Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,384,766 A→C 19.8% F208V (TTT→GTT)  tldD ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,384,7660AC19.8% 514.1 / 60.9 266F208V (TTT→GTT) tldDputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (176/32);  new base C (2/56);  total (178/88)
Fisher's exact test for biased strand distribution p-value = 6.95e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

GGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGG  >  CP009273/3384618‑3384914
                                                                                                                                                    |                                                                                                                                                    
    aaCCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGACGAATTCATAACCAAAACGAc                                                                                                                                                 >  1:67760/1‑150 (MQ=255)
    aaCCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAc                                                                                                                                                 >  2:3162069/1‑150 (MQ=255)
           gcCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAccgccgcc                                                                                                                                          >  2:1064192/1‑150 (MQ=255)
           gcCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAccgccgcc                                                                                                                                          >  1:5099150/1‑150 (MQ=255)
             cAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACcgccgccgc                                                                                                                                        >  2:2048655/1‑150 (MQ=255)
             cAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACcgccgccgc                                                                                                                                        >  2:2622951/1‑150 (MQ=255)
               aGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCa                                                                                                                                      >  2:4063562/1‑150 (MQ=255)
                   ctacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTgg                                                                                                                                  >  1:2986654/1‑150 (MQ=255)
                    tacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGc                                                                                                                                 >  1:305558/1‑150 (MQ=255)
                          cATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcg                                                                                                                           >  1:4161065/1‑150 (MQ=255)
                             ggTGCCCGCTGGTGCTTCAAGTGATTAATGATTGACCTGCGCCATACCAACTGATTCTTTGGCCCAGGCATCCGCACGGACTTCGCCGTGTATCTGGGCTTGTTTTAATACATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttc                                                                                                                        <  2:5191633/150‑1 (MQ=255)
                                  ccGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcgcgtt                                                                                                                   >  1:3304799/1‑150 (MQ=255)
                                      tGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGcc                                                                                                               >  2:1079877/1‑150 (MQ=255)
                                      tGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGcc                                                                                                               >  2:2550212/1‑150 (MQ=255)
                                         tGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtc                                                                                                            >  1:523357/1‑150 (MQ=255)
                                           aagcaACGGCATAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGAATCCGCACGGACTTCGCCGTCTAGATCGGCAAGTAATAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtctt                                                                                                          <  2:1681715/149‑1 (MQ=255)
                                             gcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtcttct                                                                                                        >  2:893276/1‑150 (MQ=255)
                                              caACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCTCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATcttctt                                                                                                       >  1:2236553/1‑150 (MQ=255)
                                               aaCGGCTGAAAGATTGACCTGCGCCAACCGCACTGCTTCTTTTGCCCATGCATCCGCCCGGCCTTCGCCGTCGAGATCGGCAAGGAAGAATACATACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCttcttc                                                                                                      <  2:1792420/150‑1 (MQ=255)
                                               aaCGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCttcttc                                                                                                      >  1:1777455/1‑150 (MQ=255)
                                                  ggCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgac                                                                                                   >  1:4275055/1‑150 (MQ=255)
                                                   gCAGAAAGAGTGACCCGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCCCGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCACAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgacg                                                                                                  <  2:142294/150‑1 (MQ=255)
                                                     aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                                >  1:1455669/1‑150 (MQ=255)
                                                     aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                                >  2:1175373/1‑150 (MQ=255)
                                                     aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                                >  2:744981/1‑150 (MQ=255)
                                                      gAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgaga                                                                                               >  2:295738/1‑150 (MQ=255)
                                                      gAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgaga                                                                                               >  1:901599/1‑150 (MQ=255)
                                                        attaTTGACCCGCCCCATCCGCACTGCTTCTTTTGCCCATGAATCCGCACGGACTTCGCCGTCGATATCGGCAAGGAATAATTAAAAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAAc                                                                                             <  1:3084415/147‑1 (MQ=255)
                                                        aaGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAAc                                                                                             <  2:5311144/150‑1 (MQ=255)
                                                        aaGATTCACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTGCGCCGTCTCTCTCGGCATGTAATAATTCAAAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAAc                                                                                             <  1:2911582/150‑1 (MQ=255)
                                                         aGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACg                                                                                            >  1:1002159/1‑150 (MQ=255)
                                                         aGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACg                                                                                            >  1:2309217/1‑150 (MQ=255)
                                                         aGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACg                                                                                            >  1:1473959/1‑150 (MQ=255)
                                                           aTTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGCCTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCt                                                                                          <  2:2205538/150‑1 (MQ=255)
                                                              gACCAGCGCCATCCGCACTGCTTATTTGGCCCATGAATCCGCACGGACTTCGCCGTCGAGATGGGCACGTAAGAATACATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCAc                                                                                       <  2:1924737/150‑1 (MQ=255)
                                                                  cgcgcAATCCGCACTGATACTTTTGCCCATGCATCCGCACGTACTTCGCCGTCGTGATCGCCAAGTAATAATTAAAAACCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGaa                                                                                   <  1:1181664/149‑1 (MQ=255)
                                                                     gcCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGa                                                                                >  2:508145/1‑150 (MQ=255)
                                                                      ccacccGCACGTCTTCTTTTCCCCAGGCATCCGCACGGACTTCGCCGTCTTGATCGGCAAGTAATAATAAATAACCCACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGAc                                                                               <  2:4474433/145‑1 (MQ=255)
                                                                      caaaccGCACTGTTTCTTTGCCCCTTGCACCCGCCCGGACTTCGCCGTCGAGACCGCAATGGAAGATTACAAACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGAc                                                                               <  2:4474403/145‑1 (MQ=255)
                                                                        agaCGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCACGGACGACTTCAGAACCAACACGACCGCCGCCGCCACTGGCACCGCGTGCGCGTGTGCCATCTTCTTCGACGAGAACGCTCACGGAACGACGc                                                                             >  2:21353/3‑150 (MQ=255)
                                                                           cGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCAcc                                                                          >  2:4332596/1‑150 (MQ=255)
                                                                            gCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTAGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCa                                                                         >  1:1289071/1‑150 (MQ=255)
                                                                              aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                       >  2:1184575/1‑150 (MQ=255)
                                                                              aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                       >  2:1268106/1‑150 (MQ=255)
                                                                              aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                       >  1:2091554/1‑150 (MQ=255)
                                                                              aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                       >  2:157826/1‑150 (MQ=255)
                                                                              aCTGCTTCTTTTGCCAATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                       >  2:3383901/1‑150 (MQ=255)
                                                                                tGCTTCTTTTGNCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                     >  1:2764868/1‑150 (MQ=255)
                                                                                tGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                     >  1:3480726/1‑150 (MQ=255)
                                                                                tGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                     >  1:1998044/1‑150 (MQ=255)
                                                                                tGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACAGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgg                                                                     >  2:3029081/1‑150 (MQ=255)
                                                                                 gCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgga                                                                    >  1:1043866/1‑150 (MQ=255)
                                                                                 gCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgga                                                                    >  2:4568996/1‑150 (MQ=255)
                                                                                  cttcttTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggac                                                                   >  1:3938087/1‑150 (MQ=255)
                                                                                        ttGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACAt                                                             <  2:4763698/150‑1 (MQ=255)
                                                                                             cATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgcc                                                        >  2:3114687/1‑150 (MQ=255)
                                                                                             cATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgcc                                                        >  2:435407/1‑150 (MQ=255)
                                                                                              aTGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgccg                                                       >  2:369018/1‑150 (MQ=255)
                                                                                                   tCCGCACGGACTTCGCCGTCTCGATCGGCAAGGAATAATACAAAACCAACCCGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGc                                                  <  2:67760/150‑1 (MQ=255)
                                                                                                   tCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGc                                                  >  2:347210/1‑150 (MQ=255)
                                                                                                       cACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTCGCGTGc                                              >  2:3304188/1‑150 (MQ=255)
                                                                                                        aCGTACTTCGCAGTCTAGATCGTAAATTAATAATACAAAACCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGcc                                             <  1:1425159/150‑1 (MQ=255)
                                                                                                          ggACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGt                                           >  1:4658761/1‑150 (MQ=255)
                                                                                                           gACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTc                                          >  2:1374004/1‑150 (MQ=255)
                                                                                                            aCTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCg                                         >  2:2306229/1‑150 (MQ=255)
                                                                                                            aCTCCGCCGTCTAGATCGGCACGTAATAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCg                                         <  1:2583225/150‑1 (MQ=255)
                                                                                                               tCGCCGGCGAGATCGGCACGGACTAATTCATACCCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTg                                      <  2:2788775/150‑1 (MQ=255)
                                                                                                                 gCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGc                                    >  2:1501448/1‑150 (MQ=255)
                                                                                                                   cGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCTGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCg                                  >  2:1348419/1‑150 (MQ=255)
                                                                                                                   cGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCg                                  >  1:3496437/1‑150 (MQ=255)
                                                                                                                    gTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGc                                 >  2:290103/1‑150 (MQ=255)
                                                                                                                    gTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGc                                 >  1:610184/1‑150 (MQ=255)
                                                                                                                      cGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCac                               >  1:3433180/1‑149 (MQ=255)
                                                                                                                       gcgcgcGGCATGGAAGAATTCATCCCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                              <  2:3761299/145‑1 (MQ=255)
                                                                                                                       gagaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                              >  2:2438568/1‑150 (MQ=255)
                                                                                                                       gagaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                              >  1:4350524/1‑150 (MQ=255)
                                                                                                                           tCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCaaa                          <  2:3806455/150‑1 (MQ=255)
                                                                                                                            cGGCACGGAAGAATTCATACCCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCaaaa                         <  2:1618121/150‑1 (MQ=255)
                                                                                                                             ggCCAGGAAGAATTCAGACCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCCACCAAaat                        >  2:4279855/1‑150 (MQ=255)
                                                                                                                             ggCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaat                        >  1:4523260/1‑150 (MQ=255)
                                                                                                                             ggCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaat                        >  2:3973854/1‑150 (MQ=255)
                                                                                                                               catggAATAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaatta                      <  2:1043892/147‑1 (MQ=255)
                                                                                                                                aaGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaa                     >  2:4385373/1‑150 (MQ=255)
                                                                                                                                 aGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaat                    <  2:4825051/150‑1 (MQ=255)
                                                                                                                                  ggAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaatt                   <  2:1766574/150‑1 (MQ=255)
                                                                                                                                   gaagaaTTCATAACCACCACGCCCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTc                  <  2:1154010/150‑1 (MQ=255)
                                                                                                                                    aagaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCa                 >  2:258091/1‑150 (MQ=255)
                                                                                                                                     agaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCAt                <  2:3420577/150‑1 (MQ=255)
                                                                                                                                      gaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATa               >  1:2522017/1‑150 (MQ=255)
                                                                                                                                        aTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGa             >  1:5011442/1‑150 (MQ=255)
                                                                                                                                         ttCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGac            <  2:5246886/150‑1 (MQ=255)
                                                                                                                                         taaaaacccAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGac            <  1:1385471/143‑1 (MQ=255)
                                                                                                                                              aaCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGAcaccac       >  1:698604/1‑150 (MQ=255)
                                                                                                                                               cccAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACt      <  2:1558309/149‑1 (MQ=255)
                                                                                                                                               aCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACt      >  1:36942/1‑150 (MQ=255)
                                                                                                                                                 cAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGa    <  1:1856857/150‑1 (MQ=255)
                                                                                                                                                 cAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGa    >  2:5021741/1‑150 (MQ=255)
                                                                                                                                                 cAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGa    >  2:2693396/1‑150 (MQ=255)
                                                                                                                                                  anaaCGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGACATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCACCCCAAATTAAGGCATAGACCCCACTGAg   >  1:122103/1‑150 (MQ=255)
                                                                                                                                                   aaaCGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAgg  >  2:4681269/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGG  >  CP009273/3384618‑3384914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑