTCGCGATCGGCATGGAAGAATTCATACCCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCg                                     <  2:5075475/150‑1 (MQ=255)
                                                                                                                 aCTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCg                                     >  2:2306229/1‑150 (MQ=255)
                                                                                                                 aCTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCg                                     >  1:3070467/1‑150 (MQ=255)
                                                                                                                    tCGCCGGCGAGATCGGCACGGACTAATTCATACCCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTg                                  <  2:2788775/150‑1 (MQ=255)
                                                                                                                      gCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGc                                >  2:1501448/1‑150 (MQ=255)
                                                                                                                      gCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGc                                <  2:160293/150‑1 (MQ=255)
                                                                                                                       ccGACGAGATCGGCAAGGAAGAATTCATACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGcc                               <  2:5288050/150‑1 (MQ=255)
                                                                                                                        cGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCTGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCg                              >  2:1348419/1‑150 (MQ=255)
                                                                                                                        cGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCg                              >  1:3735065/1‑150 (MQ=255)
                                                                                                                         gTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGc                             >  1:610184/1‑150 (MQ=255)
                                                                                                                         gTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGc                             >  2:290103/1‑150 (MQ=255)
                                                                                                                          acgcgaTCGGCATGGAATAATAAATACCCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCa                            <  1:2932023/146‑1 (MQ=255)
                                                                                                                            gcgcgcGGCATGGAAGAATTCATCCCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                          <  2:3761299/145‑1 (MQ=255)
                                                                                                                            gagaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                          >  1:4455865/1‑150 (MQ=255)
                                                                                                                            gagaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                          <  2:2573653/150‑1 (MQ=255)
                                                                                                                            gagaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                          >  2:2438568/1‑150 (MQ=255)
                                                                                                                             agaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCCAcc                         >  2:4320471/1‑150 (MQ=255)
                                                                                                                             agaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAcc                         >  2:1852519/1‑150 (MQ=255)
                                                                                                                             agaTCGGCAAGGAAGAATTCAAAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAcc                         <  2:4007444/150‑1 (MQ=255)
                                                                                                                                tCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCaaa                      <  2:3806455/150‑1 (MQ=255)
                                                                                                                                    catggAATAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaatta                  <  2:1043892/147‑1 (MQ=255)
                                                                                                                                     aaGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaa                 >  1:4287501/1‑150 (MQ=255)
                                                                                                                                      aGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaat                <  2:4825051/150‑1 (MQ=255)
                                                                                                                                        gaagaaTTCATAACCACCACGCCCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTc              <  2:1154010/150‑1 (MQ=255)
                                                                                                                                          agaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCAt            <  2:3420577/150‑1 (MQ=255)
                                                                                                                                           gaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATa           >  1:3436764/1‑150 (MQ=255)
                                                                                                                                            aaTTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAg          <  2:523357/150‑1 (MQ=255)
                                                                                                                                            aaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAg          >  1:813835/1‑150 (MQ=255)
                                                                                                                                              ttCATAACCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGac        <  1:2048655/150‑1 (MQ=255)
                                                                                                                                                   aaCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGAcaccac   <  2:4344647/150‑1 (MQ=255)
                                                                                                                                                   aaCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGAcaccac   <  2:1025891/150‑1 (MQ=255)
                                                                                                                                                    aCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACt  >  2:2619874/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACT  >  CP009273/3384613‑3384910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑