Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,402,321 C→A 13.6% I452I (ATC→ATA)  panF → pantothenate:sodium symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,402,3210CA13.6% 959.7 / 81.0 301I452I (ATC→ATA) panFpantothenate:sodium symporter
Reads supporting (aligned to +/- strand):  ref base C (104/156);  new base A (14/27);  total (118/183)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 314 total aligned reads displayed.

CTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAACACCACCGGCGCGAAC  >  CP009273/3402172‑3402470
                                                                                                                                                     |                                                                                                                                                     
cTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATc                                                                                                                                                       <  1:1605708/150‑1 (MQ=255)
cTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATc                                                                                                                                                       >  1:4302504/1‑150 (MQ=255)
cTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATc                                                                                                                                                       <  1:4393344/150‑1 (MQ=255)
cTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATc                                                                                                                                                       >  1:5113542/1‑150 (MQ=255)
cTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATc                                                                                                                                                       >  2:1300179/1‑150 (MQ=255)
  ggAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGt                                                                                                                                                     >  2:4766267/1‑150 (MQ=255)
       ccGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCt                                                                                                                                                <  1:3450345/150‑1 (MQ=255)
        cGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTc                                                                                                                                               <  2:911876/150‑1 (MQ=255)
        cGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTc                                                                                                                                               <  2:4493603/150‑1 (MQ=255)
        cGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTc                                                                                                                                               <  1:4005799/150‑1 (MQ=255)
         gTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCg                                                                                                                                              >  1:121694/1‑150 (MQ=255)
         gTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCg                                                                                                                                              <  1:1521531/150‑1 (MQ=255)
               cTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTtacta                                                                                                                                        >  2:2371198/1‑150 (MQ=255)
               cTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGAGATAAGTGCGATGATCGTTGTCGGCGTGCTGTATGCCGTACTAGCGACGCTGAATATTCAGTACCTGGGCTTCCACCATCTAGTGCCCTCGTtacta                                                                                                                                        >  2:5019505/1‑150 (MQ=255)
                 gtgGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTactact                                                                                                                                      <  2:3163644/150‑1 (MQ=255)
                     ccGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGt                                                                                                                                  <  2:705421/150‑1 (MQ=255)
                     ccGCTGGTGCGGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGt                                                                                                                                  <  2:1815345/150‑1 (MQ=255)
                      cGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGtt                                                                                                                                 <  2:5041545/150‑1 (MQ=255)
                       gCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGttt                                                                                                                                >  2:2920179/1‑150 (MQ=255)
                          ggTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCt                                                                                                                             >  1:2567262/1‑150 (MQ=255)
                          ggTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCt                                                                                                                             <  1:1140750/150‑1 (MQ=255)
                            tGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTCAGTGTGCTgg                                                                                                                           >  2:963220/1‑150 (MQ=255)
                            tGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTgg                                                                                                                           <  2:2599079/150‑1 (MQ=255)
                             gCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGc                                                                                                                          >  2:1071453/1‑150 (MQ=255)
                             gCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGc                                                                                                                          <  1:5187968/150‑1 (MQ=255)
                              cTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCt                                                                                                                         <  1:2024560/150‑1 (MQ=255)
                               tGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCtt                                                                                                                        <  1:3493219/150‑1 (MQ=255)
                               tGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCtt                                                                                                                        >  2:2299272/1‑150 (MQ=255)
                                gggTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCttt                                                                                                                       <  1:2219002/150‑1 (MQ=255)
                                   tCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCt                                                                                                                    >  1:4797475/1‑150 (MQ=255)
                                       tACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTc                                                                                                                <  2:4210143/150‑1 (MQ=255)
                                           gggAACGCGCCAACGCCAACGGCGCGCTCAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTGCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGaa                                                                                                            <  2:365429/150‑1 (MQ=255)
                                           gggAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGaa                                                                                                            >  1:2648759/1‑150 (MQ=255)
                                           gggAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGaa                                                                                                            <  2:2537644/150‑1 (MQ=255)
                                             gAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAAc                                                                                                          >  1:58726/1‑150 (MQ=255)
                                               aCGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCg                                                                                                        <  2:435810/150‑1 (MQ=255)
                                                  cgccaacgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGttt                                                                                                     <  2:4070023/150‑1 (MQ=255)
                                                     caacgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCgg                                                                                                  >  1:212174/1‑150 (MQ=255)
                                                      aacgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGt                                                                                                 <  2:5164745/150‑1 (MQ=255)
                                                        cgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTAc                                                                                               <  1:3253702/150‑1 (MQ=255)
                                                            aaaGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATcc                                                                                           >  1:4541783/1‑150 (MQ=255)
                                                            aaaGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATcc                                                                                           >  2:2968067/1‑150 (MQ=255)
                                                            aaaGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATcc                                                                                           >  1:4104654/1‑150 (MQ=255)
                                                            aaaGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATcc                                                                                           <  1:5005318/150‑1 (MQ=255)
                                                            aaaGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATcc                                                                                           >  1:1454554/1‑150 (MQ=255)
                                                            aaaGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATcc                                                                                           <  1:4072315/150‑1 (MQ=255)
                                                            aaaGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATAAACAGAACCTGCGCTACCACCCTATCGTGCCCTCGTTACTACTAATTATGCTGGCTTTACTGGTCGGAAACCGTTACGGAAAATcc                                                                                           >  1:1013119/1‑150 (MQ=255)
                                                                 cgcgcTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTccc                                                                                      <  2:3071975/150‑1 (MQ=255)
                                                                     cTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCaa                                                                                  >  1:2009608/1‑150 (MQ=255)
                                                                       aaGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGc                                                                                >  1:872611/1‑150 (MQ=255)
                                                                           gCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTAcc                                                                            <  2:1186355/150‑1 (MQ=255)
                                                                              atgatCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGtt                                                                         <  1:534791/150‑1 (MQ=255)
                                                                              atgatCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGtt                                                                         <  1:352144/150‑1 (MQ=255)
                                                                              atgatCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGtt                                                                         <  1:1331703/150‑1 (MQ=255)
                                                                              aggatCGTTGGCGGCGAGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTGCCCCCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGtt                                                                         <  2:1716797/148‑1 (MQ=255)
                                                                                 atCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTg                                                                      <  1:376611/150‑1 (MQ=255)
                                                                                 atCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTg                                                                      <  2:3881234/150‑1 (MQ=255)
                                                                                   cGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGac                                                                    <  1:2342892/150‑1 (MQ=255)
                                                                                   cGTTGGCGGAGTGCTGTATGCCGTACTCGCGACTCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTAGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGac                                                                    <  1:1875931/150‑1 (MQ=255)
                                                                                    gTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGact                                                                   >  2:2421138/1‑150 (MQ=255)
                                                                                     ttGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGacta                                                                  >  2:2217939/1‑150 (MQ=255)
                                                                                     ttGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGacta                                                                  <  1:3536236/150‑1 (MQ=255)
                                                                                       ggcggcGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGactact                                                                <  1:3166834/150‑1 (MQ=255)
                                                                                       ggcggcGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGactact                                                                >  1:3408077/1‑150 (MQ=255)
                                                                                         cggcgTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGa                                                              >  2:3322754/1‑150 (MQ=255)
                                                                                          ggcgTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGat                                                             <  1:4766267/150‑1 (MQ=255)
                                                                                              tGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGataaat                                                         <  1:88043/150‑1 (MQ=255)
                                                                                                cTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGataaataa                                                       <  2:5318465/150‑1 (MQ=255)
                                                                                                 tGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGAtaaataaa                                                      >  2:2167389/1‑150 (MQ=255)
                                                                                                     tGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGt                                                  <  1:2178521/150‑1 (MQ=255)
                                                                                                       ccGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGttt                                                <  1:4830636/150‑1 (MQ=255)
                                                                                                       ccGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGttt                                                <  2:1569311/150‑1 (MQ=255)
                                                                                                        cGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGtttt                                               <  2:1363148/150‑1 (MQ=255)
                                                                                                         gTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTg                                              >  2:541393/1‑150 (MQ=255)
                                                                                                            cTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCa                                           <  2:4188876/150‑1 (MQ=255)
                                                                                                                 gACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCt                                      >  1:4901007/1‑150 (MQ=255)
                                                                                                                  aCGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCtt                                     <  1:3568667/150‑1 (MQ=255)
                                                                                                                   cGCTGAATATTCAGTACCTGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTg                                    <  1:7565/150‑1 (MQ=255)
                                                                                                                      tGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGAt                                 <  1:3049128/150‑1 (MQ=255)
                                                                                                                       gAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATc                                <  2:1654230/150‑1 (MQ=255)
                                                                                                                        aaTATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATcc                               >  2:3414684/1‑150 (MQ=255)
                                                                                                                        aaTATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGGTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATcc                               >  2:2119911/1‑150 (MQ=255)
                                                                                                                           atTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCaac                            >  2:2890042/1‑150 (MQ=255)
                                                                                                                            ttCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCaact                           <  2:4723892/150‑1 (MQ=255)
                                                                                                                             tCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCaactg                          >  2:3640975/1‑150 (MQ=255)
                                                                                                                                 tACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCaactgaaac                      >  2:600657/1‑150 (MQ=255)
                                                                                                                                 tACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCaactgaaac                      <  2:4898586/150‑1 (MQ=255)
                                                                                                                                  aCCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCaactgacact                     >  1:3001503/1‑150 (MQ=255)
                                                                                                                                   ccTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCaactgaaactg                    >  1:1698038/1‑150 (MQ=255)
                                                                                                                                     tGGGCTTCCACCCTATAGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAactgaaactgaa                  >  1:4636325/1‑150 (MQ=255)
                                                                                                                                       ggCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAaca                <  2:4088254/150‑1 (MQ=255)
                                                                                                                                        gCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAacac               >  2:2479186/1‑150 (MQ=255)
                                                                                                                                         cTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAAcacc              >  1:4218469/1‑150 (MQ=255)
                                                                                                                                           tCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAATCTGAAcaccac            <  1:2734056/150‑1 (MQ=255)
                                                                                                                                           tCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAAcaccac            <  2:3560424/150‑1 (MQ=255)
                                                                                                                                            ccACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAACaccacc           >  2:3804419/1‑150 (MQ=255)
                                                                                                                                                 cTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAACACCACCGgcgc      <  2:1974627/150‑1 (MQ=255)
                                                                                                                                                   aTCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAACACCACCGGCGCGa    >  1:4058016/1‑150 (MQ=255)
                                                                                                                                                   aTCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAACACCACCGGCGCGa    >  2:3517429/1‑150 (MQ=255)
                                                                                                                                                    tCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAACACCACCGGCGCGaa   >  2:3722954/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCCATGCCTTGGATCCAACTGAAACTGAACACCACCGGCGCGAAC  >  CP009273/3402172‑3402470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑